Working Great in Fibroblasts

UNC NRI
Nutrition
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Chloroquine diphosphate salt

Catalog Number:

C6628

I used a Chloroquine diphosphate salt from Sigma-Aldrich to block autophagosome degradation. I found this one which we purchased in 2010, it is still working very well.

Experimental Design and Results Summary

Application

Fibroblasts

Starting Material

Tips

I used 10 uM chloroquine for 15 hours.

Results Summary

The cells with chloroquine treatment showed significantly higher LC3-II expression compared to cells without any treatment.

DOI or PMID #

N/A

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Summary

The Good

Accessible and high quality.

The Bad

None.

The Bottom Line

This one has great results.

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