Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(521) SWAN pathway-network identification of common aneuploidy-based oncogenic driversNucleic Acids ResearchApril 22, 2022Robert R Bowers, Christian M Jones, Edwin A Paz, John K Barrows, Kent E Armeson, David T Long, Joe R Delaneyplate. Whole-exome sequencing and data processing Samples were processed using a Promega Maxwell RSC Instrument (AS4500) and Maxwell RSC Tissue DNA kit (AS1610) to obtain purified DNA. DNA was sent to GENEWIZ for whole-exome

(522) The Clinically Actionable Molecular Profile of Early versus Late-Stage Non-Small Cell Lung Cancer, an Individual Age and Sex Propensity-Matched Pair AnalysisCurrent OncologyApril 11, 2022Anna L. McGuire, Melissa K. McConechy, Barb L. Melosky, John C. English, James J. Choi, Defen Peng, John Yee, Benjamin L. S. Furman, Rosalia Aguirre Hernandez, Pedro Feijao, et al.the manufacturer’s instructions. RNA was extracted using the Promega RNA FFPE kit using the Promega Maxwell RSC instrument. The Canexia Health Find ItTM assay, a FPPE solid tumor DNA-based assay, and the RNA-based Fusions assay

(523) A multi‐tissue view on telomere dynamics and postnatal growthJournal of Experimental Zoology Part A: Ecological and Integrative PhysiologyApril 1, 2022Sarah E. Wolf, Kimberly A. RosvallCriscuolo et al., 2009). We extracted DNA from blood, brain, liver, and adrenals. We used the automated Maxwell® RSC Instrument (Promega, Madison, WI) and Whole Blood DNA Kit (Promega no. AS1520) to extract DNA from ≤25μL whole

(524) Gentamicin at sub-inhibitory concentrations selects for antibiotic resistance in the environmentISME CommunicationsMarch 30, 2022Concepcion Sanchez-Cid, Alexandre Guironnet, Christoph Keuschnig, Laure Wiest, Emmanuelle Vulliet, Timothy M. Vogel1 min, 300 µl of supernatant were added to 300 µl of Lysis Buffer (Promega) and purified using the Maxwell® RSC Instrument and the Maxwell® RSC PureFood GMO and Authentication Kit (Promega). DNA was eluted in 100 µl of Elution Buffer

(525) Effect of differences in light source environment on transcriptome of leaf lettuce (Lactuca sativa L.) to optimize cultivation conditionsPLOS ONEMarch 29, 2022Soichiro Nagano et al.separate tube. Total RNA was extracted using a Maxwell RSC Plant RNA Kit (Promega, Madison, WI, USA) and a Maxwell RSC Instrument (Promega). A tube containing total RNA and PrimeScript RT reagent Kit (Takara Bio) was heated in a block

(526) 15-F2t-Isoprostane Favors an Anti-Inflammatory Phenotype in RAW 264.7 Macrophages during Endotoxin ChallengeAntioxidants (Basel)March 19, 2022Ashley K. Putman, G. Andres Contreras°C pending RNA extraction within 1 mo of collection. Extraction of RNA occurred utilizing a Promega Maxwell RSC Instrument following the manufacturer’s protocol. Quantification and the quality of RNA was assessed with the Nucleid

(527) RNA-Seq Analyses Reveal Roles of the HVCN1 Proton Channel in Cardiac pH HomeostasisFrontiers in Cell and Developmental BiologyMarch 16, 2022Xin Wu, Yawei Li, Mark Maienschein-Cline, Leonid Feferman, Longjun Wu, Liang HongMaxwell® RSC simplyRNA Cells Kit (Promega AS1390) based on manufacturer’s instructions, using Maxwell® RSC Instrument (Promega AS4500), and then RNA was quantified using Qubit 4.0 Fluorometer (Invitrogen) with the Qubit RNA HS Assay Kit

