Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(461) Increased HRD score in cisplatin resistant penile cancer cellsBMC CancerDecember 23, 2022Ria Winkelmann, Katrin Bankov, Claudia Döring, Jaroslav Cinatl, Sebastian Grothe, Florian Rothweiler, Martin Michaelis, Christina Schmitt, Peter J. Wild, Melanie Demes, et al.taken as whole slides. The purification of DNA and RNA from FFPE tissue samples was performed using the Maxwell® RSC Instrument (Promega Corporation, Madison, Wisconsin, USA) with the Maxwell® RSC FFPE Plus RNA Kit (Promega Corporation,

(462) Locally Performed HRD Testing for Ovarian Cancer? Yes, We Can!Cancers (Basel)December 21, 2022Gilda Magliacane, Emanuela Brunetto, Silvia Calzavara, Alice Bergamini, Giovanni Battista Pipitone, Giovanna Marra, Miriam Redegalli, Greta Grassini, Emanuela Rabaiotti, Gianluca Taccagni, et al.percentage of tumor cells above 50%. DNA extraction was performed using thee Maxwell® RSC DNA FFPE kit and Maxwell RSC Instrument (Promega, Milan, Italy) and quantified using the Qubit DNA HS Assay Kit on Qubit 3.0 Fluorometer (ThermoFisher Scientific

(463) Clonal transcriptomics identifies mechanisms of chemoresistance and empowers rational design of combination therapiesElifeDecember 16, 2022Sophia A Wild, Ian G Cannell, Ashley Nicholls, Katarzyna Kania, Dario Bressan, CRUK IMAXT Grand Challenge Team, Gregory J Hannon, Kirsty Sawicka96-well plates were pooled and gDNA extraction of each pooled row of cells was carried out using the Maxwell RSC instrument (Promega) and the ‘Cultured cells DNA extraction kit’ (Promega). These samples were then subjected to

(464) Clonal transcriptomics identifies mechanisms of chemoresistance and empowers rational design of combination therapieseLifeDecember 16, 2022Sophia A Wild, Ian G Cannell, Ashley Nicholls, Katarzyna Kania, Dario Bressan, Gregory J CRUK IMAXT Grand Challenge Team, Gregory J Sawicka, Kirsty CRUK IMAXT Grand Challenge Team96-well plates were pooled and gDNA extraction of each pooled row of cells was carried out using the Maxwell RSC instrument (Promega) and the ‘Cultured cells DNA extraction kit’ (Promega). These samples were then subjected to

(465) Non-uniform dystrophin re-expression after CRISPR-mediated exon excision in the dystrophin/utrophin double-knockout mouse model of DMDMolecular Therapy - Nucleic AcidsDecember 13, 2022Britt Hanson, Sofia Stenler, Nina Ahlskog, Katarzyna Chwalenia, Nenad Svrzikapa, Anna M.L. Coenen-Stass, Marc S. Weinberg, Matthew J.A. Wood, Thomas C. RobertsTotal RNA was isolated from muscle tissues (∼100 μm sections, or one-sixth of the diaphragm) using a Maxwell RSC Instrument and Maxwell RSC simplyRNA Tissue Kit (both Promega, Southampton, UK), and cDNA was reverse-transcribed

(466) RNA-Seq comparative study reveals molecular effectors linked to the resistance of Pinna nobilis to Haplosporidium pinnae parasiteScientific ReportsDecember 8, 2022Pauline Salis, Claire Peyran, Titouan Morage, Simon de Bernard, Julien Nourikyan, Stéphane Coupé, Robert Bunet, Serge Planes15,260,488). The RNA was automatically extracted using Maxwell® RSC Instrument (Promega, reference: AS4500) and Maxwell® RSC simplyRNA Tissue Kit (Promega-reference: AS1340). RNA‐Seq libraries were generated

