Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(501) Detection of BRCA1/2 pathogenic variants in patients with breast and/or ovarian cancer and their families. Analysis of 3,458 cases from Lower Silesia (Poland) according to the diagnostic algorithm of the National Cancer Control ProgrammeFrontiers in GeneticsSeptember 12, 2022Anna Doraczynska-Kowalik et al.Maxwell RSC Blood DNA Kit (Promega Corporation) according to the manufacturer’s protocol on a Maxwell RSC instrument (AS4500, Promega Corporation). After extraction, the DNA samples were quantified using the fluorometric method,

(502) Integrated Antitumor Activities of Cellular Immunotherapy with CIK Lymphocytes and Interferons against KIT/PDGFRA Wild Type GISTInternational journal of molecular sciencesSeptember 8, 2022Fiorino E, Merlini A, D'Ambrosio L, Cerviere I, Berrino E, Marchiò C, Giraudo L, Basiricò M, Massa A, Donini C, et al.Analysis Total RNA automatic extraction of wtGISTc was performed using the Maxwell RSC miRNA Tissue KIT and Maxwell RSC Instrument (Promega, Milan, Italy), according to the manufacturer’s instructions. The RNA was reverse-transcribed

(503) Prospective evaluation of the role of imaging techniques and TMPRSS2:ERG mutation for the diagnosis of clinically significant prostate cancerFrontiers in OncologySeptember 6, 2022Massimo Lazzeri, Vittorio Fasulo, Giovanni Lughezzani, Alessio Benetti, Giulia SoldÃ , Rosanna Asselta, Ilaria De Simone, Marco Paciotti, Pier Paolo Avolio, Roberto Contieri, et al.withdrawal. RNA was extracted using the Maxwell RSC miRNA Tissue Kit (Promega, Madison, WI, USA) on a Maxwell RSC instrument (Promega). Random primers (Promega) and the Superscript-IV Reverse Transcriptase (Thermo Fisher Scientific,

(504) CYP2C8 *3 and *4 define CYP2C8 phenotype: An approach with the substrate cinitaprideClinical and Translational ScienceSeptember 6, 2022Diana MarÃa CampodÃ³nico, Pablo Zubiaur, Paula Soriaâ€Chacartegui, Ana CasajÃºs, Gonzalo Villapalosâ€GarcÃa, Marcos Navaresâ€GÃ³mez, AntÃa GÃ³mezâ€FernÃ¡ndez, RaÃºl Parraâ€GarcÃ©s, Gina MejÃaâ€Abril, Manuel RomÃ¡n, et al.phenotyping DNA was extracted from 500 μl of thawed peripheral blood stored in EDTA‐K2 tubes in a Maxwell® RSC Instrument (Promega Biotech Ibérica S.L.). Genotyping was performed by real‐time quantitative polymerase chain reaction

(505) Interleukin‐4 receptor alpha signaling regulates monocyte homeostasisFASEB JSeptember 5, 2022Patrick Haider, Julia B. Kral‐Pointner, Manuel Salzmann, Florian Moik, Sonja Bleichert, Waltraud C. Schrottmaier, Christoph Kaun, Mira Brekalo, Michael B. Fischer, Walter S. Speidl, et al.Quantitative PCR RNA from cell culture lysates was prepared using Maxwell RSC simplyRNA Tissue kits on a Maxwell RSC Instrument (both Promega, Madison, WI, USA). cDNA was created using a 1:1 Oligo(dT)/Random Primer Reverse Transcriptase

(506) Gut dysbiosis in cutaneous Tâ€cell lymphoma is characterized by shifts in relative abundances of specific bacterial taxa and decreased diversity in more advanced diseaseJournal of the European Academy of Dermatology and VenereologySeptember 1, 2022M.J. Hooper, T.M. LeWitt, Y. Pang, F.L. Veon, G.E. Chlipala, L. Feferman, S.J. Green, D. Sweeney, K.T. Bagnowski, M.B. Burns, et al.Genomic DNA was extracted with a Maxwell® RSC Fecal Microbiome DNA Kit (Promega; Madison, WI, USA) on a Maxwell® RSC Instrument, following the manufacturer's protocol. 16S rRNA amplification and sequencing Genomic DNA was prepared

