pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) Serine-arginine protein kinase 1 (SRPK1) promotes EGFR-TKI resistance by enhancing GSK3β Ser9 autophosphorylation independent of its kinase activity in non-small-cell lung cancerOncogeneApril 6, 2023Jing-Qiang Huang, Ling-Xin Duan, Qiu-Yu Liu, He-Feng Li, Ao-Ping Hu, Jun-Wei Song, Chuxuan Lin, Bingsheng Huang, Da Yao, Bin Peng, et al.plates and then transiently transfected with TOP FLASH or FOP FLASH and Renilla pRL-TK plasmids. (cat: E2231; Promega) using Lipofectamine 3000 Reagent (cat: 11668019, Thermo Fisher Scientific). After 48 h, relative

(122) Whole-genome sequencing reveals a complex African population demographic history and signatures of local adaptationCellMarch 2, 2023Shaohua Fan, Jeffrey P. Spence, Yuanqing Feng, Matthew E. B. Hansen, Jonathan Terhorst, Marcia H. Beltrame, Alessia Ranciaro, Jibril Hirbo, William Beggs, Neil Thomas, et al.0.1M per well, and 500 ng firefly luciferase plasmid, 20 ng pRL Renilla luciferase plasmid (Promega, # E2231) and 1.5 µL Lipofectamine 3000 (Invitrogen, #L3000150) were added to each well. 36 hours post transfection,

(123) TGFβ-mediated MMP13 secretion drives myoepithelial cell dependent breast cancer progressionnpj Breast CancerMarch 2, 2023Shayin V. Gibson, Elena Tomas Bort, Lucía Rodríguez-Fernández, Michael D. Allen, Jennifer J. Gomm, Iain Goulding, Ulrich auf dem Keller, Andrea Agnoletto, Cathrin Brisken, Barrie Peck, et al.cells per well of a 24-well plate were co-transfected with 170 ng Renilla luciferase control plasmid (E2231/E2261, pRL, Promega) and 330 ng of reporter plasmid encoding SBE-Firefly luciferase with Lipofectamine

(124) Structural insights into pathogenic mechanism of hypohidrotic ectodermal dysplasia caused by ectodysplasin A variantsNature communicationsFebruary 11, 2023Yu K, Huang C, Wan F, Jiang C, Chen J, Li X, Wang F, Wu J, Lei M, Wu Y.ng of pNF-κB Luc plasmid (Promega, E8491), and 10 ng of pRL‐TK Renilla reference plasmid (Promega, E2231) were co-transfected for each well using Lipofectamine 3000 (Invitrogen). Twenty-four hours after the

(125) Human IRF1 governs macrophagic IFN-γ immunity to mycobacteriaCellFebruary 2, 2023Rosain J, Neehus AL, Manry J, Yang R, Le Pen J, Daher W, Liu Z, Chan YH, Tahuil N, Türel Ö, et al.E6651 pGL4.10[luc2] backbon with five GGGAAAGTGAAACTA repeats,"Hernandez et al., 2018183",N/A pRL-SV40,Promega,Cat# E2231 lentiCRISPR v2,Addgene,Cat# 52961 psPAX2,Addgene,Cat# 12260 pCMV-VSV-G,Addgene,Cat# 8454 pHXB2-Env,NIH-AIDS

(126) THOR is a targetable epigenetic biomarker with clinical implications in breast cancerClinical EpigeneticsDecember 18, 2022Joana Dias Apolónio, João S. Dias, Mónica Teotónio Fernandes, Martin Komosa, Tatiana Lipman, Cindy H. Zhang, Ricardo Leão, Donghyun Lee, Nuno Miguel Nunes, Ana-Teresa Maia, et al.L3000015), according to manufacturer’s instructions. Control plasmid pRL Renilla luciferase vector (Promega, E2231) was co-transfected to normalize the readings. 24 h post-transfection the cells were lysed, and firefly

