Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(481) Physical separation of haplotypes in dikaryons allows benchmarking of phasing accuracy in Nanopore and HiFi assemblies with Hi-C dataGenome BiologyDecember 1, 2022Hongyu Duan, Ashley W. Jones, Tim Hewitt, Amy Mackenzie, Yiheng Hu, Anna Sharp, David Lewis, Rohit Mago, Narayana M. Upadhyaya, John P. Rathjen, et al.and 9 days post inoculation (dpi) was extracted using the Promega Maxwell® RSC Plant RNA Kit with a Maxwell® RSC instrument (Promega.com.au). The spores were induced for germination by placing on the surface of milli-Q water,

(482) Molecular cytogenetic characterization of partial trisomy of the long arm of chromosome 11 in a patient with multiple congenital anomaliesMolecular CytogeneticsDecember 1, 2022Austin Walker, Xianfu Wang, Young Mi Kim, Xianglan Lu, Ashley Taylor, Danielle Demarzo, Shibo Li, Hui Pang(ISCN) 2016. Chromosomal microarray analysis DNA samples were extracted from peripheral blood using Maxwell RSC Instrument and Maxwell RSC Blood DNA Kit (Promega, 2800 Woods Hollow Road, Madison, WI 53711 USA) following manufacturer’s

(483) Detecting structural variations with precise breakpoints using low-depth WGS data from a single oxford nanopore MinION flowcellScientific ReportsDecember 1, 2022Henry C. M. Leung, Huijing Yu, Yifan Zhang, Wing Sze Leung, Ivan F. M. Lo, Ho Ming Luk, Wai-Chun Law, Ka Kui Ma, Chak Lim Wong, Yat Sing Wong, et al.the real patient data, DNA was extracted from EDTA blood using Maxwell RSC Whole Blood DNA Kit with Maxwell RSC Instrument (Promega, Spain) and stored at − 80 °C upon sequencing. All DNA samples were prepared using an in-house

(484) Toxoplasma gondii and Rickettsia spp. in ticks collected from migratory birds in the Republic of KoreaScientific ReportsDecember 1, 2022A.-Tai Truong, Mi-Sun Yoo, Subin Min, Ji-Yeon Lim, Hyun-Ji Seo, Heung-Chul Kim, Sung-Tae Chong, Terry A. Klein, Chang-uk Park, Sook-Young Cho, et al.extraction using Maxwell® RSC Viral Total Nucleic Acid Purification Kits (Promega, USA) and an automated Maxwell RSC Instrument (Promega). The procedure of isolation was done according to the manufacturer’s instructions. Extracted

(485) Utility of ultra-rapid real-time PCR for detection and prevalence of Rickettsia spp. in ticksBMC Veterinary ResearchDecember 1, 2022A-Tai Truong, Bo-Ram Yun, Mi-Sun Yoo, Jiyeon Lim, Subin Min, Soon-Seek Yoon, Young-Min Yun, Jong-Taek Kim, Yun Sang Choacid was extracted using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit on the automated Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Finally, 50 μl of total nucleic

(486) Rapid detection of single nucleotide polymorphisms using the MinION nanopore sequencer: a feasibility study for perioperative precision medicineJA Clinical ReportsDecember 1, 2022Yoshiteru Tabata, Yoshiyuki Matsuo, Yosuke Fujii, Atsufumi Ohta, Kiichi Hirota(AS1400, Promega, Madison, WI, USA) on the Maxwell RSC automated nucleic acid purification platform (AS4500, Promega). Briefly, the sample (0.3 mL) was mixed with lysis buffer (0.3 mL) and proteinase K solution

(487) Utility of plasma circulating tumor DNA and tumor DNA profiles in head and neck squamous cell carcinomaScientific ReportsDecember 1, 2022Nobuyuki Chikuie, Yuji Urabe, Tsutomu Ueda, Takao Hamamoto, Takayuki Taruya, Takashi Kono, Kohei Yumii, Sachio TakenoMaxwell RSC Blood DNA Kit (Promega)38. Nucleic acids were automatically extracted from both samples using the Maxwell RSC Instrument. DNA concentrations were measured using a Qubit dsDNAHS Assay Kit (Thermo Fisher Scientific, Waltham,

