pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) AMPK-HIF-1α signaling enhances glucose-derived de novo serine biosynthesis to promote glioblastoma growthJournal of experimental & clinical cancer research : CRDecember 15, 2023Yun HJ, Li M, Guo D, Jeon SM, Park SH, Lim JS, Lee SB, Liu R, Du L, Kim SH, et al.plasmid containing five copies of HREs (as an inner control that contained Renilla luciferase sequences (#E2231, Promega, Madison, WI)) using Lipofectamine2000 transfection reagent according to the manufacturer’s

(102) KLF12 transcriptionally regulates PD-L1 expression in non-small cell lung cancerMolecular oncologyDecember 1, 2023Pan X, Zhang W, Wang L, Guo H, Zheng M, Wu H, Weng Q, He Q, Ding L, Yang B.KLF12‐HA or KLF12 siRNA by using transfection reagent. The Renilla‐luciferase‐expressing plasmid (#E2231; Promega, Madison, WI, USA) was used as the internal control to normalize the transfection efficiency.

(103) Molecular basis of Wnt biogenesis, secretion, and Wnt7-specific signalingCellNovember 9, 2023Qi X, Hu Q, Elghobashi-Meinhardt N, Long T, Chen H, Li X.paper,N/A pEZT-BM,Addgene,Cat# 74099 pEZT-BM-WLS-Flag,This paper,N/A pRL-SV40 Renilla luciferase,Promega,Cat# E2231 pcDNA3.1,Invitrogen,Cat# V79020 pcDNA3.1-wnt7a-strep,This paper,N/A pcDNA3.1-wnt3a,This paper,N/A pcDNA3.1-FZD4,This

(104) CRISPR/Cas9 screen for genome‐wide interrogation of essential MYC‐bound E‐boxes in cancer cellsMolecular OncologyNovember 1, 2023Marta Kazimierska, Marta Podralska, Magdalena Żurawek, Tomasz Woźniak, Marta Elżbieta Kasprzyk, Weronika Sura, Wojciech Łosiewski, Iwona Ziółkowska‐Suchanek, Joost Kluiver, Anke van den Berg, et al.500 ng pGL4.23 vector with E‐box constructs and 5 ng pRL‐SV40 vector containing Renilla luciferase (#E2231, Promega). Twenty‐four hours after transfection, cells were lysed and luminescence was measured using

(105) ONECUT2 Activates Diverse Resistance Drivers of Androgen Receptor-Independent Heterogeneity in Prostate CancerbioRxivOctober 18, 2023Chen Qian, Qian Yang, Mirja Rotinen, Rongrong Huang, Hyoyoung Kim, Brad Gallent, Yiwu Yan, Radu M. Cadaneanu, Baohui Zhang, Salma Kaochar, et al.Luciferase reporter assay Cells were co-transfected with PGL4.10 luciferase backbone construct, and the pRL-SV40 vector (Promega #E2231) with Turbofectin 8.0 (Origene, #TF81001). After transfection overnight, cells were washed and treated

(106) Epigenomic perturbation of novel EGFR enhancers reduces the proliferative and invasive capacity of glioblastoma and increases sensitivity to temozolomideBMC CancerOctober 6, 2023Craig A. Vincent, Itzel Nissen, Soran Dakhel, Andreas Hörnblad, Silvia RemeseiroLipofectamine™ 2000 Transfection Reagent (Thermo Fisher) together with the pRL-SV40 vector (Promega Cat# E2231) for signal normalisation. pGL4.13[luc2/SV40] vector (Promega Cat# E6681) served as a positive control.

