Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(441) Phylogenetic analyses of Salmonella detected along the broiler production chain in Trinidad and TobagoPoultry ScienceFebruary 1, 2023Anisa S. Khan, Rian E. Pierneef, Narjol Gonzalez-Escalona, Meghan Maguire, Karla Georges, Woubit Abebe, Abiodun A. Adesiyunpathogen. DNA Extraction and Sequencing DNA was extracted using both Maxwell RSC cultured cells DNA kit with a Maxwell RSC instrument (Promega, Madison, WI) and DNeasy Blood & Tissue Kit (Qiagen, Germany) following the manufacturer's

(442) TMB and BRAF mutation status are independent predictive factors in high-risk melanoma patients with adjuvant anti-PD-1 therapyJournal of Cancer Research and Clinical OncologyFebruary 1, 2023Julia Eckardt, Christopher Schroeder, Peter Martus, Sorin Armeanu-Ebinger, Olga Kelemen, Axel Gschwind, Irina Bonzheim, Thomas Eigentler, Teresa Amaral, Stephan Ossowski, et al.was extracted from macrodissected 5 µm paraffin sections using the Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. 200 ng of genomic DNA (gDNA)

(443) Pain Interference in End Stage Kidney Disease is Associated with Changes in Gut Microbiome Features Before and After Kidney TransplantationPain Management NursingFebruary 1, 2023Mark B. Lockwood, George E. Chlipala, Mark Maeinschein-Cline, Holli A. DeVon, Alicia B. Lichvar, Manpreet K. Samra, Chang G. Park, Maya Campara, Ardith Z. Doorenbos, Lisa M. Tussing-Humphreys, et al.performed. DNA Extraction, Preparation, and Sequencing Genomic DNA was extracted from fecal specimens using a Maxwell RSC instrument (Promega Corporation, Madison, WI, United States) for automated nucleic acid recovery. Libraries were

(444) Transcriptional Insights of Oxidative Stress and Extracellular Traps in Lung Tissues of Fatal COVID-19 CasesInternational journal of molecular sciencesJanuary 31, 2023Hosseini A, Stojkov D, Fettrelet T, Bilyy R, Yousefi S, Simon HU.seven patients and three non-covid-infected individuals using the Maxwell® RSC RNA FFPE Kit in the Maxwell® RSC Instrument (Promega, Dübendorf, Switzerland). The quantity and quality of the extracted RNA were assessed using

(445) DSP-0509, a systemically available TLR7 agonist, exhibits combination effect with immune checkpoint blockade by activating anti-tumor immune effectsFrontiers in ImmunologyJanuary 30, 2023Yosuke Ota, Yasuhiro Nagai, Yuko Hirose, Seiji Hori, Erina Koga-Yamakawa, Ken Eguchi, Kentaro Sumida, Masashi Murata, Hiroki Umehara, Setsuko YamamotoAfter homogenization of the tumors, a Maxwell® RSC simplyRNA Tissue Kit (Promega) was used with the Maxwell® RSC Instrument (Promega) to isolate total RNA. The resulting total RNA was subjected to reverse-transcription-PCR using

(446) The Genetic Background of Hearing Loss in Patients with EVA and Cochlear MalformationGenes (Basel)January 28, 2023Natalia Bałdyga, Dominika Oziębło, Nina Gan, Mariusz Furmanek, Marcin L. Leja, Henryk Skarżyński, Monika Ołdakfamily members, buccal swabs were obtained, and genomic DNA was isolated with the automatic method on a Maxwell RSC instrument (Promega, Walldorf, Germany) according to the manufacturer’s protocol. For 21 probands, custom multigene

(447) Turmeronols (A and B) from Curcuma longa have anti-inflammatory effects in lipopolysaccharide-stimulated BV-2 microglial cells by reducing NF-κB signalingBioscience of Microbiota, Food and HealthJanuary 27, 2023Ryosuke SAJI, Ryusei UCHIO, Arisa FUWA, Chinatsu OKUDA-HANAFUSA, Kengo KAWASAKI, Koutarou MUROYAMA, Shinji MUROSAKI, Yoshihiro YAMAMOTO, Yoshitaka HIROSEcultured cells with a Maxwell® RSC simplyRNA Cells Kit and automated RNA extraction system by using a Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions [27,28,29]. The RNA was eluted

