pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(141) A promoter variant in the OTC gene associated with late and variable age of onset hyperammonemiaJournal of Inherited Metabolic DiseaseJuly 1, 2022Sangwoo T. Han, Katherine J. Anderson, Hans T. Bjornsson, Nicola Longo, David Vallereporter constructs containing the cloned promoter fragments and enhancer sequences, and Renilla plasmids (E2231 Promega, Milwaukee WI) in triplicate using Lipofectamine 3000 (L3000015 Invitrogen, Carlsbad CA). Lysates

(142) Pulmonary Alveolar Proteinosis and Multiple Infectious Diseases in a Child with Autosomal Recessive Complete IRF8 DeficiencyJournal of clinical immunologyJuly 1, 2022Rosain J, Bernasconi A, Prieto E, Caputi L, Le Voyer T, Buda G, Marti M, Bohlen J, Neehus AL, Castaños C, et al.reporter plasmid (100 ng/well and 100 µL of DMEM-10% FBS medium) and the pRL-SV40 plasmid (Promega, #E2231, 40 ng/well), with or without the IRF1 WT p.CMV6 plasmid (50 ng/well), with or without IRF8-WT or mutant

(143) A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signalingCellJune 23, 2022Alphonse N, Wanford JJ, Voak AA, Gay J, Venkhaya S, Burroughs O, Mathew S, Lee T, Evans SL, Zhao W, et al.study,N/A pEGFP-C1-OspC1-EH/AA,This study,N/A pEGFP-C1-OspC3- EH/AA,This study,N/A pRL-CMV-Renilla,Promega,Cat#E2231 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Fiji,Fiji v 2.1.0,RRID:

(144) cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS modeliScienceJune 17, 2022Tan HY, Yong YK, Xue YC, Liu H, Furihata T, Shankar EM, Ng CS.(luc2P/NF-κB-RE/Hygro),Promega,Cat#E8491 pRL-TK Renilla Luciferase Control reporter vector,Promega,Cat#E2231 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Prism version 8.0,GraphPad,https://www.graphpad.com/ Microsoft

(145) Iloprost requires the Frizzled-9 receptor to prevent lung canceriScienceJune 17, 2022Sompel K, Dwyer-Nield LD, Smith AJ, Elango A, Backos DS, Zhang B, Gross J, Ternyak K, Matsuda JL, Kopf K, et al.DNA,Recombinant DNA,Recombinant DNA ,, PPRE,Addgene,"RRID: Addgene_1015,#1050" Renilla,Promega,#E2231 Y277S_FZD9 C-term his tag,Genescript,Lot: U279VGC020-1/Q87680 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, Ces1

(146) miR‐181b regulates vascular endothelial aging by modulating an MAP3K3 signaling pathwayThe FASEB JournalJune 1, 2022Haoyang Zhou, Dafeng Yang, Henry S. Cheng, Michael G. McCoy, Daniel Pérez‐Cremades, Stefan Haemmig, Danny Wong, Lei Chen, Mark W. Feinbergtransfected with 100 ng of wild-type or mutant MEKK3 reporter constructs and 10 ng Renilla plasmid (E2231, Promega). NS-m or 181b-m was co-transfected at 10 nM final concentration for 12 hours. Transfected

(147) miR-181b regulates vascular endothelial aging by modulating a MAP3K3 signaling pathwayFASEB JJune 1, 2022Haoyang Zhou, Dafeng Yang, Henry S. Cheng, Michael G McCoy, Daniel Pérez-Cremades, Stefan Haemmig, Danny Wong, Lei Chen, Mark W. Feinbergtransfected with 100 ng of wild-type or mutant MEKK3 reporter constructs and 10 ng Renilla plasmid (E2231, Promega). NS-m or 181b-m was co-transfected at 10 nM final concentration for 12 hours. Transfected

(148) The COX-2-PGE2 Pathway Promotes Tumor Evasion in Colorectal AdenomasCancer prevention research (Philadelphia, Pa.)May 3, 2022Wei J, Zhang J, Wang D, Cen B, Lang JD, DuBois RN.THP-1 cells were transfected with 5 μg of a mouse or human PD-1-Luc plasmid and 0.2 μg pRL-TK (Promega, Cat#E2231) DNA as a control using a Cell line Nucleofector Kit V (Lonza, Cat#VCA-1003) according to the manufacturer’s

(149) Identification of ATP2B4 Regulatory Element Containing Functional Genetic Variants Associated with Severe MalariaInternational Journal of Molecular SciencesApril 27, 2022Samia Nisar et al.CTCCG, CTTGG, or CTTCA) and 200 ng of pRL-SV40 (a plasmid encoding renilla luciferase from Promega (cat# E2231)), which was used as a transfection efficiency control. Transfected cells were maintained at 37 °C in

(150) Calcineurin/NFATc2 and PI3K/AKT signaling maintains β-cell identity and function during metabolic and inflammatory stressiScienceApril 15, 2022Darden CM, Vasu S, Mattke J, Liu Y, Rhodes CJ, Naziruddin B, Lawrence MC.pGL3-NFAT-Luc,"Clipstone and Crabtree, 1992",Addgene plasmid #17870; RRID:Addgene_17870 Plasmid: pRL-SV40,Promega,Cat#E2231 Slc16a1 Promoter Reporter Clone,Genecopoeia,MPRM40340-PG04 Rfx6 Promoter Reporter Clone,Genecopoeia,MPRM33989-PG04 ,, Software

