Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(421) Identification and Characterisation of Pseudomonas savastanoi pv. savastanoi as the Causal Agent of Olive Knot Disease in Croatian, Slovenian and Portuguese Olive (Olea europaea L.) OrchardsPlants (Basel)January 9, 2023Laura Košćak, Janja Lamovšek, Edyta Đermić, Stefania Tegli, Igor Gruntar, Sara Godenagrowing bacteria on KB and PVF-1 media incubated at 26 °C for 48 h. Bacterial DNA was isolated using a Maxwell® RSC Instrument (Promega, Madison, WI, USA) and Maxwell® RSC Cultured Cells DNA Kit (Promega, Madison, WI, USA), following

(422) Evaluation of automated techniques for extraction of circulating cell-free DNA for implementation in standardized high-throughput workflowsScientific ReportsJanuary 7, 2023Sarah Lehle, Julius Emons, Carolin C. Hack, Felix Heindl, Alexander Hein, Caroline Preuß, Katharina Seitz, Anna L. Zahn, Matthias W. Beckmann, Peter A. Fasching, et al.Hilden, Germany) with the EZ1&EZ2 ccfDNA field test kit (EZ2 kit; QIAGEN, Hilden, Germany) or 2) the Maxwell RSC instrument (Maxwell instrument, Promega, Walldorf, Germany) with the Maxwell kit; we used each set of equipment

(423) ESR1 gene amplification and MAP3K mutations are selected during adjuvant endocrine therapies in relapsing Hormone Receptor-positive, HER2-negative breast cancer (HR+ HER2- BC)PLOS GeneticsJanuary 3, 2023Lorenzo Ferrando, Andrea Vingiani, Anna Garuti, Claudio Vernieri, Antonino Belfiore, Luca Agnelli, Gianpaolo Dagrada, Diana Ivanoiu, Giuseppina Bonizzi, Elisabetta Munzone, et al.cellularity of each sample was recorded. DNA was extracted using the Maxwell RSC DNA FFPE Kit with a Maxwell RSC Instrument (Promega Corporation, Madison, USA). The concentration and purity of DNA samples was measured using

(424) Tumor Microenvironment in Male Breast Carcinoma with Emphasis on Tumor Infiltrating Lymphocytes and PD-L1 ExpressionInternational Journal of Molecular SciencesJanuary 3, 2023Iva Brcic, Andrea Maria Kluba, Theresa Marie Godschachner, Christoph Suppan, Peter Regitnig, Nadia Dandachi, Sigurd Friedwald Lax, Marija Balićperformed with a scalpel to enrich the tumor content. Semi-automated RNA extraction was performed on the Maxwell RSC Instrument according to the manufacturer’s instructions, using the Maxwell RSC FFPE Plus RNA Purification Kit (AS1440,

(425) Tacedinaline (CI-994), a class I HDAC inhibitor, targets intrinsic tumor growth and leptomeningeal dissemination in MYC-driven medulloblastoma while making them susceptible to anti-CD47-induced macrophage phagocytosis via NF-kB-TGM2 driven tumor inflammationJournal for immunotherapy of cancerJanuary 1, 2023Marquardt V, Theruvath J, Pauck D, Picard D, Qin N, Blümel L, Maue M, Bartl J, Ahmadov U, Langini M, et al.from treated or untreated cells was conducted using Trizol (#15 596–018, Thermo Scientific) or the Maxwell RSC Instrument (RSC simplyRNA Tissue, #AS1340, Promega), and complementary DNA (cDNA) was synthesized from 0.5 µg RNA

(426) Microbiota and environmental health monitoring of mouse colonies by metagenomic shotgun sequencingWorld Journal of Microbiology and BiotechnologyJanuary 1, 2023Laura Lupini, Cristian Bassi, Paola Guerriero, Marcello Raspa, Ferdinando Scavizzi, Silvia SabbioniPromega) was added and microbial nucleic acids were isolated using the Promega Maxwell® RSC system (AS4500, Promega), following the manufacturer’s instructions. As for the fecal pellets, for non SPF mice: 5

