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Automated DNA/RNA Purification Sample Preparation Workstations
Maxwell RSC Instrument
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Maxwell RSC Instrument
Maxwell RSC Instrument
Maxwell RSC Instrument from Promega
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Citations:
(849)
Figures:
(1)
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Maxwell RSC Instrument
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Description
The Maxwell RSC Instrument is a platform for automated extraction of nucleic acid from a range of sample types. Extraction methods use sample lysis and binding to paramagnetic particles followed by washing steps and elution.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC Instrument
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS4500
Quantity
EA
Processing Time
30 to 60 min (depending on sample type and method)
Capacity
Up to 16
Dimensions
(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV Lamp
Avg. lifetime approximately 6,000 hrs
Molecular Weight
~85kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (849)
(601)
Immunological Characterization of HIV and SARS-CoV-2 Coinfected Young Individuals
Cells
November 16, 2021
Claudia Vanetti et al.
Maxwell® RSC Viral Total Nucleic Acid Purification Kit
(
Promega
, Fitchburg, WI, USA) was used to extract
SARS-CoV-2
RNA using the
Maxwell® RSC Instrument
(
Promega
, Fitchburg, WI, USA). Viral RNA was quantified as previously described [9]. Briefly, a single-step
(602)
A direct capture method for purification and detection of viral nucleic acid enables epidemiological surveillance of SARS-CoV-2
Science of The Total Environment
November 15, 2021
Subhanjan Mondal, Nathan Feirer, Michael Brockman, Melanie A. Preston, Sarah J. Teter, Dongping Ma, Said A. Goueli, Sameer Moorji, Brigitta Saul, James J. Cali
nucleic acid purification
was performed using
Maxwell® RSC PureFood GMO and Authentication Kit
(
Promega
Corp.) using the
Maxwell® RSC Instrument
(
Promega
Corp.) according to a modified version of manufacturer's protocol. Briefly, to 200 μL of wastewater
(603)
Glucocorticoids Induce Partial Remission of Focal Segmental Glomerulosclerosis but Not Interstitial Nephritis in COVID-19 Acute Kidney Injury in an APOL1 Low-Risk Genotype White Patient
American Journal of Case Reports
November 2, 2021
Piotr J. Nowak, Joanna Forycka, Natalia Cegielska, Karolina Harendarz, Małgorzata Wągrowska-Danilewicz, Marian Danilewicz, Tomasz Płoszaj, Maciej Borowiec, Rafał Wlazeł, Michał Nowicki
the gene sequence matched the hg19 reference sequence. DNA was isolated from peripheral blood using a
Maxwell® RSC instrument
(
Promega
). Fragments of APOL1 gene (chr22: 36661747-36662448; 702bp) were amplified using forward GCCAGAGCCAATCTTCAGTC
(604)
Glucocorticoids Induce Partial Remission of Focal Segmental Glomerulosclerosis but Not Interstitial Nephritis in COVID-19 Acute Kidney Injury in an APOL1 Low-Risk Genotype White Patient
Am J Case Rep
November 2, 2021
Piotr J. Nowak, Joanna Forycka, Natalia Cegielska, Karolina Harendarz, Małgorzata Wągrowska-Danilewicz, Marian Danilewicz, Tomasz Płoszaj, Maciej Borowiec, Rafał Wlazeł, Michał Nowicki
the gene sequence matched the hg19 reference sequence. DNA was isolated from peripheral blood using a
Maxwell® RSC instrument
(
Promega
). Fragments of APOL1 gene (chr22: 36661747-36662448; 702bp) were amplified using forward GCCAGAGCCAATCTTCAGTC
(605)
Liquid biopsy with droplet digital PCR targeted to specific mutations in plasma cell-free tumor DNA can detect ovarian cancer recurrence earlier than CA125
Gynecologic Oncology Reports
November 1, 2021
Takamichi Minato, Shin Ito, Bin Li, Haruna Fujimori, Mai Mochizuki, Kazunori Yamaguchi, Keiichi Tamai, Muneaki Shimada, Hideki Tokunaga, Shogo Shigeta,
et al.
