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Automated DNA/RNA Purification Sample Preparation Workstations
Maxwell RSC Instrument
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Maxwell RSC Instrument
Maxwell RSC Instrument
Maxwell RSC Instrument from Promega
Be the first to write a review!
Citations:
(802)
Figures:
(1)
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Maxwell RSC Instrument
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Description
The Maxwell RSC Instrument is a platform for automated extraction of nucleic acid from a range of sample types. Extraction methods use sample lysis and binding to paramagnetic particles followed by washing steps and elution.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC Instrument
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS4500
Quantity
EA
Processing Time
30 to 60 min (depending on sample type and method)
Capacity
Up to 16
Dimensions
(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV Lamp
Avg. lifetime approximately 6,000 hrs
Molecular Weight
~85kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (802)
(541)
ABHD5 frameshift deletion in Golden Retrievers with ichthyosis
G3 Genes|Genomes|Genetics
February 4, 2022
Sarah Kiener, Dominique J Wiener, Kaitlin Hopke, Alison B Diesel, Vidhya Jagannathan, Elizabeth A Mauldin, Margret L Casal, Tosso Leeb
extraction Genomic DNA was extracted from EDTA blood using the Maxwell® RSC Whole Blood DNA with the
Maxwell® RSC instrument
(
Promega
, Dübendorf, Switzerland). Linkage analysis and homozygosity mapping Genotype data for 44
(542)
DNA damage response at telomeres boosts the transcription of SARS‐CoV‐2 receptor ACE2 during aging
EMBO reports
February 3, 2022
Sara Sepe et al.
ratios. RNA extraction from
cultured cells
For MEFs, HeLa, and BJ cells, total RNA was extracted using
Maxwell® RSC Instrument
, with
Maxwell® RSC simplyRNA Tissue Kit
(AS134,
Promega
), following manufacturer’s instructions. NanoVueTM
(543)
Performance of RT-qPCR detection of SARS-CoV-2 in unextracted nasopharyngeal samples using the Seegene AllplexTM 2019-nCoV protocol
Journal of Virological Methods
February 1, 2022
Flavia R.O. Barros, Deborah C.A. Leite, Larissa J. Guimarães, Juliana M.H. Lopes, Marina W. Vasconcelos, Luciane X. Ferreira, Sandrieli Gonçalves, Victor G.F. Pastre, Gabriela Pereira, Alex B. Trentin,
et al.
thawed samples were incubated at 56 °C for 10 min with 330 μL of lysis solution and transferred to the
Maxwell® RSC Instrument
cartridges for RNA extraction according to manual instructions (
Maxwell® 16 Viral Total Nucleic Acid Purification Kit
(544)
The Challenge of Long-Term Cultivation of Human Precision-Cut Lung Slices
The American Journal of Pathology
February 1, 2022
Eike B. Preuß, Stephanie Schubert, Christopher Werlein, Helge Stark, Peter Braubach, Anne Höfer, Edith K.J. Plucinski, Harshit R. Shah, Robert Geffers, Katherina Sewald,
et al.
RNASeq RNA was isolated using the
Maxwell RSC simplyRNA Cells Kit
(
Promega
, Madison, WI) with the
Maxwell RSC Instrument
as described in the instruction manual. PCLS were homogenized in 200 μL of chilled 1-thioglycerol/homogenization
(545)
Role of TGFβ1 and WNT6 in FGF2 and BMP4-driven endothelial differentiation of murine embryonic stem cells
Angiogenesis
February 1, 2022
Gualandris A, Noghero A, Cora'' D, Astanina E, Arese M, Bussolino F.
[25].
Real-time PCR
Total RNA was extracted with
Maxwell RSC miRNA Tissue Kit
and processed with
Maxwell RSC Instrument
(
Promega
). RNAs were quantified with DeNovix spectrophotometer (DeNovix, Wilmington) and reverse transcribed
(546)
Downregulation of lncRNA PVT1 inhibits proliferation and migration of mesothelioma cells by targeting FOXM1
Oncology reports
February 1, 2022
Fujii Y, Amatya VJ, Kushitani K, Suzuki R, Kai Y, Kambara T, Takeshima Y.
