New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

(1) Clathrin-independent endocytosis and retrograde transport in cancer cells tune immune synapse organization and CD8 T cell responseElifeApril 22, 2026Shiqiang Xu, Alix Buridant, Thibault Hirsch, Céline Duhamel, Benjamin Ledoux, Massiullah Shafaq-Zadah, Estelle Dransart, Louise Thines, Ludger Johannes, Pierre Van der Bruggen, et al.antibody,"Santa Cruz Biotechnology, sc-1178",AB_627074,IF,1:100 SNAP-Cell TMR-Star,"New England Biolabs, S9105S",/,IF,1:200 Anti-GM130 antibody,"BioLegend, 937002",AB_2888895,IF,1:200 Alexa Fluor 488 Phalloidin,"Invitrogen,

(2) Clathrin-independent endocytosis and retrograde transport in cancer cells tune immune synapse organization and CD8 T cell responseeLifeApril 22, 2026Shiqiang Xu, Alix Buridant, Thibault Hirsch, Céline Duhamel, Benjamin Ledoux, Massiullah Shafaq-Zadah, Estelle Dransart, Louise Thines, Ludger Johannes, Pierre Van Der Bruggen, et al.antibody,"Santa Cruz Biotechnology, sc-1178",AB_627074,IF,1:100 SNAP-Cell TMR-Star,"New England Biolabs, S9105S",/,IF,1:200 Anti-GM130 antibody,"BioLegend, 937002",AB_2888895,IF,1:200 Alexa Fluor 488 Phalloidin,"Invitrogen,

(3) Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargoiScienceMarch 20, 2026Evelyn R. Smith, Emma D. Turner, Mahmoud A.S. Abdelhamid, Timothy D. Craggs, Alison E. Twelvetrees,New England Biolabs,Cat#S9102S SNAP-Surface Alexa Fluor 488,New England Biolabs,Cat#S9129S SNAP-Cell TMR-Star,New England Biolabs,Cat#S9105S Dulbecco’s Modified Eagle Medium (DMEM) - high glucose,Sigma-Aldrich,Cat#D5796 Fetal Bovine Serum

(4) FidlTrack: high-fidelity structure-aware single particle tracking resolves intracellular molecular motion in organelles sensing APP processingNature CommunicationsFebruary 11, 2026Pierre Parutto, Yutong Yuan, Valentina Davì, Roger Pons-Lanau, Svenja Ebeling, Karnika Gupta, Francesca Bottanelli, Maria F. Garcia-Parajo, Felix Campelo, Clemens F. Kaminski, et al.density on a glass coverslip and stained overnight with Halo-JFX650 (25 µM) and with Snap TMR (NEB #S9105S, 500 µM) for 1 h. Following TMR staining, the cells were washed 2x with PBS (Gibco #14190136), left

(5) Quantitative optical nanoscopy of mitochondrial-derived vesicles in neurons classifies pre-peroxisomal and clearing organellesNature CommunicationsJanuary 8, 2026Giovanna Coceano, Jonatan Alvelid, Martina Damenti, Gabriella Ferretti, Johannes Mueller, Joanna Rorbach, Ilaria Testa18, 20 and 24 h after transfection (T = 0) cells were loaded with SNAP-Cell® TMR-Star ligand (NEB, S9105S) for 1 h at 3 μM; subsequently washed 3 times in ACSF; let equilibrate in fresh media for 1 h; fixed

(6) Dual pathways via CENP-C and Mis18C recruit HJURP for CENP-A deposition into vertebrate centromeresThe EMBO JournalJanuary 8, 2026Tetsuya Hori, Yutaka Mahana, Mariko Ariyoshi, Tatsuo Fukagawa090-07123 SNAP-Cell Block,New England Biolabs,Cat#: S9106S SNAP-Cell TMR-Star,New England Biolabs,Cat#: S9105S Dynabeads protein G,Thermo Fisher,Cat#: 10009D NP-40 alternative,ChemCruz,Cat#: sc-281108 BSA,Equitech-Bio

