pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failurePLoS pathogensAugust 1, 2024Li H, Wan L, Liu M, Ma E, Huang L, Yang Y, Li Q, Fang Y, Li J, Han B, et al.response element was obtained from Beyotime (D2204). The pSV40-Renilla plasmid was purchased from Promega (E2231). To assess the activity of NF-κB, cells were plated in triplicate in a 24-well plate and then transiently

(82) Evidence for NR2F2/COUP-TFII involvement in human testis developmentScientific ReportsAugust 1, 2024Somboon Wankanit, Housna Zidoune, Joëlle Bignon-Topalovic, Laurène Schlick, Denis Houzelstein, Leila Fusée, Asma Boukri, Nassim Nouri, Ken McElreavey, Anu Bashamboo, et al.Dual Luciferase Reporter Assay system (#E1910, Promega) with pCMV-RL Renilla luciferase (pRL Renilla (#E2231, Promega)) expression as a marker of transfection efficiency. For each well of 96-well, 25,000 HEK293-T cells

(83) Downregulation of 4-HNE and FOXO4 collaboratively promotes NSCLC cell migration and tumor growthCell Death & DiseaseJuly 31, 2024Tianfei Zhong, Ying Li, Meng Jin, Jingqun Liu, Zhenyu Wu, Feiye Zhu, Lisha Zhao, Yongsheng Fan, Li Xu, Jinjun JiAddgene), Foxo NLuc luciferase reporter (178318, Addgene), pRL Renilla Luciferase Control Reporter Vector (E2231, Promega), Dual-Luciferase® reporter assay system (E1910, Promega). PrimeScript™ RT Master Mix (Perfect Real Time

(84) miR-340-3p-modified bone marrow mesenchymal stem cell-derived exosomes inhibit ferroptosis through METTL3-mediated m(6)A modification of HMOX1 to promote recovery of injured rat uterusStem cell research & therapyJuly 29, 2024Xiao B, Zhu Y, Liu M, Chen M, Huang C, Xu D, Wang F, Sun S, Huang J, Sun N, et al.transfection efficiency was monitored by co-transfection of the internal monitor phRL-null (Promega, E2231) with the luciferase reporter vectors. Quantification of m6A RNA methylation The EpiQuik™ kit (Colorimetric)

(85) ONECUT2 acts as a lineage plasticity driver in adenocarcinoma as well as neuroendocrine variants of prostate cancerNucleic Acids ResearchJuly 22, 2024Chen Qian, Qian Yang, Mirja Rotinen, Rongrong Huang, Hyoyoung Kim, Brad Gallent, Yiwu Yan, Radu M Cadaneanu, Baohui Zhang, Salma Kaochar, et al.Luciferase reporter assay Cells were co-transfected with PGL4.10 luciferase backbone construct, and the pRL-SV40 vector (Promega #E2231) with Turbofectin 8.0 (Origene, #TF81001). After transfection overnight, cells were washed and treated

(86) Application of prime editing system to introduce TP53 R248Q hotspot mutation in acute lymphoblastic leukemia cell lineCancer ScienceJune 1, 2024Thao Nguyen, Tomomi Aida, Yuka Iijima‐Yamashita, Minori Tamai, Akiko Nagamachi, Keiko Kagami, Chiaki Komatsu, Shin Kasai, Koshi Akahane, Kumiko Goi, et al.vectors using Lipofectamine 3000 (Thermo Fisher Scientific). Renilla luciferase reporter plasmid pTK‐RL (E2231; Promega) was cotransfected as the internal standard. Twenty‐four hours after transfection, the cells

(87) Temporal dynamics and stoichiometry in human Notch signaling from Notch synaptic complex formation to nuclear entry of the Notch intracellular domainDevelopmental CellJune 1, 2024Lena Tveriakhina, Gustavo Scanavachi, Emily D. Egan, Ricardo Bango Da Cunha Correia, Alexandre P. Martin, Julia M. Rogers, Jeremy S. Yodh, Jon C. Aster, Tom Kirchhausen, Stephen C. BlacklowZhang",RRID:Addgene_48139 pUC19,Addgene,RRID:Addgene_50005 mEos4b-N1,Addgene,RRID:Addgene_54814 pGa981-6; TP1-luc,"Kurooka et al., 199863; Minoguchi et al., 199764", pRL-TK,Promega,Cat# E2231 pFUSE-hIgG1-Fc,Invivogen,Cat code: pfuse-hg1fc1 pFUSE-Jagged1ECD-hIgG1-Fc1,"Martin et al., 202332,

