pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(201) cis-acting regulatory elements regulating CYP3A4 transcription in human liverPharmacogenet GenomicsJuly 1, 2020Joseph M. Collins, Danxin WangAnalytical Science, Waltham, MA, USA). As an internal control, Renilla luciferase constructs (Promega #E2231) driven by a TK promoter were co-transfected with promoter constructs at a 1:5 ratio. To test the effect

(202) Constitutive Expression of the Immunosuppressive Tryptophan Dioxygenase TDO2 in Glioblastoma Is Driven by the Transcription Factor C/EBPβFrontiers in ImmunologyMay 14, 2020Takumi Kudo, Mirja T. Prentzell, Soumya R. Mohapatra, Felix Sahm, Zhongliang Zhao, Ingrid Grummt, Wolfgang Wick, Christiane A. Opitz, Michael Platten, Edward W. Greenco-transfected with a constitutively active renilla luciferase-expressing plasmid (pRL-TK, Promega E2231) as a transfection control. Forty-eight hours after transfection, reporter assays were performed according

(203) Metabolic Reprogramming Promotes Neural Crest Migration via Yap/Tead SignalingDevelopmental cellApril 20, 2020Bhattacharya D, Azambuja AP, Simoes-Costa M.(Addgene plasmid # 83467) (Kim and Gumbiner, 2015) and the pRL-TK control reporter vector (Promega, #E2231). The embryos were then cultured ex ovo until HH9. The two dorsal neural folds of each embryo were micro-dissected,

(204) Identification of a new p53 responsive element in the promoter region of anillinInternational Journal of Molecular MedicineMarch 4, 2020Jiao Ma, Xinying Liu, Pengyi Liu, Wenqing Lu, Xinxin Shen, Ruixiang Ma, Hongliang ZongLaboratories, Inc.). Luciferase activity 1565 was normalized to Renilla luciferase activity (cat. no. E2231; Promega Corporation). Statistical analysis. An unpaired Student's t‑test was performed to compare the

(205) Identification and Characterization of Cannabimovone, a Cannabinoid from Cannabis sativa, as a Novel PPARγ Agonist via a Combined Computational and Functional StudyMolecules (Basel, Switzerland)March 3, 2020Iannotti FA, De Maio F, Panza E, Appendino G, Taglialatela-Scafati O, De Petrocellis L, Amodeo P, Vitale RM.TK-MH100 × 4-Luc containing the UAS enhancer elements, and (c) Renilla luciferase (pRL, Promega, Cat. E2231) using lipofectamine 2000 (cat. n. 11668027; Life Technologies; Milan, Italy) following the manufacturer’s

(206) Virus-Mediated Suppression of the Antigen Presentation Molecule MR1Cell reportsMarch 3, 2020McSharry BP, Samer C, McWilliam HEG, Ashley CL, Yee MB, Steain M, Liu L, Fairlie DP, Kinchington PR, McCluskey J, et al.M0252 MG132,Sigma-Aldrich,Cat# M7449 PS-341,Selleck Chemicals,Cat# S1013 Fugene HD,Promega,Cat# E2231 Polybrene,Sigma-Aldrich,Cat# TR-1003 folate free RPMI1640,Lonza,Cat# 12–702F Endo H,NEB,Cat# P0703S BglII,NEB,Cat#

(207) The transcription factor NFAT5 limits infection-induced type I interferon responsesThe Journal of experimental medicineMarch 2, 2020Huerga Encabo H, Traveset L, Argilaguet J, Angulo A, Nistal-Villán E, Jaiswal R, Escalante CR, Gekas C, Meyerhans A, Aramburu J, et al.8 µg of the indicated luciferase-expressing plasmids in each case and 2 µg of pRL-TK (Promega, Cat# E2231) as an internal control for electroporation efficiency. For shRNA transfection, 15 µg of shNFAT5 or

(208) β-Arrestin 2 mediates arginine vasopressin-induced IL-6 induction via the ERK_1/2-NF-κB signal pathway in murine heartsActa pharmacologica SinicaFebruary 1, 2020Sun SZ, Cao H, Yao N, Zhao LL, Zhu XF, Ni EA, Zhu Q, Zhu WZ.β-arrestin 2 was purchased from Genechem (Shanghai, China). NF-κB luciferase (E8491) and renilla luciferase (E2231) were purchased from Promega (Madison, MI, USA). Anti-P-NF-κB (#3033) and anti-β-arrestin 2 (4674S)

(209) Human Cytomegalovirus miRNAs Regulate TGF-β to Mediate Myelosuppression while Maintaining Viral Latency in CD34⁺ Hematopoietic Progenitor CellsCell host & microbeJanuary 8, 2020Hancock MH, Crawford LB, Pham AH, Mitchell J, Struthers HM, Yurochko AD, Caposio P, Nelson JA.oligonucleotide sequences,, Recombinant DNA,Recombinant DNA,Recombinant DNA pRL SV40-Rluc,Promega,Cat# E2231 pSiCheck2,Promega,PR-C8021 pSIREN-GFP,Clontech,Cat# 632455 SMAD Cignal Reporter Assay,Qiagen,Cat#

(210) USP7-Dependent Regulation of TRAF Activation and Signaling by a Viral Interferon Regulatory Factor HomologueJournal of virologyJanuary 6, 2020Xiang Q, Ju H, Nicholas J.respectively; deposited by D. Trono). The reporter pRL-Luc was obtained from Promega (catalogue number E2231). Expression plasmids pCEBZ-vIRF1-SF, pCEBZ-vIRF3-SF, pCEBZ-USP7-CBD, and pET22b-USP7-NTD were reported