(427) Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNAHuman GenomicsDecember 31, 2022Jill Deleu, Kathleen Schoofs, Anneleen Decock, Kimberly Verniers, Sofie Roelandt, Angie Denolf, Joke Verreth, Bram De Wilde, Tom Van Maerken, Katleen De Preter, et al.ccfDNA LV Plasma Kit (Promega, AS1480) in combination with the Maxwell RSC Instrument (MAX; Promega, AS4500), and (6) miRNeasy Serum/Plasma Kit (MIR; Qiagen, 217184) (Fig. 1). The MIRA, CCF, MAP and CAT kits

(428) Bacterial Profiles of Brain in Downer Cattle with Unknown EtiologyMicroorganismsDecember 30, 2022Yeong-Jun Park, Gi-Ung Kang, Minsoo Jeong, Vineet Singh, Jongho Kim, Kyunghyun Lee, Eun-Jin Choi, Heui-Jin Kim, Seungjun Lee, Sook-Young Lee, et al.manufacturer’s instruction. DNA and RNA samples from brain tissue were extracted for virus detection through the Maxwell RSC instrument, RSC Blood DNA kit (Promega, Madison, WI, USA), and RSC Viral TNA (Promega) according to the manufacturer’s

(429) A New Indicator to Differentiate Thyroid Follicular Inclusions in Cervical Lymph Nodes from Patients with Thyroid CancerInternational Journal of Molecular SciencesDecember 28, 2022Chieko Otsubo, Zhanna Mussazhanova, Hirokazu Kurohama, Gulzira Shalgimbayeva, Nozomi Ueki, Yuki Matsuoka, Madina Madiyeva, Shinya Sato, Hiroyuki Yamashita, Masahiro Nakashima56 °C. DNA extraction was performed using a Maxwell RSC DNA FFPE Kit (Promega, Madison, WI, USA) and Maxwell RSC Instrument (Promega) according to the manufacturer’s protocol. The concentration of double-strand DNA was quantified

(430) Large–Scale Transposon Mutagenesis Reveals Type III Secretion Effector HopR1 Is a Major Virulence Factor in Pseudomonas syringae pv. actinidiaePlants (Basel)December 27, 2022Takako Ishiga, Nanami Sakata, Giyu Usuki, Viet Tru Nguyen, Kenji Gomi, Yasuhiro Ishigaafter inoculation, total RNA was extracted from the inoculated kiwifruit seedlings and purified using Maxwell® RSC Instrument (Promega) according to the manufacture’s protocol. Total RNA (2 µg) was treated with gDNA Remover (TOYOBO)

(431) Increased HRD score in cisplatin resistant penile cancer cellsBMC CancerDecember 23, 2022Ria Winkelmann, Katrin Bankov, Claudia Döring, Jaroslav Cinatl, Sebastian Grothe, Florian Rothweiler, Martin Michaelis, Christina Schmitt, Peter J. Wild, Melanie Demes, et al.taken as whole slides. The purification of DNA and RNA from FFPE tissue samples was performed using the Maxwell® RSC Instrument (Promega Corporation, Madison, Wisconsin, USA) with the Maxwell® RSC FFPE Plus RNA Kit (Promega Corporation,

(432) Locally Performed HRD Testing for Ovarian Cancer? Yes, We Can!Cancers (Basel)December 21, 2022Gilda Magliacane, Emanuela Brunetto, Silvia Calzavara, Alice Bergamini, Giovanni Battista Pipitone, Giovanna Marra, Miriam Redegalli, Greta Grassini, Emanuela Rabaiotti, Gianluca Taccagni, et al.percentage of tumor cells above 50%. DNA extraction was performed using thee Maxwell® RSC DNA FFPE kit and Maxwell RSC Instrument (Promega, Milan, Italy) and quantified using the Qubit DNA HS Assay Kit on Qubit 3.0 Fluorometer (ThermoFisher Scientific

(433) Clonal transcriptomics identifies mechanisms of chemoresistance and empowers rational design of combination therapieseLifeDecember 16, 2022Sophia A Wild, Ian G Cannell, Ashley Nicholls, Katarzyna Kania, Dario Bressan, Gregory J CRUK IMAXT Grand Challenge Team, Gregory J Sawicka, Kirsty CRUK IMAXT Grand Challenge Team96-well plates were pooled and gDNA extraction of each pooled row of cells was carried out using the Maxwell RSC instrument (Promega) and the ‘Cultured cells DNA extraction kit’ (Promega). These samples were then subjected to

