Clarity Max™ Western ECL Substrate, 100 ml from Bio-Rad

Product Specs
ItemClarity Max™ Western ECL Substrate, 100 ml
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1705062
ConcentrationPrimary: 1:1,000–1:50,000;
Secondary: 1:50,000–1:250,000, Primary: 1:1,000–1:50,000;
Secondary: 1:50,000–1:250,000
DetectionDigital or film imaging
Brand/TrademarkLSG LSD

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Regulation of the apico-basolateral trafficking polarity of the homologous copper-ATPases ATP7A and ATP7BJournal of Cell ScienceMarch 1, 2024Ruturaj, Monalisa Mishra, Soumyendu Saha, Saptarshi Maji, Enrique Rodriguez-Boulan, Ryan Schreiner, Arnab Guptaovernight, followed by secondary antibody conjugated to HRP. Then, the membranes were developed (Bio-Rad #1705062) and visualized using ChemiDoc (Bio-Rad). Generation of AP1 knockout MDCK-II cell lines For AP-1 subunit

(2) Role of heat shock protein 70 in silibinin-induced apoptosis in bladder cancerJ CancerJanuary 1, 2024Yi Wei, Yanxin Zhuang, Yishuai Zhang, Lei Luo, Bixin Yu, Jin ZengCell Signaling Technology, Inc. (Beverly, MA). The enhanced chemiluminescence (ECL) detection system (cat. no. 1705062) was from Bio‑Rad Laboratories, Inc. (Hercules, CA). Cell Viability Assay Cell viability was assessed

(3) L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprintRNA BiologyDecember 31, 2023Rachael Anne Dunlop, Sandra Anne Banack, Paul Alan Coxmanufacturer’s instructions. Blots were developed using Clarity Max™ Western ECL Substrate (cat. no. 1705062, Bio-Rad Laboratories Hercules, CA, USA) and imaged on the BioRad ChemiDoc using the signal accumulation

(4) Overcoming the resistance of hepatocellular carcinoma to PD-1/PD-L1 inhibitor and the resultant immunosuppression by CD38 siRNA-loaded extracellular vesiclesOncoImmunologyDecember 31, 2023Jun Deng, Hui Kegoat anti-rabbit IgG (ab6721, 1:5000, Abcam) at ambient temperature for 1 h. Afterward, ECL reagent (1705062, Bio-Rad) was used to visualize the results by the Image Quant LAS 4000C Gel Imager. IHC Paraffin

(5) The Crucial Role of Single-Stranded DNA Binding in Enhancing Sensitivity to DNA-Damaging Agents for Schlafen 11 and Schlafen 13iScienceDecember 15, 2023Kohei Fujiwara, Masashi Maekawa, Yuki Iimori, Akane Ogawa, Takeshi Urano, Nobuaki Kono, Hiroyuki Takeda, Shigeki Higashiyama, Makoto Arita, Junko Muraiserum albumin (BSA),Wako,Cat#015-21274 Clarify Max Western ECL Substrate,Bio-Rad Laboratories,Cat#1705062 4% paraformaldehyde,Nacalai Tesque,Cat#09154-56 Mounting Medium with DAPI,Abcam,Cat#ab104139 ,, Critical

(6) Influence of Breast Cancer Extracellular Vesicles on Immune Cell Activation: A Pilot StudyBiology (Basel)December 15, 2023Jessie Santoro, Barbara Carrese, Maria Sara Peluso, Luigi Coppola, Massimiliano D'Aiuto, Gennaro Mossetti, Marco Salvatore, Giovanni Smaldone(Bio-Rad Laboratories, Cat. 12003154, Hercules, CA, USA) and Clarity Max™ Western ECL Substrate (Cat. 1705062, Bio-Rad Laboratories, Hercules, CA, USA). BT474 and HS578T monolayer cell lysates (CL) were used as

(7) Survivin as a mediator of stiffness-induced cell cycle progression and proliferation of vascular smooth muscle cellsAPL BioengineeringDecember 1, 2023John C. Biber, Andra Sullivan, Joseph A. Brazzo, Yuna Heo, Bat-Ider Tumenbayar, Amanda Krajnik, Kerry E. Poppenberg, Vincent M. Tutino, Su-Jin Heo, John Kolega, et al.1:5000). Antibody signals were detected using Clarity (Cat. No. 1705061, Bio-Rad) or Clarity Max (Cat. No. 1705062, Bio-Rad) Western ECL substrate. For immunostaining, VSMCs cultured on hydrogels were fixed in 3.7%

