Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(421) A3907, a systemic ASBT inhibitor, improves cholestasis in mice by multiorgan activity and shows translational relevance to humansHepatologyApril 1, 2023Francisco J. Caballero-Camino, Pedro M. Rodrigues, Fredrik Wångsell, Aloña Agirre-Lizaso, Paula Olaizola, Laura Izquierdo-Sanchez, Maria J. Perugorria, Luis Bujanda, Bo Angelin, Sara Straniero, et al.quantitative real-time PCR (RT-qPCR) RNA was extracted from mouse liver and ileum tissues using the Maxwell RSC Instrument (Promega) following the manufacturer’s instructions and subjected to reverse transcription followed

(422) High mobility group box 1, ATP, lipid mediators, and tissue factor are elevated in COVID‐19 patients: HMGB1 as a biomarker of worst prognosisClinical and Translational ScienceApril 1, 2023Amanda Roberta Revoredo Vicentino, Vanderlei da Silva da Silva Fraga-Junior, Matheus Palazzo, Natalia Recardo Amorim Tasmo, Danielle A. S. Rodrigues, Shana Priscila Coutinho Barroso, Sâmila Natiane Ferreira, Anna Cristina Neves Neves‐Borges, Diego Allonso, Marcelo Rosado Fantappié, et al.extractions from nasopharyngeal swab samples were performed using the automated Maxwell System platform (No. AS4500; Promega, Madison, WI). Twenty‐five microliters of total RNA was submitted to cDNA synthesis with high‐capacity cDNA reverse transcription kits

(423) Reducing bias in microbiome research: Comparing methods from sample collection to sequencingFrontiers in MicrobiologyMarch 30, 2023Jolanda Kool, Liza Tymchenko, Sudarshan A. Shetty, Susana Fuentesmin. Samples were centrifuged and the lysates were collected. All lysates were further purified in the Maxwell RSC instrument (Promega, Madison, USA), using the Maxwell RSC blood DNA kit and the Maxwell RSC Fecal kit on a subset

(424) CD8+ cells and small viral reservoirs facilitate post-ART control of SIV in Mauritian cynomolgus macaquesbioRxivMarch 9, 2023Olivia E. Harwood, Lea M. Matschke, Ryan V. Moriarty, Alexis J. Balgeman, Abigail J. Weaver, Amy L. Ellis-Connell, Andrea M. Weiler, Lee C. Winchester, Courtney V. Fletcher, Thomas C. Friedrich, et al.lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene, β-actin, by RT-qPCR.

(425) Experimental ectoparasite removal has a sex‐specific effect on nestling telomere lengthEcology and EvolutionMarch 7, 2023Sarah E. Wolf, Samuel Zhang, Ethan D. ClotfelterCawthon, 2009; Criscuolo et al., 2009), which used DNA extracted from ≤25 μL whole blood via an automated Maxwell® RSC Instrument (Promega, Madison, WI) and Whole Blood DNA Kit (Promega no. AS1520). Relative telomere length was measured

(426) A novel missense variant in the L2HGDH gene in a cat with L‐2‐hydroxyglutaric aciduria and multicystic cerebral lesionsJournal of Veterinary Internal MedicineMarch 7, 2023Matthias Christen, Veronica Gonzalo‐Nadal, Adriana Kaczmarska, Magdalena Dyrka, Julien Guevar, Vidhya Jagannathan, Tosso Leeb, Rodrigo Gutierrez‐QuintanaS1 and S2). Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit, using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). An Illumina TruSeq PCR‐free DNA library with approximately 400 bp

(427) The dogs of Chernobyl: Demographic insights into populations inhabiting the nuclear exclusion zoneScience AdvancesMarch 3, 2023Gabriella J. Spatola, Reuben M. Buckley, Megan Dillon, Emily V. Dutrow, Jennifer A. Betz, Małgorzata Pilot, Heidi G. Parker, Wiesław Bogdanowicz, Rachel Thomas, Ihor Chyzhevskyi, et al.isolated according to the manufacturer’s protocols using the Maxwell RSC Whole Blood DNA Kit and a Maxwell RSC instrument (Promega Corp., Madison, WI). SNP genotyping A total of 406 samples were genotyped using Illumina

