Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(601) Gene therapy with AR isoform 2 rescues spinal and bulbar muscular atrophy phenotype by modulating AR transcriptional activityScience advancesAugust 1, 2021Lim WF, Forouhan M, Roberts TC, Dabney J, Ellerington R, Speciale AA, Manzano R, Lieto M, Sangha G, Banerjee S, et al.was isolated from murine tissues or cell lines using Maxwell RSC simplyRNA tissue or cells kit and Maxwell RSC Instrument (Promega) according to the manufacturer’s protocol. One microgram of RNA was used for cDNA synthesis

(602) MiRNA Let-7a and Let-7d Are Induced by Globotriaosylceramide via NF-kB Activation in Fabry DiseaseGenes (Basel)July 30, 2021Nadine Maier, Constantin Gatterer, Patrick Haider, Manuel Salzmann, Christoph Kaun, Walter S. Speidl, Gere Sunder-Plassmann, Bruno K. Podesser, Johann Wojta, Senta Graf, et al.previously [16]. RNA from cell culture lysates was prepared using Maxwell RSC simplyRNA Tissue kits on a Maxwell RSC Instrument (both Promega, Madison, WI, USA). To create cDNA from the extracted RNA, 10 μL sample were added to

(603) SARS-CoV-2 Infected Pediatric Cerebral Cortical Neurons: Transcriptomic Analysis and Potential Role of Toll-like Receptors in PathogenesisInternational Journal of Molecular SciencesJuly 28, 2021Agnese Gugliandolo et al.effect. SARS-CoV-2 RNA was extracted from 1, 3 and 6 days post infection (dpi) supernatant using the Maxwell® RSC Instrument with Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Fitchburg, WI, USA). Viral RNA

(604) Gene Expression Analysis Provides New Insights into the Mechanism of Intramuscular Fat Formation in Japanese Black CattleGenes (Basel)July 21, 2021Shuji Ueda, Mana Hosoda, Ken-ichi Yoshino, Minoru Yamanoue, Yasuhito Shirai°C. Total RNA was purified using ~100 mg of fat tissue by the Maxwell RSC simply RNA tissue kit using a Maxwell RSC instrument (Promega K. K., Tokyo, Japan) according to manufacturer instructions. The concentration of the obtained

(605) Comparative genomic analysis of Escherichia coli isolates from cases of bovine clinical mastitis identifies nine specific pathotype marker genesMicrobial GenomicsJuly 6, 2021Dongyun Jung, Soyoun Park, Janina Ruffini, Forest Dussault, Simon Dufour, Jennifer RonholmDNA from isolates that did not produce high-quality DNA via this method was re-extracted using the Maxwell RSC instrument and the recommended Blood DNA kit (Promega), according to the manufacturer’s instructions. A DNA concentration

(606) CB ₁ R and iNOS are distinct players promoting pulmonary fibrosis in Hermansky–Pudlak syndromeClinical and Translational MedicineJuly 4, 2021Resat Cinar et al.frozen. RNA was collected using Maxwell® RSC simplyRNA Cells Kit (AS1390) on a Maxwell® RSC Instrument (AS4500, Promega). RNA quality and quantity were measured using QuantiFluor® RNA System (E3310) on a Quantus™ Fluorometer

(607) Multiple Potential Plant Growth Promotion Activities of Endemic Streptomyces spp. from Moroccan Sugar Beet Fields with Their Inhibitory Activities against Fusarium spp.MicroorganismsJuly 2, 2021Yassine Aallam, Bouchra El Maliki, Driss Dhiba, Sanaa Lemriss, Amal Souiri, Abdelmajid Haddioui, Mika Tarkka, Hanane Hamdaliof the suspension containing fragmented mycelium was used for automated extraction of DNA with the Maxwell® RSC Instrument (Promega, Madison, WI, USA) and the Maxwell RSC PureFood GMO and Authentication Kit (Promega) according

(608) Uncertainties in estimating SARS-CoV-2 prevalence by wastewater-based epidemiologyChemical Engineering JournalJuly 1, 2021Xuan Li, Shuxin Zhang, Jiahua Shi, Stephen P. Luby, Guangming Jiang(Promega),SARS-CoV-2,7.4 ± 7.9% −9.4 ± 14.7%,1.07–1.56 Precipitation,Aluminum hydroxide adsorption-precipitation,Maxwell® RSC Instrument,RT-qPCR,SARS-CoV-2,30.2 ± 17.7%,0.59,[98] Precipitation,Aluminum hydroxide adsorption-precipitation,Maxwell®

(609) Loss of coordinated expression between ribosomal and mitochondrial genes revealed by comprehensive characterization of a large family with a rare Mendelian disorderGenomicsJuly 1, 2021Brendan Panici, Hosei Nakajima, Colleen M. Carlston, Hakan Ozadam, Can Cenik, Elif Sarinay Cenikindicated in Carlston et al. 2017. RNA extraction and library preparation RNA was extracted using Maxwell RSC instrument (Promega) from 50 ul of whole blood specimens with a white cell count greater than 50 000/μl. For depletion

(610) Diagnostic Utility of a Custom 34-Gene Anchored Multiplex PCR-Based Next-Generation Sequencing Fusion Panel for the Diagnosis of Bone and Soft Tissue Neoplasms With Identification of Novel USP6 Fusion Partners in Aneurysmal Bone CystsArchives of Pathology & Laboratory MedicineJuly 1, 2021Josephine K. Dermawan et al.was performed by using the Maxwell RSC FFPE Kits (catalog Nos. AS1440 and AS1450, Promega) and protocol on the Maxwell RSC instrument. Total nucleic acid was quantified by using both the Qubit Ribonucleic acid (RNA) HS Assay Kit (catalog

