Mikyoung You

Postdoctoral Researcher
UNC NRI

Articles by Mikyoung You

  • Working Great for Proliferation

    July 03, 2023
    I used Click-iT plus EdU imaging kits to determine proliferation in human cancer cells. It works very well and is easy to handle. read more

  • Phalloidin Kit Works Great

    July 02, 2023
    I used a Phalloidin kit from Abcam to check fibroblast morphology. It is very easy to handle and the results look great. read more

  • Working Great in Fibroblasts

    July 02, 2023
    I used a Chloroquine diphosphate salt from Sigma-Aldrich to block autophagosome degradation. I found this one which we purchased ... read more

  • p-AMPK Antibody Works Well

    December 27, 2022
    We measured the p-AMPK level in HFD-fed mouse liver. 20ug of liver lysate was applied and p-AMPK was incubated overnight. read more

  • High Quality AMPK Antibody

    December 19, 2022
    The AMPK expression was measured in the mouse liver which fed a high-fat diet (60% Kcal fat) for 13 weeks. read more

  • LC3 Antibody Works Well

    December 16, 2022
    25mg mouse liver lysate was applied into 10% SDS-page and incubated with LC3 antibody (1:1,000, in 5% BSA) overnight. read more

  • IgG Antibody Works Well

    September 08, 2022
    I use this one for the secondary antibody of Western blot. It is good with 1:5,000 dilution for 2 hours incubation at room ... read more

  • VDAC1 Antibody Works Well

    September 07, 2022
    To check the mitochondrial fraction, we used VDAC as a reference protein. This antibody is working well in human fibroblasts. Very... read more

  • Good Quality Blocker

    September 07, 2022
    I use BSA in PBS reagent for immunofluorescence staining as a blocking solution. This one is very easy to use and has worked great... read more

  • DNA-Free Kit Works Well

    September 07, 2022
    After the isolation of mRNA from animal tissue, I use this regularly. When the 260/280 ratio in RNA quantification is low than 1.8... read more

  • Good Quality OXPHOS WB Antibody

    September 06, 2022
    The total OXPHOS Rodent WV antibody works great in white adipose tissue in B6 mice. The denaturation step is 37C for 30min ... read more

  • Good QC Fluorospheres for FACS

    June 03, 2022
    We are using CytoFLEX Flow Cytometer for cell sorting and cell cycle. This machine has two types of sample loading; plate and tube... read more

  • Good Protease Inhibitor

    June 02, 2022
    We used this protease inhibitor for western blot. Each tube has 100uL and 100X dilution factor with RIPA buffer. Easy to use. ... read more

  • Good Mutagenesis Kit

    June 02, 2022
    The research goal is to check protein activity in normal or mutated proteins. This kit is used to generate mutation at specific ... read more

  • Excellent RIPA Buffer

    June 01, 2022
    I've used this one for over 10 years. Working is really well for cells and animal tissues. After 30 min incubation in ice, fat is ... read more
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