Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(381) A Comparative Analysis of NOX4 Protein Expression in Malignant and Non-Malignant Thyroid TumorsCurrent Issues in Molecular BiologyJuly 13, 2023Salma Fenniche, Mohamed Oukabli, Yassire Oubaddou, Hafsa Chahdi, Amal Damiri, Abir Alghuzlan, Abdelilah Laraqui, Nadia Dakka, Youssef Bakri, Corinne Dupuy, et al.DNA was extracted using the ‘Maxwell® RSC DNA FFPE Kit’ from Promega/‘Maxwell® RSC Instruments (Cat.# AS4500, AS8500)’ according to the manufacturer’s protocols (ASB1450). The extracted genomic DNA was sequenced

(382) A low-coverage 3′ RNA-seq to detect homeolog expression in polyploid wheatNAR Genomics and BioinformaticsJuly 12, 2023Jianqiang Sun, Moeko Okada, Toshiaki Tameshige, Rie Shimizu-Inatsugi, Reiko Akiyama, Atsushi J Nagano, Jun Sese, Kentaro K Shimizuand H2SO4 to adjust pH 5.2). The total RNA was extracted using the Maxwell® RSC Plant RNA Kit with Maxwell® RSC Instrument (Promega Corp., Madison, WI) according to the manufacturer’s instructions. For each of the six CS RNA

(383) Expression and possible functions of a horizontally transferred glycosyl hydrolase gene, GH6-1, in Ciona embryogenesisEvoDevoJuly 11, 2023Kun-Lung Li, Keisuke Nakashima, Kanako Hisata, Noriyuki Satohefficiency. Genomic DNA of 15–30 embryos (12–16 hpf) was extracted with a Maxwell RSC Blood DNA Kit on a Maxwell RSC Instrument (Promega). Extracted genomic DNA was amplified with primers targeting a part of the GH6-1 gene; Forward:

(384) Secondary leiomyosarcoma of the nasal cavity in a treated patient with possible hereditary retinoblastoma with germline reciprocal translocation of RB1 and DMXL1 and somatic TP53 mutation: A case reportMolecular and Clinical OncologyJuly 10, 2023Toshinari Yagi, Harumi Nakamura, Yoji Kukita, Toru Wakamatsu, Hironari Tamiya, Shou Nakai, Makiyo Watanabe, Shigeki Kakunaga, Haruna Takami, Rie Suzuki, et al.formalin-fixed, paraffin-embedded (FFPE) tissues RNAs were extracted from slices of FFPE tissue using Maxwell RSC Instrument and Maxwell RCS RNA FFPE Kit (Promega) and their concentrations were measured with the QuantiFluor RNA System

(385) Evolution of a globally unique SARS-CoV-2 Spike E484T monoclonal antibody escape mutation in a persistently infected, immunocompromised individualVirus EvolutionJuly 7, 2023Peter J Halfmann, Nicholas R Minor, Luis A Haddock III, Robert Maddox, Gage K Moreno, Katarina M Braun, David A Baker, Kasen K Riemersa, Ankur Prasad, Kirsten J Alman, et al.isolated viral RNA from an additional nasopharyngeal swab sample using the Total Nucleic Acid kit for the Maxwell RSC instrument (Promega, Madison, WI). We used the N1 qRT-PCR assay developed by the United States Centers for Disease

(386) Investigations on SARS-CoV-2 and other coronaviruses in mink farms in France at the end of the first year of COVID-19 pandemicbioRxivJuly 7, 2023Marine Wasniewski, Franck Boué, Céline Richomme, Etienne Simon-Lorière, Sylvie Van der Werf, Flora Donati, Vincent Enouf, Yannick Blanchard, Véronique Beven, Estelle Leperchois, et al.Lysis buffer. RNA extraction was performed with the Maxwell RSC PureFood GMO and Authentication kit using a Maxwell RSC instrument (Promega France, Charbonnières les Bains, France), according to the manufacturer’s instructions. RNA

