Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(461) Community-Based SARS-CoV-2 Testing Using Saliva or Nasopharyngeal Swabs to Compare the Performance of Weekly COVID-19 Screening to Wastewater SARS-CoV-2 SignalsACS ES&T WaterOctober 14, 2022Zhengchun Lu, Amanda E. Brunton, Maedeh Mohebnasab, Anthony Deloney, Kenneth J. Williamson, Blythe A. Layton, Scott Mansell, Alice Brawley-Chesworth, Peter Abrams, Kimberly A. Wilcox, et al.previously described.23 Briefly, RNA was extracted using one of the three different automated machines: Maxwell RSC Instrument (Promega, Madison, MI), MagNA Pure 96 System (Roche Diagnostics, Pleasanton, CA), and KingFisher Flex

(462) Genetic variants for prediction of gestational diabetes mellitus and modulation of susceptibility by a nutritional intervention based on a Mediterranean dietFront Endocrinol (Lausanne)October 13, 2022Ana Ramos-Levi, Ana Barabash, Johanna Valerio, Nuria GarcÃa de la Torre, Leire Mendizabal, Mirella Zulueta, Maria Paz de Miguel, Angel Diaz, Alejandra Duran, Cristina Familiar, et al.analysis Genomic DNA was extracted from EDTA-stabilized blood samples taken during the OGTT using the Maxwell RSC instrument (Promega, Dubendorf, Switzerland). Genotyping was performed by IPLEX MassARRAY PCR using the Agena platform

(463) ACADM Frameshift Variant in Cavalier King Charles Spaniels with Medium-Chain Acyl-CoA Dehydrogenase DeficiencyGenes (Basel)October 13, 2022Matthias Christen, Jos Bongers, DÃ©borah Mathis, Vidhya Jagannathan, Rodrigo Gutierrez Quintana, Tosso LeebDNA Extraction Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). In addition to the affected dog, 162 blood samples from CKCS, which

(464) Interleukinâ€4 receptor alpha signaling regulates monocyte homeostasisThe FASEB JournalOctober 1, 2022Patrick Haider, Julia B. Kralâ€Pointner, Manuel Salzmann, Florian Moik, Sonja Bleichert, Waltraud C. Schrottmaier, Christoph Kaun, Mira Brekalo, Michael B. Fischer, Walter S. Speidl, et al.Quantitative PCR RNA from cell culture lysates was prepared using Maxwell RSC simplyRNA Tissue kits on a Maxwell RSC Instrument (both Promega, Madison, WI, USA). cDNA was created using a 1:1 Oligo(dT)/Random Primer Reverse Transcriptase

(465) Physiological intron retaining transcripts in the cytoplasm abound during human motor neurogenesisGenome ResearchOctober 1, 2022Marija Petrić Howe, Hamish Crerar, Jacob Neeves, Jasmine Harley, Giulia E. Tyzack, Pierre Klein, Andres Ramos, Rickie Patani, Raphaëlle Luisierclustering. RNA extraction RNA was extracted using the Maxwell RSC simplyCells RNA kit (Promega) and the Maxwell RSC instrument (Promega), according to the manufacturer's instructions. Contaminating genomic DNA was removed by DNase

(466) Pet Reptiles in Poland as a Potential Source of Transmission of SalmonellaPathogensSeptember 29, 2022Marta Dec, Magdalena ZajÄ…c, Andrzej Puchalski, Klaudiusz Szczepaniak, Renata Urban-ChmielCultured Cells (AS1620 Promega, Madison, WI, USA) according to the manufacturer’s instructions with the Maxwell® RSC Instrument (Promega, Madison, WI, USA). For yield and purity checks, all samples were measured with NanoDropTM

(467) Development, Evaluation, and Implementation of a House-Made Targeted Next-Generation Sequencing Spoligotyping in a French LaboratoryInternational Journal of Molecular SciencesSeptember 25, 2022Charlotte Genestet, Yannick Baffert, Maxime VallÃ©e, Albin Bernard, Yvonne Benito, GÃ©rard Lina, Elisabeth Hodille, Oana Dumitrescu, Lyon TB Study GroupSilico WGS-Based Spoligotyping For MTBC WGS, genomic DNA was purified from cleared lysates using the Maxwell RSC Instrument (Promega, Madison, WI, USA) automated DNA extraction system and the Maxwell RSC Blood DNA Kit (Promega).