(467) Loss of primary cilia promotes inflammation and carcinogenesisEMBO reportsDecember 6, 2022Conception Paul, Ruizhi Tang, Ciro Longobardi, Rossano Lattanzio, Thibaut Eguether, Hulya Turali, Julie Bremond, Chloé Maurizy, Monica Gabola, Sophie Poupeau, et al.inflammatory conditioned medium, as described above. Total RNA was extracted from each sample using the Maxwell® RSC Instrument (Promega, Madison, WI, USA) and Maxwell RSC simplyRNA Blood Kit (AS1380, Promega). High‐quality total

(468) Utility of ultra-rapid real-time PCR for detection and prevalence of Rickettsia spp. in ticksBMC Veterinary ResearchDecember 1, 2022A-Tai Truong, Bo-Ram Yun, Mi-Sun Yoo, Jiyeon Lim, Subin Min, Soon-Seek Yoon, Young-Min Yun, Jong-Taek Kim, Yun Sang Choacid was extracted using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit on the automated Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Finally, 50 μl of total nucleic

(469) Physical separation of haplotypes in dikaryons allows benchmarking of phasing accuracy in Nanopore and HiFi assemblies with Hi-C dataGenome BiologyDecember 1, 2022Hongyu Duan, Ashley W. Jones, Tim Hewitt, Amy Mackenzie, Yiheng Hu, Anna Sharp, David Lewis, Rohit Mago, Narayana M. Upadhyaya, John P. Rathjen, et al.and 9 days post inoculation (dpi) was extracted using the Promega Maxwell® RSC Plant RNA Kit with a Maxwell® RSC instrument (Promega.com.au). The spores were induced for germination by placing on the surface of milli-Q water,

(470) Detecting structural variations with precise breakpoints using low-depth WGS data from a single oxford nanopore MinION flowcellScientific ReportsDecember 1, 2022Henry C. M. Leung, Huijing Yu, Yifan Zhang, Wing Sze Leung, Ivan F. M. Lo, Ho Ming Luk, Wai-Chun Law, Ka Kui Ma, Chak Lim Wong, Yat Sing Wong, et al.the real patient data, DNA was extracted from EDTA blood using Maxwell RSC Whole Blood DNA Kit with Maxwell RSC Instrument (Promega, Spain) and stored at − 80 °C upon sequencing. All DNA samples were prepared using an in-house

(471) Toxoplasma gondii and Rickettsia spp. in ticks collected from migratory birds in the Republic of KoreaScientific ReportsDecember 1, 2022A.-Tai Truong, Mi-Sun Yoo, Subin Min, Ji-Yeon Lim, Hyun-Ji Seo, Heung-Chul Kim, Sung-Tae Chong, Terry A. Klein, Chang-uk Park, Sook-Young Cho, et al.extraction using Maxwell® RSC Viral Total Nucleic Acid Purification Kits (Promega, USA) and an automated Maxwell RSC Instrument (Promega). The procedure of isolation was done according to the manufacturer’s instructions. Extracted

(472) Molecular cytogenetic characterization of partial trisomy of the long arm of chromosome 11 in a patient with multiple congenital anomaliesMolecular CytogeneticsDecember 1, 2022Austin Walker, Xianfu Wang, Young Mi Kim, Xianglan Lu, Ashley Taylor, Danielle Demarzo, Shibo Li, Hui Pang(ISCN) 2016. Chromosomal microarray analysis DNA samples were extracted from peripheral blood using Maxwell RSC Instrument and Maxwell RSC Blood DNA Kit (Promega, 2800 Woods Hollow Road, Madison, WI 53711 USA) following manufacturer’s

(473) Rapid detection of single nucleotide polymorphisms using the MinION nanopore sequencer: a feasibility study for perioperative precision medicineJA Clinical ReportsDecember 1, 2022Yoshiteru Tabata, Yoshiyuki Matsuo, Yosuke Fujii, Atsufumi Ohta, Kiichi Hirota(AS1400, Promega, Madison, WI, USA) on the Maxwell RSC automated nucleic acid purification platform (AS4500, Promega). Briefly, the sample (0.3 mL) was mixed with lysis buffer (0.3 mL) and proteinase K solution