(127) Type 1 diabetes risk genes mediate pancreatic beta cell survival in response to proinflammatory cytokinesCell GenomicsDecember 14, 2022Paola Benaglio, Han Zhu, Mei-Lin Okino, Jian Yan, Ruth Elgamal, Naoki Nariai, Elisha Beebe, Katha Korgaonkar, Yunjiang Qiu, Margaret K.R. Donovan, et al.#12260 pGL4.23 Luciferase reporter vector,Promega,Cat#E8411 pRL-SV40 Renilla reporter vector,Promega,Cat#E2231 Lentiviral shSOCS1 (SHCLNG MISSION shRNA),Sigma-Aldrich,Cat#TRCN0000356244 ,, Software and algorithms,Software

(128) Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1) expression in glioblastoma is driven by ETS1- and MYBL2-dependent transcriptional activationCell Death DiscoveryDecember 1, 2022Laura Hauffe, Daniel Picard, Julian Musa, Marc Remke, Thomas G. P. Grünewald, Barak Rotblat, Guido Reifenberger, Gabriel LeprivierEIF4EBP1 promoter Firefly luciferase plasmid, 2 ng of Renilla luciferase-expressing pRL SV40 plasmid (E2231, Promega), as internal control, and 5–373 ng of either of the transcription factor expressing plasmids,

(129) PGC-1β modulates catabolism and fiber atrophy in the fasting-response of specific skeletal muscle bedsMolecular metabolismDecember 1, 2022Schmid S, Heim-Kupr B, Pérez-Schindler J, Mansingh S, Beer M, Mittal N, Ehrenfeuchter N, Handschin C.before adding to the cells. Cells were transfected 24 h after seeding with 5 ng pRL-SV40 (Promega, #E2231), 15 ng pNFAT-luc (Addgene, #17870), 10 ng pcDNA-NFAT, 40 ng pcDNA-f:PGC1b (Addgene, #1031). The total

(130) A SOX17-PDGFB signaling axis regulates aortic root developmentNature CommunicationsDecember 1, 2022Pengfei Lu et al.normalized by Renilla luciferase luminescence derived from the co-transfected pRL-SV40 vector (Promega, E2231). RNA-sequencing and quantitative real time reverse transcriptase reaction (RT-qPCR) Total RNAs were

(131) NF1 loss of function as an alternative initiating event in pancreatic ductal adenocarcinomaCell reportsNovember 8, 2022Ramakrishnan G, Parajuli P, Singh P, Friend C, Hurwitz E, Prunier C, Razzaque MS, Xu K, Atfi A.study,N/A pLVX-Hyg-PMLR,This study,N/A pGL3-NF1Luc (all mutants),This study,N/A pRL-SV40,Promega,E2231 pGL3-Basic,Promega,PR-E1751 pCMV-VSV-G,Addgene,8454 pUMVC,Addgene,8449 Flag-PML IV/pRK5,Addgene,59742 pLVX-IRES-Hyg-Smad4,Addgene,107128 pLVX-IRES-Neo,Takara,632181 pCMV5B-2xHA,Wrana

(132) LINC00589-dominated ceRNA networks regulate multiple chemoresistance and cancer stem cell-like properties in HER2(+) breast cancerNPJ breast cancerOctober 29, 2022Bai W, Peng H, Zhang J, Zhao Y, Li Z, Feng X, Zhang J, Liang F, Wang L, Zhang N, et al.sequences from LINC00589, DLG5, or PRDM16, together with pRL-SV40 Renilla luciferase vector (catalog number E2231, Promega) and miRNAs (miR-100 mimic, miR-452 mimic or negative control RNA) using Lipofectamine 2000

(133) Herpes Simplex Virus 1 Glycoproteins Differentially Regulate the Activity of Costimulatory Molecules and T CellsmSphereOctober 26, 2022Harry H. Matundan, Ujjaldeep Jaggi, Homayon Ghiasinamely, pGL4-CD80p, pGL4-CD86p, pGL4-CD4p, or pGL4-CD8p. pRL-SV40 (Promega, Madison, WI; catalog [cat.] E2231), a Renilla luciferase reporter plasmid, was used as a cotransfected internal control to monitor baseline

