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Automated DNA/RNA Purification Sample Preparation Workstations
Maxwell RSC Instrument
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Maxwell RSC Instrument
Maxwell RSC Instrument
Maxwell RSC Instrument from Promega
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Citations:
(849)
Figures:
(1)
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Maxwell RSC Instrument
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Description
The Maxwell RSC Instrument is a platform for automated extraction of nucleic acid from a range of sample types. Extraction methods use sample lysis and binding to paramagnetic particles followed by washing steps and elution.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC Instrument
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS4500
Quantity
EA
Processing Time
30 to 60 min (depending on sample type and method)
Capacity
Up to 16
Dimensions
(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV Lamp
Avg. lifetime approximately 6,000 hrs
Molecular Weight
~85kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (849)
(481)
Characterisation of SARS-CoV-2 genomic variation in response to molnupiravir treatment in the AGILE Phase IIa clinical trial
Nature Communications
November 26, 2022
I’ah Donovan-Banfield, Rebekah Penrice-Randal, Hannah Goldswain, Aleksandra M. Rzeszutek, Jack Pilgrim, Katie Bullock, Geoffrey Saunders, Josh Northey, Xiaofeng Dong, Yan Ryan,
et al.
from the nasopharyngeal swabs by the GCP Laboratory Facility at the University of Liverpool using a
Maxwell® RSC instrument
, an automated nucleic acid extraction instrument (
Promega
, USA). Aliquots of surplus RNA were provided
(482)
Induced pluripotent stem cells and cerebral organoids from the critically endangered Sumatran rhinoceros
iScience
November 18, 2022
Zywitza V, Frahm S, Krüger N, Weise A, Göritz F, Hermes R, Holtze S, Colleoni S, Galli C, Drukker M,
et al
.
genomic DNA (gDNA) automatedly extracted using the
Maxwell RSC Blood DNA Kit
(
Promega
, #
AS1400
) and a
Maxwell RSC instrument
according to the manufacturer’s instructions (quick protocol from point 2 on). gDNA was eluted in 85
(483)
Genomic characterization of intracranial teratomas using whole genome sequencing
Frontiers in Oncology
November 15, 2022
Chao Zhang, Xiaoyu Zhou, Xiang Huang, Xinghua Ding, Yang Wang, Rong Zhang
the
Maxwell RSC Blood DNA Kit
(cat#
AS1400
,
Promega
, Madison, WI, USA) on a Maxwell RSC system (cat#
AS4500
,
Promega
). For FFPE sections, DNA was extracted using the
MagMAX FFPE DNA/RNA Ultra Kit
(cat#
A31881
,
(484)
Enhanced exon skipping and prolonged dystrophin restoration achieved by TfR1-targeted delivery of antisense oligonucleotide using FORCE conjugation in mdx mice
Nucleic acids research
November 11, 2022
Desjardins CA, Yao M, Hall J, O'Donnell E, Venkatesan R, Spring S, Wen A, Hsia N, Shen P, Russo R,
et al
.
(Cat. No.
A-21208
) antibodies were purchased from
ThermoFisher Scientific
/Invitrogen, Carlsbad, CA. The
Maxwell RSC instrument
(Cat. No.
AS8500
) and Maxwell RSC simplyTissue extraction kit (Cat. No.
AS1340
) were obtained from
Promega
,
(485)
Long-term SARS-CoV-2 surveillance in the wastewater of Stockholm: What lessons can be learned from the Swedish perspective?
Sci Total Environ
November 8, 2022
Mariel Perez-Zabaleta, Amena Archer, Kasra Khatami, Mohammed Hakim Jafferali, Prachi Nandy, Merve Atasoy, Madeleine Birgersson, Cecilia Williams, Zeynep Cetecioglu
concentrated from raw wastewater samples using Maxwell RSC Enviro TNA Promega Kit, and RNA was extracted using
Maxwell RSC Instrument
(
Promega
Biotech AB, Sweden) following the manufacturer's instructions. Briefly, a volume of 40 mL of
(486)
SARS-CoV-2 vertical transmission during the first trimester of pregnancy in asymptomatic women
International Journal of Infectious Diseases
November 1, 2022
Claudio Fenizia, Claudia Vanetti, Francesca Rana, Gioia Cappelletti, Irene Cetin, Mara Biasin, Valeria Savasi
obtained. Plasma samples were collected from the blood of all enrolled subjects. RNA was extracted by the
Maxwell® RSC Instrument
with the
Maxwell® RSC Viral Total Nucleic Acid Purification Kit
(
Promega
, Fitchburg, WI, USA). As a
(487)
Recombinant thrombomodulin may protect cardiac capillary endothelial glycocalyx through promoting Glypican-1 expression under experimental endotoxemia
Heliyon
November 1, 2022
Kakino Y, Doi T, Okada H, Suzuki K, Takada C, Tomita H, Asano H, Kano S, Wakayama Y, Okuda T,
et al
.
and rhTM-treated
mice
30 h after LPS administration (n = 3 each). The total RNA was extracted using a
Maxwell RSC instrument
with a simple RNA tissue kit (
Promega
, Fitchburg, WI, USA).