(448) Peripheral blood cellular profile at pre-lymphodepletion is associated with CD19-targeted CAR-T cell-associated neurotoxicityFrontiers in ImmunologyJanuary 16, 2023Serena De Matteis, Michele Dicataldo, Beatrice Casadei, Gianluca Storci, Noemi Laprovitera, Mario Arpinati, Enrico Maffini, Pietro Cortelli, Maria Guarino, Francesca Vaglio, et al.was purified from 200 µl of plasma collected at pre-LD using Maxwell RSC miRNA Plasma/Serum Kit and Maxwell RSC Instrument (Promega, USA). miRNA polyadenylation and reverse transcription were performed using miRCURY LNA RT

(449) Exploration of Patient-Derived Pancreatic Ductal Adenocarcinoma Ex Vivo Tissue for Treatment ResponseAntioxidants (Basel)January 11, 2023Tímea Szekerczés, Arun Kumar Selvam, Carlos Fernández Moro, Soledad Pouso Elduayen, Joakim Dillner, Mikael Björnstedt, Mehran Ghaderiisolated using Maxwell RSC simplyRNA Tissue Kit (AS1340, Promega Corporation, Madison, WI, USA) by Maxwell RSC Instrument (Promega Corporation, Madison, WI, USA), in accordance with the instructions of the manufacturer. cDNAs

(450) SCN9A variant in a family of mixed breed dogs with congenital insensitivity to painJournal of Veterinary Internal MedicineJanuary 11, 2023Rodrigo Gutierrez‐Quintana, Matthias Christen, Kiterie M. E. Faller, Julien Guevar, Vidhya Jagannathan, Tosso Leebbuccal swabs with the Maxwell RSC Whole Blood Kit and the RSC Buccal Swab DNA Kit, respectively, using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.3.2 Whole‐genome sequencing An Illumina TruSeq PCR‐free DNA library

(451) Identification and Characterisation of Pseudomonas savastanoi pv. savastanoi as the Causal Agent of Olive Knot Disease in Croatian, Slovenian and Portuguese Olive (Olea europaea L.) OrchardsPlants (Basel)January 9, 2023Laura Košćak, Janja Lamovšek, Edyta Đermić, Stefania Tegli, Igor Gruntar, Sara Godenagrowing bacteria on KB and PVF-1 media incubated at 26 °C for 48 h. Bacterial DNA was isolated using a Maxwell® RSC Instrument (Promega, Madison, WI, USA) and Maxwell® RSC Cultured Cells DNA Kit (Promega, Madison, WI, USA), following

(452) Evaluation of automated techniques for extraction of circulating cell-free DNA for implementation in standardized high-throughput workflowsScientific ReportsJanuary 7, 2023Sarah Lehle, Julius Emons, Carolin C. Hack, Felix Heindl, Alexander Hein, Caroline Preuß, Katharina Seitz, Anna L. Zahn, Matthias W. Beckmann, Peter A. Fasching, et al.Hilden, Germany) with the EZ1&EZ2 ccfDNA field test kit (EZ2 kit; QIAGEN, Hilden, Germany) or 2) the Maxwell RSC instrument (Maxwell instrument, Promega, Walldorf, Germany) with the Maxwell kit; we used each set of equipment

(453) Tumor Microenvironment in Male Breast Carcinoma with Emphasis on Tumor Infiltrating Lymphocytes and PD-L1 ExpressionInternational Journal of Molecular SciencesJanuary 3, 2023Iva Brcic, Andrea Maria Kluba, Theresa Marie Godschachner, Christoph Suppan, Peter Regitnig, Nadia Dandachi, Sigurd Friedwald Lax, Marija Balićperformed with a scalpel to enrich the tumor content. Semi-automated RNA extraction was performed on the Maxwell RSC Instrument according to the manufacturer’s instructions, using the Maxwell RSC FFPE Plus RNA Purification Kit (AS1440,

