
Enzymes, or biological catalysts, are some of the most widely manipulated reagents in molecular biology and biochemistry research and diagnostics. The appropriate enzymatic reagent depends on the type of enzyme, target molecule, final reaction product, reagent grade, and working volume. Peptidases, enzymes that initiate protein catabolism by hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain, DNA glycosylases, the enzymes involved in base excision repair, and kinases, enzymes that transfers phosphate groups from high-energy donor molecules, such as ATP to specific substrates, are examples of available enzymes from suppliers such as New England Biolabs®, Promega, and Roche. DNA experiments use nucleic acid-specific enzymes, such as restriction enzymes which cut DNA, ligases which join DNA bases, and topoisomerases which uncoil DNA. If a simple protein detection scheme is the target, the available selection of HRP (Horseradish Peroxidase) products will complement any experimental design. Enzymes are synthesized in various quantities, so researchers have the option of 100µg or 100g of Trypsin, for example. Catalyze your research goals with the products in this enzyme collection.
Lack of consistent and rigorous QC is a major cause for concern
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Novel reagents and instruments are expanding capabilities
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Speed and enhanced PCR optimization functions like the 2D-Gradient make the Mastercycler X50 the ideal PCR cycler for advanced research in molecular biology. The excellent block temperature regulation gives rise to the next stage of PCR ...
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The MACSQuant® Tyto® Cell Sorter brings a gentler, safer, and sterile flow sorting technology to your lab. With the microchip technology at its heart, cells will no longer be exposed to electrical charge, decompression, and fluidic systems....
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I used Thermo Scientific RNase A to degrade unprotected mRNA during a gel retention assay to evaluate the protective effect of lipid nanoparticles (LNPs). Using 1 µL RNase A provided efficient RNA digestion while allowing clear discrimination between...
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I work extensively with cell wall hydrolases (enzymes) of bacteria. To check the enzymatic activity, I have to solubilize the intact cell wall of bacteria using mutanolysin. To examine the specific site of action on the cell wall, the resultant ...
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