(88) NANOG controls testicular germ cell tumour stemness through regulation of MIR9-2Stem cell research & therapyMay 1, 2024Cardenas RP, Zyoud A, McIntyre A, Alberio R, Mongan NP, Allegrucci C.expressing the 464 bp genomic sequence fragment upstream of MIR9-2 and the Renilla plasmid (Promega E2231) were co-transfected into cells and luciferase activity was measured after 2 days. The NANOG binding

(89) Islet-resident macrophage-derived miR-155 promotes β cell decompensation via targeting PDX1iScienceApril 19, 2024Yan Zhang, Rong Cong, Tingting Lv, Kerong Liu, Xiaoai Chang, Yating Li, Xiao Han, Yunxia Zhupaper,N/A PDX1-mt,This paper,N/A pCMV5-Flag,This paper,N/A pCMV5-Flag -PDX1,This paper,N/A pRL-SV40,Promega,Cat#E2231 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, FV10-ASW,Olympus,https://lifescience.evidentscientific.com.cn/zh/downloads/detail-iframe/?0[downloads][id]=847249651 ImageJ,NIH,https://imagej.nih.gov/ij/ Pannoramic

(90) Keratinocyte integrin α3β1 induces expression of the macrophage stimulating factor, CSF-1, through a YAP/TEAD-dependent mechanismMatrix BiologyMarch 1, 2024Whitney M. Longmate, Emily Norton, Giesse Albeche Duarte, Lei Wu, Mathieu R. DiPersio, John M. Lamar, C. Michael DiPersioof the Firefly Luciferase gene [43]. A control Renilla luciferase construct (PRL-TK (Promega, Cat. #E2231)) was co-transfected and used for normalization. Cells plated on 12-well dishes in duplicate were co-transfected

(91) No link between type I interferon autoantibody positivity and adverse reactions to COVID-19 vaccinesnpj VaccinesFebruary 22, 2024Ahmet Yalcinkaya, Marco Cavalli, Axel Cederholm, Maribel Aranda-Guillén, Anish Behere, Hedvig Mildner, Tadepally Lakshmikanth, Laura Gonzalez, Constantin Habimana Mugabo, Anette Johnsson, et al.pGL4.45[luc2P/ISRE/Hygro] and Renilla pRL-SV40 internal control luciferase vectors (Promega; #E414A and #E2231, respectively). The transfection solution was created in OptiMEM media with a 3:1 (ul:ug) ratio between

(92) Identification of a Gene Signature That Predicts Dependence upon YAP/TAZ-TEADCancers (Basel)February 20, 2024Ryan Kanai, Emily Norton, Patrick Stern, Richard O. Hynes, John M. Lamarvector encoding a constitutively expressed Renilla luciferase (PRL-TK (Promega, Madison, WI, USA, Cat#E2231)) is co-transfected and used for normalization. Cells were plated at 1.5 × 105 cells per 12-well in

(93) VAV2 orchestrates the interplay between regenerative proliferation and ribogenesis in both keratinocytes and oral squamous cell carcinomaScientific reportsFebruary 19, 2024Fernández-Parejo N, Lorenzo-Martín LF, García-Pedrero JM, Rodrigo JP, Dosil M, Bustelo XR.of Sciences, 200031 Shanghai, China) and pRL-SV40 (Renilla luciferase, obtained from Promega, cat. #E2231). The DNA sequences of all plasmids were verified. Cells Primary human keratinocytes (Ker-CT cell

(94) Multi-targeted loss of the antigen presentation molecule MR1 during HSV-1 and HSV-2 infectioniScienceFebruary 16, 2024Samer C, McWilliam HEG, McSharry BP, Velusamy T, Burchfield JG, Stanton RJ, Tscharke DC, Rossjohn J, Villadangos JA, Abendroth A, et al.,BD Biosciences,Cat#554655 Folimycin (concanamycin A),Sigma-Aldrich,Cat#C9705 MG132,Sigma-Aldrich,Cat#M7449 Fugene HD,Promega,Cat#E2231 Q5 High-Fidelity polymerase,NEB,Cat#M0491 Endo H,NEB,Cat#P0703S DAB Substrate,Pierce,Cat#34065 BamHI-HF,NEB,Cat#R3136 SpeI-HF,NEB,Cat#R3133 BglII,NEB,Cat#R0144 XbaI,NEB,Cat#R0145 Xgal,Abcam,Cat#ab144388 IPTG,Abcam,Cat#ab142072 HindIII,NEB,Cat#R0104 T4 DNA ligase