(107) Structural homology screens reveal host-derived poxvirus protein families impacting inflammasome activityCell reportsAugust 29, 2023Boys IN, Johnson AG, Quinlan MR, Kranzusch PJ, Elde NC.Addgene",12260 pUC19-muGFP_donor_A47,This study,N/A pUC19-muGFP_donor_C1,This study,N/A pET-SUMO2-EPTVgsdm,This study,N/A pRL-TK,Promega,E2231 pRS405,Laboratory of David Stillman,N/A pRS405-vvA47L,This study,N/A pRS405-GSDMD-CTD,This study,N/A pRS405-GFP,This

(108) Cry1Δ11 mutation induces ADHD-like symptoms through hyperactive dopamine D1 receptor signalingJCI insightAugust 22, 2023Liu D, Xie Z, Gu P, Li X, Zhang Y, Wang X, Chen Z, Deng S, Shu Y, Li JD.Luciferase reporter assay. The CRE-luciferase plasmid (Promega, E847A) and Renilla-luciferase plasmid (Promega, E2231) were transfected with the indicated plasmids into HEK293 cells. At 2 days after transfection, cells

(109) Picolinic acid is a broad-spectrum inhibitor of enveloped virus entry that restricts SARS-CoV-2 and influenza A virus in vivoCell reports. MedicineAugust 15, 2023Narayan R, Sharma M, Yadav R, Biji A, Khatun O, Kaur S, Kanojia A, Joy CM, Rajmani R, Sharma PR, et al.NP-Luciferase)","Kind Gift from prof. Adolfo Garcia-Sastre (ISMMS, NY)",Bortz et al.49 pRLTK,Promega,E2231 pWPI-IRES-Puro-Ak-ACE2-TMPRSS2,Gift from Sonja Best,Addgene plasmid # 154987; RRID:Addgene_154987) gag-pol,"Kind

(110) Characterization of molecular mechanisms driving Merkel cell polyomavirus oncogene transcription and tumorigenic potentialPLoS pathogensAugust 1, 2023Yang JF, Liu W, You J.from Warner Greene (Addgene 21984). pRL-SV40 (Renilla luciferase reporter) was purchased from Promega (E2231). Transfection and lentiviral transduction Lipofectamine 2000 (Invitrogen) was used for transient transfection

(111) An EZH2-NF-κB regulatory axis drives expression of pro-oncogenic gene signatures in triple negative breast canceriScienceJuly 21, 2023Dardis GJ, Wang J, Simon JM, Wang GG, Baldwin AS.RRID:Addgene_26699 pRP[Pro]-hRluc-{RELB_1.7kb},This manuscript,VectorBuilder ID VB220112-1274kts pRL-TK-Renilla,Promega,Cat# E2231 Lenti-pCDH-TetOn-shEV,Laboratory of Dr. Greg Wang (UNC Chapel Hill)31,N/A Lenti-pCDH-TetOn-shEZH2,Laboratory

(112) Time-dependent recruitment of GAF, ISGF3 and IRF1 complexes shapes IFNα and IFNγ-activated transcriptional responses and explains mechanistic and functional overlapCellular and Molecular Life SciencesJuly 1, 2023Agata Sekrecka, Katarzyna Kluzek, Michal Sekrecki, Mahdi Eskandarian Boroujeni, Sanaz Hassani, Shota Yamauchi, Kiyonao Sada, Joanna Wesoly, Hans A. R. BluyssenSimultaneously, as a reference, 30 ng of pRL Renilla Luciferase Control Reporter Vector (pRL-SV40; [E2231, Promega]) was co-transfected to the cells. After 24 h of incubation, IFNα (1000 U/ml) or IFNγ (10 ng/ml)

(113) Notch1 promotes resistance to cisplatin by up-regulating Ecto-5′-nucleotidase (CD73) in triple-negative breast cancer cellsCell Death DiscoveryJune 30, 2023Yuzhu Qi, Meifang Li, Shaozhong Li, De Zeng, Yingsheng Xiao, Jiwei Li, Qianqian Ye, Edwin Bremer, Guo-jun Zhanggradient manner with LV201-SH, LV201-N1ICD, siNocth1, siNC, and pRL-SV40 (Promega Madison, WI, USA; E2231). After a total of 48 h of treatment, the luciferase activity was measured by using ONE_Glo EX Dual-Luciferase Assay System

