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Protein Biochemistry
Western Blot Analysis
Western Blotting Reagents
Substrates
Substrates

Substrates

Substrates for applications in immunohistochemistry, immunoassays, Western blotting, and functional assays are available from a number of reputable suppliers. Among these substrates are for common antibody conjugates horseradish peroxidase (HRP) and alkaline phosphatase (AP). These include ECL, TMB, DAB, and pNPP, BCIP/NBT, respectively. Enzyme substrates for reporter assays are also available, such as luciferin for luciferase assays, and X-Gal, X-Gluc, and ONPG for beta-galactosidase. Filter for desired attributes on the left to help narrow down your substrate search.

Substrates

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User Reviews (17)
Figures (20)

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Your search returned 1833 Substrates across 46 suppliers.

Showing 6 of 46 suppliers (1833 products total)

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Creative Peptides

Bremelanotide Acetate

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Desmopressin Acetate Trihydrate

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Creative Diagnostics

TMB Liquid Substrate System

Quantity:
100 ml, 1 L
Detection Method:
Colorimetric
Format:
Liquid

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AssayPro

Chromogen Substrate (TMB)

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Applications:
Assay

Supplier Page$50.00

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Chromogen Substrate (TMB)

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Applications:
Assay

Supplier Page$10.00

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Cell Signaling Technology

20X LumiGLO ® Reagent and 20X Peroxide

Quantity:
50 ml
Applications:
Western Blot

Citations:

(160)

$259.00

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SignalStain ® Boost IHC Detection Reagent (AP, Mouse)

Quantity:
15 ml
Applications:
Immunohistochemistry, Immunohistochemistry - fixed

Citations:

(5)

$339.00

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New England Biolabs

BG-GLA-NHS

Quantity:
2 mg
Applications:
Protein Labeling, Other
Format:
Solid

Citations:

(35)

$451.00

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BG-Maleimide

Quantity:
2 mg
Applications:
Protein Labeling, Other
Format:
Solid

Citations:

(2)

$444.00

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Creative Bioarray

AEC Solution (IHC) (601 Slides)

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AEC substrate

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Showing 6 of 46 suppliers (1833 products total)

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New England Biolabs
10/21/2024Excellent SnapTag Ligand for Labelling Protein-SnapTag Fusions for Confocal Imaging Studies Pavan Kumar ChodisettiTo understand the role of a cell surface protein in cell morphogenesis during the ... Read Review
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New England Biolabs
8/23/2023Highly Sensitive SNAP-tag Ligand: SNAP-Cell® TMR-Star From New England Biolabs Pavan Kumar ChodisettiI am studying the biology of a cell division protein in bacteria. As part of the study, I ... Read Review
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Promega
6/13/2023Stable Substrate for In Vivo Imaging Using IVIS Yao WangWe used this kit to tracking luciferase activity in a time-dependent manner in vivo. The ... Read Review
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Bio-Rad
2/8/2023Bio-Rad's ECL Reagent Kit for Western Blot Wei Yang SitThe Clarity Western ECL Substrate is used with any HRP-conjugated secondary antibody for ... Read Review
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Promega
8/16/2022Substrate Lights Up Experiments Tobias BeiglI was looking for a cheap Renilla luciferase substrate with a optimal price-to-performance... Read Review
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Bio-Rad
7/8/2020A Good Developing Reagent for Western Blotting sndp kmrGood and clear cut band of desired molecular weight is obtained upon addition of the ... Read Review
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Bio-Rad
5/20/2020Very Sensitive ECL Substrate for Film-Based Western Blot Hyo AhnThis is a good item for western blotting. However, I switched to fluorescent dye ... Read Review
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Bio-Rad
4/13/2020Sensitive and Stable ECL Substrate from Bio-Rad Elva ZhangDefinitely recommended for western blot experiments.
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Tocris Bioscience, a Bio-Techne Brand
7/10/2019MTT as a Tool to Estimate Cell Proliferation Peter Frankl.MTT can be used to investigate cell metabolism and viability.
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Promega
1/17/2019Excellent Kit for In Vivo Analysis Wei Yang SitCancer cells are transfected with the plasmid that encodes for the enzyme luciferase. ... Read Review
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Bio-Rad
9/6/2017Clarity Western ECL Substrate For Developing The HRP Conjugate Immunoblot Gajendra ReddyTo check the expression levels of protein of interest in different in-vitro experimental ... Read Review
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Advansta
5/10/2017WesternBright ECL HRP SubstrateDr. Elena DeryuginaWesternBright ECL HRP substrate from Advansta was used to detect by western blotting a ... Read Review
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R&D Systems, a Bio-Techne Brand
1/27/2016ELISA Substrate Kit Kai YThis kit is used as HRP substrate in ELISA assays. It produces signals detectable on a ... Read Review
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Advansta
8/26/2015WesternBright Quantum HRP Substrate Reagent Evan HermelThe replacement of Xray film with digital imaging requires high quality substrates for ... Read Review
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Bio-Rad
8/20/2015Clarity Western Blotting ECL Substrate Evan HermelWe have had difficulties getting good signal on our Licor digital western blot imager, ... Read Review
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Advansta
1/14/2015Evaluation of WesternBright ECL sample pack Lakshmi BolluI came to know about this product at the Annual Meeting of the American Association of ... Read Review
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Advansta
4/8/2014Detection of Low Abundant Proteins Song ChenWe are interested in understanding the regulation of protein stability of those important ... Read Review

BPS Bioscience, Inc.

