Good Quality OXPHOS WB Antibody

September 06, 2022

Nutrition
UNC NRI
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Total OXPHOS WB antibody

Catalog Number:

ab110413

Clone Number:

N/A

The total OXPHOS Rodent WV antibody works great in white adipose tissue in B6 mice. The denaturation step is 37C for 30min instead of 95C for 10min. The dilution ratio is 1:250 in the BSA solution.

Experimental Design and Results Summary

Applications

Western Blot

Sample

White adipose tissue

Primary Incubation

1: 250 dilution for overnight

Blocking Agent

5% BSA

Secondary Incubation

1:2000, 1 hour, room temperature

Tertiary Incubation

30sec

Detection

Chemiluminesence

Results Summary

We expected that the treated group shows higher expression of OXPHOS than the control group. Results are great to see our expectations. Five component is well separated in 10% SDS-PAGE.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Accessible and high quality.

The Bad

Kind of expensive.

The Bottom Line

Expensive but we would like to buy it always.

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