(114) PKM2 dictates the poised chromatin state of PFKFB3 promoter to enhance breast cancer progressionNAR CancerJune 28, 2023Madhura R Pandkar, Adarsh Raveendran, Kajal Biswas, Srinivas Abhishek Mutnuru, Jharna Mishra, Atul Samaiya, Tyler Malys, Alexander Y Mitrophanov, Shyam K Sharan, Sanjeev Shuklawith different PFKFB3-HRE luciferase constructs along with pRL-TK renilla luciferase plasmid (Promega, E2231). 12 h post-transfection, the cells were subjected to appropriate treatments for 12 h. Subsequently,

(115) STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer ProgressionInternational journal of molecular sciencesJune 12, 2023Lin Z, Liu Y, Xu T, Su T, Yang Y, Liang R, Gu S, Li J, Song X, Liang B, et al.were co-transfected with pGL3-derived plasmids and the internal control plasmid pRL-SV40 (Promega, E2231) at a 100:1 ratio in 100 ng total DNA per well. Luciferase activity was determined by dual-luciferase reporter assay

(116) A neomorphic mutation in the interferon activation domain of IRF4 causes a dominant primary immunodeficiencyThe Journal of experimental medicineJune 5, 2023Thouenon R, Chentout L, Moreno-Corona N, Poggi L, Lombardi EP, Hoareau B, Schmitt Y, Lagresle-Peyrou C, Bustamante J, André I, et al.transfected with the (ISRE)3 reporter plasmid (100 ng/well in a 96-well plate), the pRL-SV40 vector (# E2231, 40 ng/well; Promega), and an IRF4 WT or mutant pcDNA 3.1D/V5-His-TOPO plasmid (#K4900-01; Invitrogen,

(117) Interaction between poly(A)-binding protein PABPC4 and nuclear receptor corepressor NCoR1 modulates a metabolic stress responseThe Journal of biological chemistryJune 1, 2023Oliveira AG, Oliveira LD, Cruz MV, Guimarães DSPSF, Lima TI, Santos-Fávero BC, Luchessi AD, Pauletti BA, Leme AP, Bajgelman MC, et al.previously described, with 250 ng of pPPRE X3-TK-luc (Addgene, #1015) and 25 ng of pRL-SV40 (Promega, #E2231). After 48 h, the cells were lysed with 100 μl of 1 × passive lysis buffer (Promega, #E194A), and luciferase

(118) Mevalonate improves anti-PD-1/PD-L1 efficacy by stabilizing CD274 mRNAActa pharmaceutica Sinica. BJune 1, 2023Zhang W, Pan X, Xu Y, Guo H, Zheng M, Chen X, Wu H, Luan F, He Q, Ding L, et al.system (#E1910, Promega, USA) following the manufacturer's protocol. The Renilla luciferase plasmid (#E2231, Promege, USA) was used as the internal control to normalize the transfection efficiency, and the results

(119) A non‐translational role of threonyl‐tRNA synthetase in regulating JNK signaling during myogenic differentiationThe FASEB JournalMay 17, 2023Chong Dai, Adriana Reyes‐Ordoñez, Jae‐Sung You, Jie Chenobtained from Addgene: pCMV5-MEKK4alpha (#12187) and M50 Super 8 × TOPFlash (#12456). pRL-SV40 (Cat #: E2231) was from Promega. 2.3 | Cell culture C2C12 myoblasts were a gift from S. Kaufman at the University

(120) Tumor heterogeneity in VHL drives metastasis in clear cell renal cell carcinomaSignal Transduction and Targeted TherapyApril 17, 2023Junhui Hu, Ping Tan, Moe Ishihara, Nicholas A. Bayley, Shiruyeh Schokrpur, Jeremy G. Reynoso, Yangjun Zhang, Raymond J. Lim, Camelia Dumitras, Lu Yang, et al.CCGCTCGAGTTGAGAAGAACGAGAGTAGAGATTTTAGG. The control renilla luciferase vector was pRL-TK from Promega (CA, USA, catalog number: E2231). The plasmid for overexpressing constitutively-active HIF1A was from Addgene (MA, USA, catalog number:

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

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Reviews

pRL-SV40 Vector from Promega

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