
Modern molecular biology has been made possible thanks to the use of DNA modifying enzymes, which function to cleave, edit, unfold, join, or remove, segments of nucleic acids. Enabling a myriad of molecular applications, including cloning, mutagenesis, and DNA and RNA purification, these molecular tools encompass a variety of enzyme types. Among these, are DNA and RNA ligases, which connect two separate molecules of DNA and RNA, respectively. Various recombinases enable site-specific incorporation of sequences to form recombinant DNA constructs. Polynucleotide kinases can add phosphate groups or label DNA and RNA. Nucleases, including DNases and RNases, cut or degrade nucleic acids for applications such as purification or assays. Catalogued here are DNA or RNA modifying enzymes from several suppliers.
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A number of tools and techniques are needed to assess CQAs
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I used Thermo Scientific RNase A to degrade unprotected mRNA during a gel retention assay to evaluate the protective effect of lipid nanoparticles (LNPs). Using 1 µL RNase A provided efficient RNA digestion while allowing clear discrimination between...
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I work extensively with cell wall hydrolases (enzymes) of bacteria. To check the enzymatic activity, I have to solubilize the intact cell wall of bacteria using mutanolysin. To examine the specific site of action on the cell wall, the resultant ...
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