Gene expression analysis
of the RNA samples
(488)
An external quality assessment for the molecular testing of the SARS-CoV-2 virus genome in Zhejiang Province, China
Diagnostic Microbiology and Infectious Disease
November 1, 2022
Junhang Pan, Hao Yan, Zhen Li, Xiuyu Lou, Haiyan Mao, Wen Shi, Wenwu Yao, Yanjun Zhang
(Bioperfectus)D: TGuide S32 (TIANGEN)E: MagMax Express (Thermo)F: Maxwell 16/Maxwell RSC 48/Maxwell RSC
AS4500
(
Promega
)G: MagNA Pure 96/MagNA Pure LC 2.0 (Roche)H: GenAct NE-48/GenAct NL-48 (GeneoDx)I: NX-48 (Genolution)J:
(489)
The oncogenic fusion protein DNAJB1-PRKACA can be specifically targeted by peptide-based immunotherapy in fibrolamellar hepatocellular carcinoma
Nature communications
October 27, 2022
Bauer J, Köhler N, Maringer Y, Bucher P, Bilich T, Zwick M, Dicks S, Nelde A, Dubbelaar M, Scheid J,
et al
.
was extracted from macrodissected 5 µm paraffin sections using the
Maxwell® RSC RNA FFPE Kit
and the
Maxwell® RSC Instrument
(
Promega
, Madison, WI, USA) according to the manufacturer’s instructions. Reverse transcription of RNA
(490)
Integrative genomic analysis of drug resistance in MET exon 14 skipping lung cancer using patient-derived xenograft models
Frontiers in Oncology
October 21, 2022
Yunhua Xu, Linping Gu, Yingqi Li, Ruiying Zhao, Hong Jian, Wenhui Xie, Liu Liu, Huiwen Wu, Fang Ren, Yuchen Han,
et al.
the
Maxwell RSC Blood DNA Kit
(cat#
AS1400
,
Promega
, Madison, WI, USA) on a Maxwell RSC system (cat#
AS4500
,
Promega
). DNA of FFPE tissue was extracted using the
MagMAX FFPE DNA/RNA Ultra Kit
(cat#
A31881
,
ThermoFisher
,
(491)
Cuprizone feeding induces swollen astrocyte endfeet
Pflügers Archiv - European Journal of Physiology
October 15, 2022
Petra Fallier-Becker, Irina Bonzheim, Friederike Pfeiffer
cryosections
that have been embedded in Tissue Tek (experiment 2) using the
Maxwell RSC RNA FFPE Kit
and the
Maxwell RSC Instrument
(
Promega
, Madison, WI, USA) according to the manufacturer’s instructions. For FFPE material, sections
(492)
Community-Based SARS-CoV-2 Testing Using Saliva or Nasopharyngeal Swabs to Compare the Performance of Weekly COVID-19 Screening to Wastewater SARS-CoV-2 Signals
ACS ES&T Water
October 14, 2022
Zhengchun Lu, Amanda E. Brunton, Maedeh Mohebnasab, Anthony Deloney, Kenneth J. Williamson, Blythe A. Layton, Scott Mansell, Alice Brawley-Chesworth, Peter Abrams, Kimberly A. Wilcox,
et al.
previously described.23 Briefly, RNA was extracted using one of the three different automated machines:
Maxwell RSC Instrument
(
Promega
, Madison, MI), MagNA Pure 96 System (Roche Diagnostics, Pleasanton, CA), and KingFisher Flex
(493)
ACADM Frameshift Variant in Cavalier King Charles Spaniels with Medium-Chain Acyl-CoA Dehydrogenase Deficiency
Genes (Basel)
October 13, 2022
Matthias Christen, Jos Bongers, Déborah Mathis, Vidhya Jagannathan, Rodrigo Gutierrez Quintana, Tosso Leeb
DNA Extraction Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a
Maxwell RSC instrument
(
Promega
, Dübendorf, Switzerland). In addition to the affected
dog
, 162 blood samples from CKCS, which
(494)
Genetic variants for prediction of gestational diabetes mellitus and modulation of susceptibility by a nutritional intervention based on a Mediterranean diet
Front Endocrinol (Lausanne)
October 13, 2022
Ana Ramos-Levi, Ana Barabash, Johanna Valerio, Nuria GarcÃa de la Torre, Leire Mendizabal, Mirella Zulueta, Maria Paz de Miguel, Angel Diaz, Alejandra Duran, Cristina Familiar,
et al.
analysis Genomic DNA was extracted from EDTA-stabilized blood samples taken during the
OGTT
using the
Maxwell RSC instrument
(
Promega
, Dubendorf, Switzerland).
