Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(561) The molecular hallmarks of primary and secondary vitreoretinal lymphomaBlood AdvancesMarch 8, 2022Irina Bonzheim et al.remaining vitrectomy material as previously described18 or using the Maxwell RSC DNA FFPE Kit and the Maxwell RSC Instrument (Promega, Madison, WI) according to the manufacturer’s instructions. PCR for the detection of IGH gene

(562) Molecular Characterization of Salmonella Detected along the Broiler Production Chain in Trinidad and TobagoMicroorganismsMarch 6, 2022Anisa Sarah Khan, Rian Ewald Pierneef, Narjol Gonzalez-Escalona, Meghan Maguire, Cong Li, Gregory H. Tyson, Sherry Ayers, Karla Georges, Woubit Abebe, Abiodun Adewale AdesiyunDNA Extraction and Sequencing DNA was extracted using the Maxwell RSC cultured cells DNA kit with a Maxwell RSC instrument (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocols for Gram-negative bacteria

(563) Sequencing of BRCA1/2-alterations using NGS-based technology: annotation as a challengeOncotargetMarch 3, 2022Silvana Ebner, Ria Winkelmann, Saskia Martin, Jens KÃ¶llermann, Peter J. Wild, Melanie Demesfor all three workflows. The purification of DNA from the FFPE tissue samples was performed using the Maxwell® RSC Instrument (Promega Corporation) with the Maxwell® RSC FFPE Plus DNA Kit (Promega Corporation). Therefore, the

(564) CAF-Associated Paracrine Signaling Worsens Outcome and Potentially Contributes to Chemoresistance in Epithelial Ovarian CancerFrontiers in OncologyMarch 3, 2022Michael Wessolly, Elena Mairinger, Sabrina Borchert, Agnes Bankfalvi, Pawel Mach, Kurt Werner Schmid, Rainer Kimmig, Paul Buderath, Fabian Dominik Mairingercommenced. 2.2.2 RNA Isolation RNA was isolated in a semi-automatic workflow with the help of the Maxwell® RSC Instrument (Promega, Wisconsin, USA) using a Maxwell® RSC RNA FFPE kit (AS1440, Promega, Wisconsin, USA). The process

(565) Spatial and temporal distribution of SARS-CoV-2 diversity circulating in wastewaterWater ResearchMarch 1, 2022Alba PÃ©rez-CataluÃ±a, Ãlvaro Chiner-Oms, Enric Cuevas-Ferrando, Azahara DÃaz-Reolid, Irene FalcÃ³, Walter Randazzo, InÃ©s GirÃ³n-GuzmÃ¡n, Ana Allende, MarÃa A. Bracho, IÃ±aki Comas, et al.transferred to the loading cartridge and 300 μL of lysis buffer added. The cartridge was then loaded in the Maxwell® RSC Instrument (Promega) using the “Maxwell RSC Viral total Nucleic Acid” running program for the nucleic acid extraction.

(566) Detection of SARS-CoV-2 genome on inanimate surfaces in COVID-19 intensive care units and emergency care cohortBrazilian Journal of MicrobiologyMarch 1, 2022Thabata Coaglio Lucas, Cristiane Rocha Fagundes Moura, Raquel Aparecida Monteiro, ValÃ©ria da Silva Baracho, Cintia Maria Rodrigues, Kamila Lorene Soares Rocha, Paulo Henrique da Cruz Ferreira, Thyago JosÃ© Silva, Etel Rocha-Vieira, Danilo Bretas de Oliveirato the manufacturer instructions, using the Maxwell® System: Maxwell® RSC simply RNA Cells Kit and Maxwell® RSC Instrument (Promega, Wisconsin, USA). Briefly, 200 μL of the sample was added to 250 μL of Lysis Buffer (Lysis Buffer

(567) Sequencing Depth Has a Stronger Effect than DNA Extraction on Soil Bacterial Richness DiscoveryBiomoleculesFebruary 25, 2022Concepcion Sanchez-Cid, Romie Tignat-Perrier, Laure Franqueville, Laurence DelauriÃ¨re, Trista Schagat, Timothy M. Vogelextraction method described by Griffiths et al. [29], as well as a new semiautomated protocol in which the Maxwell RSC Instrument (Promega, Madison, WI, USA) and a prototype version of the Maxwell RSC Fecal Microbiome DNA Kit (Promega)