(428) Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agentsCellMarch 2, 2023Jidong Zhang, Christine Lair, Christine Roubert, Kwame Amaning, María Belén Barrio, Yannick Benedetti, Zhicheng Cui, Zhongliang Xing, Xiaojun Li, Scott G. Franzblau, et al.ArrayScan VTI,Thermo Fisher,Cellomics ArrayScan VTI RNA extraction: MaxWell RSC Instrument,Promega,Cat#AS4500 Grinder:Fast prep instrument,MP Biomedicals,FastPrep-24™ Classic Instrument 7500 Real-Time PCR system,Applied Biosystems

(429) IPSC reprogramming of two patients with spondyloepimetaphyseal dysplasia (SEMD, biglycan type)Stem Cell ResearchMarch 1, 2023Pauline De Kinderen, Silke Peeters, Laura Rabaut, Geert Mortier, Peter Ponsaerts, Bart Loeys, Aline Verstraeten, Josephina A.N. Meesteranalysis) Genomic DNA was isolated from the patients’ respective fibroblast and iPSC pellets (passage 10) using the Maxwell® RSC Instrument and Maxwell® RSC Cultured Cells DNA Kit (Promega) according to manufacturer’s protocol. Subsequently,

(430) Spanish wastewater reveals the current spread of Monkeypox virusWater ResearchMarch 1, 2023Inés Girón-Guzmán, Azahara Díaz-Reolid, Pilar Truchado, Albert Carcereny, David García-Pedemonte, Bruno Hernáez, Albert Bosch, Rosa María Pintó, Susana Guix, Ana Allende, et al.effects. Nucleic acids extraction (100 µl) of the concentrated samples (300 µl) was performed by the Maxwell® RSC Instrument (Promega) using the Maxwell RSC Pure Food GMO and authentication kit (Promega) and the “Maxwell RSC

(431) Evaluation of two different concentration methods for surveillance of human viruses in sewage and their effects on SARS-CoV-2 sequencingScience of The Total EnvironmentMarch 1, 2023Inés Girón-Guzmán, Azahara Díaz-Reolid, Enric Cuevas-Ferrando, Irene Falcó, Pablo Cano-Jiménez, Iñaki Comas, Alba Pérez-Cataluña, Gloria Sánchezconcentrates, obtained by the AP and the concentration procedure of the TNA kit, was carried out using the Maxwell® RSC Instrument (Promega) with the Maxwell RSC Pure Food GMO and authentication kit (Promega) and the “Maxwell RSC Viral

(432) Identification of neoantigens in oesophageal adenocarcinomaImmunologyMarch 1, 2023Nicholas B, Bailey A, McCann KJ, Wood O, Walker RC, Parker R, Ternette N, Elliott T, Underwood TJ, Johnson P, et al.nitrogen. Ten to twenty 10 μm cryosections were used for nucleic acid extraction using the automated Maxwell® RSC instrument (Promega) with the appropriate sample kit and according to the manufacturer's instructions: Maxwell

(433) Hereditary predisposition to malignant myeloid hemopathies: Caution in use of saliva and guideline based on our experienceFrontiers in OncologyFebruary 27, 2023Alexandre Perani, Sylvie Bourthoumieu, David Rizzo, Jasmine Chauzeix, Benjamin Dauriat, Pascal Turlure, Stéphane Girault, Léa Veyrune, Maxime Roubinet, Jean Feuillard, et al.local ethic committee (registration number: 584-2022-240). 2.2 Molecular studies We isolated DNA with Maxwell RSC instrument (Promega, Madison, WI, USA) using the appropriate kit for each sample type according to the supplier’s

(434) Post-weaning diarrhea in pigs from a single Danish production herd was not associated with the pre-weaning fecal microbiota composition and diversityFrontiers in MicrobiologyFebruary 24, 2023Martin Peter Rydal, Michela Gambino, Josue L. Castro-Mejia, Louise Ladefoged Poulsen, Claus Bøttcher Jørgensen, Jens Peter Nielsencharacterization and to investigate if one or more strains were present. Extraction of DNA was performed on Maxwell® RSC instrument with Maxwell RSC culture cell’s DNA kit (Promega, Madison, USA) and WGS was performed at coverage of

