pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(181) N-palmitoyl-D-glucosamine, a Natural Monosaccharide-Based Glycolipid, Inhibits TLR4 and Prevents LPS-Induced Inflammation and Neuropathic Pain in MiceInternational journal of molecular sciencesFebruary 2, 2021Iannotta M, Belardo C, Trotta MC, Iannotti FA, Vitale RM, Maisto R, Boccella S, Infantino R, Ricciardi F, Mirto BF, et al.IT; cat. no. E8491) and (b) a plasmid encoding for Renilla control vector (Promega, IT; cat. no. Cat. E2231) was used to normalize the signals and as positive control vector for monitoring transfection efficiency.

(182) Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a regulator of insulin secretionCell ReportsFebruary 2, 2021Ming Hu et al.DNA,bacpacresources.org,RP11-101P7 pLenti-CRISPR-RIP-Cas9,Paul Gadue,N/A pGL3-promoter,Promega,E1761 pRL-Renilla,Promega,E2231 pBlueScript II KS+,Stratagene/Agilent,212207 ,, Software,Software,Software ,, GraphPad Prism 7,GraphPad

(183) Beneficial Effects of Akkermansia muciniphila Are Not Associated with Major Changes in the Circulating Endocannabinoidome but Linked to Higher Mono-Palmitoyl-Glycerol Levels as New PPARα AgonistsCellsJanuary 19, 2021Depommier C, Vitale RM, Iannotti FA, Silvestri C, Flamand N, Druart C, Everard A, Pelicaen R, Maiter D, Thissen JP, et al.pM1-hPPARγ-Gal4; (b) TK-MH100 × 4-Luc containing the UAS enhancer elements and; (c) Renilla luciferase (pRL, Cat. E2231; Promega, Milan, Italy) using lipofectamine 2000 (cat. n. 11668027; Life Technologies; Milan, Italy)

(184) The mTOR regulated RNA-binding protein LARP1 requires PABPC1 for guided mRNA interactionNucleic acids researchJanuary 11, 2021Smith EM, Benbahouche NEH, Morris K, Wilczynska A, Gillen S, Schmidt T, Meijer HA, Jukes-Jones R, Cain K, Jones C, et al.well of RPS16-PEST-CL reporter and 100 ng per well of control pRL-SV40 vector (Promega catalogue # E2231) used as control vector were added and transfection mix incubated for 30 min at RT. Then 20 μl of transfection

(185) Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathwayeLifeJanuary 8, 2021Yang D, Haemmig S, Zhou H, Pérez-Cremades D, Sun X, Chen L, Li J, Haneo-Mejia J, Yang T, Hollan I, et al.indicated Mir181a2b2 promoter reporter constructs (Supplementary file 1) and 10 ng Renilla plasmid (E2231, Promega). Adenosine receptor siRNAs and SMAD2/3/4 siRNAs were co-transfected at 100 or 20 nM final

(186) Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosisCell & bioscienceJanuary 6, 2021Hwang I, Lee EJ, Park H, Moon D, Kim HS.the pGL3-RARE construct (Cat. 13458; Addgene, Waterown, MA, USA) and the pRL-renilla construct (Cat. E2231; Promega, Madison, WI, USA) as a positive control for transfection efficiency. The Dual-Luciferase Reporter Assay System

(187) The forkhead box transcription factor FoxP4 regulates thermogenic programs in adipocytesJournal of lipid researchJanuary 1, 2021Perie L, Verma N, Mueller E.the pGL3 luciferase reporter vector (#E1751) and the pRL Renilla luciferase control reporter vector (#E2231) were purchased from Promega. The UCP1 (2.1 kb) luciferase plasmid was a kind gift of Dr Evan Rosen

(188) Dihydromyricetin Inhibits Tumor Growth and Epithelial-Mesenchymal Transition through regulating miR-455-3p in CholangiocarcinomaJournal of CancerJanuary 1, 2021Li X, Yang ZS, Cai WW, Deng Y, Chen L, Tan SL.plates and co-transfected with 250 ng WT or Mut luciferase reporter constructs, 10 ng Renilla plasmid (E2231, Promega, USA), 100 nM 455-m or NS-m, together with lipofectamine 2000 after cells growing to 70% confluency.

(189) Design and Evaluation of Guide RNA Transcripts with a 3'-Terminal HDV Ribozyme to Enhance CRISPR-Based Gene InactivationMethods in molecular biology (Clifton, N.J.)January 1, 2021Berkhout B, Gao Z, Herrera-Carrillo E.#7209).Control Firefly Luciferase Reporter (pGL3) (Promega #E1741).Renilla Luciferase Control (pRL) (Promega #E2231).pCMV-rtTA-V10 plasmid [17].pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene# 12252).One Shot Stbl3 chemically competent

(190) MicroRNA-455-5p Contributes to Cholangiocarcinoma Growth and Mediates Galangin's Anti-Tumor EffectsJournal of CancerJanuary 1, 2021Deng X, Zuo M, Pei Z, Xie Y, Yang Z, Zhang Z, Jiang M, Kuang D.confluency, co-transfected with 250 ng WT or Mut luciferase reporter constructs, 10 ng Renilla plasmid (E2231, Promega, USA) and 100 nM 455-m or NS-m using lipofectamine 2000 according to the manufacturer's protocols.