(7) Rate-limiting enzymes in nucleotide metabolism synchronize nucleotide biosynthesis and chromatin formationMolecular CellDecember 18, 2025Shashank Srivastava, Daniela Samaniego-Castruita, Shubhi Srivastava, Sakshi Khurana, Jalees Rehman, Vipul Shukla, Issam Ben-Sahra, Daniel R. Foltzsynthesis of new SNAP-tagged histones and chased for 2 hours. 2 μM SNAP-Cell TMR Star (New England Biolabs S9105S) was added to cells for 30 minutes to label new SNAP-tagged histones. Cells were washed twice with 1x-PBS

(8) Super-resolution microscopy reveals a Rab6a-dependent trafficking hub for rhodopsin at the mammalian rod photoreceptor GolgiBiology openNovember 15, 2025Hekmatara M, Thompson SL, Haggerty KN, Hagen S, Brothers BA, Daniels B, Luxardi G, Moshiri A, Deng WT, Robichaux MA.SNAP-Surface Alexa Fluor 647 [New England Biolabs (NEB), Cat# S9136S], SNAP-Cell® TMR-Star (NEB, Cat# S9105S) and DAPI (Thermo Fisher Scientific, Cat# 62248). DNA plasmids The bovine pcDNA3.1-Rho-EGFP-ETSQVAPA

(9) Spatially-resolved Photoproximity Profiling of MYC Identifies a MYC-BAF Liability in Cancer CellsbioRxivOctober 23, 2025Anthony J. Carlos, Shuyuan Huang, Dongbo Yang, Colin Swenson, Pratyasha Chakraborty, Shaopeng Yu, Benjamin D. Stein, Raymond E. Moellering(Gibco) 48 hours before the experiment. The cells were then treated with 3 μM of SNAP-cell TMR-STAR (NEB #S9105S) in 50uL of full media and incubated for 0.5 hr at 37°C. Cells were washing in 50 μL of full media 5

(10) Degradation of CaMKII is stimulated by its active conformationJournal of Biological ChemistryOctober 1, 2025Christina Quasney, Leslie C. Griffithwere expressed in HEK293T cells for 40 h. Cells were labeled with 1 μM TMR-Star (New England Biolabs, #S9105S) for 30 min at 37 °C, followed by three washes (15 min each) with 10 ml fresh media and a final 30-min

(11) Chemogenetic tuning reveals optimal MAPK signaling for cell-fate programmingCell ReportsSeptember 23, 2025Brittany A. Lende-Dorn, Jane C. Atkinson, Yunbeen Bae, Kate E. Gallowayconditions expressing SNAP-p53DD were stained using a fluorescent SNAP substrate (New England Biolabs, #S9105S), then all conditions were dissociated with trypsin. Cells were resuspended in N3 media and kept on

(12) Pathogenic KIF1A R350 Variants Disrupt A Conserved Kinesin-Tubulin Salt BridgebioRxivSeptember 11, 2025Abhipsa Shatarupa, Lu Rao, Ana B. Asenjo, Arne Gennerich, Hernando Sosaproteins with a SNAPf-tag, the resin was mixed with 10 μM SNAP-Cell® TMR-Star (New England Biolabs Inc., #S9105S) at room temperature for 10 minutes to label the SNAPf-tag. The resin was further washed with WB, and

(13) O-GlcNAcylation promotes angiogenic transdifferentiation to reverse vascular ischemiaNature Cardiovascular ResearchJuly 4, 2025Shuang Li, Alexander J. Lu, Eric S. Nagueh, Yanqiang Li, Michael Graber, Kaylee N. Carter, Elisa Morales, Crystina L. Kriss, Kaifu Chen, Junchen Liu, et al.OSMI-4. Then, the SNAP-tagged H3.3 was pulse-labeled with SNAP-Cell TMR-Star (tetramethylrhodamine) (S9105S, New England Biolabs) for 20 minutes followed by 30 minutes cell culture media wash and further cytoskeletal

(14) Distinct Clinical Phenotypes in KIF1A-Associated Neurological Disorders Result from Different Amino Acid Substitutions at the Same Residue in KIF1ABiomoleculesMay 2, 2025Lu Rao, Wenxing Li, Yufeng Shen, Wendy K. Chung, Arne Gennerich(w/v) Pluronic F-127, 10% glycerol). To label the SNAPf-tag or HaloTag, SNAP-Cell® TMR-Star ligand (#S9105S, New England Biolabs, Ipswich, MA, USA)—and HaloTag® Alexa Fluor® 660 Ligand (#G8472, Promega, Madison,

