Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(581) Undetected αKlotho in serum is associated with the most aggressive phenotype of breast cancerMolecular and Clinical OncologyFebruary 25, 2022Bruna Cristina Borges, Pedro Augusto Do Amaral, Luiz Ricardo Soldi, Victor Luigi Costa Silva, Fernanda Carvalho De Souza, Felipe Andrés Cordeiro Da Luz, Rogério Agenor De Araújo, Marcelo José Barbosa SilvaRT Total RNA was first extracted using the Total SV RNA Isolation System kit (cat. no. Z3100) in a Maxwell® RSC Instrument (Promega Corporation), and complementary DNA (cDNA) was obtained from total RNA using the GoScript™

(582) Proteasomal Processing Immune Escape Mechanisms in Platinum-Treated Advanced Bladder CancerGenes (Basel)February 25, 2022Michael Wessolly, Fabian D. Mairinger, Thomas Herold, Boris Hadaschik, Tibor Szarvas, Henning ReisAX4920 custom, Promega Maxwell, Fitchburg, MA, USA) on a Maxwell RSC device (Maxwell RSC Instrument AS4500, Promega Maxwell) according to the manufacturer’s manual (R29X) with minor adaptions. Proteinase K end

(583) Cyclosporine A Inhibits Viral Infection and Release as Well as Cytokine Production in Lung Cells by Three SARS-CoV-2 VariantsMicrobiology spectrumFebruary 23, 2022Fenizia C, Galbiati S, Vanetti C, Vago R, Clerici M, Tacchetti C, Daniele T.Maxwell RSC Viral Total Nucleic Acid purification kit was used to extract RNA from 250 μL of cell culture supernatants employing the Maxwell RSC Instrument (Promega, Madison, WI, USA). The remaining supernatant was conveniently stored at −80°C for the TCID50

(584) FYCO1 Frameshift Deletion in Wirehaired Pointing Griffon Dogs with Juvenile CataractGenes (Basel)February 11, 2022Gabriela Rudd Garces, Matthias Christen, Robert Loechel, Vidhya Jagannathan, Tosso Leebisolation. Genomic DNA was isolated from 500 µL of EDTA blood with the Maxwell RSC Whole-Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.3. Whole-Genome Sequencing of an Affected Dog An Illumina TruSeq

(585) Quantitative and Functional Assessment of the Influence of Routinely Used Cryopreservation Media on Mononuclear Leukocytes for Medical ResearchInternational Journal of Molecular SciencesFebruary 7, 2022Patrick Haider, Timothy Hoberstorfer, Manuel Salzmann, Michael B. Fischer, Walter S. Speidl, Johann Wojta, Philipp J. HohensinnerGene Expression Analysis RNA from MNC lysates was prepared using Maxwell RSC simplyRNA Tissue kits on a Maxwell RSC Instrument (both Promega, Madison, WI, USA). To create cDNA from the extracted RNA, 10 μL RNA was added to 10 μL

(586) ABHD5 frameshift deletion in Golden Retrievers with ichthyosisG3 Genes|Genomes|GeneticsFebruary 4, 2022Sarah Kiener, Dominique J Wiener, Kaitlin Hopke, Alison B Diesel, Vidhya Jagannathan, Elizabeth A Mauldin, Margret L Casal, Tosso Leebextraction Genomic DNA was extracted from EDTA blood using the Maxwell® RSC Whole Blood DNA with the Maxwell® RSC instrument (Promega, Dübendorf, Switzerland). Linkage analysis and homozygosity mapping Genotype data for 44

(587) DNA damage response at telomeres boosts the transcription of SARS‐CoV‐2 receptor ACE2 during agingEMBO reportsFebruary 3, 2022Sara Sepe et al.ratios. RNA extraction from cultured cells For MEFs, HeLa, and BJ cells, total RNA was extracted using Maxwell® RSC Instrument, with Maxwell® RSC simplyRNA Tissue Kit (AS134, Promega), following manufacturer’s instructions. NanoVueTM