(488) Limited movement of an avian hybrid zone in relation to regional variation in magnitude of climate changeMolecular EcologyDecember 1, 2022Alana Alexander, Mark B. Robbins, Jesse Holmes, Robert G. Moyle, A. Townsend Petersonextraction DNA was extracted from ~15 mg of tissue using a Blood DNA kit and manufacturer protocols on a Maxwell RSC instrument (Promega), with the following modifications: before loading into the cartridge, samples were lysed for

(489) ECNano: A cost-effective workflow for target enrichment sequencing and accurate variant calling on 4800 clinically significant genes using a single MinION flowcellBMC Medical GenomicsDecember 1, 2022Amy Wing-Sze Leung, Henry Chi-Ming Leung, Chak-Lim Wong, Zhen-Xian Zheng, Wui-Wang Lui, Ho-Ming Luk, Ivan Fai-Man Lo, Ruibang Luo, Tak-Wah Lamwere extracted from EDTA blood samples. The automated DNA extraction was performed using the Promega Maxwell® RSC Instrument (Promega, Madison, WA, USA) with the Promega Maxwell® RSC Blood DNA Kit. The pure standard DNA sample

(490) Mitochondrial fission factor (MFF) frameshift variant in Bullmastiffs with mitochondrial fission encephalopathyAnimal GeneticsDecember 1, 2022Matthias Christen, Rodrigo Gutierrez‐Quintana, Helene Vandenberghe, Adriana Kaczmarska, Jacques Penderis, Roberto José‐López, Angie Rupp, Ian R. Griffiths, Vidhya Jagannathan, Tosso Leebcases. DNA extraction Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega). The same instrument was used for DNA isolation from FFPE tissue samples with the Maxwell RSC DNA FFPE kit

(491) Droplet digital PCR-based analyses for robust, rapid, and sensitive molecular diagnostics of gliomasActa Neuropathologica CommunicationsDecember 1, 2022Marietta Wolter, Jörg Felsberg, Bastian Malzkorn, Kerstin Kaulich, Guido ReifenbergerGermany), the GeneRead DNA FFPE Kit (Qiagen), or the Maxwell® RSC FFPE Plus DNA Kit together with the Maxwell® RSC instrument (Promega, Mannheim, Germany). DNA extraction from frozen tissue samples was performed with the PureLink™ Genomic DNA Mini Kit

(492) The basis and design for time‐restricted eating compared with daily calorie restriction for weight loss and colorectal cancer risk reduction trial ( TRE‐CRC trial)Obesity (Silver Spring)December 1, 2022Kelsey Gabel, Marian L. Fitzgibbon, Cemal Yazici, Peter Gann, Maria Sverdlov, Grace Guzman, Zhengjia Chen, Andrew McLeod, Alyshia Hamm, Krista A. Varady, et al.gene expression Total RNA will be extracted from tissue using the using Maxwell RSC simplyRNA Tissue Kit with the Maxwell RSC Instrument (Promega). Prior to gene expression analysis, RNA quality and quantity will be determined using TapeStation4200

(493) DNA methylation-based classification of sinonasal tumorsNature CommunicationsNovember 28, 2022Philipp Jurmeister, Stefanie Glöß, Renée Roller, Maximilian Leitheiser, Simone Schmid, Liliana H. Mochmann, Emma Payá Capilla, Rebecca Fritz, Carsten Dittmayer, Corinna Friedrich, et al.light microscopy of hematoxylin and eosin-stained sections. Semi-automated DNA extraction was performed on the Maxwell RSC Instrument using the Maxwell RSC FFPE Plus DNA Purification Kit (Custom, AX4920; Promega). Extracted total DNA

