A Good Developing Reagent for Western Blotting

Osmania University
Department of Biochemistry
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Clarity Western ECL Substrate

Catalog Number:

170-5061

Western blotting is a gold standard technique for quantifying the protein of interest. The output of the technique lies in the sensitivity of the developing reagent used for developing the blot. Bio-Rad Clarity Western ECL Substrate provides us a reliable solution for developing the blot on a chemidoc instrument

Experimental Design and Results Summary

Application

Western Blotting

Starting Material

PVDF / nitrocellulose protein transferred blotting membrane

Tips

The reagent is stable for at least half an hour after mixing the two reagents

Results Summary

Good and clear cut band of desired molecular weight is obtained upon addition of the reagent to the blotting membrane

Image Gallery

Summary

The Good

Very reliable

The Bad

Nothing

The Bottom Line

I will definitely recommend this wonderful developing kit for your western blotting workflow.

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