(15) Compact transcription factor cassettes generate functional, engraftable motor neurons by direct conversionCell SystemsApril 16, 2025Nathan B. Wang, Honour O. Adewumi, Brittany A. Lende-Dorn, Adam M. Beitz, Timothy M. O’Shea, Kate E. GallowayBovine Serum Albumin,Sigma-Aldrich,Cat#A2058 Laminin,Corning,Cat#354232 Gelatin,Sigma-Aldrich,Cat#G1890 Lenti-X Concentrator,Takara Bio,Cat#631232 SNAP-Cell 647-SiR,New England Biolabs (NEB),Cat#S9105S CellAdhere Laminin-521,STEMCELL Technologies,Cat#200-0117 Poly(ethyleneimine),Sigma-Aldrich,Cat#P3143 BrainPhys Neuronal Medium

(16) Portimine A toxin causes skin inflammation through ZAKα-dependent NLRP1 inflammasome activationEMBO Molecular MedicineFebruary 13, 2025Léana Gorse, Loïc Plessis, Stephen Wearne, Margaux Paradis, Miriam Pinilla, Rae Chua, Seong Soo Lim, Elena Pelluz, Gee-Ann TOH, Raoul Mazars, et al.al, 2020","Yang et al, 2020" Emricasan 5 µM,MedChem Express (MCE),HY-10396 SNAP-Cell TMR-Star,NEB,S9105S Human IL-1 Family Cytokine Array C1,Tebu-bio,AAH-IL1F-1–8 Human Inflammation Antibody Array,Abcam,ab134003 IL-1

(17) Identification of modulators of the ALT pathway through a native FISH-based optical screenCell reportsJanuary 28, 2025Azeroglu B, Khurana S, Wang SC, Tricola GM, Sharma S, Jubelin C, Cortolezzis Y, Pegoraro G, Miller KM, Stracker TH, et al.Block,NEB,Cat#S9106S SuperScript II Reverse Transcriptase,ThermoFisher,Cat# 18064014 TMR (tetramethylrhodamine) star,NEB,Cat#S9105S Triton X-100,Sigma,Cat#SIG-X100 Triptolide (RNAPIIi),Tocris,Cat # 3253 Experimental models: Cell

(18) G-quadruplex stabilization provokes DNA breaks in human PKD1, revealing a second hit mechanism for ADPKDNat CommunJanuary 2, 2025Agata M. Parsons, Seth Byrne, Jesse Kooistra, John Dewey, Aaron L. Zebolsky, Gloria Alvarado, Gerrit J. Bouma, Gregory B. Vanden Heuvel, Erik D. Larsonmodifications; SNAP-tagged dCAS9, TMR-snap ligand, and sgRNA synthesis kit (New England Biolabs, #M0652, #S9105S, #E3322V) were used as reagents, and since tween disrupted tissue morphology PBS alone was use in washes.

(19) Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopyElifeNovember 8, 2024Mitsuhiro Abe, Masataka Yanagawa, Michio Hiroshima, Toshihide Kobayashi, Yasushi SakoBSA, cells were further incubated for 24 hr. Cells were stained with 10 nM SNAP-Cell TMR-Star ligand (#S9105S, New England Biolabs) and 1 nM SF650 HaloTag ligand for 30 min, and washed at least three times with

(20) Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopyeLifeNovember 8, 2024Mitsuhiro Abe, Masataka Yanagawa, Michio Hiroshima, Toshihide Kobayashi, Yasushi SakoBSA, cells were further incubated for 24 hr. Cells were stained with 10 nM SNAP-Cell TMR-Star ligand (#S9105S, New England Biolabs) and 1 nM SF650 HaloTag ligand for 30 min, and washed at least three times with

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8/23/2023Highly Sensitive SNAP-tag Ligand: SNAP-Cell® TMR-Star From New England Biolabs Pavan Kumar ChodisettiI am studying the biology of a cell division protein in bacteria. As part of the study, I intend to examine the location of the protein fused to SnapTag using a SnapTag ligand purchased from NEB.

SNAP-Cell TMR-STAR

SNAP-Cell TMR-STAR from New England Biolabs

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SNAP-Cell TMR-STAR from New England Biolabs

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