(468) High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistanceNature communicationsSeptember 22, 2022Cortés-López M, Schulz L, Enculescu M, Paret C, Spiekermann B, Quesnel-Vallières M, Torres-Diz M, Unic S, Busch A, Orekhova A, et al.were acquired with GBox instrument (Syngene). Quantitative real-time PCR RNA was extracted using the Maxwell RSC Instrument (Promega) and the Maxwell® RSC simplyRNA Cells Kit (AS1390, Promega). 0.5 µg or RNA were reverse-transcribed

(469) Polymorphism of Drug Transporters, Rather Than Metabolizing Enzymes, Conditions the Pharmacokinetics of RasagilinePharmaceuticsSeptember 21, 2022Pablo Zubiaur, Miriam Matas, Samuel MartÃn-VÃlchez, Paula Soria-Chacartegui, Gonzalo Villapalos-GarcÃa, Laura Figueiredo-Tor, SofÃa Calleja, Marcos Navares-GÃ³mez, Alejandro de Miguel, JesÃºs Novalbos, et al.reconstituted with 0.9% sodium chloride solution and 500 µL were used for DNA extraction with an automated Maxwell® RSC instrument (Promega, Waltham, MA, USA). A Qubit® 3.0 fluorometer (ThermoFisher, Waltham, MA, USA) was used to measure

(470) Detection of BRCA1/2 pathogenic variants in patients with breast and/or ovarian cancer and their families. Analysis of 3,458 cases from Lower Silesia (Poland) according to the diagnostic algorithm of the National Cancer Control ProgrammeFrontiers in GeneticsSeptember 12, 2022Anna Doraczynska-Kowalik et al.Maxwell RSC Blood DNA Kit (Promega Corporation) according to the manufacturer’s protocol on a Maxwell RSC instrument (AS4500, Promega Corporation). After extraction, the DNA samples were quantified using the fluorometric method,

(471) Integrated Antitumor Activities of Cellular Immunotherapy with CIK Lymphocytes and Interferons against KIT/PDGFRA Wild Type GISTInternational journal of molecular sciencesSeptember 8, 2022Fiorino E, Merlini A, D'Ambrosio L, Cerviere I, Berrino E, Marchiò C, Giraudo L, Basiricò M, Massa A, Donini C, et al.Analysis Total RNA automatic extraction of wtGISTc was performed using the Maxwell RSC miRNA Tissue KIT and Maxwell RSC Instrument (Promega, Milan, Italy), according to the manufacturer’s instructions. The RNA was reverse-transcribed

(472) CYP2C8 *3 and *4 define CYP2C8 phenotype: An approach with the substrate cinitaprideClinical and Translational ScienceSeptember 6, 2022Diana MarÃa CampodÃ³nico, Pablo Zubiaur, Paula Soriaâ€Chacartegui, Ana CasajÃºs, Gonzalo Villapalosâ€GarcÃa, Marcos Navaresâ€GÃ³mez, AntÃa GÃ³mezâ€FernÃ¡ndez, RaÃºl Parraâ€GarcÃ©s, Gina MejÃaâ€Abril, Manuel RomÃ¡n, et al.phenotyping DNA was extracted from 500 μl of thawed peripheral blood stored in EDTA‐K2 tubes in a Maxwell® RSC Instrument (Promega Biotech Ibérica S.L.). Genotyping was performed by real‐time quantitative polymerase chain reaction

(473) Prospective evaluation of the role of imaging techniques and TMPRSS2:ERG mutation for the diagnosis of clinically significant prostate cancerFrontiers in OncologySeptember 6, 2022Massimo Lazzeri, Vittorio Fasulo, Giovanni Lughezzani, Alessio Benetti, Giulia SoldÃ , Rosanna Asselta, Ilaria De Simone, Marco Paciotti, Pier Paolo Avolio, Roberto Contieri, et al.withdrawal. RNA was extracted using the Maxwell RSC miRNA Tissue Kit (Promega, Madison, WI, USA) on a Maxwell RSC instrument (Promega). Random primers (Promega) and the Superscript-IV Reverse Transcriptase (Thermo Fisher Scientific,