supplied with 5% CO2 for 72 h. RNA was extracted from the cells using
Maxwell® RSC simplyRNA Cells Kit
and
Maxwell® RSC Instrument
(both from
Promega Corporation
) according to the manufacturer's protocols. The extracted RNA was reverse-transcribed
(547)
Liraglutide + PYY3-36 Combination Therapy Mimics Effects of Roux-en-Y Bypass on Early NAFLD Whilst Lacking-Behind in Metabolic Improvements
Journal of Clinical Medicine
January 30, 2022
Valentin Metzner, Gloria Herzog, Tobias Heckel, Thorsten Bischler, Julia Hasinger, Christoph Otto, Martin Fassnacht, Andreas Geier, Florian Seyfried, Ulrich Dischinger
Maxwell® RSC simplyRNA Tissue Kit
(
AS1340
;
Promega
, Fitchburg, WI, USA) and the
Maxwell® RSC Instrument
(
AS4500
;
Promega
). Prior to extraction, tissues were homogenized using QIAGEN Tissue Lyser II (85300; QIAGEN,
(548)
Phenotyping and Quantitative Trait Locus Analysis for the Limited Transpiration Trait in an Upper-Mid South Soybean Recombinant Inbred Line Population (“Jackson” × “KS4895”): High Throughput Aquaporin Inhibitor Screening
Frontiers in Plant Science
January 20, 2022
Sayantan Sarkar, Avat Shekoofa, Angela McClure, Jason D. Gillman
lines “KS4895” and “Jackson” using a
Promega
AS1600
kit (Promega, Madison, WI, United States) using a
Maxwell RSC instrument
(
Promega
). Two genotypes did not germinate and the leaf tissue could not be collected. Genotypes for
(549)
Independent DSG4 frameshift variants in cats with hair shaft dystrophy
Molecular Genetics and Genomics
January 1, 2022
Sarah Kiener, Ana Rostaher, Silvia Rüfenacht, Vidhya Jagannathan, John P. Sundberg, Monika Welle, Tosso Leeb
S1). Genomic DNA was isolated from ETDA blood samples with the Maxwell RSC Whole Blood Kit using a
Maxwell RSC instrument
(
Promega
, Dübendorf, Switzerland). Histopathological examinations Skin
biopsies
from alopecic skin
(550)
PIEZO1 mechanoreceptor activation reduces adipogenesis in perivascular adipose tissue preadipocytes
Frontiers in endocrinology
January 1, 2022
Rendon CJ, Flood E, Thompson JM, Chirivi M, Watts SW, Contreras GA.
well #4 of the Maxwell® RSC Cartridge to eliminate genomic DNA. The RNA was automated extracted in
Maxwell® RSC Instrument
(
Promega
, Madison, WI, USA) following the manufacturer protocol. The purity, concentration, and integrity
(551)
Long term high glucose exposure induces premature senescence in retinal endothelial cells
Frontiers in physiology
January 1, 2022
Bertelli PM, Pedrini E, Hughes D, McDonnell S, Pathak V, Peixoto E, Guduric-Fuchs J, Stitt AW, Medina RJ.
control HRMECs. Extraction was performed using the
Maxwell RSC simplyRNA Cells Kit
(Promega) and a
Maxwell RSC instrument
(
Promega
) following the manufacturer’s instructions. Three biological replicates were sent for each
(552)
Clinical relevance and prognostic role of preoperative cell-free single-stranded DNA concentrations in colorectal cancer patients
Korean Journal of Clinical Oncology
December 31, 2021
Hyun Soo Song, Dong Hyun Kang, Hyunjung Kim, Tae Sung Ahn, Tae Wan Kim, Moo-Jun Baek
analysis. Isolation of ccfDNA from plasma ccfDNA was isolated from plasma (100 μL) using a
Maxwell RSC Instrument
(
AS4500
;
Promega
, Madison, WI, USA) and a
Maxwell RSC ccfDNA Plasma Kit
(
AS1480
,
Promega
) according to the user
(553)
Distribution and Genetic Diversity of Hepatitis E Virus in Wild and Domestic Rabbits in Australia
Pathogens
December 17, 2021
Maria Jenckel, Ina Smith, Tegan King, Peter West, Patrick L. Taggart, Tanja Strive, Robyn N. Hall
stabilization solution [26,27]. RNA from tissue samples (liver and
bone
marrow) was extracted using the
Maxwell® RSC instrument
(
Promega
) in combination with the
Maxwell® RSC SimplyRNA Tissue Kit
(
Promega
) according to the manufacturer’s
(554)
Clinicopathological and Molecular Characteristics of Macroscopically Yellowish-Colored Chromophobe Renal Cell Carcinoma Compared to Non-Yellowish-Colored Chromophobe Renal Cell Carcinoma
Clinical Pathology
December 12, 2021
Fumiyoshi Kojima et al.
