Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(401) PKD1 Nonsense Variant in a Lagotto Romagnolo Family with Polycystic Kidney DiseaseGenes (Basel)June 1, 2023Michaela Drögemüller, Nadine Klein, Rikke Lill Steffensen, Miriam Keiner, Vidhya Jagannathan, Tosso LeebBiobank, were used. Genomic DNA was isolated from EDTA blood using the Maxwell RSC Whole Blood Kit on a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.3. Whole Genome Sequencing (WGS) Three Illumina TruSeq PCR-free

(402) Two sporadic cases of childhood-onset Hailey-Hailey disease with superimposed mosaicismEuropean Journal of Human GeneticsJune 1, 2023Yasuhiko Asahina, Umi Tahara, Satomi Aoki, Kazuhiko Nakabayashi, Chiharu Tateishi, Daisuke Hayashi, Masayuki Amagai, Daisuke Tsuruta, Akiharu Kubowas collected. Genomic DNA was obtained from the epidermis and peripheral blood leukocytes using a Maxwell RSC Instrument and Maxwell RSC Blood DNA Kit (Promega, Madison, WI). Targeted exome sequencing Targeted exome sequencing,

(403) Cell-free DNA as a potential biomarker of differentiation and toxicity in cardiac organoidseLifeJune 1, 2023Silver B, Gerrish K, Tokar E.Kit AS1610) and cfDNA from media (Promega, ccfDNA Plasma Kit AS1480) were extracted using the Promega Maxwell RSC instrument. RNA quality and purity were assessed using a NanoDrop 2000 spectrophotometer, and DNA concentrations

(404) Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in HaitiInfectious Diseases of PovertyMay 22, 2023Jeanne Perpétue Vincent, Alexandre Valcena Existe, Kanako Komaki-Yasuda, Jacques Boncy, Shigeyuki Kanoa diameter of 3 mm using a Maxwell® RSC DNA FFPE Kit (Promega, Madison, WI, USA) and the automated Maxwell® RSC Instrument (Promega, Madison, WI, USA). Minor modifications to the manufacturer’s guidelines can be found in a

(405) RNA-seq analysis to identify genes related to resting egg production of panarctic Daphnia pulexBMC GenomicsMay 17, 2023Natsumi Maruoka, Takashi Makino, Jotaro Urabelonger observed. RNA was extracted using a Maxwell RSC Plant RNA Kit with a Maxwell® RSC Instrument AS4500 (Promega, Madison, WI, USA) following the manufacturer’s protocol and RNA concentrations were measured

(406) Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus DetectionBiosensors (Basel)May 12, 2023Francesca Costantini, Nicola Lovecchio, Manasa Nandimandalam, Ariana Manglli, Francesco Faggioli, Mara Biasin, Cesare Manetti, Pio Federico Roversi, Augusto Nascetti, Giampiero de Cesare, et al.performed on VeroE6 cells, and RNA was extracted from six days post-infection (dpi) supernatants using the Maxwell® RSC Instrument with Maxwell® RSC Viral Total Nucleic Acid Purification (Promega, Fitchburg, WI, USA). The RNA extracts

(407) A Real-World Experience from a Single Center (LPCE, Nice, France) Highlights the Urgent Need to Abandon Immunohistochemistry for ROS1 Rearrangement Screening of Advanced Non-Squamous Non-Small Cell Lung CancerJournal of Personalized MedicineMay 10, 2023Véronique Hofman, Samantha Goffinet, Christophe Bontoux, Elodie Long-Mira, Sandra Lassalle, Marius Ilié, Paul Hofmanpositive. 2.2. Next Generation Sequencing The nucleic acid extraction was conducted using either the Maxwell RSC Instrument (Promega, Madison, WI, USA) in combination with the Maxwell RSC FFPE Plus DNA kit or the Maxwell RSC RNA FFPE kit

