Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(541) Epidemiologic and Genomic Analysis of the Severe Acute Respiratory Syndrome Coronavirus 2 Epidemic in the Nebraska Region of the United States, March 2020â€“2021Frontiers in MicrobiologyMay 18, 2022Jacob A. Siedlik, Cynthia J. Watson, Morgan A. Raine, Anne V. Cheng, Richard V. Goering, Holly A. F. Stessman, Michael Belshanstored at −80°C until used. Viral RNA (vRNA) was isolated from 250 μl of nasal swab samples using a Maxwell® RSC Instrument (Promega, Madison, WI, United States) in the Creighton University Biorepository and Tissue Processing

(542) The Lack of Alternative Oxidase 1a Restricts in vivo Respiratory Activity and Stress-Related Metabolism for Leaf Osmoprotection and Redox Balancing Under Sudden Acute Water and Salt Stress in Arabidopsis thalianaFrontiers in Plant ScienceMay 17, 2022NÃ©stor F. Del-Saz, Ariadna Iglesias-Sanchez, David Alonso-Forn, Miguel LÃ³pez-GÃ³mez, Francisco Palma, MarÃa JosÃ© Clemente-Moreno, Alisdair R. Fernie, Miquel Ribas-Carbo, Igor Florez-Sarasaleaves by using Maxwell® RSC Plant RNA Kit (Promega Biotech Ibérica, Madrid, Spain) and automated system Maxwell® RSC Instrument (Promega Biotech Ibérica, Madrid, Spain) according to the manufacturer’s instructions, and was quantified

(543) Molecular Analysis of SARS-CoV-2 Lineages in ArmeniaVirusesMay 17, 2022Diana Avetyan, Siras Hakobyan, Maria Nikoghosyan, Lilit Ghukasyan, Gisane Khachatryan, Tamara Sirunyan, Nelli Muradyan, Roksana Zakharyan, Andranik Chavushyan, Varduhi Hayrapetyan, et al.samples was 194. 2.2. Real-Time PCR Detection of SARS-CoV-2 Automated RNA isolation was performed with Maxwell RSC Instrument using Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega Corporation Inc, Fitchburg, WI,

(544) Emergence of SARS-CoV-2 Alpha lineage and its correlation with quantitative wastewater-based epidemiology dataWater ResearchMay 15, 2022Elena Radu et al.each sample were used for further RNA extraction. The RNA extraction was performed on Maxwell® RSC AS4500 (Promega) device using Viral RNA/DNA Concentration and Extraction Kit from Wastewater (Promega). The

(545) Liquid Biopsy in Colorectal Cancer: Quo Vadis? Implementation of Liquid Biopsies in Routine Clinical Patient Care in Two German Comprehensive Cancer CentersFrontiers in OncologyMay 12, 2022Laura E. Fischer, Sebastian Stintzing, Volker Heinemann, Ulrich Keilholz, Dietmar Keune, Claudia Vollbrecht, Thomas Burmeister, Andreas Kind, Lena Weiss, David Horst, et al.centrifuged plasma (10 minutes, 2000 g, RT; 10 minutes, 3000 g, RT) using magnetic bead based semi-automated Maxwell RSC instrument (Promega, Madison, WI, U.S.A.) after proteinase digestions (5% 20mg/ml Proteinase K (Promega) per milliliter

(546) Identification of Potential mRNA Biomarkers in Milk Small Extracellular Vesicles of Enzootic Bovine Leukosis CattleVirusesMay 11, 2022Mami Hiraoka, Shigeo Takashima, Yoshiko Wakihara, Yuji O. Kamatari, Kaori Shimizu, Ayaka Okada, Yasuo Inoshimadescribed previously [29], with slight modifications. mRNA in sEVs was extracted using Maxwell RSC (AS4500, Promega). Before microarray analysis, the quality and concentration of the extracted mRNAs was determined