(611) A global metagenomic map of urban microbiomes and antimicrobial resistanceCellJune 24, 2021David Danko, Daniela Bezdan, Evan E. Afshin, Sofia Ahsanuddin, Chandrima Bhattacharya, Daniel J. Butler, Kern Rei Chng, Daisy Donnellan, Jochen Hecht, Katelyn Jackson, et al.automation of sample processing for nucleic acid extraction using the Maxwell RSC Instrument (Promega, Cat.: AS4500) began in 2017 using the Maxwell RSC Buccal Swab Kit (Promega, Cat.: AS1640). DNA extraction from copan

(612) Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancerScientific ReportsJune 24, 2021Keita Tanaka, Yoichiro Yoshida, Teppei Yamada, Takaomi Hayashi, Hideki Shimaoka, Fumihiro Yoshimura, Ryuji Kajitani, Taro Munechika, Yoshiko Matsumoto, Hideki Nagano, et al.extracted from 1 ml plasma using the Maxwell® RSC cfDNA plasma kit (Promega Corporation, Madison, USA) and Maxwell® RSC Instrument (Promega Corporation, Madison, USA) in accordance with the manufacturer’s protocol, as described p

(613) Long-Term Grow-Out Affects Campylobacter jejuni Colonization Fitness in Coincidence With Altered Microbiota and Lipid Composition in the Cecum of Laying HensFrontiers in Veterinary ScienceJune 18, 2021Hiroshi Asakura, Tatsuya Nakayama, Shiori Yamamoto, Kazuki Izawa, Jun Kawase, Yasushi Torii, Satoshi Murakamiμl of lysis buffer (Promega). DNA extraction was then carried out using a Maxwell Blood DNA kit in a Maxwell RSC instrument (Promega). The concentration and quality of the extracted DNA were measured on a Tape Station 4150 system

(614) Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis rootsMolecular Systems BiologyJune 16, 2021Isabel BetegÃ³nâ€Putze, Josep Mercadal, Nadja Bosch, Ainoa Planasâ€Riverola, Mar MarquÃ¨sâ€Bueno, Josep Vilarrasaâ€Blasi, David Frigola, Rebecca C Burkart, Cristina MartÃnez, Ana Conesa, et al.assay RNA was extracted from root tip tissue with the Maxwell® RSC Plant RNA Kit (Promega) using the Maxwell® RSC instrument (Promega) according to the manufacturer's recommendations, and concentrations were checked using NanoDrop

(615) Genetic Characterization of Cancer of Unknown Primary Using Liquid Biopsy ApproachesFrontiers in Cell and Developmental BiologyJune 10, 2021Noemi Laprovitera et al.FoundationOne CDx (F1CDx) assay. ccfDNA was purified from 1 ml of plasma by Maxwell RSC instrument (Cat No: AS4500, Promega, Madison, WI, United States) using the Maxwell RSC ccfDNA Plasma Kit (Cat No: AS1480, Promega).

(616) Macrophagic Inflammatory Response Next to Dental Implants with Different Macro- and Micro-Structure: An In Vitro StudyApplied SciencesJune 8, 2021Maria Menini, Francesca Delucchi, Domenico Baldi, Francesco Pera, Francesco Bagnasco, Paolo Pesce(Promega, Madison, WI, USA) following the supplier’s instructions and the nucleic acid automated extractor Maxwell® RSC instrument (Promega). RNAs of Appl. Sci. 2021, 11, 5324 4 of 10 extracted samples at 4 h, eluted in H2O,

(617) SARS-CoV-2 Entry: At the Crossroads of CD147 and ACE2CellsJune 8, 2021Fenizia C, Galbiati S, Vanetti C, Vago R, Clerici M, Tacchetti C, Daniele T.Maxwell® RSC Viral Total Nucleic Acid Purification Kit was used to extract RNA from 250 µL of cell culture supernatants employing the Maxwell® RSC Instrument (Promega, Madison, WI, USA). The remaining supernatant was conveniently stored at −80 ◦ C for the TCID50

(618) Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cellsCell stem cellJune 3, 2021Breunig M, Merkle J, Wagner M, Melzer MK, Barth TFE, Engleitner T, Krumm J, Wiedenmann S, Cohrs CM, Perkhofer L, et al.added and samples were incubated for 30 min at 65°C, cooled down on ice and further processed on a Maxwell RSC instrument according to manufacturer’s instructions. Eluted DNA was quantified using Qubit dsDNA HS reagents (Thermo

(619) Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19Diagnostic Microbiology and Infectious DiseaseJune 1, 2021Joost Boeckmans, Reinoud Cartuyvels, Petra Hilkens, Liesbeth Bruckers, Koen Magerman, Luc Waumans, Marijke Raymaekerssamples were analyzed with one of the following 3 methods:The first method included RNA extraction on the Maxwell RSC Instrument (Promega) using the RSC Viral TNA kit (Promega) according to the manufacturers’ instructions. An extraction

(620) Ethnic and trans-ethnic genome-wide association studies identify new loci influencing Japanese Alzheimerâ€™s disease riskTranslational PsychiatryJune 1, 2021Daichi Shigemizu, Risa Mitsumori, Shintaro Akiyama, Akinori Miyashita, Takashi Morizono, Sayuri Higaki, Yuya Asanomi, Norikazu Hara, Gen Tamiya, Kengo Kinoshita, Takeshi Ikeuchi, Shumpei Niida, Kouichi Ozakiwas 1.37:1. Genomic DNA was extracted from peripheral blood leukocytes by standard protocols using a Maxwell RSC Instrument (Maxwell RSC Buffy Coat DNA Kit, Promega, USA). All subjects were of Japanese origin and provided informed

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