Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(381) A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiontAnimal MicrobiomeApril 29, 2023Juliana Botero, Atena Sadat Sombolestani, Margo Cnockaert, Charlotte Peeters, Wim Borremans, Luc De Vuyst, Nicolas J. Vereecken, Denis Michez, Guy Smagghe, German Bonilla-Rosso, et al.R-79672, R-79673 and R-79674 was extracted using the Maxwell Tissue DNA kit (Promega, USA) and the Maxwell RSC instrument according to the manufacturer’s instructions; genomic DNA of isolates R-83493, R-83526, R-83534, R-83540

(382) Development and validation of a multivariable genotype-informed gestational diabetes prediction algorithm for clinical use in the Mexican population: insights into susceptibility mechanismsBMJ Open Diabetes Research & CareApril 21, 2023Mirella Zulueta, Héctor Gallardo-Rincón, Luis Alberto Martinez-Juarez, Julieta Lomelin-Gascon, Janinne Ortega-Montiel, Alejandra Montoya, Leire Mendizabal, Maddi Arregi, María de los Angeles Martinez-Martinez, Eneida del Socorro Camarillo Romero, et al.SNP genotyping Genomic DNA was extracted from EDTA-stabilized blood samples taken during the OGTT using the Maxwell RSC instrument (Promega, Dubendorf, Switzerland). Genotyping was performed by iPLEX MassARRAY PCR using the Agena platform

(383) Whole exome sequencing analysis of canine urothelial carcinomas without BRAF V595E mutation: Short in-frame deletions in BRAF and MAP2K1 suggest alternative mechanisms for MAPK pathway disruptionPLOS GeneticsApril 20, 2023Rachael Thomas, Claire A. Wiley, Emma L. Droste, James Robertson, Brant A. Inman, Matthew Breenwere pelleted by centrifugation, rinsed with sterile PBS, and processed for DNA extraction using a Maxwell RSC Instrument and Cell DNA Purification Kit (Promega, Madison WI). DNA samples were screened using ddPCR analysis

(384) Analytical Performance of a Highly Sensitive System to Detect Gene Variants Using Next-Generation Sequencing for Lung Cancer Companion DiagnosticsDiagnostics (Basel)April 19, 2023Kikuya Kato, Jiro Okami, Harumi Nakamura, Keiichiro Honma, Yoshiharu Sato, Seiji Nakamura, Yoji Kukita, Shin-ichi Nakatsuka, Masahiko Higashiyamathe Osaka International Cancer Institute. DNA and RNA were then purified from these samples using a Maxwell RSC instrument (Promega Corporation, Madison, WI, USA). 2.2. Library Preparation and Sequencing of DNA Modules PCR amplification

(385) A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in TilapiaVirusesApril 14, 2023Shayma Alathari, Dominique L. Chaput, Luis M. Bolaños, Andrew Joseph, Victoria L. N. Jackson, David Verner-Jeffreys, Richard Paley, Charles R. Tyler, Ben Temperton[11]. Template DNA was recovered (total nucleic acid kit (ThermoFisher, Heysham, UK), extracted in a Maxwell® RSC Instrument (Promega, Southampton, UK)). Each primer pair was individually tested against the template DNA, following

(386) Structural basis of spike RBM-specific human antibodies counteracting broad SARS-CoV-2 variantsCommunications biologyApril 11, 2023Shitaoka K, Higashiura A, Kawano Y, Yamamoto A, Mizoguchi Y, Hashiguchi T, Nishimichi N, Huang S, Ito A, Ohki S, et al.RNA Sequencing Viral RNA was extracted from virus-infected culture medium by using Maxwell RSC Instrument (Promega, AS4500). cDNA preparation and amplification were done in accordance with protocols published by the ARTIC network

(387) Optimizing mycobacteria molecular diagnostics: No decontamination! Human DNA depletion? Greener storage at 4 °C!Frontiers in MicrobiologyApril 11, 2023Prajwal Prajwal, Turlough Neary, Katja Rohrbach, Pascal Bittel, Pauline C. Göller, Thorsten Buch, Sebastian Dümcke, Peter M. KellerThe nucleic acids were extracted according to manufacturer’s instructions (Promega, instructions for AS4500) and eluted with 50 μL of elution buffer provided by the supplier. 2.2. Decontamination and human DNA