(95) Gut-liver axis calibrates intestinal stem cell fitnessCellFebruary 15, 2024Girak Kim, Zuojia Chen, Jian Li, Jialie Luo, Felipe Angeles Castro Castro-Martinez, Jan Wisniewski, Kairong Cui, Yan Wang, Jialei Sun, Xiaobai Ren, et al.CTGGTGCTACTCCTCTGGAC,IDT,N/A Recombinant DNA,Recombinant DNA,Recombinant DNA pGL3 Basic,Promega,Cat# E1751 pRL Renilla,Promega,Cat# E2231 px459,Addgene,Cat# 62988 pAAV-sgNTC,This paper,N/A pAAV-sgPedf,This paper,N/A pSG5 PPAR alpha,Addgene,Cat#

(96) Multi-Omics Profiling Identifies Microglial Annexin A2 as a Key Mediator of NF-κB Pro-inflammatory Signaling in Ischemic Reperfusion InjuryMolecular & cellular proteomics : MCPFebruary 1, 2024Tian X, Yang W, Jiang W, Zhang Z, Liu J, Tu H.and cotransfected with pNF-κB-Luc (Addgene, 49343) and Renilla luciferase reporter plasmids (Promega, E2231) using Lipofectamine 2000 for 24 h followed by OGD/R. The luciferase activities were determined using

(97) Integrative functional genomic analyses identify genetic variants influencing skin pigmentation in AfricansNature GeneticsFebruary 1, 2024Yuanqing Feng, Ning Xie, Fumitaka Inoue, Shaohua Fan, Joshua Saskin, Chao Zhang, Fang Zhang, Matthew E. B. Hansen, Thomas Nyambo, Sununguko Wata Mpoloka, et al.0.1M per well, and 500 ng firefly luciferase plasmid, 20 ng pRL Renilla luciferase plasmid (Promega, # E2231) and 1.5 μL Lipofectamine 3000 (Invitrogen, #L3000150) were added to each well. 36 hours post-transfection,

(98) 3'-Sialyllactose alleviates bone loss by regulating bone homeostasisCommunications biologyJanuary 19, 2024Baek A, Baek D, Cho Y, Jo S, Kim J, Hong Y, Cho S, Kim SH, Cho SR.or NFATc1-binding promoter element plasmid (#E8481, Promega) along with Renilla luciferase vector (#E2231, Promega) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions. After

(99) DDX5 inhibits inflammation by modulating m6A levels of TLR2/4 transcripts during bacterial infectionEMBO ReportsJanuary 5, 2024Jian Xu, Li-Yuan Liu, Fei-Jie Zhi, Yin-Juan Song, Zi-Hui Zhang, Bin Li, Fu-Ying Zheng, Peng-Cheng Gao, Su-Zi Zhang, Yu-Yu Zhang, et al.DNA pEGF-N1,TIANDZ,60908-2680 p3×FLAG-CMV-10,zomanbio,ZK26130 pCMV-Myc,TIANDZ,60908-2050 pCMV-HA,TIANDZ,60908-2020 IL-6 luciferase reporter,"Jinhai Huang (Tianjin University, China)",N/A pRL-TK Vector,Promega,E2231 Antibodies,Antibodies,Antibodies Mouse anti-METTL14 monoclonal antibody,Abcam,ab220030 Rabbit anti-TLR2

(100) ELK3-ID4 axis governs the metastatic features of triple negative breast cancerOncology ResearchJanuary 1, 2024JIN-HO CHOI, JOO DONG PARK, SEUNG HEE CHOI, EUN-SU KO, HYE JUNG JANG, KYUNG-SOON PARKNo.,Name,Information 1,pLenti-cMyc-DDK,"Origene, PS1000064" 2,pLenti-ELK3-cMyc-DDK,NM_005230.3 3,pRL-TK,"Promega, E2231" 4,pGL3-basic,"Promega, E1751" 5,pGL3-hID4 promoter (−1098~+100 bp),– 6,pGL3-mut hID4 promoter,– 7,Nonspecific

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

Citations (323)

Reviews

pRL-SV40 Vector from Promega

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