(151) Ivermectin represses Wnt/β-catenin signaling by binding to TELO2, a regulator of phosphatidylinositol 3-kinase-related kinasesiScienceMarch 18, 2022Yonezawa H, Ikeda A, Takahashi R, Endo H, Sugawara Y, Goto M, Kanno M, Ogawa S, Nakamura K, Ujiie H, et al.DNA,Recombinant DNA ,, Super 8x TOPFlash,Addgene,"Cat# 12456 (Veeman et al., 2003)" pRL-SV40,Promega,Cat# E2231 p3xFLAG-CMV10-hTel2,Addgene,"Cat# 30214 (Kaizuka et al., 2010)" p3xFLAG-CMV10-hTel2 K749T,This article,N/A pGEX6p1-TELO2

(152) Transcription factor protein interactomes reveal genetic determinants in heart diseaseCellMarch 3, 2022Gonzalez-Teran B, Pittman M, Felix F, Thomas R, Richmond-Buccola D, Hüttenhain R, Choudhary K, Moroni E, Costa MW, Huang Y, et al.luciferase reporter vector and 20 ng of Renilla luciferase control vector pRL-TK or SV40 (Cat. E2241 or E2231, Promega) in 2.4 μl FuGENE HD (Cat. E2311, Promega) and 43 μl Opti-MEM (Cat. 31985070, Thermo Fisher

(153) An inducible CRISPR/Cas9 screen identifies DTX2 as a transcriptional regulator of human telomeraseiScienceFebruary 18, 2022Zhifen Zhou et al.firefly luciferase expression. An SV40 promoter-driven Renilla luciferase vector (pRL-SV40, Promega, E2231) served as an internal control. Expression plasmids and pRL-SV40 were co-transfected with pGL4.10-HES1P

(154) Epigenetic Dysregulation of the Homeobox A5 (HOXA5) Gene Associates with Subcutaneous Adipocyte Hypertrophy in Human ObesityCellsFebruary 18, 2022Parrillo L, Spinelli R, Costanzo M, Florese P, Cabaro S, Desiderio A, Prevenzano I, Raciti GA, Smith U, Miele C, et al.of each transfection was normalized against renilla luciferase activity (Promega, Madison, WI, USA, #E2231). 2.1.10. Peripheral Blood Leukocyte Isolation Peripheral blood leukocytes (PBLs) were isolated as

(155) Uncovering the mesendoderm gene regulatory network through multi-omic data integrationCell ReportsFebruary 15, 2022Camden Jansen et al.DNA,Recombinant DNA ,, – 104 gsc minimal promoter-pOLuc,Watabe et al. (1995),N/A pRL-SV40,Promega,Cat#E2231 zic2 Luc reporter,This Paper,N/A zic2 mutant Luc reporter,This Paper,N/A foxa2 Luc reporter,This

(156) Disruption of pancreatic stellate cell myofibroblast phenotype promotes pancreatic tumor invasionCell reportsJanuary 25, 2022Murray ER, Menezes S, Henry JC, Williams JL, Alba-Castellón L, Baskaran P, Quétier I, Desai A, Marshall JJT, Rosewell I, et al.(pGL3-4xGTIIC-49),"Dr. Nic Tapon; Mahoney et al., 2005",N/A Renilla plasmid (pRL),Promega,Cat. No.: E2231 pBabeSV40 Large T,"Prof. Parmjit Jat; Cotsiki et al., 2004",N/A ,, "Chemicals, peptides, and recombinant

(157) Three of a Kind: Control of the Expression of Liver-Expressed Antimicrobial Peptide 2 (LEAP2) by the Endocannabinoidome and the Gut MicrobiomeMolecules (Basel, Switzerland)December 21, 2021Shen M, Manca C, Suriano F, Nallabelli N, Pechereau F, Allam-Ndoul B, Iannotti FA, Flamand N, Veilleux A, Cani PD, et al.pM1-hPPARγ-Gal4; (b) TK-MH100 × 4-Luc containing the UAS enhancer elements and; (c) Renilla luciferase (pRL, Cat. E2231; Promega, Milan, Italy) using lipofectamine 2000 (cat. n. 11668027; Life Technologies; Milan, Italy).

(158) FoxO-KLF15 pathway switches the flow of macronutrients under the control of insuliniScienceDecember 17, 2021Takeuchi Y, Yahagi N, Aita Y, Mehrazad-Saber Z, Ho MH, Huyan Y, Murayama Y, Shikama A, Masuda Y, Izumida Y, et al.2017)",N/A pENTR4 plasmid,Thermofisher,11818-010 pENTR/U6 plasmid,Thermofisher,K494500 pRL-SV40,Promega,E2231 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, ImageJ 1.50i,NIH,https://imagej.nih.gov/ij/ JASPAR2020,JASPAR,https://jaspar.genereg.net/ Living

(159) Domain-specific biochemical and serological characterization of SARS-CoV-2 nucleocapsid proteinSTAR ProtocolsDecember 17, 2021Chao Wu et al.Medicine at Mount Sinai, New York, NY USA",N/A pRL-TK Renilla luciferase reporter plasmid,Promega,E2231 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Intact Mass™,Protein

(160) NUDT2 initiates viral RNA degradation by removal of 5'-phosphatesNature communicationsNovember 25, 2021Laudenbach BT, Krey K, Emslander Q, Andersen LL, Reim A, Scaturro P, Mundigl S, Dächert C, Manske K, Moser M, et al.oligodeoxynucleotides (Supplementary Table 3). Except, the template for the generation of renilla RNA (Promega, E2231), which PCR amplified according to the manufacturer’s instructions (2x HF Mastermix, NEB M0541S) with

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

Citations (323)

Reviews

pRL-SV40 Vector from Promega

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