(211) The interplay between non-esterified fatty acids and bovine peroxisome proliferator-activated receptors: results of an in vitro hybrid approachJournal of animal science and biotechnologyJanuary 1, 2020Busato S, Bionaz M.containing a PPRE-driven luciferase reporter, was used as a measure of PPAR activity. The plasmid pRL-TK (E2231, Promega, WI, USA), encoding Renilla reniformis luciferase guided by a minimal thymidine kinase promoter,

(212) Zhibaidihuang Decoction Ameliorates Cell Oxidative Stress by Regulating the Keap1-Nrf2-ARE Signalling PathwayEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2020Wu P, Ding B, Ye L, Huang Y, Ji J, Fan Y, Xu L.(Shanghai University of Traditional Chinese Medicine). The pRL Renilla Luciferase Control Reporter Vector (E2231) and the Dual-Luciferase® reporter assay system (E1910) were obtained from Promega (Madison, USA). dNTP

(213) Association of N⁶-methyladenine DNA with plaque progression in atherosclerosis via myocardial infarction-associated transcriptsCell death & diseaseDecember 4, 2019Wu L, Pei Y, Zhu Y, Jiang M, Wang C, Cui W, Zhang D.with the jetPEI-HUVEC DNA transfection reagent (Polyplus, USA) containing Renilla luciferase (Promega, E2231) bearing MIAT-pGl3-basic plasmids. Luciferase activity was assessed at 24 h after transfection by using

(214) Suppression of Ribosomal Pausing by eIF5A Is Necessary to Maintain the Fidelity of Start Codon SelectionCell ReportsDecember 3, 2019Hema Manjunath, He Zhang, Frederick Rehfeld, Jaeil Han, Tsung-Cheng Chang, Joshua T. Mendell#12259 pGL3-Control,Promega Corporation,Cat# E1741 pGL3-Control_NP2,This paper,N/A pRL-SV40,Promega Corporation,Cat# E2231 pcDNA3.1(+),Invitrogen,Cat# V790–20 Software and Algorithms,Software and Algorithms,Software and Algorithms Bowtie2,"Langmead

(215) KLF8 overexpression promotes the growth of human lung cancer cells by promoting the expression of JMJD2ACancer Cell InternationalDecember 1, 2019Dongjie Ma, Hongsheng Liu, Yingzhi Qin, Zhenhuan Tian, Shanqing Li, Naixin LiangThen, the related plasmids (pGL3-JMJD2A or pGL3-Ctrl) in combination with a pRL-TK plasmid (Promega, #E2231) were transfected in A549 cells for 24 h and luciferase assay was performed using a luciferase assay

(216) Transcriptional Regulation of ZNF638 in Thermogenic Cells by the cAMP Response Element Binding Protein in Male MiceJournal of the Endocrine SocietyDecember 1, 2019Perie L, Verma N, Xu L, Ma X, Mueller E.reporter vector (catalog no. E1751) and the pRL Renilla luciferase control reporter vector (catalog no. E2231) were purchased from Promega. The 2-kb sequence upstream of the transcription start site of the ZNF638

(217) High-throughput transcriptome analysis reveals that the loss of Pten activates a novel NKX6-1/RASGRP1 regulatory module to rescue microphthalmia caused by Fgfr2-deficient lensesHuman GeneticsDecember 1, 2019Stephanie L. Padula, Deepti Anand, Thanh V. Hoang, Blake R. Chaffee, Lin Liu, Chun Liang, Salil A. Lachke, Michael L. Robinsonused for cloning and transfection were pGL4.10[luc2] (New England Biolabs #E6651) pRL-TK (Promega #E2231), and Tet-O-FUW-Nkx6-1 (Addgene #45846). The putative promoter regions of mouse Rasgrp1 and IL-6 (2500bp

(218) Gm40600 suppressed SP 2/0 isograft tumor by reducing Blimp1 and Xbp1 proteinsBMC CancerDecember 1, 2019Ying Fang, Ruonan Xu, Bing Zhai, Chunmei Hou, Ning Ma, Liang Wang, Gencheng Han, Zhenyu Jiang, Renxi Wangpromoter (0.5 μg, cat# MPRM19639-PG04, Fugene Corp., Guangzhou, China), and pRLSV-40 vector (0.05 μg, cat# E2231, Promega Corp.) were co-transduced into 4 × 105 293 T cells according to our previous study [18]. In

(219) EPS8 Facilitates Uncoating of Influenza A VirusCell reportsNovember 19, 2019Larson GP, Tran V, Yú S, Caì Y, Higgins CA, Smith DM, Baker SF, Radoshitzky SR, Kuhn JH, Mehle A.pCAGGS-NP,"Neumann et al., 2005",N/A Plasmid: pHH21-vNA-Luc,"Regan et al., 2006",N/A Plasmid: pRL-SV40,Promega,E2231 Plasmid: psPAX2,D. Trono,Addgene 12260 Plasmid: pMD2.G,D. Trono,Addgene 12259 Software and Algorithms,Software

(220) Batf Pioneers the Reorganization of Chromatin in Developing Effector T Cells via Ets1-Dependent Recruitment of CtcfCell reportsOctober 29, 2019Pham D, Moseley CE, Gao M, Savic D, Winstead CJ, Sun M, Kee BL, Myers RM, Weaver CT, Hatton RD.2013",N/A pCMV6-Ets1,Origene,Cat#MR207015 MSCV-IRES-Thy1.1 Ets1,This paper,"N/A, generated in house" pRL-TK,Promega,Cat#E2231 pGL3-Il2p,This paper,"N/A, generated in house" pGL3-Il2p -35,This paper,"N/A, generated in house" pGL3-Il2p

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

Citations (323)

Reviews

pRL-SV40 Vector from Promega

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