pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(161) Cancer-associated mutations in VAV1 trigger variegated signaling outputs and T-cell lymphomagenesisThe EMBO journalNovember 15, 2021Robles-Valero J, Fernández-Nevado L, Lorenzo-Martín LF, Cuadrado M, Fernández-Pisonero I, Rodríguez-Fdez S, Astorga-Simón EN, Abad A, Caloto R, Bustelo XR.plasmids were obtained from Stratagene. The pRL‐SV40 plasmid was obtained from Promega (Cat. number E2231). The pGEX‐4T3 plasmid was a gift from C. Guerrero (Cancer Research Center, CSIC, Salamanca, Spain).

(162) ERK5 signalling pathway is a novel target of sorafenib: Implication in EGF biologyJournal of Cellular and Molecular MedicineNovember 1, 2021Marta Ortega‐Muelas et al.RRID:Addgene_47548. 24 ; Plasmid pRL Renilla Luciferase Control Reporter Vector was obtained from Promega (E2231), and plasmid pGL3‐3XMEF2‐luc was a gift from Dr. Ron Prywes (Addgene plasmid #32967; http://n2t.net/addgene:32967;

(163) Biochemically deleterious human NFKB1 variants underlie an autosomal dominant form of common variable immunodeficiencyThe Journal of experimental medicineNovember 1, 2021Li J, Lei WT, Zhang P, Rapaport F, Seeleuthner Y, Lyu B, Asano T, Rosain J, Hammadi B, Zhang Y, et al.sites. The pGL4.32[luc2P/NF-κB-RE/Hygro] and pRL-SV40 vectors were obtained from Promega (E8491 and E2231, respectively). Site-directed mutagenesis was performed as previously described (Zheng et al., 2004). Cell culture

(164) N(6)-methyladenosine modification regulates ferroptosis through autophagy signaling pathway in hepatic stellate cellsRedox biologyNovember 1, 2021Shen M, Li Y, Wang Y, Shao J, Zhang F, Yin G, Chen A, Zhang Z, Zheng S.transfection efficiencies, the luciferase reporter vectors were transfected in cells together with phRL-null (E2231, Promega). 2.30 Calculations and statistics The matched controls was used for individual animal experiments

(165) ADAM17 mediates ectodomain shedding of the soluble VLDL receptor fragment in the retinal epitheliumJournal of Biological ChemistryOctober 1, 2021Xiang Ma, Yusuke Takahashi, Wenjing Wu, Wentao Liang, Jianglei Chen, Dibyendu Chakraborty, Yangxiong Li, Yanhong Du, Siribhinya Benyajati, Jian-Xing Maa luciferase reporter (Addgene; #12456) and a Renilla luciferase control reporter vector (Promega; #E2231) were cotransfected into ARPE-19 cells as described previously (43). TCF/β-catenin transcriptional activity

(166) A non-translational role of threonyl-tRNA synthetase in regulating JNK signaling during myogenic differentiationFASEB JOctober 1, 2021Chong Dai, Adriana Reyes-Ordoñez, Jae-Sung You, Jie Chenobtained from Addgene: pCMV5-MEKK4alpha (#12187) and M50 Super 8 × TOPFlash (#12456). pRL-SV40 (Cat #: E2231) was from Promega. 2.3 | Cell culture C2C12 myoblasts were a gift from S. Kaufman at the University

(167) Suppression of CD80 Expression by ICP22 Affects Herpes Simplex Virus Type 1 Replication and CD8⁺IFN-γ⁺ Infiltrates in the Eyes of Infected Mice but Not Latency ReactivationJournal of virologySeptember 9, 2021Matundan HH, Wang S, Jaggi U, Yu J, Ghiasi H.multiple-cloning sites to drive the expression of the luciferase reporter. pRL-SV40 (catalog number E2231; Promega), a Renilla luciferase reporter plasmid, was used as the cotransfected internal control to

(168) LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loopBMC Pulmonary MedicineSeptember 8, 2021Wen Huang, Xinxing Wang, Fubing Wu, Fanggui XuFOXP3 and LINC00520 promoter. The pRL Renilla Luciferase Control Reporter Vector (catalogue number: E2231; Promega) provides normalization for the pmirGLO and pGL3 plasmids. Cell transfection MiR-3611 mimics

(169) Phytomodulatory proteins isolated from Calotropis procera latex promote glycemic control by improving hepatic mitochondrial function in HepG2 cellsSaudi Pharmaceutical JournalSeptember 1, 2021Keciany Alves de Oliveira, Hygor Nunes AraÃºjo, Tanes Iamamura de Lima, AndrÃ© Gustavo Oliveira, Bianca Cristine Favero-Santos, Dimitrius Santiago P.S.F. GuimarÃ£es, Paula Alexandre de Freitas, Regina de Jesus das Neves, Renata Prado Vasconcelos, Marina Gabrielle GuimarÃ£es de Almeida, et al.transfected cells using LipofectamineTM 3000 (Invitrogen, Carlsbad, CA, USA) with 0.02 µg of pRL-SV40 (E2231; Promega), 0.2 µg pf pPPRE X3-TK-luc (1015; Dr. Bruce Spiegelman; Addgene). The amount of plasmid DNA

(170) When Two plus Two Is More than Four: Evidence for a Synergistic Effect of Fatty Acids on Peroxisome Proliferator—Activated Receptor Activity in a Bovine Hepatic ModelGenes (Basel)August 21, 2021Sebastiano Busato, Massimo BionazPPRE X3-TK-luc (Addgene plasmid # 1015) was used as a measure of PPAR activation, while pRL-TK (cat# E2231, Promega, Madison, WI, USA), encoding Renilla reniformis guided by a mini-TK promoter, was used as to