(8) Survivin regulates intracellular stiffness and extracellular matrix production in vascular smooth muscle cellsAPL bioengineeringDecember 1, 2023Krajnik A, Nimmer E, Brazzo JA 3rd, Biber JC, Drewes R, Tumenbayar BI, Sullivan A, Pham K, Krug A, Heo Y, et al.Clarity Western ECL substrate (Cat. No. 1705061, Bio-Rad) or Clarity Max Western ECL substrate (Cat. No. 1705062, Bio-Rad) were used for antibody detection. F. Immunostaining hVSMCs cultured on hydrogels for 24

(9) Genome-wide profiling of transcription factor activity in primary liver cancer using single-cell ATAC sequencingCell ReportsNovember 28, 2023Amanda J. Craig, Maruhen A. Datsch Datsch Silveira, Lichun Ma, Mahler Revsine, Limin Wang, Sophia Heinrich, Zachary Rae, Allison Ruchinskas, Kimia Dadkhah, Whitney Do, et al.secondary antibodies for detection using luminescence (Clarity Max Western ECL Blotting Substrates (Biorad #1705062). Images were captured using a Chemidoc MP Image System (Bio-Rad) and cropped using the Image Lab Software.

(10) Biophysical properties of NaV1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cellsScientific ReportsNovember 24, 2023Charles-Albert Chapotte-Baldacci, Marion Pierre, Mohammed Djemai, Valérie Pouliot, Mohamed Chahineantibodies. Proteins were revealed using Clarity and Clarity Max Western ECL substrates(Cat# 1705060 and Cat# 1705062, Bio-Rad) and were visualized using the ChemiDoc system (Bio-Rad, ON, Canada). Relative quantification

(11) RAR-Dependent and RAR-Independent RXR Signaling in Stem-like Glioma CellsInternational Journal of Molecular SciencesNovember 17, 2023Amanda Dabrock, Natalie Ernesti, Florian Will, Manaf Rana, Nadja Leinung, Phillip Ehrich, Volker Tronnier, Christina ZechelSubstrate (#34076; Thermo Fisher Scientific; Loughborough, UK) or Clarity Max Western ECL Substrate (#1705062, BioRad). Chemiluminescence was detected with the ChemiDoc XRS system (BioRad), and quantification was

(12) The transcription factor ELF4 alleviates inflammatory bowel disease by activating IL1RN transcription, suppressing inflammatory TH17 cell activity, and inducing macrophage M2 polarizationFrontiers in ImmunologyNovember 6, 2023Meiwan Cao, Peiyu Chen, Baoling Peng, Yang Cheng, Jing Xie, Ziang Hou, Huan Chen, Liping Ye, Huiwen Li, Hongli Wang, et al.washing with 1×TBST buffer for 3 times, 5 minutes each time. The membrane was immersed in ECL reagent (1705062, Bio-Rad, USA) and exposed at room temperature for 1 minute. The liquid was removed, the membrane was

(13) IFNɣ but not IFNα increases recognition of insulin defective ribosomal product-derived antigen to amplify islet autoimmunityDiabetologiaNovember 1, 2023Sofia Thomaidou, Amadeo Munoz Garcia, Sabine de Lange, Jin Gan, Arno R. van der Slik, Rob C. Hoeben, Bart O. Roep, Françoise Carlotti, Arnaud ZaldumbideSanta Cruz Biotechnology and were used at a dilution of 1:5000. Western ECL substrate was used for imaging (1705062, BioRad, USA). Pancreatic islet treatment Pancreatic islets used in the T cell co-culture assays were

(14) Loss of Pla2r1 decreases cellular senescence and age‐related alterations caused by aging and Western dietsAging CellNovember 1, 2023Amélie Massemin, Delphine Goehrig, Jean‐Michel Flaman, Sara Jaber, Audrey Griveau, Sophia Djebali, Elisabeth Marcos, Léa Payen, Jacqueline Marvel, Romain Parent, et al.(715‐035‐152, Interchim) 1 h at room temperature. Detection was performed using an ECL kit either from Biorad (#1705062) or from ThermoScientific (#32201). 4.9 Transcriptomic analysis Transcriptome analysis of liver tissue

