Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(581) MIA3 Splice Defect in Cane Corso Dogs with Dental-Skeletal-Retinal Anomaly (DSRA)Genes (Basel)September 25, 2021Matthias Christen, HenriÃ«tte Booij-Vrieling, Jelena Oksa-Minalto, Cynthia de Vries, Alexandra Kehl, Vidhya Jagannathan, Tosso LeebGenotyping Genomic DNA was isolated from EDTA blood samples with the Maxwell RSC Whole Blood DNA Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). DNA from 12 affected and 8 unaffected animals was genotyped on illumina_HD

(582) PRKG2 Splice Site Variant in Dogo Argentino Dogs with Disproportionate DwarfismGenes (Basel)September 24, 2021Gabriela Rudd Garces, Maria Elena Turba, Myriam Muracchini, Alessia Diana, Vidhya Jagannathan, Fabio Gentilini, Tosso LeebDNA Isolation Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.3. Linkage and Homozygosity Mapping The Dogo Argentino family,

(583) MYO5A Frameshift Variant in a Miniature Dachshund with Coat Color Dilution and Neurological Defects Resembling Human Griscelli Syndrome Type 1Genes (Basel)September 23, 2021Matthias Christen, Madeleine de le Roi, Vidhya Jagannathan, Kathrin Becker, Tosso LeebExtraction Genomic DNA was isolated from the EDTA blood with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.5. Whole-Genome Sequencing An Illumina TruSeq PCR-free DNA library

(584) Analysis of Telomere Maintenance Related Genes Reveals NOP10 as a New Metastatic-Risk Marker in Pheochromocytoma/ParagangliomaCancers (Basel)September 23, 2021María Monteagudo et al.samples using the Maxwell® RSC DNA Formalin-fixed paraffin embedded Kit (Promega, Madison, WI, USA) and a Maxwell® RSC Instrument (Promega). DNA from frozen tissue was extracted with DNeasy® Blood and Tissue Kit (Qiagen, Hilden, Germany),

(585) Prolyl isomerase Pin1 plays an essential role in SARS-CoV-2 proliferation, indicating its possibility as a novel therapeutic targetScientific reportsSeptember 17, 2021Yamamotoya T, Nakatsu Y, Kanna M, Hasei S, Ohata Y, Encinas J, Ito H, Okabe T, Asano T, Sakaguchi T.Sendai, Japan) as a standard. Cellular RNA in the infected VeroE6/TMPRSS2 cells was prepared using the Maxwell RSC instrument (Promega Corp., Madison, WI) according to the manufacturer’s protocol. RT-qPCR for specific amplification

(586) PD-L1 Dependent Immunogenic Landscape in Hot Lung Adenocarcinomas Identified by Transcriptome AnalysisCancers (Basel)September 11, 2021Jutta Kirfel et al.mRNA isolation and quantification were performed using the Maxwell RSC RNA FFPE Kit together with the Maxwell RSC instrument (Promega, Fitchburg, WI, USA) and the Qubit fluorimeter (ThermoFisher, Waltham, MA, USA), respectively.

(587) The splicing regulator SLU7 is required to preserve DNMT1 protein stability and DNA methylationNucleic acids researchSeptember 7, 2021Recalde M, Gárate-Rascón M, Elizalde M, Azkona M, Latasa MU, Bárcena-Varela M, Sangro B, Fernández-Barrena MG, Ávila MA, Arechederra M, et al.(University of Utah, USA). Total DNA isolation Total DNA from cell lines was extracted using the automated Maxwell RSC Instrument with cultured cells DNA kit (Promega, Madison, WI, USA). DNA purity and concentration were measured

(588) Serum Vitamin D Is Associated with Antioxidant Potential in Peri-Parturient CowsAntioxidants (Basel)September 6, 2021Jaimie M. Strickland, Lauren Wisnieski, Vengai Mavangira, Lorraine M. Sordilloperformed as previously described by our group [41]. The RNA from BAECs was extracted using the (Promega Maxwell RSC instrument, Madison, WI, USA) following the manufacturer’s protocol. Quantitative real-time PCR (qRT-PCR) was performed

(589) Saliva Testing Is Accurate for Early-Stage and Presymptomatic COVID-19Microbiology SpectrumSeptember 3, 2021Abigail J. Johnson et al.or the Promega Maxwell RSC viral total nucleic acid kit (number AS1330; Promega, Madison, WI) on the Maxwell RSC instrument per the manufacturer’s instructions; bridging studies showed equivalent limits of detection between

(590) Screening for extremely rare pathogenic variants of monogenic diabetes using targeted panel sequencingEndocrineSeptember 1, 2021Tomasz PÅ‚oszaj, Karolina Antosik, Paulina Jakiel, Agnieszka ZmysÅ‚owska, Maciej Borowiecreaction Subjects DNA isolation was performed from peripheral blood with a semi-automatic device from the Promega Maxwell® RSC Instrument. The library was prepared in accordance with the Agilent SureSelectQXT Target Enrichment protocol using

(591) Association of Initial Viral Load in Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Patients with Outcome and SymptomsThe American Journal of PathologySeptember 1, 2021Kimon V. Argyropoulos, Antonio Serrano, Jiyuan Hu, Margaret Black, Xiaojun Feng, Guomiao Shen, Melissa Call, Min J. Kim, Andrew Lytle, Brendan Belovarac, et al.included, serving as the internal control. Viral RNA was extracted within using the Promega (Madison, WI) Maxwell RSC Instrument and Swab kit, according to the manufacturer's instructions. All quantitative RT-PCR assays were performed