(494) Characterisation of SARS-CoV-2 genomic variation in response to molnupiravir treatment in the AGILE Phase IIa clinical trialNature CommunicationsNovember 26, 2022I’ah Donovan-Banfield, Rebekah Penrice-Randal, Hannah Goldswain, Aleksandra M. Rzeszutek, Jack Pilgrim, Katie Bullock, Geoffrey Saunders, Josh Northey, Xiaofeng Dong, Yan Ryan, et al.from the nasopharyngeal swabs by the GCP Laboratory Facility at the University of Liverpool using a Maxwell® RSC instrument, an automated nucleic acid extraction instrument (Promega, USA). Aliquots of surplus RNA were provided

(495) Induced pluripotent stem cells and cerebral organoids from the critically endangered Sumatran rhinocerosiScienceNovember 18, 2022Zywitza V, Frahm S, Krüger N, Weise A, Göritz F, Hermes R, Holtze S, Colleoni S, Galli C, Drukker M, et al.genomic DNA (gDNA) automatedly extracted using the Maxwell RSC Blood DNA Kit (Promega, #AS1400) and a Maxwell RSC instrument according to the manufacturer’s instructions (quick protocol from point 2 on). gDNA was eluted in 85

(496) Genomic characterization of intracranial teratomas using whole genome sequencingFrontiers in OncologyNovember 15, 2022Chao Zhang, Xiaoyu Zhou, Xiang Huang, Xinghua Ding, Yang Wang, Rong Zhangthe Maxwell RSC Blood DNA Kit (cat# AS1400, Promega, Madison, WI, USA) on a Maxwell RSC system (cat# AS4500, Promega). For FFPE sections, DNA was extracted using the MagMAX FFPE DNA/RNA Ultra Kit (cat# A31881,

(497) Enhanced exon skipping and prolonged dystrophin restoration achieved by TfR1-targeted delivery of antisense oligonucleotide using FORCE conjugation in mdx miceNucleic acids researchNovember 11, 2022Desjardins CA, Yao M, Hall J, O'Donnell E, Venkatesan R, Spring S, Wen A, Hsia N, Shen P, Russo R, et al.(Cat. No. A-21208) antibodies were purchased from ThermoFisher Scientific/Invitrogen, Carlsbad, CA. The Maxwell RSC instrument (Cat. No. AS8500) and Maxwell RSC simplyTissue extraction kit (Cat. No. AS1340) were obtained from Promega,

(498) Long-term SARS-CoV-2 surveillance in the wastewater of Stockholm: What lessons can be learned from the Swedish perspective?Sci Total EnvironNovember 8, 2022Mariel Perez-Zabaleta, Amena Archer, Kasra Khatami, Mohammed Hakim Jafferali, Prachi Nandy, Merve Atasoy, Madeleine Birgersson, Cecilia Williams, Zeynep Ceteciogluconcentrated from raw wastewater samples using Maxwell RSC Enviro TNA Promega Kit, and RNA was extracted using Maxwell RSC Instrument (Promega Biotech AB, Sweden) following the manufacturer's instructions. Briefly, a volume of 40 mL of

(499) SARS-CoV-2 vertical transmission during the first trimester of pregnancy in asymptomatic womenInternational Journal of Infectious DiseasesNovember 1, 2022Claudio Fenizia, Claudia Vanetti, Francesca Rana, Gioia Cappelletti, Irene Cetin, Mara Biasin, Valeria Savasiobtained. Plasma samples were collected from the blood of all enrolled subjects. RNA was extracted by the Maxwell® RSC Instrument with the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Fitchburg, WI, USA). As a

(500) An external quality assessment for the molecular testing of the SARS-CoV-2 virus genome in Zhejiang Province, ChinaDiagnostic Microbiology and Infectious DiseaseNovember 1, 2022Junhang Pan, Hao Yan, Zhen Li, Xiuyu Lou, Haiyan Mao, Wen Shi, Wenwu Yao, Yanjun Zhang(Bioperfectus)D: TGuide S32 (TIANGEN)E: MagMax Express (Thermo)F: Maxwell 16/Maxwell RSC 48/Maxwell RSC AS4500 (Promega)G: MagNA Pure 96/MagNA Pure LC 2.0 (Roche)H: GenAct NE-48/GenAct NL-48 (GeneoDx)I: NX-48 (Genolution)J:

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