SNAP-Surface Alexa Fluor 488 from New England Biolabs

Product Specs
ItemSNAP-Surface Alexa Fluor 488
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS9129S
Quantity50 nmol
ApplicationsCellular Biology
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) SIM imaging resolves endocytosis of SARS-CoV-2 spike RBD in living cellsCell chemical biologyMarch 16, 2023Miao L, Yan C, Chen Y, Zhou W, Zhou X, Qiao Q, Xu Z.Biological,Cat # M293-SUPI SNAP-Cy3,New England BioLabs,Cat # S9112S SNAP-Alexa 488,New England BioLabs,Cat # S9129S Lyso561,KeyGEN BioTECH,Cat # KGMP006 Bafilomycin A1,MedChemExpress,Cat # HY-100558 MCA-APK(Dnp)-OH,Beyotime,Cat

(2) Syndecan-2 selectively regulates VEGF-induced vascular permeabilityNat Cardiovasc ResMay 1, 2022Corti F, Ristori E, Rivera-Molina F, Toomre D, Zhang J, Mihailovic J, Zhuang ZW, Simons M.labeling, cells were incubated with 0.4 uM of SNAP-Surface Alexa Fluor 488 substrate (New England Biolabs, #S9129S) and a mouse anti-HA.11 (Covance, #MMS-101P, 1:200) diluted in starvation media for 30 minutes on ice

(3) Mobile origin-licensing factors confer resistance to conflicts with RNA polymeraseCell ReportsMarch 22, 2022Matthias J. Scherr, Syafiq Abd Wahab, Dirk Remus, Karl E. Duderstadtproteins","Chemicals, peptides, and recombinant proteins" ,, SNAP-Surface Alexa Fluor 488,New England Biolabs,Cat# S9129S LD555 maleimide mono-reactive dye,Lumidyne Technologies,Cat# LD555-MAL LD555-CoA,Lumidyne Technologies,Custom

(4) Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formationeLifeJanuary 20, 2022Lindsay B Case, Milagros De Pasquale, Lisa Henry, Michael K Rosen,New England BioLabs,Cat#S9132S, "Chemical compound, drug",SNAP-Surface Alexa Fluor 488,New England BioLabs,Cat#S9129S, "Chemical compound, drug",Intracellular pH Calibration Buffer Kit (Nigericin/Valinomycin),Invitrogen,Cat#P35379, "Software,

(5) Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neuronsNature communicationsNovember 18, 2021Bessa-Neto D, Beliu G, Kuhlemann A, Pecoraro V, Doose S, Retailleau N, Chevrier N, Perrais D, Sauer M, Choquet D.(#CLK-015-05) were purchased from Jena Bioscience (Jena, Germany). SNAP-Surface® Alexa Fluor® 488 (BG-AF488; #S9129S) was purchased from New England Biolabs. 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide

(6) Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formationJournal of Cell BiologyApril 5, 2021Ulrich Dobramysl et al.5–10 µM final protein concentration and 10 µM dye (SNAP-Surface Alexa Fluor488 [New England Biolabs; S9129S] or SNAP-Surface Alexa Fluor647 [New England Biolabs; S9136S], respectively) in 50 µl final volume with

(7) Combined kinesin-1 and kinesin-3 activity drives axonal trafficking of TrkB receptors in Rab6 carriersDevelopmental cellFebruary 22, 2021Zahavi EE, Hummel JJA, Han Y, Bar C, Stucchi R, Altelaar M, Hoogenraad CC.Heterodimerizer),Takara Bio,635056 Lipofectamine 2000,Thermo Fisher,11668019 Surface-SNAP-Alexa-488,NEB,S9129S Surface-SNAP-Alexa-546,NEB,S9132S Surface-SNAP-Alexa-647,NEB,S9136S SYLGARD 184 Silicone Elastomer

(8) N-glycosylation of α1D-adrenergic receptor N-terminal domain is required for correct trafficking, function, and biogenesisScientific ReportsDecember 1, 2020Eric M. Janezic, Sophia My-Linh Lauer, Robert George Williams, Michael Chungyoun, Kyung-Soon Lee, Edelmar Navaluna, Ho-Tak Lau, Shao-En Ong, Chris HagueTritonX-100/PBS for 1 min. Cells were incubated with 1 μM of SNAP Surface Alexa Fluor 488 (New England BioLabs #S9129S, Ipswich, MA) and 1:1000 ER Staining Kit-Red Fluorescence-Cytopainter (Abcam #139482, Cambridge, MA)

(9) Covalent Self-Labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis Seedlings[OPEN]The Plant CellOctober 1, 2020Ryu J. Iwatate et al.430,+,421,"444, 484",,Covalent labeling,"S9109S, NEB" SNAP-Surface Alexa Fluor 488,-,496,520,,covalent labeling,"S9129S, NEB" DRBG-488,n.d.a,496,520,,"covalent labeling, pulse chase labeling","(Komatsu et al., 2011)" SNAP-Cell TMR-Star

(10) A direct role for SNX9 in the biogenesis of filopodiaJournal of Cell BiologyApril 6, 2020Iris K. Jarsch et al.TOCA-1 and SNX9 were labeled using SNAP-Surface Alexa Fluor 488 or Alexa Fluor 647 (New England Biolabs, S9129S, S9136S). 5–10 µM final concentration of protein was mixed with 10 µM SNAP-dye in a buffer containing

