p-AMPK Antibody Works Well

December 27, 2022

Nutrition
UNC NRI
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

p-AMPK antibody

Catalog Number:

2535

Clone Number:

N/A

Info:Supplier Page View Product Specs

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We measured the p-AMPK level in HFD-fed mouse liver. 20ug of liver lysate was applied and p-AMPK was incubated overnight.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse liver

Primary Incubation

1:1000, overnight, refrigerator

Blocking Agent

5% BSA

Secondary Incubation

1:2000, 1 hour, room temperature

Tertiary Incubation

30 sec

Detection

Chemiluminesence

Results Summary

The p-AMPK band is detected clearly. The level of p-AMPK was increased in treated groups (lane 3 and 4) compared to control (lane 2).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Accessible and high quality.

The Bad

Expensive

The Bottom Line

Antibody has great signal, highly recommended.

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