(568) Proteasomal Processing Immune Escape Mechanisms in Platinum-Treated Advanced Bladder CancerGenes (Basel)February 25, 2022Michael Wessolly, Fabian D. Mairinger, Thomas Herold, Boris Hadaschik, Tibor Szarvas, Henning ReisAX4920 custom, Promega Maxwell, Fitchburg, MA, USA) on a Maxwell RSC device (Maxwell RSC Instrument AS4500, Promega Maxwell) according to the manufacturer’s manual (R29X) with minor adaptions. Proteinase K end

(569) Undetected αKlotho in serum is associated with the most aggressive phenotype of breast cancerMolecular and Clinical OncologyFebruary 25, 2022Bruna Cristina Borges, Pedro Augusto Do Amaral, Luiz Ricardo Soldi, Victor Luigi Costa Silva, Fernanda Carvalho De Souza, Felipe Andrés Cordeiro Da Luz, Rogério Agenor De Araújo, Marcelo José Barbosa SilvaRT Total RNA was first extracted using the Total SV RNA Isolation System kit (cat. no. Z3100) in a Maxwell® RSC Instrument (Promega Corporation), and complementary DNA (cDNA) was obtained from total RNA using the GoScript™

(570) Cyclosporine A Inhibits Viral Infection and Release as Well as Cytokine Production in Lung Cells by Three SARS-CoV-2 VariantsMicrobiology spectrumFebruary 23, 2022Fenizia C, Galbiati S, Vanetti C, Vago R, Clerici M, Tacchetti C, Daniele T.Maxwell RSC Viral Total Nucleic Acid purification kit was used to extract RNA from 250 μL of cell culture supernatants employing the Maxwell RSC Instrument (Promega, Madison, WI, USA). The remaining supernatant was conveniently stored at −80°C for the TCID50

(571) FYCO1 Frameshift Deletion in Wirehaired Pointing Griffon Dogs with Juvenile CataractGenes (Basel)February 11, 2022Gabriela Rudd Garces, Matthias Christen, Robert Loechel, Vidhya Jagannathan, Tosso Leebisolation. Genomic DNA was isolated from 500 µL of EDTA blood with the Maxwell RSC Whole-Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.3. Whole-Genome Sequencing of an Affected Dog An Illumina TruSeq

(572) Quantitative and Functional Assessment of the Influence of Routinely Used Cryopreservation Media on Mononuclear Leukocytes for Medical ResearchInternational Journal of Molecular SciencesFebruary 7, 2022Patrick Haider, Timothy Hoberstorfer, Manuel Salzmann, Michael B. Fischer, Walter S. Speidl, Johann Wojta, Philipp J. HohensinnerGene Expression Analysis RNA from MNC lysates was prepared using Maxwell RSC simplyRNA Tissue kits on a Maxwell RSC Instrument (both Promega, Madison, WI, USA). To create cDNA from the extracted RNA, 10 μL RNA was added to 10 μL

(573) ABHD5 frameshift deletion in Golden Retrievers with ichthyosisG3 Genes|Genomes|GeneticsFebruary 4, 2022Sarah Kiener, Dominique J Wiener, Kaitlin Hopke, Alison B Diesel, Vidhya Jagannathan, Elizabeth A Mauldin, Margret L Casal, Tosso Leebextraction Genomic DNA was extracted from EDTA blood using the Maxwell® RSC Whole Blood DNA with the Maxwell® RSC instrument (Promega, Dübendorf, Switzerland). Linkage analysis and homozygosity mapping Genotype data for 44

(574) DNA damage response at telomeres boosts the transcription of SARS‐CoV‐2 receptor ACE2 during agingEMBO reportsFebruary 3, 2022Sara Sepe et al.ratios. RNA extraction from cultured cells For MEFs, HeLa, and BJ cells, total RNA was extracted using Maxwell® RSC Instrument, with Maxwell® RSC simplyRNA Tissue Kit (AS134, Promega), following manufacturer’s instructions. NanoVueTM