(15) Palmitate and glucose increase amyloid precursor protein in extracellular vesicles: Missing link between metabolic syndrome and Alzheimer's diseaseJournal of Extracellular VesiclesNovember 1, 2023Bhumsoo Kim, Yoon‐Tae Kang, Faye E. Mendelson, John M. Hayes, Masha G. Savelieff, Sunitha Nagrath, Eva L. FeldmanClarity Western ECL (catalog # 1705061, BioRad, Hercules, CA) or Calrity Max Western ECL (catalog # 1705062, BioRad), depending on the signal strength. Images were captured using the ChemiDoc Imaging System and

(16) Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosisFrontiers in PharmacologyOctober 31, 2023Yujia Ye, Lihong Yang, Min Leng, Qian Wang, Jiankui Wu, Wen Wan, Huawei Wang, Longjun Li, Yunzhu Peng, Shengjie Chai, et al.Shanghai, China) secondary antibody (1:5,000). The bands were visualized using an ECL Substrate (Cat No. 1705062; Bio-Rad, Hercules, CA, United States) and a Tanon-5200 Chemiluminescent Imaging System (Tanon Science

(17) [SWI/SNF Complex Gene Mutations Promote the Liver Metastasis of Non-small Cell Lung Cancer Cells in NSI Mice]Zhongguo Fei Ai Za ZhiOctober 20, 2023Lingling Gao, Zhi Xie, Shouheng Lin, Zhiyi Lv, Wenbin Zhou, Ji Chen, Linlin Zhu, Li Zhang, Penghui Zeng, Xiaodan Huang, et al.54135）。TBST洗膜，室温孵育二抗1 h：Anti-rabbit IgG（1:2000, CST, 7074），使用Clarity Max Western ECL Substrate（BIO-RAD, 1705062）显影液显影，用ImageJ软件计算分析条带灰度值。 1.5 病毒包装和细胞转染示踪细胞模型构建使用293T细胞和Lipofectamine 2000（InvitrogenTM, 11668030）以及包装质粒体系：pWPXLd-luciferase-EGFP（中国科学院广州生物医药与健康研究院）9

(18) Plant-derived cell-penetrating microprotein α-astratide aM1 targets Akt signaling and alleviates insulin resistanceCellular and Molecular Life SciencesOctober 1, 2023Bamaprasad Dutta, Shining Loo, Antony Kam, James P. Tamantibodies were obtained from Cell Signalling Technology, USA. Clarity Max™ Western ECL blotting substrates (1705062) were purchased from Bio-Rad Laboratories, Italy. The cell line C2C12 (ATCC CRL-1772), 3T3-L1 (ATCC

(19) Effects of low doses of methylmercury (MeHg) exposure on definitive endoderm cell differentiation in human embryonic stem cellsArchives of ToxicologyOctober 1, 2023Bai Li, Xiaolei Jin, Hing Man Chanfor three times with TBST and incubated with enhanced chemiluminescence substrate (ECL, Catalog no. 1705062; Bio-Rad) in the dark and imaged with the ChemiDoc XRS + imaging system. The Image Lab software (Bio-Rad)

(20) Multi-omics profiling reveals rhythmic liver function shaped by meal timingNature CommunicationsSeptember 29, 2023Rongfeng Huang, Jianghui Chen, Meiyu Zhou, Haoran Xin, Sin Man Lam, Xiaoqing Jiang, Jie Li, Fang Deng, Guanghou Shui, Zhihui Zhang, et al.(Cell Signaling Technology, Cat# 7074, RRID: AB_2099233, 1:5000), and enhanced chemiluminescent substrate (Bio-Rad #1705062) in the Azure C500 imaging system (see Fig. 7g and associated Source Data file). PVDF membranes were

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5/20/2020Very Sensitive ECL Substrate for Film-Based Western Blot Hyo AhnThis is a good item for western blotting. However, I switched to fluorescent dye conjugated secondary antibodies last year, which gives less sensitivity than HRP (side-by-side comparison), but more ... Read Review