(547) Genomic Comparison of Eight Closed Genomes of Multidrug-Resistant Salmonella enterica Strains Isolated From Broiler Farms and Processing Plants in Trinidad and TobagoFrontiers in MicrobiologyMay 10, 2022Meghan Maguire, Anisa S. Khan, Abiodun A. Adesiyun, Karla Georges, Narjol Gonzalez-Escalonadata. Nucleic Acid Extraction DNA was extracted using the Maxwell RSC cultured cells DNA kit with a Maxwell RSC instrument (Promega, Madison, WI, USA) following the manufacturer's protocols for Gram-negative bacteria with additional

(548) Molecular signature of postmortem lung tissue from COVID-19 patients suggests distinct trajectories driving mortalityDisease Models & MechanismsMay 1, 2022Anshul Budhraja, Anubhav Basu, Atish Gheware, Dasari Abhilash, Seesandra Rajagopala, Suman Pakala, Madhuresh Sumit, Animesh Ray, Arulselvi Subramaniam, Purva Mathur, et al.extraction, library preparation and sequencing Total RNA was extracted from lung tissue using the Maxwell® RSC Instrument and the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega). The concentration of RNA and

(549) Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1Mucosal immunologyMay 1, 2022Stölting H, Baillon L, Frise R, Bonner K, Hewitt RJ, Molyneaux PL, Gore ML, Breathing Together Consortium, Barclay WS, Saglani S, et al.extracted from two Transwell inserts per condition using the Maxwell® RSC simplyRNA Blood kit on the Maxwell® RSC instrument (both Promega) and transcribed into cDNA using the High-Capacity cDNA Reverse Transcription kit (Applied

(550) Setting Up an Ultra-Fast Next-Generation Sequencing Approach as Reflex Testing at Diagnosis of Non-Squamous Non-Small Cell Lung Cancer; Experience of a Single Center (LPCE, Nice, France)Cancers (Basel)April 30, 2022Marius Ilié et al.Next-Generation Sequencing Briefly, nucleic acids were either extracted using the Maxwell RSC Instrument (Promega, catalog number AS4500) with the Maxwell RSC FFPE Plus DNA kit (catalog number AS1720) and Maxwell RSC RNA FFPE kit (catalog

(551) Independent COL5A1 Variants in Cats with Ehlers-Danlos SyndromeGenes (Basel)April 29, 2022Sarah Kiener, Neoklis Apostolopoulos, Jennifer Schissler, Pascal-Kolja Hass, Fabienne Leuthard, Vidhya Jagannathan, Carole Schuppisser, Sara Soto, Monika Welle, Ursula Mayer, et al.Extraction Genomic DNA was isolated from EDTA blood using the Maxwell® RSC Whole Blood DNA Kit with the Maxwell® RSC instrument (Promega, Dübendorf, Switzerland). 2.5. Whole-Genome Sequencing and Variant Calling Illumina TruSeq

(552) The Link Between 15-F2t-Isoprostane Activity and Acute Bovine Endothelial Inflammation Remains ElusiveFrontiers in Veterinary ScienceApril 29, 2022Ashley K. Putman, Lorraine M. Sordillo, G. Andres Contreras−20°C pending RNA extraction within 1 mo of collection. Extraction of RNA occurred utilizing a Promega Maxwell RSC Instrument following the manufacturer's protocol. Quantification and the quality of RNA was assessed with a Nanodrop

(553) SWAN pathway-network identification of common aneuploidy-based oncogenic driversNucleic Acids ResearchApril 22, 2022Robert R Bowers, Christian M Jones, Edwin A Paz, John K Barrows, Kent E Armeson, David T Long, Joe R Delaneyplate. Whole-exome sequencing and data processing Samples were processed using a Promega Maxwell RSC Instrument (AS4500) and Maxwell RSC Tissue DNA kit (AS1610) to obtain purified DNA. DNA was sent to GENEWIZ for whole-exome

