Chemical substrates are available for a variety of applications, including immunohistochemistry, immunoassays, Western blotting, and functional assays. Substrates for the common antibody labels horseradish peroxidase (HRP) and alkaline phosphatase (AP) include ECL, TMB, DAB, ABTS, AEC, and pNPP, BCIP/NBT and Fast Red, respectively. Upon reacting with the enzyme labels, these substrates enable visualization or measurement of target proteins through visible color, fluorescence or chemiluminescence. Luciferin, among other luciferase substrates, is a key component of luciferase reporter assays for gene expression. Substrates for beta-galactosidase, another type of reporter, include X-Gal, X-Gluc, and ONPG. Tetrazolium salts (MTT, XTT, MTS, WST) are dehydrogenase substrates commonly used in cell-based assays for detecting live, actively metabolizing cells. Caspase substrates are largely short, fluorescently-labeled peptides that enable visualization of caspase activity. The Biocompare substrates search tool aims to simplify the browsing for various substrates used in biomedical research.
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The benefits, challenges, and future directions of this major advance.
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Set up a live demo with a Bio-Rad Imaging Specialist - Request a DemoUse the ChemiDoc MP Imaging System for flexible, high-sensitivity multiplex fluorescent and chemiluminescence western blot detection, imaging gels, analysis and documentation. This ...
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Measure and assess your cell cultures with ease. Our Millicell® DCI Digital Cell Imager enables more efficient execution of the repetitive daily techniques associated with cell passaging. Quickly and objectively assess confluency, morphology, ...
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We used regularly our protein assays and analysis, protein biology in western bottling. very small volume for A and B solution perfect enhancer.
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We used this kit to tracking luciferase activity in a time-dependent manner in vivo. The kit gave us clear and stable results through our experiments.
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