(435) Directed self-assembly of a xenogeneic vascularized endocrine pancreas for type 1 diabetesNature communicationsFebruary 16, 2023Citro A, Neroni A, Pignatelli C, Campo F, Policardi M, Monieri M, Pellegrini S, Dugnani E, Manenti F, Maffia MC, et al.into 200 μl aliquots (n = 2) for RNA extraction and 300 μl aliquots (n = 2) for DNA extraction. The Maxwell® RSC Instrument (Promega) was used to extract the RNAs with the Maxwell® RSC simplyRNA Cells Kit and the DNA with the

(436) Personalized recurrence risk assessment following the birth of a child with a pathogenic de novo mutationNature CommunicationsFebruary 15, 2023Marie Bernkopf, Ummi B. Abdullah, Stephen J. Bush, Katherine A. Wood, Sahar Ghaffari, Eleni Giannoulatou, Nils Koelling, Geoffrey J. Maher, Loïc M. Thibaut, Jonathan Williams, et al.saliva lysates, 2 whole blood samples, 2 urine cell pellets, 6 buccal swabs, 1 semen lysate) on the Maxwell RSC Instrument using the Maxwell RSC Blood DNA kit (both Promega, WI, USA) and following manufacturer’s protocols.

(437) An Italian Multicenter Perspective Harmonization Trial for the Assessment of MET Exon 14 Skipping Mutations in Standard Reference SamplesDiagnostics (Basel)February 8, 2023Paolo Bironzo, Francesco Pepe, Gianluca Russo, Pasquale Pisapia, Gianluca Gragnano, Gabriella Aquino, Silvia Bessi, Simonetta Buglioni, Federico Bartoccini, Giuseppina Ferrero, et al.New Taipei City) and the Maxwell® RSC RNA FFPE Kit (Promega Corporation, Madison, WI, USA) with the Maxwell® RSC instrument (Promega Corporation; Madison, WI, USA) were equally used (n = 2/4, 50% and n = 2/4, 50%). Among those

(438) Impact of polymorphisms in CYP and UGT enzymes and ABC and SLCO1B1 transporters on the pharmacokinetics and safety of desvenlafaxineFrontiers in PharmacologyFebruary 2, 2023Sofía Calleja, Pablo Zubiaur, Dolores Ochoa, Gonzalo Villapalos-García, Gina Mejia-Abril, Paula Soria-Chacartegui, Marcos Navares-Gómez, Alejandro de Miguel, Manuel Román, Samuel Martín-Vílchez, et al.separation, the cell concentrate was resuspended in NaCl 0.9% for the preservation of the blood. A Maxwell® RSC Instrument (Promega, United States) was used for the extraction of DNA. For the genotyping, a QuantStudio 12 k

(439) Vemurafenib and Dabrafenib Downregulates RIPK4 LevelCancers (Basel)February 1, 2023Ewelina Madej, Anna A. Brożyna, Agnieszka Adamczyk, Norbert Wronski, Agnieszka Harazin-Lechowska, Anna Muzyk, Krzysztof Makuch, Michal Markiewicz, Janusz Rys, Agnieszka Wolnicka-Glubisztesting was established. DNA was extracted from tissue sections using a semiautomatic method using Maxwell® RSC Instrument (Promega, Walldorf, Germany). The Maxwell® RSC DNA FFPE kit (Cat. No. AS1450, Promega) was used to isolate

(440) Ultrafast Gene Fusion Assessment for Nonsquamous NSCLCJTO Clinical and Research ReportsFebruary 1, 2023Véronique Hofman, Simon Heeke, Christophe Bontoux, Lara Chalabreysse, Marc Barritault, Pierre Paul Bringuier, Tanguy Fenouil, Nazim Benzerdjeb, Hugues Begueret, Jean Philippe Merlio, et al.processed for NGS analyses using a Genexus system (Thermo Fisher Scientific) after nucleic acid extraction (Maxwell RSC Instrument, Promega Corporation, Madison, WI). Quantification and quality assessment of the extracted nucleic acid

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