Endonucleases

Endonucleases cleave DNA or RNA chains internally from the phosphodiester bonds connecting the nucleotides. Representing a broad class of nucleases, they are involved in many biological functions, such as DNA repair, homologous recombination, and viral integration. Commercially produced Endonucleases are available for specialized applications in DNA manipulation. For instance, T7 Endonuclease I is used in recognition of short base deletions and insertions. AP Endonuclease creates single strand DNA breaks for DNA nicking. Benzonase , an engineered endonuclease, fully digests DNA and RNA down to 2 to 5 bases in length. For restriction endonucleases, see the “Restriction Enzyme” section in the enzyme search tool.