(554) The Clinically Actionable Molecular Profile of Early versus Late-Stage Non-Small Cell Lung Cancer, an Individual Age and Sex Propensity-Matched Pair AnalysisCurrent OncologyApril 11, 2022Anna L. McGuire, Melissa K. McConechy, Barb L. Melosky, John C. English, James J. Choi, Defen Peng, John Yee, Benjamin L. S. Furman, Rosalia Aguirre Hernandez, Pedro Feijao, et al.the manufacturer’s instructions. RNA was extracted using the Promega RNA FFPE kit using the Promega Maxwell RSC instrument. The Canexia Health Find ItTM assay, a FPPE solid tumor DNA-based assay, and the RNA-based Fusions assay

(555) A multi‐tissue view on telomere dynamics and postnatal growthJournal of Experimental Zoology Part A: Ecological and Integrative PhysiologyApril 1, 2022Sarah E. Wolf, Kimberly A. RosvallCriscuolo et al., 2009). We extracted DNA from blood, brain, liver, and adrenals. We used the automated Maxwell® RSC Instrument (Promega, Madison, WI) and Whole Blood DNA Kit (Promega no. AS1520) to extract DNA from ≤25μL whole

(556) Gentamicin at sub-inhibitory concentrations selects for antibiotic resistance in the environmentISME CommunicationsMarch 30, 2022Concepcion Sanchez-Cid, Alexandre Guironnet, Christoph Keuschnig, Laure Wiest, Emmanuelle Vulliet, Timothy M. Vogel1 min, 300 µl of supernatant were added to 300 µl of Lysis Buffer (Promega) and purified using the Maxwell® RSC Instrument and the Maxwell® RSC PureFood GMO and Authentication Kit (Promega). DNA was eluted in 100 µl of Elution Buffer

(557) Effect of differences in light source environment on transcriptome of leaf lettuce (Lactuca sativa L.) to optimize cultivation conditionsPLOS ONEMarch 29, 2022Soichiro Nagano et al.separate tube. Total RNA was extracted using a Maxwell RSC Plant RNA Kit (Promega, Madison, WI, USA) and a Maxwell RSC Instrument (Promega). A tube containing total RNA and PrimeScript RT reagent Kit (Takara Bio) was heated in a block

(558) 15-F2t-Isoprostane Favors an Anti-Inflammatory Phenotype in RAW 264.7 Macrophages during Endotoxin ChallengeAntioxidants (Basel)March 19, 2022Ashley K. Putman, G. Andres Contreras°C pending RNA extraction within 1 mo of collection. Extraction of RNA occurred utilizing a Promega Maxwell RSC Instrument following the manufacturer’s protocol. Quantification and the quality of RNA was assessed with the Nucleid

(559) RNA-Seq Analyses Reveal Roles of the HVCN1 Proton Channel in Cardiac pH HomeostasisFrontiers in Cell and Developmental BiologyMarch 16, 2022Xin Wu, Yawei Li, Mark Maienschein-Cline, Leonid Feferman, Longjun Wu, Liang HongMaxwell® RSC simplyRNA Cells Kit (Promega AS1390) based on manufacturer’s instructions, using Maxwell® RSC Instrument (Promega AS4500), and then RNA was quantified using Qubit 4.0 Fluorometer (Invitrogen) with the Qubit RNA HS Assay Kit

(560) Paper-based immunoassay based on 96-well wax-printed paper plate combined with magnetic beads and colorimetric smartphone-assisted measure for reliable detection of SARS-CoV-2 in salivaBiosensors and BioelectronicsMarch 15, 2022Laura Fabiani, Vincenzo Mazzaracchio, Danila Moscone, Silvia Fillo, Riccardo De Santis, Anella Monte, Donatella Amatore, Florigio Lista, Fabiana Arduiniextracted from 300 μL of swab medium using a Maxwell RSC Viral Total Nucleic Acid Purification Kit on Maxwell RSC Instrument (Promega, USA). Total nucleic acid was eluted in a final volume of 50 μL of nuclease-free water. Then,

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