(11) Kinesin-2 from C. reinhardtii Is an Atypically Fast and Auto-inhibited Motor that Is Activated by Heterotrimerization for Intraflagellar TransportCurrent BiologyMarch 5, 2020Punam Sonar, Wiphu Youyen, Augustine Cleetus, Pattipong Wisanpitayakorn, Sayed I. Mousavi, Willi L. Stepp, William O. Hancock, Erkan Tüzel, Zeynep Öktenbicarbonate,Sigma,Cat#A6141 Potassium acetate,Roth,Cat#HN10.3 SNAP-surface Alexa Fluor 488,New England Biolabs,Cat#S9129S SNAP-surface Alexa Fluor 647,New England Biolabs,Cat#S9136S EDTA-free protease inhibitor cocktail,Roche,Cat#4693132001 Tween-20,Sigma,Cat#P9416 Triton X-100

(12) A conserved molecular switch in Class F receptors regulates receptor activation and pathway selectionNature CommunicationsDecember 1, 2019Shane C. Wright et al.and subsequently cells were incubated with SNAP-substrate: either SNAP-Surface Alexa Fluor 488 (NEB #S9129S), SNAP-Surface Alexa Fluor 647 (NEB #S9136S) or SNAP-Cell 647-SiR (NEB #S9102S) at 1:200 dilution in

(13) Rad52 Restrains Resection at DNA Double-Strand Break Ends in YeastMolecular CellDecember 1, 2019Zhenxin Yan et al.(MMS),Sigma-Aldrich,M4016–1G Doxycycline hyclate,Sigma-Aldrich,D9891–5G SNAP-Surface Alexa Fluor 546,NEB,S9129S Chitin resin,NEB,S6651L cOmplete His-tag resin,Roche,5893682001 SP Sepharose,GE Healthcare,28950513 anti-FLAG-M2

(14) Stoichiometric Analyses of Soluble CD4 to Native-like HIV-1 Envelope by Single-Molecule Fluorescence SpectroscopyCell ReportsOctober 1, 2019Parul Agrawal, Anthony L. DeVico, James S. Foulke, George K. Lewis, Marzena Pazgier, Krishanu RayAlexa Fluor 647 Antibody Labeling Kit,Invitrogen,Catalog # A20186 SNAP-Surface® Alexa Fluor® 488,NEB,Catalog # S9129S Amicon® Ultra-15 Centrifugal Filter Unit,Sigma-Aldrich,Catalog # UFC901024 KEY RESOURCES TABLE

(15) rebuffregulates apical luminal matrix to control tube size inDrosophilatracheaBiology OpenSeptember 15, 2018Rachana R. Chandran, Aaron Scholl, Yuyang Yang, Lan Jiangresin. To label the purified chitin binding probe with SNAP tag, SNAP-surface Alexa Fluor 488 (NEB; cat# S9129S) was added and incubated for 1 h at 37°C. The fluorophore labeled probe was then dialyzed overnight

(16) Recruitment of two dyneins to an mRNA-dependent Bicaudal D transport complexeLifeJune 26, 2018Thomas E Sladewski, Neil Billington, M Yusuf Ali, Carol S Bookwalter, Hailong Lu, Elena B Krementsova, Trina A Schroer, Kathleen M Trybusconjugate,Invitrogen,Q10121MP, "Chemical compound, drug",SNAP-Surface Alexa Fluor 488,New England BioLabs,S9129S, "Chemical compound, drug",SNAP-Surface Alexa Fluor 647,New England BioLabs,S9136S, "Chemical compound,

(17) Cooperative Accumulation of Dynein-Dynactin at Microtubule Minus-Ends Drives Microtubule Network ReorganizationDevelopmental CellJanuary 18, 2018Ruensern Tan, Peter J. Foster, Daniel J. Needleman, Richard J. McKenneyProteins",, SNAP-Cell TMR-Star,NEB,S9105S SNAP-Surface Alexa Fluor 647,NEB,S9136S SNAP-Surface Alexa Fluor 488,NEB,S9129S Dylight 405 NHS Ester,ThermoFischer,46400 Alexa Fluor 647 NHS Ester,ThermoFischer,A37573 Biotinylated

(18) Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigenBMC BiotechnologyDecember 1, 2016Diana Klose, Ute Saunders, Stefan Barth, Rainer Fischer, Annett Marita Jacobi, Thomas NachreinerCatalogue number: S9136S) and SNAP-surface® Alexa Fluor® 488 (New England Biolabs; Catalogue number: S9129S) as previously described [17]. Briefly, 1 μg SNAP-TTC protein was mixed with 2 nmol BG-647 or BG-488

(19) Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the IntestineJournal of Biological ChemistryFebruary 11, 2011Lisa L. Maduzia, Evan Yu, Yinhua Zhangwith 1 mm DTT for 1 h at 37 °C. Fluorophores used for labeling were SNAP-surface Alexa Fluor 488 (NEB S9129S), SNAP-surface Alexa Fluor 546 (NEB S9132S) and SNAP-surface-IR800. After incubation, each labeled protein

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SNAP-Surface Alexa Fluor 488 from New England Biolabs

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SNAP-Surface Alexa Fluor 488 from New England Biolabs

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