Chemical substrates are available for a variety of applications, including immunohistochemistry, immunoassays, Western blotting, and functional assays. Substrates for the common antibody labels horseradish peroxidase (HRP) and alkaline phosphatase (AP) include ECL, TMB, DAB, ABTS, AEC, and pNPP, BCIP/NBT and Fast Red, respectively. Upon reacting with the enzyme labels, these substrates enable visualization or measurement of target proteins through visible color, fluorescence or chemiluminescence. Luciferin, among other luciferase substrates, is a key component of luciferase reporter assays for gene expression. Substrates for beta-galactosidase, another type of reporter, include X-Gal, X-Gluc, and ONPG. Tetrazolium salts (MTT, XTT, MTS, WST) are dehydrogenase substrates commonly used in cell-based assays for detecting live, actively metabolizing cells. Caspase substrates are largely short, fluorescently-labeled peptides that enable visualization of caspase activity. The Biocompare substrates search tool aims to simplify the browsing for various substrates used in biomedical research.
Expert tips on ensuring accuracy and reproducibility
read more
New approaches overcome some of the most persistent challenges
read more
Measure and assess your cell cultures with ease. Our Millicell® DCI Digital Cell Imager enables more efficient execution of the repetitive daily techniques associated with cell passaging. Quickly and objectively assess confluency, morphology, ...
read more
The Sapphire FL Biomolecular Imager is a field-upgradable laser scanner, with a wide selection of user-adjustable lasers and filters. It features 5-micron resolution, adjustable Z-plane focus and a wide field of view of 25cm X 25cm. Applications ...
read more
We used regularly our protein assays and analysis, protein biology in western bottling. very small volume for A and B solution perfect enhancer.
read more
We used this kit to tracking luciferase activity in a time-dependent manner in vivo. The kit gave us clear and stable results through our experiments.
read more