(507) Development of a prediction protocol for the screening of metabolic associated fatty liver disease in children with overweight or obesityPediatric ObesitySeptember 1, 2022Maddi Oses, Cristina Cadenasâ€Sanchez, MarÃa Medrano, Arkaitz Galbete, Emiliano Mirandaâ€Ferrua, Jonatan R. Ruiz, Felix SÃ¡nchezâ€Valverde, Francisco B. Ortega, Rafael Cabeza, Arantxa Villanueva, et al.calculated. 32 Genomic DNA was extracted from white blood cells using Maxwell® RSC Blood DNA Kit and Maxwell® RSC Instrument (Promega) equipment. The genotyping was done by an Illumina system (Illumina, Inc, San Diego, CA, USA)

(508) Acute lymphoblastic leukemia necessitates GSH-dependent ferroptosis defenses to overcome FSP1-epigenetic silencingRedox biologySeptember 1, 2022Pontel LB, Bueno-Costa A, Morellato AE, Carvalho Santos J, Roué G, Esteller M.cell pellets using the SimplyRNA kit (Promega #AS1340) in the automated Maxwell RSC device (Promega, #AS4500). 2 μg of total RNA were converted to cDNA using the RevertAid First Strand cDNA synthesis kit (Thermofisher Scientific

(509) SARS‐CoV‐2 surveillance in Norway rats ( Rattus norvegicus ) from Antwerp sewer system, BelgiumTransboundary and Emerging DiseasesSeptember 1, 2022Valeria Carolina Colombo et al.concentrate with the automated Maxwell PureFood GMO and the Authentication RNA extraction kit in the Maxwell® RSC Instrument (Promega). Reverse transcription and amplification of the N1, N2 and E genes was performed by qPCR in

(510) Gut Microbiome and Mycobiome Alterations in an In Vivo Model of Alzheimerâ€™s DiseaseGenes (Basel)August 31, 2022Valeria Dâ€™Argenio, Iolanda Veneruso, Chunmei Gong, Valentina Cecarini, Laura Bonfili, Anna Maria Eleuteriprocessing. Genomic DNA was extracted from all stool samples by using the RSC Blood DNA kit and the Maxwell RSC instrument (both from Promega, Madison, WI, USA), as previously reported [19]. Specifically, 100 mg of each stool

(511) DNA damage response and repair genes in advanced bone and soft tissue sarcomas: An 8-gene signature as a candidate predictive biomarker of response to trabectedin and olaparib combinationFrontiers in OncologyAugust 30, 2022Alessandra Merlini, Maria Laura Centomo, Giulio Ferrero, Giulia Chiabotto, Umberto Miglio, Enrico Berrino, Giorgia Giordano, Silvia Brusco, Alberto Pisacane, Elena Maldi, et al.extracted from FFPE samples with Maxwell® RSC FFPE Kit (Promega Corporation, Madison, WI, USA) and Maxwell® RSC Instrument. RNA purity, concentration and fragmentation were determined using DeNovix DS-11+ Spectrophotometer

(512) Colorectal Adenocarcinomas Harboring ALK Fusion Genes: A Clinicopathologic and Molecular Genetic Study of 12 Cases and Review of the LiteratureAmerican Journal of Surgical PathologyAugust 29, 2022Jerzy Lasota et al.next-generation sequencing were previously reported.24 Briefly, nucleic acids were recovered from FFPE tumor samples using a Maxwell® RSC instrument (Promega, Madison, WI). Target-specific RNA libraries for next-generation sequencing (NGS) were constructed

(513) EHBP1L1 Frameshift Deletion in English Springer Spaniel Dogs with Dyserythropoietic Anemia and Myopathy Syndrome (DAMS) or Neonatal LossesGenes (Basel)August 26, 2022Sarah Ã˜stergÃ¥rd Jensen, Matthias Christen, Veronica Rondahl, Christopher T. Holland, Vidhya Jagannathan, Tosso Leeb, Urs Gigeraccording to the respective standard methods for EDTA blood, native tissue, and FFPE tissue using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). DNA from five affected and 19 unaffected animals was genotyped on

