Perfect Anti-Human CD3

February 19, 2016

Neurosurgery
BWH/HMS
postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-human CD3 PE-Cyanine5.5

Catalog Number:

35-0036

This antibody was used to investigate the amount of T cells after PBMCs extraction. The results were perfect and the optimization really easy.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACSCalibur (Becton Dickinson)

Results Summary

The sample was incubated for 30min with CD3, washed twice and then re-suspended in washing buffer for the analysis. The antibody was tested at multiple concentrations, from pure to 1:100 and the staining was perfect in all the cases.

Additional Notes

None

Related Categories

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Summary

The Good

Perfect clear staining

The Bad

Price

The Bottom Line

This is a very good anti-CD3 antibody.

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