Genotyping
was performed by IPLEX MassARRAY
PCR
using the Agena platform
(495)
Interleukinâ€4 receptor alpha signaling regulates monocyte homeostasis
The FASEB Journal
October 1, 2022
Patrick Haider, Julia B. Kralâ€Pointner, Manuel Salzmann, Florian Moik, Sonja Bleichert, Waltraud C. Schrottmaier, Christoph Kaun, Mira Brekalo, Michael B. Fischer, Walter S. Speidl,
et al.
Quantitative PCR
RNA from
cell culture
lysates was prepared using Maxwell RSC simplyRNA Tissue kits on a
Maxwell RSC Instrument
(both
Promega
, Madison, WI, USA). cDNA was created using a
1:1
Oligo(dT)/Random Primer Reverse Transcriptase
(496)
Physiological intron retaining transcripts in the cytoplasm abound during human motor neurogenesis
Genome Research
October 1, 2022
Marija Petrić Howe, Hamish Crerar, Jacob Neeves, Jasmine Harley, Giulia E. Tyzack, Pierre Klein, Andres Ramos, Rickie Patani, Raphaëlle Luisier
clustering
. RNA extraction RNA was extracted using the Maxwell RSC simplyCells RNA kit (Promega) and the
Maxwell RSC instrument
(
Promega
), according to the manufacturer's instructions. Contaminating genomic DNA was removed by DNase
(497)
Pet Reptiles in Poland as a Potential Source of Transmission of Salmonella
Pathogens
September 29, 2022
Marta Dec, Magdalena ZajÄ…c, Andrzej Puchalski, Klaudiusz Szczepaniak, Renata Urban-Chmiel
Cultured Cells
(
AS1620
Promega
, Madison, WI, USA) according to the manufacturer’s instructions with the
Maxwell® RSC Instrument
(
Promega
, Madison, WI, USA). For yield and purity checks, all samples were measured with NanoDropTM
(498)
Development, Evaluation, and Implementation of a House-Made Targeted Next-Generation Sequencing Spoligotyping in a French Laboratory
International Journal of Molecular Sciences
September 25, 2022
Charlotte Genestet, Yannick Baffert, Maxime Vallée, Albin Bernard, Yvonne Benito, Gérard Lina, Elisabeth Hodille, Oana Dumitrescu, Lyon TB Study Group
Silico
WGS
-Based Spoligotyping For MTBC
WGS
, genomic DNA was purified from cleared lysates using the
Maxwell RSC Instrument
(
Promega
, Madison, WI, USA) automated DNA extraction system and the
Maxwell RSC Blood DNA Kit
(
Promega
).
(499)
High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistance
Nature communications
September 22, 2022
Cortés-López M, Schulz L, Enculescu M, Paret C, Spiekermann B, Quesnel-Vallières M, Torres-Diz M, Unic S, Busch A, Orekhova A,
et al
.
were acquired with GBox instrument (Syngene).
Quantitative real-time PCR
RNA was extracted using the
Maxwell RSC Instrument
(
Promega
) and the
Maxwell® RSC simplyRNA Cells Kit
(
AS1390
,
Promega
). 0.5 µg or RNA were reverse-transcribed
(500)
Polymorphism of Drug Transporters, Rather Than Metabolizing Enzymes, Conditions the Pharmacokinetics of Rasagiline
Pharmaceutics
September 21, 2022
Pablo Zubiaur, Miriam Matas, Samuel MartÃn-VÃlchez, Paula Soria-Chacartegui, Gonzalo Villapalos-GarcÃa, Laura Figueiredo-Tor, SofÃa Calleja, Marcos Navares-Gómez, Alejandro de Miguel, Jesús Novalbos,
et al.
reconstituted with 0.9% sodium chloride solution and 500 µL were used for DNA extraction with an automated
Maxwell® RSC instrument
(
Promega
, Waltham, MA, USA). A
Qubit® 3.0 fluorometer
(
ThermoFisher
, Waltham, MA, USA) was used to
measure
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Maxwell RSC Instrument from Promega
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