(588) Performance of RT-qPCR detection of SARS-CoV-2 in unextracted nasopharyngeal samples using the Seegene AllplexTM 2019-nCoV protocolJournal of Virological MethodsFebruary 1, 2022Flavia R.O. Barros, Deborah C.A. Leite, Larissa J. GuimarÃ£es, Juliana M.H. Lopes, Marina W. Vasconcelos, Luciane X. Ferreira, Sandrieli GonÃ§alves, Victor G.F. Pastre, Gabriela Pereira, Alex B. Trentin, et al.thawed samples were incubated at 56 °C for 10 min with 330 μL of lysis solution and transferred to the Maxwell® RSC Instrument cartridges for RNA extraction according to manual instructions (Maxwell® 16 Viral Total Nucleic Acid Purification Kit

(589) The Challenge of Long-Term Cultivation of Human Precision-Cut Lung SlicesThe American Journal of PathologyFebruary 1, 2022Eike B. PreuÃŸ, Stephanie Schubert, Christopher Werlein, Helge Stark, Peter Braubach, Anne HÃ¶fer, Edith K.J. Plucinski, Harshit R. Shah, Robert Geffers, Katherina Sewald, et al.RNASeq RNA was isolated using the Maxwell RSC simplyRNA Cells Kit (Promega, Madison, WI) with the Maxwell RSC Instrument as described in the instruction manual. PCLS were homogenized in 200 μL of chilled 1-thioglycerol/homogenization

(590) Role of TGFβ1 and WNT6 in FGF2 and BMP4-driven endothelial differentiation of murine embryonic stem cellsAngiogenesisFebruary 1, 2022Gualandris A, Noghero A, Cora'' D, Astanina E, Arese M, Bussolino F.[25]. Real-time PCR Total RNA was extracted with Maxwell RSC miRNA Tissue Kit and processed with Maxwell RSC Instrument (Promega). RNAs were quantified with DeNovix spectrophotometer (DeNovix, Wilmington) and reverse transcribed

(591) Downregulation of lncRNA PVT1 inhibits proliferation and migration of mesothelioma cells by targeting FOXM1Oncology reportsFebruary 1, 2022Fujii Y, Amatya VJ, Kushitani K, Suzuki R, Kai Y, Kambara T, Takeshima Y.supplied with 5% CO2 for 72 h. RNA was extracted from the cells using Maxwell® RSC simplyRNA Cells Kit and Maxwell® RSC Instrument (both from Promega Corporation) according to the manufacturer's protocols. The extracted RNA was reverse-transcribed

(592) Liraglutide + PYY3-36 Combination Therapy Mimics Effects of Roux-en-Y Bypass on Early NAFLD Whilst Lacking-Behind in Metabolic ImprovementsJournal of Clinical MedicineJanuary 30, 2022Valentin Metzner, Gloria Herzog, Tobias Heckel, Thorsten Bischler, Julia Hasinger, Christoph Otto, Martin Fassnacht, Andreas Geier, Florian Seyfried, Ulrich DischingerMaxwell® RSC simplyRNA Tissue Kit (AS1340; Promega, Fitchburg, WI, USA) and the Maxwell® RSC Instrument (AS4500; Promega). Prior to extraction, tissues were homogenized using QIAGEN Tissue Lyser II (85300; QIAGEN,

(593) Phenotyping and Quantitative Trait Locus Analysis for the Limited Transpiration Trait in an Upper-Mid South Soybean Recombinant Inbred Line Population (“Jackson” × “KS4895”): High Throughput Aquaporin Inhibitor ScreeningFrontiers in Plant ScienceJanuary 20, 2022Sayantan Sarkar, Avat Shekoofa, Angela McClure, Jason D. Gillmanlines “KS4895” and “Jackson” using a Promega AS1600 kit (Promega, Madison, WI, United States) using a Maxwell RSC instrument (Promega). Two genotypes did not germinate and the leaf tissue could not be collected. Genotypes for