(388) Gene Fusion Detection in NSCLC Routine Clinical Practice: Targeted-NGS or FISH?CellsApril 11, 2023Lorenza Pecciarini, Emanuela Brunetto, Greta Grassini, Valeria De Pascali, Francesca Rita Ogliari, Anna Talarico, Giovanna Marra, Gilda Magliacane, Miriam Redegalli, Gianluigi Arrigoni, et al.extraction. Total RNA was extracted from all 210 FFPE samples by using the Maxwell RSC RNA FFPE Kit and the Maxwell RSC Instrument (Promega, Milan, Italy), according to the manufacturer’s instructions. Elution was performed in 50 μL

(389) DUSP6 is a memory retention feedback regulator of ERK signaling for cellular resilience of human pluripotent stem cells in response to dissociationScientific ReportsApril 7, 2023Dae Hoon Yoo, Young Sam Im, Ji Young Oh, Dayeon Gil, Yong-Ou Kim37 °C. RNA-seq Total RNA was isolated from hPSCs using the Maxwell RSC RNA FFPE kit and automated Maxwell RSC Instrument (Promega) according to the manufacturer’s instructions. RNA concentration was determined using Quant-IT

(390) A Novel Alu Element Insertion in ATM Induces Exon Skipping in Suspected HBOC PatientsHuman MutationApril 4, 2023Janin Klein, Aldrige B. Allister, Gunnar Schmidt, Annette Otto, Kai Heinecke, Jördis Bax-Knoche, Carmela Beger, Sarah Becker, Stephan Bartels, Tim Ripperger, et al.enriched by microdissection. DNA was extracted with a Maxwell RSC DNA FFPE Kit (Promega, USA) on a Maxwell RSC instrument according to the manufacturer's recommendations. Subsequently, DNA samples were quantified with a Qubit 2.0 Fluorometer

(391) A3907, a systemic ASBT inhibitor, improves cholestasis in mice by multi-organ activity and shows translational relevance to humansHepatologyApril 3, 2023Francisco J. Caballero-Camino, Pedro M. Rodrigues, Fredrik Wångsell, Aloña Agirre-Lizaso, Paula Olaizola, Laura Izquierdo-Sanchez, Maria J. Perugorria, Luis Bujanda, Bo Angelin, Sara Straniero, et al.quantitative real-time PCR (RT-qPCR) RNA was extracted from mouse liver and ileum tissues using the Maxwell RSC Instrument (Promega) following the manufacturer’s instructions and subjected to reverse transcription followed

(392) High mobility group box 1, ATP, lipid mediators, and tissue factor are elevated in COVID‐19 patients: HMGB1 as a biomarker of worst prognosisClinical and Translational ScienceApril 1, 2023Amanda Roberta Revoredo Vicentino, Vanderlei da Silva da Silva Fraga-Junior, Matheus Palazzo, Natalia Recardo Amorim Tasmo, Danielle A. S. Rodrigues, Shana Priscila Coutinho Barroso, Sâmila Natiane Ferreira, Anna Cristina Neves Neves‐Borges, Diego Allonso, Marcelo Rosado Fantappié, et al.extractions from nasopharyngeal swab samples were performed using the automated Maxwell System platform (No. AS4500; Promega, Madison, WI). Twenty‐five microliters of total RNA was submitted to cDNA synthesis with high‐capacity cDNA reverse transcription kits

(393) Reducing bias in microbiome research: Comparing methods from sample collection to sequencingFrontiers in MicrobiologyMarch 30, 2023Jolanda Kool, Liza Tymchenko, Sudarshan A. Shetty, Susana Fuentesmin. Samples were centrifuged and the lysates were collected. All lysates were further purified in the Maxwell RSC instrument (Promega, Madison, USA), using the Maxwell RSC blood DNA kit and the Maxwell RSC Fecal kit on a subset

(394) CD8+ cells and small viral reservoirs facilitate post-ART control of SIV in Mauritian cynomolgus macaquesbioRxivMarch 9, 2023Olivia E. Harwood, Lea M. Matschke, Ryan V. Moriarty, Alexis J. Balgeman, Abigail J. Weaver, Amy L. Ellis-Connell, Andrea M. Weiler, Lee C. Winchester, Courtney V. Fletcher, Thomas C. Friedrich, et al.lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene, β-actin, by RT-qPCR.