(454) ESR1 gene amplification and MAP3K mutations are selected during adjuvant endocrine therapies in relapsing Hormone Receptor-positive, HER2-negative breast cancer (HR+ HER2- BC)PLOS GeneticsJanuary 3, 2023Lorenzo Ferrando, Andrea Vingiani, Anna Garuti, Claudio Vernieri, Antonino Belfiore, Luca Agnelli, Gianpaolo Dagrada, Diana Ivanoiu, Giuseppina Bonizzi, Elisabetta Munzone, et al.cellularity of each sample was recorded. DNA was extracted using the Maxwell RSC DNA FFPE Kit with a Maxwell RSC Instrument (Promega Corporation, Madison, USA). The concentration and purity of DNA samples was measured using

(455) Microbiota and environmental health monitoring of mouse colonies by metagenomic shotgun sequencingWorld Journal of Microbiology and BiotechnologyJanuary 1, 2023Laura Lupini, Cristian Bassi, Paola Guerriero, Marcello Raspa, Ferdinando Scavizzi, Silvia SabbioniPromega) was added and microbial nucleic acids were isolated using the Promega Maxwell® RSC system (AS4500, Promega), following the manufacturer’s instructions. As for the fecal pellets, for non SPF mice: 5

(456) Tacedinaline (CI-994), a class I HDAC inhibitor, targets intrinsic tumor growth and leptomeningeal dissemination in MYC-driven medulloblastoma while making them susceptible to anti-CD47-induced macrophage phagocytosis via NF-kB-TGM2 driven tumor inflammationJournal for immunotherapy of cancerJanuary 1, 2023Marquardt V, Theruvath J, Pauck D, Picard D, Qin N, Blümel L, Maue M, Bartl J, Ahmadov U, Langini M, et al.from treated or untreated cells was conducted using Trizol (#15 596–018, Thermo Scientific) or the Maxwell RSC Instrument (RSC simplyRNA Tissue, #AS1340, Promega), and complementary DNA (cDNA) was synthesized from 0.5 µg RNA

(457) Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNAHuman GenomicsDecember 31, 2022Jill Deleu, Kathleen Schoofs, Anneleen Decock, Kimberly Verniers, Sofie Roelandt, Angie Denolf, Joke Verreth, Bram De Wilde, Tom Van Maerken, Katleen De Preter, et al.ccfDNA LV Plasma Kit (Promega, AS1480) in combination with the Maxwell RSC Instrument (MAX; Promega, AS4500), and (6) miRNeasy Serum/Plasma Kit (MIR; Qiagen, 217184) (Fig. 1). The MIRA, CCF, MAP and CAT kits

(458) Bacterial Profiles of Brain in Downer Cattle with Unknown EtiologyMicroorganismsDecember 30, 2022Yeong-Jun Park, Gi-Ung Kang, Minsoo Jeong, Vineet Singh, Jongho Kim, Kyunghyun Lee, Eun-Jin Choi, Heui-Jin Kim, Seungjun Lee, Sook-Young Lee, et al.manufacturer’s instruction. DNA and RNA samples from brain tissue were extracted for virus detection through the Maxwell RSC instrument, RSC Blood DNA kit (Promega, Madison, WI, USA), and RSC Viral TNA (Promega) according to the manufacturer’s

(459) A New Indicator to Differentiate Thyroid Follicular Inclusions in Cervical Lymph Nodes from Patients with Thyroid CancerInternational Journal of Molecular SciencesDecember 28, 2022Chieko Otsubo, Zhanna Mussazhanova, Hirokazu Kurohama, Gulzira Shalgimbayeva, Nozomi Ueki, Yuki Matsuoka, Madina Madiyeva, Shinya Sato, Hiroyuki Yamashita, Masahiro Nakashima56 °C. DNA extraction was performed using a Maxwell RSC DNA FFPE Kit (Promega, Madison, WI, USA) and Maxwell RSC Instrument (Promega) according to the manufacturer’s protocol. The concentration of double-strand DNA was quantified

(460) Large–Scale Transposon Mutagenesis Reveals Type III Secretion Effector HopR1 Is a Major Virulence Factor in Pseudomonas syringae pv. actinidiaePlants (Basel)December 27, 2022Takako Ishiga, Nanami Sakata, Giyu Usuki, Viet Tru Nguyen, Kenji Gomi, Yasuhiro Ishigaafter inoculation, total RNA was extracted from the inoculated kiwifruit seedlings and purified using Maxwell® RSC Instrument (Promega) according to the manufacture’s protocol. Total RNA (2 µg) was treated with gDNA Remover (TOYOBO)

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