(408) New, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samplesScientific ReportsMay 5, 2023Aleksandra Gajda-Walczak, Agnieszka Potęga, Agata Kowalczyk, Slawomir Sek, Sebastian Zięba, Artur Kowalik, Andrzej Kudelski, Anna M. Nowickaisolated from 300 μL whole blood with Maxwell® RSC Whole Blood DNA Kit (Promega, Madison, WI, USA) using Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to manufacturer protocol. Two microliters of isolated DNA were

(409) The DNA integrity number and concentration are useful parameters for successful comprehensive genomic profiling test for cancer using formalin‐fixed paraffin embedded tissuePathology InternationalMay 1, 2023Emmy Yanagita, Hiroshi Yamada, Tetsuro Kobayashi, Eriko Aimono, Kohei Nakamura, Akira Hirasawa, Hiroshi Nishihara3 Target amplicon sequence Genomic DNA, which was extracted from thin sliced FFPE tissue using the Maxwell RSC Instrument（Promega), was applied for library construction with the GeneRead DNAseq Targeted Panels V2 (Human Comprehensive

(410) A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiontAnimal MicrobiomeApril 29, 2023Juliana Botero, Atena Sadat Sombolestani, Margo Cnockaert, Charlotte Peeters, Wim Borremans, Luc De Vuyst, Nicolas J. Vereecken, Denis Michez, Guy Smagghe, German Bonilla-Rosso, et al.R-79672, R-79673 and R-79674 was extracted using the Maxwell Tissue DNA kit (Promega, USA) and the Maxwell RSC instrument according to the manufacturer’s instructions; genomic DNA of isolates R-83493, R-83526, R-83534, R-83540

(411) Development and validation of a multivariable genotype-informed gestational diabetes prediction algorithm for clinical use in the Mexican population: insights into susceptibility mechanismsBMJ Open Diabetes Research & CareApril 21, 2023Mirella Zulueta, Héctor Gallardo-Rincón, Luis Alberto Martinez-Juarez, Julieta Lomelin-Gascon, Janinne Ortega-Montiel, Alejandra Montoya, Leire Mendizabal, Maddi Arregi, María de los Angeles Martinez-Martinez, Eneida del Socorro Camarillo Romero, et al.SNP genotyping Genomic DNA was extracted from EDTA-stabilized blood samples taken during the OGTT using the Maxwell RSC instrument (Promega, Dubendorf, Switzerland). Genotyping was performed by iPLEX MassARRAY PCR using the Agena platform

(412) Whole exome sequencing analysis of canine urothelial carcinomas without BRAF V595E mutation: Short in-frame deletions in BRAF and MAP2K1 suggest alternative mechanisms for MAPK pathway disruptionPLOS GeneticsApril 20, 2023Rachael Thomas, Claire A. Wiley, Emma L. Droste, James Robertson, Brant A. Inman, Matthew Breenwere pelleted by centrifugation, rinsed with sterile PBS, and processed for DNA extraction using a Maxwell RSC Instrument and Cell DNA Purification Kit (Promega, Madison WI). DNA samples were screened using ddPCR analysis

(413) Analytical Performance of a Highly Sensitive System to Detect Gene Variants Using Next-Generation Sequencing for Lung Cancer Companion DiagnosticsDiagnostics (Basel)April 19, 2023Kikuya Kato, Jiro Okami, Harumi Nakamura, Keiichiro Honma, Yoshiharu Sato, Seiji Nakamura, Yoji Kukita, Shin-ichi Nakatsuka, Masahiko Higashiyamathe Osaka International Cancer Institute. DNA and RNA were then purified from these samples using a Maxwell RSC instrument (Promega Corporation, Madison, WI, USA). 2.2. Library Preparation and Sequencing of DNA Modules PCR amplification