(387) A Two-Step Diagnostic Approach for NTRK Gene Fusion Detection in Biliary Tract and Pancreatic AdenocarcinomasThe OncologistJuly 5, 2023Anne Demols, Laureen Rocq, Luis Perez-Casanova, Manon Charry, Nancy De Nève, Ali Ramadhan, Claude Van Campenhout, Sarah De Clercq, Calliope Maris, Jean Closset, et al.Quantification Nucleic acids were extracted from FFPE tumor samples, after macrodissection of the tumor area, using the Maxwell RSC Instrument (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions and as already

(388) Novel potentially pathogenic variants detected in genes causing intellectual disability and epilepsy in Polish familiesneurogeneticsJuly 5, 2023S. Skoczylas, P. Jakiel, T. Płoszaj, K. Gadzalska, M. Borowiec, A. Pastorczak, H. Moczulska, M. Malarska, A. Eckersdorf-Mastalerz, E. Budzyńska, et al.list of genes is described in Supplementary Table 1. DNA was extracted from peripheral blood using Maxwell® RSC Instrument (Maxwell® RSC Blood DNA Kit, Promega, Madison, USA). The library was prepared using the Agilent SureSelectQXT

(389) Wastewater-Based Epidemiology to Describe the Evolution of SARS-CoV-2 in the South-East of Spain, and Application of Phylogenetic Analysis and a Machine Learning ApproachVirusesJuly 3, 2023Jose A. Férez, Enric Cuevas-Ferrando, María Ayala-San Nicolás, Pedro J. Simón Andreu, Román López, Pilar Truchado, Gloria Sánchez, Ana Allendeto the loading cartridge, and 300 μL of lysis buffer was added. The cartridge was then loaded in the Maxwell® RSC Instrument (Promega, Madison, WI, USA) using the “Maxwell RSC Viral total Nucleic Acid” running program for the

(390) Salivary miRNA Profiles in COVID-19 Patients with Different Disease SeveritiesInternational Journal of Molecular SciencesJuly 1, 2023Irma Saulle, Micaela Garziano, Gioia Cappelletti, Fiona Limanaqi, Sergio Strizzi, Claudia Vanetti, Sergio Lo Caputo, Mariacristina Poliseno, Teresa Antonia Santantonio, Mario Clerici, et al.using the Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega, Fitchburg, WI, USA) with the Maxwell® RSC Instrument (Promega, Fitchburg, WI, USA), as previously described [51]. Briefly, single-step, real-time RT-qPCR

(391) TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseasesRedox biologyJuly 1, 2023Kim J, Lim J, Yoo ID, Park S, Moon JS.(AS1340, Promega), or Maxwell® RSC simplyRNA cells kit (AS1390, Promega) with Maxwell® RSC Instrument (AS4500, Promega). Amounts of RNA were measured with QuantiFluor® RNA System (E3310, Promega) using Quantus™ Fluorometer

(392) First Report of Olive Branch Dieback in Croatia Caused by Cytospora pruinosa DéfagoMicroorganismsJune 28, 2023Elena Petrović, Karolina Vrandečić, Dario Ivić, Jasenka Ćosić, Sara Godenamargins and used for genomic DNA extraction. Total genomic DNA from the isolate was extracted using a Maxwell® RSC Instrument (Promega, Madison, WI, USA) and Maxwell® RSC Plant DNA Kit (Promega, Madison, WI, USA). The nuclear

(393) Autosomal recessive hyposegmentation of granulocytes in Australian Shepherd Dogs indicates a role for LMBR1L in myeloid leukocytesPLOS GeneticsJune 22, 2023Bianca Lourdes Frehner, Matthias Christen, Iris M. Reichler, Vidhya Jagannathan, Marilisa Novacco, Barbara Riond, Laureen M. Peters, José Suárez Sánchez-Andrade, Aldona Pieńkowska-Schelling, Claude Schelling, et al.extraction Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit, using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Genomic DNA from liver tissue samples of the stillborn puppies was