(134) Defective Mitochondrial Quality Control during Dengue Infection Contributes to Disease PathogenesisJournal of VirologyOctober 26, 2022Bharati Singh, Kiran Avula, Shamim Akhtar Sufi, Nahid Parwin, Sayani Das, Mohd Faraz Alam, Subhashish Samantaray, Leelakrishna Bankapalli, Alankrita Rani, Kokavalla Poornima, et al.alpha promoter 2-kb luciferase plasmid (Addgene no. 8887) and renilla luciferase plasmid (Promega no. E2231) were obtained from Addgene and Promega, respectively. The dengue 2 nonstructural protein constructs

(135) Antibody targeting of E3 ubiquitin ligases for receptor degradationNatureOctober 1, 2022Marei H, Tsai WK, Kee YS, Ruiz K, He J, Cox C, Sun T, Penikalapati S, Dwivedi P, Choi M, et al.transfected stably with a TOPbrite firefly luciferase Wnt reporter and pRL-SV40 Renilla luciferase (Promega, E2231) were maintained in RPMI 1640 media supplemented with 10% fetal bovine serum. For luciferase assay,

(136) Disease-associated inosine misincorporation into RNA hinders translationNucleic Acids ResearchSeptember 9, 2022Jacob H Schroader et al.parallel Renilla luciferase construct in the same backbone, the Renilla luciferase sequence (Promega E2231) was amplified by polymerase chain reaction (PCR) using the following primers: forward 5′-CATAGGATCCGCCACCATGGCTTCCAAG-3′

(137) Effect of Cisplatin on Renal Iron Homeostasis Components: Implication in NephropathyACS omegaAugust 16, 2022Aggarwal A, Dinda AK, Mukhopadhyay CK.the thymidine kinase promoter containing the Renilla luciferase reporter plasmid (pRL-TK, Promega, E2231). Cells were kept in the transfection cocktail containing both reporter vectors and Turbofect (Thermo Fischer Scientific

(138) Mettl3-mediated mRNA m(6)A modification controls postnatal liver development by modulating the transcription factor Hnf4aNature communicationsAugust 5, 2022Xu Y, Zhou Z, Kang X, Pan L, Liu C, Liang X, Chu J, Dong S, Li Y, Liu Q, et al.vectors with WT or mutated fragments, and 10 ng pRL Renilla Luciferase Control Reporter Vector (Promega, E2231) were co-transfected into HEK293T cells in triplicates in 96-well plates. Fluc and Rluc activities were

(139) CDK9 inhibition blocks the initiation of PINK1-PRKN-mediated mitophagy by regulating the SIRT1-FOXO3-BNIP3 axis and enhances the therapeutic effects involving mitochondrial dysfunction in hepatocellular carcinomaAutophagyAugust 1, 2022Yao J, Wang J, Xu Y, Guo Q, Sun Y, Liu J, Li S, Guo Y, Wei L.μg/well) (Addgene, 1789; deposited by Michael Greenberg) and pRL-TK Renilla (0.2 μg/well) (Promega, E2231) were transfected with Lipofectamine 2000 (Invitrogen, 11668019). Then, HepG2 cells were treated with

(140) Y RNAs are conserved endogenous RIG-I ligands across RNA virus infection and are targeted by HIV-1iScienceJuly 15, 2022Vabret N, Najburg V, Solovyov A, Gopal R, McClain C, Šulc P, Balan S, Rahou Y, Beauclair G, Chazal M, et al.study,N/A pISRE-Luc,Agilent (Stratagene),Cat: #219089 pIFNβ-Fluc (IFN-b-pGL3),Lin et al. (2000),N/A pTK-Rluc,Promega,Cat: #E2231 pCIneo,Promega,Cat: #E1841 pCMV-RNY4,This study,N/A pU6-RNY4,This study,N/A ,, Software and algorithms,Software

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

Citations (323)

Reviews

pRL-SV40 Vector from Promega

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