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Author Profile
Mikyoung You
Postdoctoral Researcher
UNC NRI
Articles by Mikyoung You
Assay Kit/Reagent User Review
Working Great for Proliferation
July 03, 2023
I used Click-iT plus EdU imaging kits to determine proliferation in human cancer cells. It works very well and is easy to handle.
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Assay Kit/Reagent User Review
Phalloidin Kit Works Great
July 02, 2023
I used a Phalloidin kit from Abcam to check fibroblast morphology. It is very easy to handle and the results look great.
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Assay Kit/Reagent User Review
Working Great in Fibroblasts
July 02, 2023
I used a Chloroquine diphosphate salt from Sigma-Aldrich to block autophagosome degradation. I found this one which we purchased ...
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Antibody User Review
p-AMPK Antibody Works Well
December 27, 2022
We measured the p-AMPK level in HFD-fed mouse liver. 20ug of liver lysate was applied and p-AMPK was incubated overnight.
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Antibody User Review
High Quality AMPK Antibody
December 19, 2022
The AMPK expression was measured in the mouse liver which fed a high-fat diet (60% Kcal fat) for 13 weeks.
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Antibody User Review
LC3 Antibody Works Well
December 16, 2022
25mg mouse liver lysate was applied into 10% SDS-page and incubated with LC3 antibody (1:1,000, in 5% BSA) overnight.
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Antibody User Review
IgG Antibody Works Well
September 08, 2022
I use this one for the secondary antibody of Western blot. It is good with 1:5,000 dilution for 2 hours incubation at room ...
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Antibody User Review
VDAC1 Antibody Works Well
September 07, 2022
To check the mitochondrial fraction, we used VDAC as a reference protein. This antibody is working well in human fibroblasts. Very...
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Assay Kit/Reagent User Review
Good Quality Blocker
September 07, 2022
I use BSA in PBS reagent for immunofluorescence staining as a blocking solution. This one is very easy to use and has worked great...
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Assay Kit/Reagent User Review
DNA-Free Kit Works Well
September 07, 2022
After the isolation of mRNA from animal tissue, I use this regularly. When the 260/280 ratio in RNA quantification is low than 1.8...
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Antibody User Review
Good Quality OXPHOS WB Antibody
September 06, 2022
The total OXPHOS Rodent WV antibody works great in white adipose tissue in B6 mice. The denaturation step is 37C for 30min ...
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Assay Kit/Reagent User Review
Good QC Fluorospheres for FACS
June 03, 2022
We are using CytoFLEX Flow Cytometer for cell sorting and cell cycle. This machine has two types of sample loading; plate and tube...
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Assay Kit/Reagent User Review
Good Protease Inhibitor
June 02, 2022
We used this protease inhibitor for western blot. Each tube has 100uL and 100X dilution factor with RIPA buffer. Easy to use. ...
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Assay Kit/Reagent User Review
Good Mutagenesis Kit
June 02, 2022
The research goal is to check protein activity in normal or mutated proteins. This kit is used to generate mutation at specific ...
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Assay Kit/Reagent User Review
Excellent RIPA Buffer
June 01, 2022
I've used this one for over 10 years. Working is really well for cells and animal tissues. After 30 min incubation in ice, fat is ...
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Product Review
Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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