(514) A unifying hypothesis for PNMZL and PTFL: morphological variants with a common molecular profileBlood AdvancesAugust 23, 2022Julia Salmeron-Villalobos, Caoimhe Egan, Vanessa Borgmann, Inga MÃ¼ller, Blanca Gonzalez-Farre, Joan Enric Ramis-Zaldivar, Dominik Nann, Olga BalaguÃ©, MÃ³nica LÃ³pez-Guerra, Dolors Colomer, et al.Maxwell 16 FFPE Tissue LEV DNA Purification Kit or Maxwell RSC DNA FFPE Kit and the Maxwell 16 Instrument or the Maxwell RSC Instrument (Promega, Madison, WI) according to the manufacturer’s protocol. Polymerase chain reaction amplifications

(515) Sequencing identifies multiple early introductions of SARS-CoV-2 to the New York City RegionmedRxivAugust 18, 2022Matthew T. Maurano et al.using two platforms for parallel sample processing: Using the Maxwell RSC instrument (Promega, cat. AS4500), total RNA was extracted from 300 μL of viral transport medium with the buccal swab DNA kit (Promega,

(516) ABO , secretor, and Lewis carbohydrate histoâ€blood groups are associated with autoimmune neutropenia of early childhood in Danish patientsTransfusionAugust 1, 2022Kirstine KlÃ¸veâ€Mogensen, Rudi Steffensen, Tania Nicole Masmas, Andreas GlenthÃ¸j, Thure Mors Haunstrup, Paul Ratcliffe, Petter HÃ¶glund, Henrik Hasle, Kaspar RenÃ© Nielsenwhole blood or buccal swap using the Maxwell 16 Blood DNA Kit or the Maxwell RSC Buccal Swab Kit on the Maxwell RSC instrument (Promega, US). 2.3 ABO, FUT2, FUT3 and RhD genotyping Three SNPs in ABO (rs8176719 (G216del), rs7853989

(517) Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancerCancer MedicineAugust 1, 2022Takaomi Hayashi, Yoichiro Yoshida, Teppei Yamada, Keita Tanaka, Hideki Shimaoka, Ryuji Kajitani, Taro Munechika, Hideki Nagano, Yoshiko Matsumoto, Akira Komono, et al.was extracted from 1.0 ml plasma using the Maxwell® RSC cfDNA plasma kit (Promega Corporation) and Maxwell® RSC Instrument (Promega Corporation) in accordance with the manufacturer's protocol, as described previously. 21 2.4

(518) Routine Immunohistochemical Analysis of Mismatch Repair Proteins in Colorectal Cancerâ€”A Prospective AnalysisCancers (Basel)July 31, 2022Joana Lemos Garcia, Isadora Rosa, Sofia Saraiva, InÃªs Marques, Ricardo Fonseca, Pedro Lage, InÃªs Francisco, PatrÃcia Silva, Bruno Filipe, Cristina Albuquerque, et al.Maxwell® RSC DNA FFPE Kit (Promega, Madison, WI, USA) was used to isolate DNA from FFPE samples in the Maxwell® RSC Instrument (Promega). Each tumor and paired normal DNA were amplified by PCR for each of the microsatellite markers,

(519) Circulating miR-1246 and miR-485-3p as Promising Biomarkers of Clinical Response and Outcome in Melanoma Patients Treated with Targeted TherapyCancers (Basel)July 29, 2022Lauretta Levati et al.Maxwell® RSC miRNA Tissue Kit AS1460 (Promega, Madison, WI, USA) in combination with the Maxwell® RSC Instrument AS4500 (Promega). Plasma volume was mixed with 200 μL chilled 1-thioglycerol/homogenization solution and 200

(520) Anandamide Alters Barrier Integrity of Bovine Vascular Endothelial Cells during Endotoxin ChallengeAntioxidants (Basel)July 27, 2022Carsten C. F. Walker, Lorraine M. Sordillo, G. Andres Contreras−20 °C for no longer than one month before processing. Extraction of RNA occurred utilizing a Promega Maxwell RSC Instrument following the manufacturer’s protocol. RNA quantity and quality were evaluated using a NanoDrop ND-1000

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