Abcam
VDAC1/Porin antibody
ab186321
N/A
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To check the mitochondrial fraction, we used VDAC as a reference protein. This antibody is working well in human fibroblasts. Very clearly signal and is easy to handle.
Western Blot
Cells
1:1000, overnight, refrigerator
3% skim milk
1:2000, 1 hour, room temperature
30sec
Chemiluminesence
Good and pretty quality results. We didn't get results using a different antibody but, this one is very good. We always re-used it for a week and work is well too.
Reliable and good signal.
The antibody is a great signal, highly recommended.