(575) The Challenge of Long-Term Cultivation of Human Precision-Cut Lung SlicesThe American Journal of PathologyFebruary 1, 2022Eike B. PreuÃŸ, Stephanie Schubert, Christopher Werlein, Helge Stark, Peter Braubach, Anne HÃ¶fer, Edith K.J. Plucinski, Harshit R. Shah, Robert Geffers, Katherina Sewald, et al.RNASeq RNA was isolated using the Maxwell RSC simplyRNA Cells Kit (Promega, Madison, WI) with the Maxwell RSC Instrument as described in the instruction manual. PCLS were homogenized in 200 μL of chilled 1-thioglycerol/homogenization

(576) Performance of RT-qPCR detection of SARS-CoV-2 in unextracted nasopharyngeal samples using the Seegene AllplexTM 2019-nCoV protocolJournal of Virological MethodsFebruary 1, 2022Flavia R.O. Barros, Deborah C.A. Leite, Larissa J. GuimarÃ£es, Juliana M.H. Lopes, Marina W. Vasconcelos, Luciane X. Ferreira, Sandrieli GonÃ§alves, Victor G.F. Pastre, Gabriela Pereira, Alex B. Trentin, et al.thawed samples were incubated at 56 °C for 10 min with 330 μL of lysis solution and transferred to the Maxwell® RSC Instrument cartridges for RNA extraction according to manual instructions (Maxwell® 16 Viral Total Nucleic Acid Purification Kit

(577) Role of TGFβ1 and WNT6 in FGF2 and BMP4-driven endothelial differentiation of murine embryonic stem cellsAngiogenesisFebruary 1, 2022Gualandris A, Noghero A, Cora'' D, Astanina E, Arese M, Bussolino F.[25]. Real-time PCR Total RNA was extracted with Maxwell RSC miRNA Tissue Kit and processed with Maxwell RSC Instrument (Promega). RNAs were quantified with DeNovix spectrophotometer (DeNovix, Wilmington) and reverse transcribed

(578) Downregulation of lncRNA PVT1 inhibits proliferation and migration of mesothelioma cells by targeting FOXM1Oncology reportsFebruary 1, 2022Fujii Y, Amatya VJ, Kushitani K, Suzuki R, Kai Y, Kambara T, Takeshima Y.supplied with 5% CO2 for 72 h. RNA was extracted from the cells using Maxwell® RSC simplyRNA Cells Kit and Maxwell® RSC Instrument (both from Promega Corporation) according to the manufacturer's protocols. The extracted RNA was reverse-transcribed

(579) Liraglutide + PYY3-36 Combination Therapy Mimics Effects of Roux-en-Y Bypass on Early NAFLD Whilst Lacking-Behind in Metabolic ImprovementsJournal of Clinical MedicineJanuary 30, 2022Valentin Metzner, Gloria Herzog, Tobias Heckel, Thorsten Bischler, Julia Hasinger, Christoph Otto, Martin Fassnacht, Andreas Geier, Florian Seyfried, Ulrich DischingerMaxwell® RSC simplyRNA Tissue Kit (AS1340; Promega, Fitchburg, WI, USA) and the Maxwell® RSC Instrument (AS4500; Promega). Prior to extraction, tissues were homogenized using QIAGEN Tissue Lyser II (85300; QIAGEN,

(580) Phenotyping and Quantitative Trait Locus Analysis for the Limited Transpiration Trait in an Upper-Mid South Soybean Recombinant Inbred Line Population (“Jackson” × “KS4895”): High Throughput Aquaporin Inhibitor ScreeningFrontiers in Plant ScienceJanuary 20, 2022Sayantan Sarkar, Avat Shekoofa, Angela McClure, Jason D. Gillmanlines “KS4895” and “Jackson” using a Promega AS1600 kit (Promega, Madison, WI, United States) using a Maxwell RSC instrument (Promega). Two genotypes did not germinate and the leaf tissue could not be collected. Genotypes for

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