DNA was extracted with the
Maxwell RSC DNA FFPE Kit
- PKK, Custom (AX2500;
Promega
, Madison, WI) and
Maxwell RSC Instrument
(
Promega
) according to the manufacturer's instructions. In total, 590 ng to 4,480 ng whole genomic DNA
(606)
Hepatoblastoma: glutamine depletion hinders cell viability in the embryonal subtype but high GLUL expression is associated with better overall survival
Journal of cancer research and clinical oncology
November 1, 2021
Schmidt A, Armento A, Bussolati O, Chiu M, Ellerkamp V, Scharpf MO, Sander P, Schmid E, Warmann SW, Fuchs J.
was extracted from macro-dissected 5 µm paraffin sections using the
Maxwell® RSC DNA FFPE Kit
and the
Maxwell® RSC Instrument
(
Promega
, Madison, WI, USA) according to the manufacturer’s instructions. CTNNB1 exon 3 was investigated
(607)
SARS-CoV-2 Exposed Mesenchymal Stromal Cell from Congenital Pulmonary Airway Malformations: Transcriptomic Analysis and the Expression of Immunomodulatory Genes
International Journal of Molecular Sciences
October 30, 2021
Andrea Valeri et al.
investigate the cytopathic effect.
SARS-CoV-2
RNA was extracted from 24, 48, and 72 hpi supernatant using the
Maxwell® RSC Instrument
with
Maxwell® RSC Viral Total Nucleic Acid Purification Kit
(
Promega
, Fitchburg, WI, USA). Viral RNA
(608)
EphA2 Expression in Bone Sarcomas: Bioinformatic Analyses and Preclinical Characterization in Patient-Derived Models of Osteosarcoma, Ewing's Sarcoma and Chondrosarcoma
Cells
October 26, 2021
Giordano G, Merlini A, Ferrero G, Mesiano G, Fiorino E, Brusco S, Centomo ML, Leuci V, D'Ambrosio L, Aglietta M,
et al
.
tissue and normal counterparts upon homogenization by using
Promega
a
Maxwell RSC miRNA tissue Kit
and a
Maxwell RSC Instrument
(
Promega
Italia, MI, Italy). RNA quality and concentration were checked by
DS 11+ Spectrophotometer
(609)
A multiplexed ion-exchange membrane-based miRNA (MIX·miR) detection platform for rapid diagnosis of myocardial infarction
Lab on a Chip
October 12, 2021
Xiang Ren, Bradley W. Ellis, George Ronan, Stuart Ryan Blood, Cameron DeShetler, Satyajyoti Senapati, Keith L. March, Eileen Handberg, David Anderson, Carl Pepine,
et al.
aliquoted and stored at −20°C.
miRNA
isolation for
RT-qPCR
miRNA
was isolated from
human
plasma using the
Maxwell RSC instrument
(
Promega
, Wisconsin, USA) and
miRNA
Plasma or Serum miRNA isolation kit (Promega) with lysing solution
(610)
Genetic evaluation of small ruminant lentivirus susceptibility in Valais blacknose sheep
Animal Genetics
October 1, 2021
Anna Letko, Charis Bützberger, Nathalie Hirter, Julia M. Paris, Carlos Abril, Cord Drögemüller
Reinach, Switzerland). Therefore, genomic DNA was extracted from
EDTA
‐stabilized blood samples using the
Maxwell RSC instrument
(
Promega
, Dübendorf, Switzerland). Interestingly, no homozygous wild type genotypes were observed for
(611)
An alternative approach for bioanalytical assay optimization for wastewater-based epidemiology of SARS-CoV-2
Science of The Total Environment
October 1, 2021
Tim Boogaerts, Lotte Jacobs, Naomi De Roeck, Siel Van den Bogaert, Bert Aertgeerts, Lies Lahousse, Alexander L.N. van Nuijs, Peter Delputte
Powermicrobiome kit were obtained from QIAGEN (Hilden, DE). Automated RNA extraction was done with the
Maxwell® RSC Instrument
with the PureFood GMO and Authentication kit, both from
Promega
(Madison, US). Initially, a selection
(612)
The long non-coding RNA HOTAIRM1 promotes tumor aggressiveness and radiotherapy resistance in glioblastoma
Cell Death & Disease
October 1, 2021
Ulvi Ahmadov et al.
quantitative PCR
Total RNA extraction was performed by using TRIzol (#
15596026
,
Thermo Fischer Scientific
) or by using a
Maxwell RSC instrument
(#
AS1340
, RSC simplyRNA Tissue,
Promega
, Madison, WI, USA). Reverse transcription of total RNA (1 µg)
(613)
Variants in COMT, CYP3A5, CYP2B6, and ABCG2 Alter Quetiapine Pharmacokinetics
Pharmaceutics
September 28, 2021
Pablo Zubiaur, Paula Fernández-Campos, Marcos Navares-Gómez, Paula Soria-Chacartegui, Gonzalo Villapalos-GarcÃa, Manuel Román, Gina MejÃa-Abril, Dolores Ochoa, Francisco Abad-Santos
study. 2.3.