(395) Experimental ectoparasite removal has a sex‐specific effect on nestling telomere lengthEcology and EvolutionMarch 7, 2023Sarah E. Wolf, Samuel Zhang, Ethan D. ClotfelterCawthon, 2009; Criscuolo et al., 2009), which used DNA extracted from ≤25 μL whole blood via an automated Maxwell® RSC Instrument (Promega, Madison, WI) and Whole Blood DNA Kit (Promega no. AS1520). Relative telomere length was measured

(396) A novel missense variant in the L2HGDH gene in a cat with L‐2‐hydroxyglutaric aciduria and multicystic cerebral lesionsJournal of Veterinary Internal MedicineMarch 7, 2023Matthias Christen, Veronica Gonzalo‐Nadal, Adriana Kaczmarska, Magdalena Dyrka, Julien Guevar, Vidhya Jagannathan, Tosso Leeb, Rodrigo Gutierrez‐QuintanaS1 and S2). Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit, using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). An Illumina TruSeq PCR‐free DNA library with approximately 400 bp

(397) The dogs of Chernobyl: Demographic insights into populations inhabiting the nuclear exclusion zoneScience AdvancesMarch 3, 2023Gabriella J. Spatola, Reuben M. Buckley, Megan Dillon, Emily V. Dutrow, Jennifer A. Betz, Małgorzata Pilot, Heidi G. Parker, Wiesław Bogdanowicz, Rachel Thomas, Ihor Chyzhevskyi, et al.isolated according to the manufacturer’s protocols using the Maxwell RSC Whole Blood DNA Kit and a Maxwell RSC instrument (Promega Corp., Madison, WI). SNP genotyping A total of 406 samples were genotyped using Illumina

(398) Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agentsCellMarch 2, 2023Jidong Zhang, Christine Lair, Christine Roubert, Kwame Amaning, María Belén Barrio, Yannick Benedetti, Zhicheng Cui, Zhongliang Xing, Xiaojun Li, Scott G. Franzblau, et al.ArrayScan VTI,Thermo Fisher,Cellomics ArrayScan VTI RNA extraction: MaxWell RSC Instrument,Promega,Cat#AS4500 Grinder:Fast prep instrument,MP Biomedicals,FastPrep-24™ Classic Instrument 7500 Real-Time PCR system,Applied Biosystems

(399) Evaluation of two different concentration methods for surveillance of human viruses in sewage and their effects on SARS-CoV-2 sequencingScience of The Total EnvironmentMarch 1, 2023Inés Girón-Guzmán, Azahara Díaz-Reolid, Enric Cuevas-Ferrando, Irene Falcó, Pablo Cano-Jiménez, Iñaki Comas, Alba Pérez-Cataluña, Gloria Sánchezconcentrates, obtained by the AP and the concentration procedure of the TNA kit, was carried out using the Maxwell® RSC Instrument (Promega) with the Maxwell RSC Pure Food GMO and authentication kit (Promega) and the “Maxwell RSC Viral

(400) IPSC reprogramming of two patients with spondyloepimetaphyseal dysplasia (SEMD, biglycan type)Stem Cell ResearchMarch 1, 2023Pauline De Kinderen, Silke Peeters, Laura Rabaut, Geert Mortier, Peter Ponsaerts, Bart Loeys, Aline Verstraeten, Josephina A.N. Meesteranalysis) Genomic DNA was isolated from the patients’ respective fibroblast and iPSC pellets (passage 10) using the Maxwell® RSC Instrument and Maxwell® RSC Cultured Cells DNA Kit (Promega) according to manufacturer’s protocol. Subsequently,

Be the First to Write a Review!