(394) A Comparison of Two Different FFPE Tissue Dissection Methods for Routine Diagnostics in Molecular Pathology: Manual Macrodissection versus Automated Microdissection Using the Roche “AVENIO Millisect” SystemCancers (Basel)June 20, 2023Jan Jeroch, Tobias Riedlinger, Christina Schmitt, Silvana Ebner, Ria Winkelmann, Peter J. Wild, Melanie Demesdissection methods was digested with proteinase K and the nucleic acid was extracted with the help of the MaxWell® RSC instrument (Promega, Madison, WI, USA) and the Maxwell® FFPE Plus LEV DNA Purification Kit (Promega, Madison, WI,

(395) Prevalence of Enterococcus spp. and the Whole-Genome Characteristics of Enterococcus faecium and Enterococcus faecalis Strains Isolated from Free-Living Birds in PolandPathogensJune 16, 2023Renata Kwit, Magdalena Zając, Aleksandra Śmiałowska-Węglińska, Magdalena Skarżyńska, Arkadiusz Bomba, Anna Lalak, Ewelina Skrzypiec, Dominika Wojdat, Weronika Koza, Emilia Mikos-Wojewoda, et al.Cultured Cells (AS1620 Promega, Madison, WI, USA), according to the manufacturer’s instructions, with a Maxwell® RSC Instrument (Promega, Madison, WI, USA). Sequencing libraries were prepared with KAPA HyperPlus (Roche, Pleasanton,

(396) Detection of circulating cell-free HPV DNA of 13 HPV types for patients with cervical cancer as potential biomarker to monitor therapy response and to detect relapseBritish Journal of CancerJune 15, 2023Suzana Mittelstadt, Olga Kelemen, Jakob Admard, Axel Gschwind, André Koch, Sarah Wörz, Ernst Oberlechner, Tobias Engler, Irina Bonzheim, Annette Staebler, et al.necessary. Genomic DNA was extracted from the sections using the Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Next-Generation Sequencing

(397) Circulating miR-26b-5p and miR-451a as diagnostic biomarkers in medullary thyroid carcinoma patientsJournal of Endocrinological InvestigationJune 7, 2023Z. M. Besharat, S. Trocchianesi, A. Verrienti, R. Ciampi, S. Cantara, C. Romei, C. Sabato, T. M. R. Noviello, A. Po, A. Citarella, et al.pEV samples RNA was extracted using PROMEGA Maxwell RSC miRNA Plasma and Serum kit (#AS1680) by the Maxwell® RSC Instrument according to manufacturer’s instruction. Extracted RNA was eluted in 30 μl of H2O RNase-free. Prior

(398) Establishment of wastewater-based SARS-CoV-2 monitoring system over two years: Case studies in South KoreaJournal of Environmental Chemical EngineeringJune 5, 2023Lan Hee Kim, Viktorija Mikolaityte, Sungpyo Kimvortexed for 10 s. The extractants were incubated for 10 min at 56 °C, and the RNA was extracted using a Maxwell® RSC instrument (Promega, USA). For the sewage sludge samples, the 0.5 g of sludge was placed in a 1.5 mL microtube

(399) Epidemiology and zoonotic transmission of mcr-positive and carbapenemase-producing Enterobacterales on German turkey farmsFrontiers in MicrobiologyJune 2, 2023Katja Nordhoff, Martina Scharlach, Natalie Effelsberg, Carolin Knorr, Dagmar Rocker, Katja Claussen, Richard Egelkamp, Alexander C. Mellmann, Andreas Moss, Ilona Müller, et al.these cultures were used for DNA extraction. Genomic DNA of E. coli isolates was extracted with the Maxwell® RSC Instrument (an automated nucleic acid purification platform) using the Maxwell® RSC Whole Blood DNA Kit (Promega,

(400) IPSC reprogramming of two patients with spondyloepiphyseal dysplasia congenita (SEDC)Stem Cell ResearchJune 1, 2023Pauline De Kinderen, Laura Rabaut, Melanie H.A.M. Perik, Silke Peeters, Peter Ponsaerts, Bart Loeys, Geert Mortier, Josephina A.N. Meester, Aline Verstraetenanalysis) Genomic DNA of patient PBMCs and the resulting iPSCs (passage 12) was isolated using the Maxwell® RSC Instrument and Maxwell® RSC Cultured Cells DNA Kit (Promega). Subsequently, a HumanCytoSNP-12 assay (Illumina)

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