Genotyping
, Haplotyping, and Phenotyping DNA was extracted from peripheral blood in a
Maxwell® RSC Instrument
(
Promega
Biotech Ibérica S.L., Alcobendas, Madrid, Spain).
Genotyping
was performed by real-time quantitative
(614)
Molecular phylogenetic analysis of bovine papular stomatitis viruses detected in Saga, Japan
Journal of Veterinary Medical Science
September 27, 2021
Shinji HIRANO, Yasufumi SOGA, Yuri KUNO, Satomi DOAI, Shinya TOKUTAKE, Tomoyuki SHIBAHARA, Tadahiro INDO
homogenates using a
Maxwell RSC Viral Total Nucleic Acid Purification Kit
(
Promega
, Madison, WI, USA) with a
Maxwell RSC Instrument
(
Promega
), according to the manufacturer’s instructions.
PCR
was performed with AmpliTaq Gold (Applied
(615)
MIA3 Splice Defect in Cane Corso Dogs with Dental-Skeletal-Retinal Anomaly (DSRA)
Genes (Basel)
September 25, 2021
Matthias Christen, Henriëtte Booij-Vrieling, Jelena Oksa-Minalto, Cynthia de Vries, Alexandra Kehl, Vidhya Jagannathan, Tosso Leeb
Genotyping
Genomic DNA was isolated from
EDTA
blood samples with the
Maxwell RSC Whole Blood DNA Kit
using a
Maxwell RSC instrument
(
Promega
, Dübendorf, Switzerland). DNA from 12 affected and 8 unaffected animals was genotyped on illumina_HD
(616)
PRKG2 Splice Site Variant in Dogo Argentino Dogs with Disproportionate Dwarfism
Genes (Basel)
September 24, 2021
Gabriela Rudd Garces, Maria Elena Turba, Myriam Muracchini, Alessia Diana, Vidhya Jagannathan, Fabio Gentilini, Tosso Leeb
DNA Isolation Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a
Maxwell RSC instrument
(
Promega
, Dübendorf, Switzerland). 2.3. Linkage and Homozygosity Mapping The Dogo Argentino family,
(617)
MYO5A Frameshift Variant in a Miniature Dachshund with Coat Color Dilution and Neurological Defects Resembling Human Griscelli Syndrome Type 1
Genes (Basel)
September 23, 2021
Matthias Christen, Madeleine de le Roi, Vidhya Jagannathan, Kathrin Becker, Tosso Leeb
Extraction Genomic DNA was isolated from the EDTA blood with the Maxwell RSC Whole Blood Kit using a
Maxwell RSC instrument
(
Promega
, Dübendorf, Switzerland). 2.5.
Whole-Genome Sequencing
An Illumina TruSeq
PCR
-free DNA library
(618)
Analysis of Telomere Maintenance Related Genes Reveals NOP10 as a New Metastatic-Risk Marker in Pheochromocytoma/Paraganglioma
Cancers (Basel)
September 23, 2021
María Monteagudo et al.
samples using the Maxwell® RSC DNA
Formalin-fixed paraffin embedded
Kit (Promega, Madison, WI, USA) and a
Maxwell® RSC Instrument
(
Promega
). DNA from frozen tissue was extracted with DNeasy® Blood and Tissue Kit (Qiagen, Hilden, Germany),
(619)
Prolyl isomerase Pin1 plays an essential role in SARS-CoV-2 proliferation, indicating its possibility as a novel therapeutic target
Scientific reports
September 17, 2021
Yamamotoya T, Nakatsu Y, Kanna M, Hasei S, Ohata Y, Encinas J, Ito H, Okabe T, Asano T, Sakaguchi T.
Sendai, Japan) as a standard. Cellular RNA in the infected VeroE6/TMPRSS2 cells was prepared using the
Maxwell RSC instrument
(
Promega
Corp., Madison, WI) according to the manufacturer’s protocol.
RT-qPCR
for specific
amplification
(620)
PD-L1 Dependent Immunogenic Landscape in Hot Lung Adenocarcinomas Identified by Transcriptome Analysis
Cancers (Basel)
September 11, 2021
Jutta Kirfel et al.
mRNA isolation and quantification were performed using the
Maxwell RSC RNA FFPE Kit
together with the
Maxwell RSC instrument
(
Promega
, Fitchburg, WI, USA) and the Qubit fluorimeter (ThermoFisher, Waltham, MA, USA), respectively.
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Maxwell RSC Instrument from Promega
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