(528) Paper-based immunoassay based on 96-well wax-printed paper plate combined with magnetic beads and colorimetric smartphone-assisted measure for reliable detection of SARS-CoV-2 in salivaBiosensors and BioelectronicsMarch 15, 2022Laura Fabiani, Vincenzo Mazzaracchio, Danila Moscone, Silvia Fillo, Riccardo De Santis, Anella Monte, Donatella Amatore, Florigio Lista, Fabiana Arduiniextracted from 300 μL of swab medium using a Maxwell RSC Viral Total Nucleic Acid Purification Kit on Maxwell RSC Instrument (Promega, USA). Total nucleic acid was eluted in a final volume of 50 μL of nuclease-free water. Then,

(529) The molecular hallmarks of primary and secondary vitreoretinal lymphomaBlood AdvancesMarch 8, 2022Irina Bonzheim et al.remaining vitrectomy material as previously described18 or using the Maxwell RSC DNA FFPE Kit and the Maxwell RSC Instrument (Promega, Madison, WI) according to the manufacturer’s instructions. PCR for the detection of IGH gene

(530) Molecular Characterization of Salmonella Detected along the Broiler Production Chain in Trinidad and TobagoMicroorganismsMarch 6, 2022Anisa Sarah Khan, Rian Ewald Pierneef, Narjol Gonzalez-Escalona, Meghan Maguire, Cong Li, Gregory H. Tyson, Sherry Ayers, Karla Georges, Woubit Abebe, Abiodun Adewale AdesiyunDNA Extraction and Sequencing DNA was extracted using the Maxwell RSC cultured cells DNA kit with a Maxwell RSC instrument (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocols for Gram-negative bacteria

(531) Sequencing of BRCA1/2-alterations using NGS-based technology: annotation as a challengeOncotargetMarch 3, 2022Silvana Ebner, Ria Winkelmann, Saskia Martin, Jens KÃ¶llermann, Peter J. Wild, Melanie Demesfor all three workflows. The purification of DNA from the FFPE tissue samples was performed using the Maxwell® RSC Instrument (Promega Corporation) with the Maxwell® RSC FFPE Plus DNA Kit (Promega Corporation). Therefore, the

(532) CAF-Associated Paracrine Signaling Worsens Outcome and Potentially Contributes to Chemoresistance in Epithelial Ovarian CancerFrontiers in OncologyMarch 3, 2022Michael Wessolly, Elena Mairinger, Sabrina Borchert, Agnes Bankfalvi, Pawel Mach, Kurt Werner Schmid, Rainer Kimmig, Paul Buderath, Fabian Dominik Mairingercommenced. 2.2.2 RNA Isolation RNA was isolated in a semi-automatic workflow with the help of the Maxwell® RSC Instrument (Promega, Wisconsin, USA) using a Maxwell® RSC RNA FFPE kit (AS1440, Promega, Wisconsin, USA). The process

(533) Detection of SARS-CoV-2 genome on inanimate surfaces in COVID-19 intensive care units and emergency care cohortBrazilian Journal of MicrobiologyMarch 1, 2022Thabata Coaglio Lucas, Cristiane Rocha Fagundes Moura, Raquel Aparecida Monteiro, ValÃ©ria da Silva Baracho, Cintia Maria Rodrigues, Kamila Lorene Soares Rocha, Paulo Henrique da Cruz Ferreira, Thyago JosÃ© Silva, Etel Rocha-Vieira, Danilo Bretas de Oliveirato the manufacturer instructions, using the Maxwell® System: Maxwell® RSC simply RNA Cells Kit and Maxwell® RSC Instrument (Promega, Wisconsin, USA). Briefly, 200 μL of the sample was added to 250 μL of Lysis Buffer (Lysis Buffer

(534) Spatial and temporal distribution of SARS-CoV-2 diversity circulating in wastewaterWater ResearchMarch 1, 2022Alba PÃ©rez-CataluÃ±a, Ãlvaro Chiner-Oms, Enric Cuevas-Ferrando, Azahara DÃaz-Reolid, Irene FalcÃ³, Walter Randazzo, InÃ©s GirÃ³n-GuzmÃ¡n, Ana Allende, MarÃa A. Bracho, IÃ±aki Comas, et al.transferred to the loading cartridge and 300 μL of lysis buffer added. The cartridge was then loaded in the Maxwell® RSC Instrument (Promega) using the “Maxwell RSC Viral total Nucleic Acid” running program for the nucleic acid extraction.