NAD+, Biotin-Labeled

80610 | 500 µL

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1 Figure from 3 Publications

Bio-Rad

Clarity Max™ Western ECL Substrate, 100 ml

1705062

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5 Figures from 634 Publications

Bio-Rad

Clarity Western ECL Substrate, 500 ml

1705061

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1 Figure from 1723 Publications

Bio-Rad

Clarity™ and Clarity Max™ Western ECL Blotting Substrates

1705060S / 1705060 / 1705061 / 1705062S / 1705062 | EA

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3 Figures from 3430 Publications

Cytiva Life Sciences

Amersham™ ECL™ Prime Western blot detection reagent, for 1000 cm² membrane

RPN2232 | For 1000 cm² membrane

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1 Figure from 1290 Publications

Cytiva Life Sciences

Amersham™ ECL™ Western blot detection reagent, for 1 000cm² membrane

RPN2109 | For 1 000cm² membrane

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2 Figures from 196 Publications

Promega

Beetle Luciferin, Potassium Salt

E1605 | 1g

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2 Figures from 116 Publications

R&D Systems, a Bio-Techne Brand

Mca-KPLGL-Dpa-AR-NH2 Fluorogenic Peptide Substrate

ES010 | 1 mg

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1 Figure from 45 Publications

R&D Systems, a Bio-Techne Brand

Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate

ES003 | 1 mg

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1 Figure from 37 Publications

New England Biolabs

SNAP-Cell TMR-STAR

S9105S | 30 nmol

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1 Figure from 84 Publications

New England Biolabs

SNAP-Surface 549

S9112S | 50 nmol

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1 Figure from 36 Publications

New England Biolabs

SNAP-Surface Alexa Fluor 488

S9129S | 50 nmol

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1 Figure from 31 Publications

Showing 12 of 12 products

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Antibody Labeling
Cell Culture
Cell Isolation
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Destaining
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Antibody Diluents
Antigen Retrieval Buffers
Bioink
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Collagen
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Diluents
Dissociation Reagent
DNA Loading Dyes
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Wash Buffers

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Liquid
Solid

Substrate

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ABTS
AEC
Amplex Red
BCIP/NBT
DAB
ECL
Luciferin
Peroxidase Substrate
Phosphatase Substrate
pNPP
Tetrazolium Salts (MTT/XTT/MTS/WST)
TMB
X-Gluc
Alkaline Phosphatase Substrate
Caspase Substrate
Fast Red
HRP Substrate
Luciferase Substrate
ONPG
X-Gal

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DNA Stain
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Coomassie Stain
Ponceau S
Silver Stain
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Hematoxylin

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Chemiluminescent
Colorimetric
Fluorometric
Luminescent

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Agarose
APC
Biotin
FITC
Gold
Horseradish Peroxidase
Phycoerythrin (PE)

Companies

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AAA Biotech, LLC
AAT Bioquest, Inc.
Abcam
Advansta
Agrisera
Aladdin Scientific
AssayPro
Aviva Systems Biology
Bethyl Laboratories, Inc.
BioLegend
Biomatik
Bio-Rad
Biorbyt
Biotium, Inc.
BOC Sciences
BosterBio
BPS Bioscience, Inc.
Cayman Chemical
Cell Signaling Technology
Creative Bioarray
Creative Biolabs
Creative Diagnostics
Creative Enzymes
Creative Peptides
Cytiva Life Sciences
Enzo Life Sciences, Inc.
Fine Biotech Co., Ltd.
Fisher BioReagents
G Biosciences
Hzymes Biotechnology Co., Ltd.
ImmunoChemistry Technologies
Leinco Technologies, Inc.
Mabtech AB
MedChemExpress
MuseChem
MyBioSource.com
Neogen Corporation
New England Biolabs
Novus Biologicals, a Bio-Techne Brand
Promega
R&D Systems, a Bio-Techne Brand
RayBiotech
Revvity
Rockland Immunochemicals, Inc.
Santa Cruz Biotechnology, Inc.
Tocris Bioscience, a Bio-Techne Brand

Reviews

Excellent SnapTag Ligand for Labelling Protein-SnapTag Fusions for Confocal Imaging Studies
To understand the role of a cell surface protein in cell morphogenesis during the bacterial cell ... Read Review
Highly Sensitive SNAP-tag Ligand: SNAP-Cell® TMR-Star From New England Biolabs
I am studying the biology of a cell division protein in bacteria. As part of the study, I intend to ... Read Review
Highly Sensitive ECL Western Blotting Substrate From ThermoFisher
As a protein biochemist studying protein levels and protein-protein interactions, Western blotting ... Read Review

Substrates

Substrates

Creative Peptides

Bremelanotide Acetate

Desmopressin Acetate Trihydrate

Creative Diagnostics

TMB Liquid Substrate System

AssayPro

Chromogen Substrate (TMB)

Chromogen Substrate (TMB)

Cell Signaling Technology

20X LumiGLO ® Reagent and 20X Peroxide

SignalStain ® Boost IHC Detection Reagent (AP, Mouse)

New England Biolabs

BG-GLA-NHS

BG-Maleimide

Creative Bioarray

AEC Solution (IHC) (601 Slides)

AEC substrate

Substrates

Selected Filters

Citations / Reviews

Applications

Reagent Type

Format

Substrate

Stain Type

Detection Method

Conjugate/Tag

Companies

Reviews

Excellent SnapTag Ligand for Labelling Protein-SnapTag Fusions for Confocal Imaging Studies

Highly Sensitive SNAP-tag Ligand: SNAP-Cell® TMR-Star From New England Biolabs

Highly Sensitive ECL Western Blotting Substrate From ThermoFisher

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