was extracted from
formalin-fixed paraffin-embedded
ChRCC or non-neoplastic renal tissue using the
Maxwell® RSC Instrument
(
Promega Corporation
, Madison, WI, USA) with the
Maxwell RSC RNA FFPE Kit
(
Promega Corporation
, Madison,
(555)
Plasma ctDNA is a tumor tissue surrogate and enables clinical-genomic stratification of metastatic bladder cancer
Nature Communications
December 1, 2021
Gillian Vandekerkhove, Jean-Michel Lavoie, Matti Annala, Andrew J. Murtha, Nora Sundahl, Simon Walz, Takeshi Sano, Sinja Taavitsainen, Elie Ritch, Ladan Fazli,
et al.
using the QIAGEN DNeasy Blood and Tissue Kit, or the
Promega
Maxwell® RSC Blood DNA Kit
and
Promega
Maxwell® RSC Instrument
. Extracted gDNA was quantified with a NanoDrop spectrophotometer. For archival FFPE tissue samples,
(556)
DNA methylation profiling identifies two distinct subgroups in breast cancers with low hormone receptor expression, mainly associated with HER2 amplification status
Clinical Epigenetics
December 1, 2021
Philipp Jurmeister, Karsten Weber, Sonia Villegas, Thomas Karn, Michael Untch, Anne Thieme, Volkmar Müller, Eliane Taube, Peter Fasching, Wolfgang D. Schmitt,
et al.
performed to reach a
tumor
cell content of at least 70%. Semi-automated DNA extraction was performed on the
Maxwell RSC Instrument
using the Maxwell RSC FFPE Plus DNA Purification Kit (Custom, AX4920;
Promega
), according to the manufacturer’s
(557)
Alteration of DNA mismatch repair capacity underlying the co-occurrence of non-small-cell lung cancer and nonmedullary thyroid cancer
Scientific Reports
December 1, 2021
Shiro Fujita, Katsuhiro Masago
analysis MSI analyses were performed on genomic DNA extracted using the Maxwell RSC FFPE DNA Kit and
Maxwell RSC Instrument
(
Promega
). MSI analyses were performed using the MSI Analysis System v1.2 (Promega). The analysis included
(558)
Genome-wide DNA methylation profiling is able to identify prefibrotic PMF cases at risk for progression to myelofibrosis
Clinical Epigenetics
December 1, 2021
Ulrich Lehmann, Helge Stark, Stephan Bartels, Jerome Schlue, Guntram Büsche, Hans Kreipe
committee for retrospective analyses. DNA extraction Extraction of genomic DNA was performed using the
Maxwell RSC instrument
(
Promega
, Madison, WI, USA) and the
Maxwell RSC DNA FFPE kit
according to the manufacturer's instructions.
(559)
Identification of molecular signatures associated with early relapse after complete resection of lung adenocarcinomas
Scientific Reports
December 1, 2021
Helen Pasternack et al.
DNA and mRNA were performed using the Maxwell RSC DNA FFPE resp. Maxwell RSC RNA FFPE Kits and the
Maxwell RSC instrument
(
Promega
, Fitchburg WI, U.S.A.). DNA samples were quantified with Nanodrop (ThermoFisher, Waltham MA, U.S.A.).
(560)
UV-C irradiation is highly effective in inactivating SARS-CoV-2 replication
Scientific Reports
December 1, 2021
Mara Biasin et al.
days post
infection
. RNA was extracted from VeroE6
cell culture
supernatant and cell lysate by the
Maxwell RSC Instrument
with
Maxwell RSC Viral Total Nucleic Acid Purification Kit
(
Promega
, Fitchburg, WI, USA), quantified
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Maxwell RSC Instrument from Promega
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