(434) Non-uniform dystrophin re-expression after CRISPR-mediated exon excision in the dystrophin/utrophin double-knockout mouse model of DMDMolecular Therapy - Nucleic AcidsDecember 13, 2022Britt Hanson, Sofia Stenler, Nina Ahlskog, Katarzyna Chwalenia, Nenad Svrzikapa, Anna M.L. Coenen-Stass, Marc S. Weinberg, Matthew J.A. Wood, Thomas C. RobertsTotal RNA was isolated from muscle tissues (∼100 μm sections, or one-sixth of the diaphragm) using a Maxwell RSC Instrument and Maxwell RSC simplyRNA Tissue Kit (both Promega, Southampton, UK), and cDNA was reverse-transcribed

(435) RNA-Seq comparative study reveals molecular effectors linked to the resistance of Pinna nobilis to Haplosporidium pinnae parasiteScientific ReportsDecember 8, 2022Pauline Salis, Claire Peyran, Titouan Morage, Simon de Bernard, Julien Nourikyan, Stéphane Coupé, Robert Bunet, Serge Planes15,260,488). The RNA was automatically extracted using Maxwell® RSC Instrument (Promega, reference: AS4500) and Maxwell® RSC simplyRNA Tissue Kit (Promega-reference: AS1340). RNA‐Seq libraries were generated

(436) Loss of primary cilia promotes inflammation and carcinogenesisEMBO reportsDecember 6, 2022Conception Paul, Ruizhi Tang, Ciro Longobardi, Rossano Lattanzio, Thibaut Eguether, Hulya Turali, Julie Bremond, Chloé Maurizy, Monica Gabola, Sophie Poupeau, et al.inflammatory conditioned medium, as described above. Total RNA was extracted from each sample using the Maxwell® RSC Instrument (Promega, Madison, WI, USA) and Maxwell RSC simplyRNA Blood Kit (AS1380, Promega). High‐quality total

(437) Rapid detection of single nucleotide polymorphisms using the MinION nanopore sequencer: a feasibility study for perioperative precision medicineJA Clinical ReportsDecember 1, 2022Yoshiteru Tabata, Yoshiyuki Matsuo, Yosuke Fujii, Atsufumi Ohta, Kiichi Hirota(AS1400, Promega, Madison, WI, USA) on the Maxwell RSC automated nucleic acid purification platform (AS4500, Promega). Briefly, the sample (0.3 mL) was mixed with lysis buffer (0.3 mL) and proteinase K solution

(438) Utility of ultra-rapid real-time PCR for detection and prevalence of Rickettsia spp. in ticksBMC Veterinary ResearchDecember 1, 2022A-Tai Truong, Bo-Ram Yun, Mi-Sun Yoo, Jiyeon Lim, Subin Min, Soon-Seek Yoon, Young-Min Yun, Jong-Taek Kim, Yun Sang Choacid was extracted using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit on the automated Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Finally, 50 μl of total nucleic

(439) Detecting structural variations with precise breakpoints using low-depth WGS data from a single oxford nanopore MinION flowcellScientific ReportsDecember 1, 2022Henry C. M. Leung, Huijing Yu, Yifan Zhang, Wing Sze Leung, Ivan F. M. Lo, Ho Ming Luk, Wai-Chun Law, Ka Kui Ma, Chak Lim Wong, Yat Sing Wong, et al.the real patient data, DNA was extracted from EDTA blood using Maxwell RSC Whole Blood DNA Kit with Maxwell RSC Instrument (Promega, Spain) and stored at − 80 °C upon sequencing. All DNA samples were prepared using an in-house

(440) Physical separation of haplotypes in dikaryons allows benchmarking of phasing accuracy in Nanopore and HiFi assemblies with Hi-C dataGenome BiologyDecember 1, 2022Hongyu Duan, Ashley W. Jones, Tim Hewitt, Amy Mackenzie, Yiheng Hu, Anna Sharp, David Lewis, Rohit Mago, Narayana M. Upadhyaya, John P. Rathjen, et al.and 9 days post inoculation (dpi) was extracted using the Promega Maxwell® RSC Plant RNA Kit with a Maxwell® RSC instrument (Promega.com.au). The spores were induced for germination by placing on the surface of milli-Q water,

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