(171) Development of Dl1.72, a Novel Anti-DLL1 Antibody with Anti-Tumor Efficacy against Estrogen Receptor-Positive Breast CancerCancers (Basel)August 13, 2021Gabriela Silva et al.#41726) along with 10 ng of pRL-SV40 vector encoding renilla luciferase (Promega, Madison, WI, USA, #E2231) using GeneJuice transfection reagent (Merck-Millipore, Darmstadt, Germany, #70967), according to the

(172) E2F1 and epigenetic modifiers orchestrate breast cancer progression by regulating oxygen-dependent ESRP1 expressionOncogenesisAugust 6, 2021Cheemala Ashok, Neha Ahuja, Subhashis Natua, Jharna Mishra, Atul Samaiya, Sanjeev Shukladifferent ESRP1 promoter–luciferase constructs along with pRL-TK Renilla luciferase plasmid (Promega, E2231). After 48 h transfection, cells were harvested and lysed and then subjected to detection of luciferase

(173) Revealing molecular pathways for cancer cell fitness through a genetic screen of the cancer translatomeCell reportsJune 29, 2021Kuzuoglu-Ozturk D, Hu Z, Rama M, Devericks E, Weiss J, Chiang GG, Worland ST, Brenner SE, Goodarzi H, Gilbert LA, et al.#12259 pLKO.1,"Stewart et al., 2003",Addgene #8453 pMSCV,Clontech,634401 pGL3,Promega,E1751 pRL,Promega,E2231 pLG15,Luke Gilbert, sgRNA expressing vectors,This paper, pMSCV-GFP-Tfeb,This paper, pMSCV-GFP-Pmpcb,This

(174) Cis-acting regulatory elements regulating CYP3A4 transcription in human liverPharmacogenetics and GenomicsJune 28, 2021Joseph M. Collins, Danxin WangAnalytical Science, Waltham, MA, USA). As an internal control, Renilla luciferase constructs (Promega #E2231) driven by a TK promoter were co-transfected with promoter constructs at a 1:5 ratio. To test the effect

(175) Characterization of SARS-CoV-2 nucleocapsid protein reveals multiple functional consequences of the C-terminal domainiScienceJune 25, 2021Chao Wu et al.Medicine at Mount Sinai, New York, NY USA",N/A pRL-TK Renilla luciferase reporter plasmid,Promega,E2231 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Intact Mass™,Protein

(176) The Inducible lac Operator-Repressor System Is Functional in Zebrafish CellsFrontiers in GeneticsJune 18, 2021Sierra S. Nishizaki, Torrin L. McDonald, Gregory A. Farnum, Monica J. Holmes, Melissa L. Drexel, Jessica A. Switzenberg, Alan P. Boyleelectroporation (Figure 1). 100 ng of the renilla luciferase expressing plasmid (pRL-SV40 Promega, E2231) was included as a transfection control. Firefly/renilla luciferase signal was calculated as the mean

(177) Role of nuclear localization in the regulation and function of T-bet and Eomes in exhausted CD8 T cellsCell reportsMay 11, 2021McLane LM, Ngiow SF, Chen Z, Attanasio J, Manne S, Ruthel G, Wu JE, Staupe RP, Xu W, Amaravadi RK, et al.vector,Promega, pGL3-Pdcd1 CR B+C,"Oestreich et al., 2008", pRL Renilla luciferase control vector,Promega,Cat# E2231 MSCV-T-bet-ER vector,This study,N/A MSCV-GFP vector,This study,N/A Software and algorithms,Software

(178) FYN–TRAF3IP2 induces NF-κB signaling-driven peripheral T-cell lymphomaNature CancerApril 30, 2021Christine S. Moon et al.reporter plasmid (Promega #E8491) and 50 ng constitutively expressed Renilla luciferase plasmid (Promega #E2231) together with 1 ng of empty pcDNA3.1 vector or pcDNA3.1 constructs encoding wild type TRAF3IP2, fusion

(179) Post-transcriptional repression of circadian component CLOCK regulates cancer-stemness in murine breast cancer cellseLifeApril 23, 2021Ogino T, Matsunaga N, Tanaka T, Tanihara T, Terajima H, Yoshitane H, Fukada Y, Tsuruta A, Koyanagi S, Ohdo S.reagent pGL4.13 (plasmid) Promega Cat #: E6681 Recombinant DNA reagent pRL-SV40 (plasmid) Promega Cat #: E2231 Sequencebased reagent Primer for sgRNA synthesis This paper Sequencebased reagent Mouse Clock promoter

(180) Regulation of PTEN translation by PI3K signaling maintains pathway homeostasisMolecular cellFebruary 18, 2021Mukherjee R, Vanaja KG, Boyer JA, Gadal S, Solomon H, Chandarlapaty S, Levchenko A, Rosen N.Accession Number U47298) Cat No #E1751 pRL-CMV Vector,Promega,"(GenBank Accession Number AF025843), Cat No-E2231" pRL-CMV-IRES Vector,This paper,NA pCW57.1–4EBP1_4xAla,Addgene,Cat# 38240 LentiCRISPRv2,Addgene,Cat#52961 TTIGFP-MLUEX,Lab

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

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Reviews

pRL-SV40 Vector from Promega

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