(535) Sequencing Depth Has a Stronger Effect than DNA Extraction on Soil Bacterial Richness DiscoveryBiomoleculesFebruary 25, 2022Concepcion Sanchez-Cid, Romie Tignat-Perrier, Laure Franqueville, Laurence DelauriÃ¨re, Trista Schagat, Timothy M. Vogelextraction method described by Griffiths et al. [29], as well as a new semiautomated protocol in which the Maxwell RSC Instrument (Promega, Madison, WI, USA) and a prototype version of the Maxwell RSC Fecal Microbiome DNA Kit (Promega)

(536) Undetected αKlotho in serum is associated with the most aggressive phenotype of breast cancerMolecular and Clinical OncologyFebruary 25, 2022Bruna Cristina Borges, Pedro Augusto Do Amaral, Luiz Ricardo Soldi, Victor Luigi Costa Silva, Fernanda Carvalho De Souza, Felipe Andrés Cordeiro Da Luz, Rogério Agenor De Araújo, Marcelo José Barbosa SilvaRT Total RNA was first extracted using the Total SV RNA Isolation System kit (cat. no. Z3100) in a Maxwell® RSC Instrument (Promega Corporation), and complementary DNA (cDNA) was obtained from total RNA using the GoScript™

(537) Proteasomal Processing Immune Escape Mechanisms in Platinum-Treated Advanced Bladder CancerGenes (Basel)February 25, 2022Michael Wessolly, Fabian D. Mairinger, Thomas Herold, Boris Hadaschik, Tibor Szarvas, Henning ReisAX4920 custom, Promega Maxwell, Fitchburg, MA, USA) on a Maxwell RSC device (Maxwell RSC Instrument AS4500, Promega Maxwell) according to the manufacturer’s manual (R29X) with minor adaptions. Proteinase K end

(538) Cyclosporine A Inhibits Viral Infection and Release as Well as Cytokine Production in Lung Cells by Three SARS-CoV-2 VariantsMicrobiology spectrumFebruary 23, 2022Fenizia C, Galbiati S, Vanetti C, Vago R, Clerici M, Tacchetti C, Daniele T.Maxwell RSC Viral Total Nucleic Acid purification kit was used to extract RNA from 250 μL of cell culture supernatants employing the Maxwell RSC Instrument (Promega, Madison, WI, USA). The remaining supernatant was conveniently stored at −80°C for the TCID50

(539) FYCO1 Frameshift Deletion in Wirehaired Pointing Griffon Dogs with Juvenile CataractGenes (Basel)February 11, 2022Gabriela Rudd Garces, Matthias Christen, Robert Loechel, Vidhya Jagannathan, Tosso Leebisolation. Genomic DNA was isolated from 500 µL of EDTA blood with the Maxwell RSC Whole-Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.3. Whole-Genome Sequencing of an Affected Dog An Illumina TruSeq

(540) Quantitative and Functional Assessment of the Influence of Routinely Used Cryopreservation Media on Mononuclear Leukocytes for Medical ResearchInternational Journal of Molecular SciencesFebruary 7, 2022Patrick Haider, Timothy Hoberstorfer, Manuel Salzmann, Michael B. Fischer, Walter S. Speidl, Johann Wojta, Philipp J. HohensinnerGene Expression Analysis RNA from MNC lysates was prepared using Maxwell RSC simplyRNA Tissue kits on a Maxwell RSC Instrument (both Promega, Madison, WI, USA). To create cDNA from the extracted RNA, 10 μL RNA was added to 10 μL

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