(474) Development of a prediction protocol for the screening of metabolic associated fatty liver disease in children with overweight or obesityPediatric ObesitySeptember 1, 2022Maddi Oses, Cristina Cadenasâ€Sanchez, MarÃa Medrano, Arkaitz Galbete, Emiliano Mirandaâ€Ferrua, Jonatan R. Ruiz, Felix SÃ¡nchezâ€Valverde, Francisco B. Ortega, Rafael Cabeza, Arantxa Villanueva, et al.calculated. 32 Genomic DNA was extracted from white blood cells using Maxwell® RSC Blood DNA Kit and Maxwell® RSC Instrument (Promega) equipment. The genotyping was done by an Illumina system (Illumina, Inc, San Diego, CA, USA)

(475) Gut dysbiosis in cutaneous Tâ€cell lymphoma is characterized by shifts in relative abundances of specific bacterial taxa and decreased diversity in more advanced diseaseJournal of the European Academy of Dermatology and VenereologySeptember 1, 2022M.J. Hooper, T.M. LeWitt, Y. Pang, F.L. Veon, G.E. Chlipala, L. Feferman, S.J. Green, D. Sweeney, K.T. Bagnowski, M.B. Burns, et al.Genomic DNA was extracted with a Maxwell® RSC Fecal Microbiome DNA Kit (Promega; Madison, WI, USA) on a Maxwell® RSC Instrument, following the manufacturer's protocol. 16S rRNA amplification and sequencing Genomic DNA was prepared

(476) Acute lymphoblastic leukemia necessitates GSH-dependent ferroptosis defenses to overcome FSP1-epigenetic silencingRedox biologySeptember 1, 2022Pontel LB, Bueno-Costa A, Morellato AE, Carvalho Santos J, Roué G, Esteller M.cell pellets using the SimplyRNA kit (Promega #AS1340) in the automated Maxwell RSC device (Promega, #AS4500). 2 μg of total RNA were converted to cDNA using the RevertAid First Strand cDNA synthesis kit (Thermofisher Scientific

(477) SARS‐CoV‐2 surveillance in Norway rats ( Rattus norvegicus ) from Antwerp sewer system, BelgiumTransboundary and Emerging DiseasesSeptember 1, 2022Valeria Carolina Colombo et al.concentrate with the automated Maxwell PureFood GMO and the Authentication RNA extraction kit in the Maxwell® RSC Instrument (Promega). Reverse transcription and amplification of the N1, N2 and E genes was performed by qPCR in

(478) Gut Microbiome and Mycobiome Alterations in an In Vivo Model of Alzheimerâ€™s DiseaseGenes (Basel)August 31, 2022Valeria Dâ€™Argenio, Iolanda Veneruso, Chunmei Gong, Valentina Cecarini, Laura Bonfili, Anna Maria Eleuteriprocessing. Genomic DNA was extracted from all stool samples by using the RSC Blood DNA kit and the Maxwell RSC instrument (both from Promega, Madison, WI, USA), as previously reported [19]. Specifically, 100 mg of each stool

(479) DNA damage response and repair genes in advanced bone and soft tissue sarcomas: An 8-gene signature as a candidate predictive biomarker of response to trabectedin and olaparib combinationFrontiers in OncologyAugust 30, 2022Alessandra Merlini, Maria Laura Centomo, Giulio Ferrero, Giulia Chiabotto, Umberto Miglio, Enrico Berrino, Giorgia Giordano, Silvia Brusco, Alberto Pisacane, Elena Maldi, et al.extracted from FFPE samples with Maxwell® RSC FFPE Kit (Promega Corporation, Madison, WI, USA) and Maxwell® RSC Instrument. RNA purity, concentration and fragmentation were determined using DeNovix DS-11+ Spectrophotometer

(480) Colorectal Adenocarcinomas Harboring ALK Fusion Genes: A Clinicopathologic and Molecular Genetic Study of 12 Cases and Review of the LiteratureAmerican Journal of Surgical PathologyAugust 29, 2022Jerzy Lasota et al.next-generation sequencing were previously reported.24 Briefly, nucleic acids were recovered from FFPE tumor samples using a Maxwell® RSC instrument (Promega, Madison, WI). Target-specific RNA libraries for next-generation sequencing (NGS) were constructed

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