(592) Prediction of False-Positive Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Molecular Results in a High-Throughput Open-Platform SystemThe Journal of Molecular DiagnosticsSeptember 1, 2021Ryan J. Martinez, Nathan Pankratz, Matthew Schomaker, Jerry Daniel, Kenneth Beckman, Amy B. Karger, Bharat Thyagarajan, Patricia Ferreri, Sophia L. Yohe, Andrew C. Nelson16-sample throughput Promega Maxwell RSC Viral Total Nucleic Acid kit (Promega, Madison, WI) on the Maxwell RSC instrument; iii) the automated 96-sample throughput MagMAX Viral and Pathogen Nucleic Acid Isolation kit on the

(593) Monitoring levels of circulating cell‐free DNA in patients with metastatic colorectal cancer as a potential biomarker of responses to regorafenib treatmentMolecular OncologySeptember 1, 2021Brice Pastor, Thierry André, Julie Henriques, Isabelle Trouilloud, Christophe Tournigand, Marine Jary, Thibault Mazard, Christophe Louvet, Simon Azan, Audrey Bauer, et al.at −20 °C until use if not used immediately. Circulating cfDNA was also extracted from 1 mL plasma (Maxwell® RSC Instrument) using the cfDNA Plasma Kit (Promega Corporation, Madison, WI, USA) in an elution volume of 130 µL.

(594) A Missense Variant in SLC39A4 in a Litter of Turkish Van Cats with Acrodermatitis EnteropathicaGenes (Basel)August 25, 2021Sarah Kiener, Robert Cikota, Monika Welle, Vidhya Jagannathan, Susanne Ã…hman, Tosso Leebbreeds. Genomic DNA was isolated from ETDA blood samples with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.2. Histopathological Examinations Skin biopsies (6 mm punch and

(595) Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of KoreaPathogensAugust 24, 2021Hyun-Ji Seo, A-Tai Truong, Keun-Ho Kim, Ji-Yeon Lim, Subin Min, Heung-Chul Kim, Mi-Sun Yoo, Soon-Seek Yoon, Terry A. Klein, Yun Sang Chotube. After incubating at 56 ℃ for 10 min purification of nucleic acids was done by using an automated Maxwell RSC Instrument (Promega, Madison, WI, USA). Isolated material was stored at −80 °C until further molecular analysis. 4.3.

(596) Variants Affecting the C-Terminal Tail of UNC93B1 Are Not a Common Risk Factor for Systemic Lupus ErythematosusGenes (Basel)August 19, 2021Sarah Kiener, Camillo Ribi, Irene Keller, Carlo Chizzolini, Marten Trendelenburg, Uyen Huynh-Do, Johannes von Kempis, Tosso on behalf of Swiss SLE Cohort Study (SSCS), Tosso on behalf of Swiss SLE Cohort Study (SSCS)[27,28,29]. Genomic DNA was isolated from ETDA blood samples with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.2. UNC93B1 Targeted Sanger Sequencing A 1000 bp PCR amplicon

(597) Co-occurring gain-of-function mutations in HER2 and HER3 modulate HER2/HER3 activation, oncogenesis, and HER2 inhibitor sensitivityCancer CellAugust 9, 2021Ariella B. Hanker et al.and ERBB3 RNA was isolated from CW2 cells using the Maxwell RSC simplyRNA Cells Kit (Promega) on the Maxwell RSC Instrument (Promega). RNA was isolated from SA493 organoids using the Qiagen RNeasy Micro Kit. Reverse transcription

(598) Whole genome sequencing of low input circulating cell‐free DNA obtained from normal human subjectsPhysiological ReportsAugust 4, 2021Julie F. Foley et al.(Maxwell® RSC ccfDNA Plasma Kit, Promega) and eluted with nuclease‐free water (50 μl volume) on a Promega Maxwell® RSC instrument. Sample yield was determined by a fluorometric method (Quantus™ Fluorometer, Promega). Fragmentation

(599) Deletion of the SELENOP gene leads to CNS atrophy with cerebellar ataxia in dogsPLOS GeneticsAugust 2, 2021Matthias Christen, Sandra HÃ¶gler, Miriam Kleiter, Michael Leschnik, Corinna Weber, Denise Thaller, Vidhya Jagannathan, Tosso Leebmethods using the Maxwell RSC Whole Blood DNA and the Maxwell RSC Blood DNA Kits in combination with the Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Linkage analysis and homozygosity mapping Genotype data for the

(600) SARS-CoV-2 genomic surveillance in Costa Rica: Evidence of a divergent population and an increased detection of a spike T1117I mutationInfection, Genetics and EvolutionAugust 1, 2021Jose Arturo Molina-Mora, Estela Cordero-Laurent, Adriana GodÃnez, Melany CalderÃ³n-Osorno, Hebleen Brenes, Claudio Soto-Garita, Cristian PÃ©rez-Corrales, COINGESA-CR Consorcio Interinstitucional de Estudios GenÃ³micos del SARS-CoV-2 Costa Rica, Jan Felix Drexler, Andres Moreira-Soto, et al.in a QIACube extractor or with Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega) in a Maxwell® RSC instrument following the manufacturer's protocol. cDNA for library preparation was then synthesized and purified

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