(594) Independent DSG4 frameshift variants in cats with hair shaft dystrophyMolecular Genetics and GenomicsJanuary 1, 2022Sarah Kiener, Ana Rostaher, Silvia RÃ¼fenacht, Vidhya Jagannathan, John P. Sundberg, Monika Welle, Tosso LeebS1). Genomic DNA was isolated from ETDA blood samples with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Histopathological examinations Skin biopsies from alopecic skin

(595) PIEZO1 mechanoreceptor activation reduces adipogenesis in perivascular adipose tissue preadipocytesFrontiers in endocrinologyJanuary 1, 2022Rendon CJ, Flood E, Thompson JM, Chirivi M, Watts SW, Contreras GA.well #4 of the Maxwell® RSC Cartridge to eliminate genomic DNA. The RNA was automated extracted in Maxwell® RSC Instrument (Promega, Madison, WI, USA) following the manufacturer protocol. The purity, concentration, and integrity

(596) Long term high glucose exposure induces premature senescence in retinal endothelial cellsFrontiers in physiologyJanuary 1, 2022Bertelli PM, Pedrini E, Hughes D, McDonnell S, Pathak V, Peixoto E, Guduric-Fuchs J, Stitt AW, Medina RJ.control HRMECs. Extraction was performed using the Maxwell RSC simplyRNA Cells Kit (Promega) and a Maxwell RSC instrument (Promega) following the manufacturer’s instructions. Three biological replicates were sent for each

(597) Clinical relevance and prognostic role of preoperative cell-free single-stranded DNA concentrations in colorectal cancer patientsKorean Journal of Clinical OncologyDecember 31, 2021Hyun Soo Song, Dong Hyun Kang, Hyunjung Kim, Tae Sung Ahn, Tae Wan Kim, Moo-Jun Baekanalysis. Isolation of ccfDNA from plasma ccfDNA was isolated from plasma (100 μL) using a Maxwell RSC Instrument (AS4500; Promega, Madison, WI, USA) and a Maxwell RSC ccfDNA Plasma Kit (AS1480, Promega) according to the user

(598) Distribution and Genetic Diversity of Hepatitis E Virus in Wild and Domestic Rabbits in AustraliaPathogensDecember 17, 2021Maria Jenckel, Ina Smith, Tegan King, Peter West, Patrick L. Taggart, Tanja Strive, Robyn N. Hallstabilization solution [26,27]. RNA from tissue samples (liver and bone marrow) was extracted using the Maxwell® RSC instrument (Promega) in combination with the Maxwell® RSC SimplyRNA Tissue Kit (Promega) according to the manufacturer’s

(599) Clinicopathological and Molecular Characteristics of Macroscopically Yellowish-Colored Chromophobe Renal Cell Carcinoma Compared to Non-Yellowish-Colored Chromophobe Renal Cell CarcinomaClin PatholDecember 12, 2021Fumiyoshi Kojima, Ibu Matsuzaki, Naoto Kuroda, Yurina Mikasa, Fidele Y Musangile, Ryuta Iwamoto, Yuichi Takahashi, Akiko Matsubara, Yasuo Kohjimoto, Isao Hara, et al.was extracted from formalin-fixed paraffin-embedded ChRCC or non-neoplastic renal tissue using the Maxwell® RSC Instrument (Promega Corporation, Madison, WI, USA) with the Maxwell RSC RNA FFPE Kit (Promega Corporation, Madison,

(600) Clinicopathological and Molecular Characteristics of Macroscopically Yellowish-Colored Chromophobe Renal Cell Carcinoma Compared to Non-Yellowish-Colored Chromophobe Renal Cell CarcinomaClinical PathologyDecember 12, 2021Fumiyoshi Kojima et al.was extracted from formalin-fixed paraffin-embedded ChRCC or non-neoplastic renal tissue using the Maxwell® RSC Instrument (Promega Corporation, Madison, WI, USA) with the Maxwell RSC RNA FFPE Kit (Promega Corporation, Madison,

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