In 1976, Chien and colleagues first isolated Taq Polymerase from Thermus aquaticus, a thermophilic bacterium found in a hot spring in Yellowstone National Park. This polymerase was found to synthesize DNA at an optimal temperature of 75-80 °C and can survive temperatures up to 97 °C. Interestingly, while the authors were mainly interested in how life proliferates at extreme temperatures, they did not overlook the possibility of harnessing the enzyme for molecular biology. Sure enough, after Mullis invented the polymerase chain reaction in 1983, Taq was soon implemented as the active polymerizing enzyme by Saiki and others in 1988 giving birth to modern molecular biology. As if to follow Taq’s example, other thermostable polymerases eventually became prominent. Pfu Polymerase and Pfx Polymerase found uses in DNA amplifications requiring higher fidelity or accuracy. Tth polymerase with its intrinsic reverse transcriptase activity became ideal for reverse transcription and RT-PCR.
Modern systems are broadening the tech’s applications.
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Tips for deciding whether or not to use hybridization capture
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The MilliSentials™ Lab Labeling System provides a complete laboratory labeling solution with laboratory grade labels, a compact WIFI capable printer, and custom-designed laboratory labeling software. Features:
Laboratory grade labels
Labels can ...
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The QX Continuum Droplet Digital PCR (ddPCR) System combines the hallmark performance of ddPCR technology with the user-friendly experience of qPCR. It provides cost-effective absolute quantification of up to four targets from a single sample well, ...
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This enzyme was found to be better than any other commercially available TAQ polymerase. It works well for 200bp product amplification. Also there is no need for hot start.
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PCR is now a standard practice for all labs which are into molecular based examination. To get reproducible results, a lab should use well-standardized products which are sensitive and...
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