(191) The p53-induced RNA-binding protein ZMAT3 is a splicing regulator that inhibits the splicing of oncogenic CD44 variants in colorectal carcinomaGenes & developmentJanuary 1, 2021Muys BR, Anastasakis DG, Claypool D, Pongor L, Li XL, Grammatikakis I, Liu M, Wang X, Prasanth KV, Aladjem MI, et al.RE element, respectively, found in intron 1 from ZMAT3, were cloned into pGL3-Basic vector (Promega E2231) for luciferase assays. We used gBlocks gene fragments (IDT) at region chr3:179,069,907–179,070,507

(192) A Functional SMAD2/3 Binding Site in the PEX11β Promoter Identifies a Role for TGFβ in Peroxisome Proliferation in HumansFrontiers in Cell and Developmental BiologyOctober 23, 2020Afsoon S. Azadi, Ruth E. Carmichael, Werner J. Kovacs, Janet Koster, Suzan Kors, Hans R. Waterham, Michael SchraderPromega). The pGL3-basic vector without a promoter was used as a negative control. pRL-TK vector (#E2231, Frontiers in Cell and Developmental Biology | www.frontiersin.org 1 3 http://crispr.mit.edu/ October

(193) Gm614 Protects Germinal Center B Cells From Death by Suppressing Caspase-1 Transcription in Lupus-Prone MiceFrontiers in ImmunologyOctober 21, 2020Youdi He, Ruonan Xu, Bing Zhai, Shan Zhou, Xiaoqian Wang, Renxi Wangempty LV 201 (Vector) plasmid, and 0.05 µg Renilla luciferase reporter vector pRL-SV-40 vector (cat# E2231, Promega Corp.) were co-transduced into 4 X 105 293T cells in 12-well plate by using 6 µL Lipofectamine®2000

(194) Neural Stem Cells Direct Axon Guidance via Their Radial Fiber ScaffoldNeuronSeptember 23, 2020Kaur N, Han W, Li Z, Madrigal MP, Shim S, Pochareddy S, Gulden FO, Li M, Xu X, Xing X, et al.mCherry,Addgene,Cat#63721 pmScarlet-i_C1,Addgene,Cat#85044 pGL3 basic,Promega,Cat#E1751 pRL-SV40,Promega,Cat#E2231 pCAG-Bfp,"Rakic Lab, Yale University",N/A pCAG-V5-Fezf2,"Sestan Lab, Yale University",N/A pNeuroD1-Cre,"Sestan

(195) Chromatin accessibility mapping of the striatum identifies tyrosine kinase FYN as a therapeutic target for heroin use disorderNature communicationsSeptember 14, 2020Egervari G, Akpoyibo D, Rahman T, Fullard JF, Callens JE, Landry JA, Ly A, Zhou X, Warren N, Hauberg ME, et al.day of transfection, cells were co-transfected with pGL3 control vector and pRL-SV40 vector (Promega, E2231) or hFyn-pGL3 vector and pRL-SV40 vector at a 10:1 ratio of pRL-SV40 vector to pGL3 vector in 200 ng

(196) Hypoxia-induced TGF-β-RBFOX2-ESRP1 axis regulates human MENA alternative splicing and promotes EMT in breast cancerNAR cancerSeptember 1, 2020Ahuja N, Ashok C, Natua S, Pant D, Cherian A, Pandkar MR, Yadav P, Vishnu NSS, Mishra J, Samaiya A, et al.with different ESRP1 promoter–luciferase constructs, and pRL-TK Renilla luciferase plasmid (Promega, E2231) harvested at 48 h and lysed in passive lysis buffer. For TGF-β and normoxic/hypoxic experiments, cells

(197) Identification of an Anti-diabetic, Orally Available Small Molecule that Regulates TXNIP Expression and Glucagon ActionCell metabolismSeptember 1, 2020Thielen LA, Chen J, Jing G, Moukha-Chafiq O, Xu G, Jo S, Grayson TB, Lu B, Li P, Augelli-Szafran CE, et al.deletions),Minn et al. 2005,N/A SV40 E-box plasmid,Minn et al. 2005,N/A pRL-TK control plasmid,Promega,E2231 ,, Software and Algorithms,Software and Algorithms,Software and Algorithms StarDrop,Optibrium,https://www.optibrium.com/ ImageQuant

(198) LncRNA MALAT1 promoted high glucose-induced pyroptosis of renal tubular epithelial cell by sponging miR-30c targeting for NLRP3The Kaohsiung journal of medical sciencesSeptember 1, 2020Liu C, Zhuo H, Ye MY, Huang GX, Fan M, Huang XZ.and corresponding mutant‐type fragments (MUT) were amplified and inserted into psiCHECK‐2 plasmid (E2231; Promega, Madison, Wisconsin). Similarly, the WT fragment from the NLRP3 3′‐untranslated region (UTR)

(199) Vav2 pharmaco-mimetic mice reveal the therapeutic value and caveats of the catalytic inactivation of a Rho exchange factorOncogeneJuly 9, 2020L. Francisco Lorenzo-Martín, Sonia Rodríguez-Fdez, Salvatore Fabbiano, Antonio Abad, María C. García-Macías, Mercedes Dosil, Myriam Cuadrado, Javier Robles-Valero, Xosé R. Busteloactivity; Agilent, Catalog No. 219081), and pRL-SV40 (encoding the Renilla luciferase; Promega, Catalog No. E2231). Rho GTPase activation assays in COS1 cells Exponentially growing COS1 cells were transfected using

(200) Integrated Structural Modeling of Full-Length LRH-1 Reveals Inter-domain Interactions Contribute to Receptor Structure and FunctionStructure (London, England : 1993)July 7, 2020Seacrist CD, Kuenze G, Hoffmann RM, Moeller BE, Burke JE, Meiler J, Blind RD.Fearon pcDNA3 human DAX-1,Genscript,OHu28295 CYP17A1-pGL3-Basic,,A gift from Holly Ingraham pRL-SV40,Promega,E2231 pRL-TK,Promega,E2241 Oligonucleotides,Oligonucleotides,Oligonucleotides CYP7A1 Forward Promoter:

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

Citations (323)

Reviews

pRL-SV40 Vector from Promega

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