(414) A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in TilapiaVirusesApril 14, 2023Shayma Alathari, Dominique L. Chaput, Luis M. Bolaños, Andrew Joseph, Victoria L. N. Jackson, David Verner-Jeffreys, Richard Paley, Charles R. Tyler, Ben Temperton[11]. Template DNA was recovered (total nucleic acid kit (ThermoFisher, Heysham, UK), extracted in a Maxwell® RSC Instrument (Promega, Southampton, UK)). Each primer pair was individually tested against the template DNA, following

(415) Gene Fusion Detection in NSCLC Routine Clinical Practice: Targeted-NGS or FISH?CellsApril 11, 2023Lorenza Pecciarini, Emanuela Brunetto, Greta Grassini, Valeria De Pascali, Francesca Rita Ogliari, Anna Talarico, Giovanna Marra, Gilda Magliacane, Miriam Redegalli, Gianluigi Arrigoni, et al.extraction. Total RNA was extracted from all 210 FFPE samples by using the Maxwell RSC RNA FFPE Kit and the Maxwell RSC Instrument (Promega, Milan, Italy), according to the manufacturer’s instructions. Elution was performed in 50 μL

(416) Structural basis of spike RBM-specific human antibodies counteracting broad SARS-CoV-2 variantsCommunications biologyApril 11, 2023Shitaoka K, Higashiura A, Kawano Y, Yamamoto A, Mizoguchi Y, Hashiguchi T, Nishimichi N, Huang S, Ito A, Ohki S, et al.RNA Sequencing Viral RNA was extracted from virus-infected culture medium by using Maxwell RSC Instrument (Promega, AS4500). cDNA preparation and amplification were done in accordance with protocols published by the ARTIC network

(417) Optimizing mycobacteria molecular diagnostics: No decontamination! Human DNA depletion? Greener storage at 4 °C!Frontiers in MicrobiologyApril 11, 2023Prajwal Prajwal, Turlough Neary, Katja Rohrbach, Pascal Bittel, Pauline C. Göller, Thorsten Buch, Sebastian Dümcke, Peter M. KellerThe nucleic acids were extracted according to manufacturer’s instructions (Promega, instructions for AS4500) and eluted with 50 μL of elution buffer provided by the supplier. 2.2. Decontamination and human DNA

(418) DUSP6 is a memory retention feedback regulator of ERK signaling for cellular resilience of human pluripotent stem cells in response to dissociationScientific ReportsApril 7, 2023Dae Hoon Yoo, Young Sam Im, Ji Young Oh, Dayeon Gil, Yong-Ou Kim37 °C. RNA-seq Total RNA was isolated from hPSCs using the Maxwell RSC RNA FFPE kit and automated Maxwell RSC Instrument (Promega) according to the manufacturer’s instructions. RNA concentration was determined using Quant-IT

(419) A Novel Alu Element Insertion in ATM Induces Exon Skipping in Suspected HBOC PatientsHuman MutationApril 4, 2023Janin Klein, Aldrige B. Allister, Gunnar Schmidt, Annette Otto, Kai Heinecke, Jördis Bax-Knoche, Carmela Beger, Sarah Becker, Stephan Bartels, Tim Ripperger, et al.enriched by microdissection. DNA was extracted with a Maxwell RSC DNA FFPE Kit (Promega, USA) on a Maxwell RSC instrument according to the manufacturer's recommendations. Subsequently, DNA samples were quantified with a Qubit 2.0 Fluorometer

(420) A3907, a systemic ASBT inhibitor, improves cholestasis in mice by multi-organ activity and shows translational relevance to humansHepatologyApril 3, 2023Francisco J. Caballero-Camino, Pedro M. Rodrigues, Fredrik Wångsell, Aloña Agirre-Lizaso, Paula Olaizola, Laura Izquierdo-Sanchez, Maria J. Perugorria, Luis Bujanda, Bo Angelin, Sara Straniero, et al.quantitative real-time PCR (RT-qPCR) RNA was extracted from mouse liver and ileum tissues using the Maxwell RSC Instrument (Promega) following the manufacturer’s instructions and subjected to reverse transcription followed

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