(474) The basis and design for time‐restricted eating compared with daily calorie restriction for weight loss and colorectal cancer risk reduction trial ( TRE‐CRC trial)Obesity (Silver Spring)December 1, 2022Kelsey Gabel, Marian L. Fitzgibbon, Cemal Yazici, Peter Gann, Maria Sverdlov, Grace Guzman, Zhengjia Chen, Andrew McLeod, Alyshia Hamm, Krista A. Varady, et al.gene expression Total RNA will be extracted from tissue using the using Maxwell RSC simplyRNA Tissue Kit with the Maxwell RSC Instrument (Promega). Prior to gene expression analysis, RNA quality and quantity will be determined using TapeStation4200

(475) Limited movement of an avian hybrid zone in relation to regional variation in magnitude of climate changeMolecular EcologyDecember 1, 2022Alana Alexander, Mark B. Robbins, Jesse Holmes, Robert G. Moyle, A. Townsend Petersonextraction DNA was extracted from ~15 mg of tissue using a Blood DNA kit and manufacturer protocols on a Maxwell RSC instrument (Promega), with the following modifications: before loading into the cartridge, samples were lysed for

(476) Utility of plasma circulating tumor DNA and tumor DNA profiles in head and neck squamous cell carcinomaScientific ReportsDecember 1, 2022Nobuyuki Chikuie, Yuji Urabe, Tsutomu Ueda, Takao Hamamoto, Takayuki Taruya, Takashi Kono, Kohei Yumii, Sachio TakenoMaxwell RSC Blood DNA Kit (Promega)38. Nucleic acids were automatically extracted from both samples using the Maxwell RSC Instrument. DNA concentrations were measured using a Qubit dsDNAHS Assay Kit (Thermo Fisher Scientific, Waltham,

(477) Mitochondrial fission factor (MFF) frameshift variant in Bullmastiffs with mitochondrial fission encephalopathyAnimal GeneticsDecember 1, 2022Matthias Christen, Rodrigo Gutierrez‐Quintana, Helene Vandenberghe, Adriana Kaczmarska, Jacques Penderis, Roberto José‐López, Angie Rupp, Ian R. Griffiths, Vidhya Jagannathan, Tosso Leebcases. DNA extraction Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega). The same instrument was used for DNA isolation from FFPE tissue samples with the Maxwell RSC DNA FFPE kit

(478) ECNano: A cost-effective workflow for target enrichment sequencing and accurate variant calling on 4800 clinically significant genes using a single MinION flowcellBMC Medical GenomicsDecember 1, 2022Amy Wing-Sze Leung, Henry Chi-Ming Leung, Chak-Lim Wong, Zhen-Xian Zheng, Wui-Wang Lui, Ho-Ming Luk, Ivan Fai-Man Lo, Ruibang Luo, Tak-Wah Lamwere extracted from EDTA blood samples. The automated DNA extraction was performed using the Promega Maxwell® RSC Instrument (Promega, Madison, WA, USA) with the Promega Maxwell® RSC Blood DNA Kit. The pure standard DNA sample

(479) Droplet digital PCR-based analyses for robust, rapid, and sensitive molecular diagnostics of gliomasActa Neuropathologica CommunicationsDecember 1, 2022Marietta Wolter, Jörg Felsberg, Bastian Malzkorn, Kerstin Kaulich, Guido ReifenbergerGermany), the GeneRead DNA FFPE Kit (Qiagen), or the Maxwell® RSC FFPE Plus DNA Kit together with the Maxwell® RSC instrument (Promega, Mannheim, Germany). DNA extraction from frozen tissue samples was performed with the PureLink™ Genomic DNA Mini Kit

(480) DNA methylation-based classification of sinonasal tumorsNature CommunicationsNovember 28, 2022Philipp Jurmeister, Stefanie Glöß, Renée Roller, Maximilian Leitheiser, Simone Schmid, Liliana H. Mochmann, Emma Payá Capilla, Rebecca Fritz, Carsten Dittmayer, Corinna Friedrich, et al.light microscopy of hematoxylin and eosin-stained sections. Semi-automated DNA extraction was performed on the Maxwell RSC Instrument using the Maxwell RSC FFPE Plus DNA Purification Kit (Custom, AX4920; Promega). Extracted total DNA

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