pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(281) Differential Expression of HERV-K (HML-2) Proviruses in Cells and Virions of the Teratocarcinoma Cell Line Tera-1VirusesMarch 4, 2015Neeru Bhardwaj, Meagan Montesion, Farrah Roy, John M. Coffininterest, was co-transfected alongside a pRL-SV40 internal control Renilla luciferase vector (Promega, Cat# E2231) at a 30:1 ratio, as recommended by the manufacturer’s protocol. Non-transfected Tera-1 cells were used

(282) Spatially restricted Hedgehog signalling regulates HGF-induced branching of the adult prostateNature cell biologyDecember 1, 2014Lim A, Shin K, Zhao C, Kawano S, Beachy PA.expression construct containing the 3′UTR of murine Hgf, 20 ng pRL-SV40 Renilla luciferase vector (Promega E2231) and 100 nM miR-26a mirVana miRNA mimic (Ambion, Assay MC10249) or miR-26b mimic (Ambion, Assay MC12899)

(283) Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cellsCardiovascular diabetologyFebruary 12, 2014Jung CH, Lee MJ, Kang YM, Lee YL, Yoon HK, Kang SW, Lee WJ, Park JY.CA, USA), as previously described [21]. pRL-SV40 renilla luciferase control reporter vector (10 ng, E2231, Promega, Madison, WI, USA) was co-transfected as an internal control. Twenty-four hours after transfection,

(284) Tristetraprolin mediates anti-inflammatory effect of carbon monoxide against DSS-induced colitisPloS oneJanuary 1, 2014Joe Y, Uddin MJ, Zheng M, Kim HJ, Chen Y, Yoon NA, Cho GJ, Park JW, Chung HT.the pGL3 basic vector (Promega). A pRL-SV40 Renilla luciferase construct was purchased from Promega (E2231). RAW264.7 cells were transfected with luciferase reporter constructs using the Neon™ transfection system

(285) Tumor suppressor p53 plays a key role in induction of both tristetraprolin and let-7 in human cancer cellsNucleic acids researchJune 1, 2013Lee JY, Kim HJ, Yoon NA, Lee WH, Min YJ, Ko BK, Lee BJ, Lee A, Cha HJ, Cho WJ, et al.mutant) constructs were purchased from Clontech (631922), and pGL3 basic and pRL-SV40 from Promega (E2231). Small interfering RNAs (siRNAs) against human TTP (TTP-siRNA, sc-36761), human Lin28a (Lin28a-siRNA,

(286) The Corepressor NCoR1 Antagonizes PGC-1α and Estrogen-Related Receptor α in the Regulation of Skeletal Muscle Function and Oxidative MetabolismMolecular and Cellular BiologyDecember 15, 2012Joaquín Pérez-Schindler, Serge Summermatter, Silvia Salatino, Francesco Zorzato, Markus Beer, Piotr J. Balwierz, Erik van Nimwegen, Jérôme N. Feige, Johan Auwerx, Christoph Handschinsimian virus 40 (SV40) promoters. Cells were transfected using Lipofectamine 2000 (Invitrogen) with 0.1 ␮g pRL-SV40 (E2231; Promega), 0.3 ␮g pPPRE X3TK-luc (1015; Bruce Spiegelman; Addgene), 0.3 ␮g pERRE-luc (gift of Junichi

(287) Isoform-selective induction of human p110δ PI3K expression by TNFα: identification of a new and inducible PIK3CD promoterThe Biochemical journalMay 1, 2012Whitehead MA, Bombardieri M, Pitzalis C, Vanhaesebroeck B.signals. Cloning of pGL3 reporter constructs pGL3-Basic (E1751), pGL3-SV40 (E1761), pRL-SV40 (Renilla; E2231) were from Promega. Regions surrounding human PIK3CD exons − 2a and − 2e ( − 139 to + 92 and − 385 to

(288) The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cellsBMC Molecular BiologyDecember 31, 2010Meesbah Jiwaji, Rónán Daly, Kshama Pansare, Pauline McLean, Jingli Yang, Walter Kolch, Andrew R Pittunless otherwise stated, a total of 100 ng DNA (50 ng pRL-SV40 (Promega Corporation, Madison, WI, USA; E2231) and 50 ng of either pMN85 or pMN86) was used in each transfection. HEK293 cells were transfected using

(289) Myoferlin regulation by NFAT in muscle injury, regeneration and repairJournal of cell scienceJuly 15, 2010Demonbreun AR, Lapidos KA, Heretis K, Levin S, Dale R, Pytel P, Svensson EC, McNally EM.(Invitrogen), 10 ml Plus reagent (Invitrogen) and 50 ng of pRLSV40 Renilla luciferase plasmid (Promega, E2231) in Opti-MEM. The pGL2-basic vector or the pGL2-SV40 control vector (Promega, E1631) replaced the reporter vector

(290) Differential repair of polycyclic aromatic hydrocarbon DNA adducts from an actively transcribed geneDNA RepairJune 10, 2010Qing Zhong, Shantu Amin, Philip Lazarus, Thomas E. Spratt

(291) Roles for common MLL/COMPASS subunits and the 19S proteasome in regulating CIITA pIV and MHC class II gene expression and promoter methylationEpigenetics & chromatinFebruary 4, 2010Koues OI, Mehta NT, Truax AD, Dudley RK, Brooks JK, Greer SF.HLA-DRA-Luc, pcDNA3 plasmids were as previously described [49]. Renilla luciferase control vector (E2231) was from Promega (Madison, WI, USA). Myc-Sug1 was generously provided by A. A. Wani [50]. ChIP assays ChIP

(292) The 19S ATPase S6a (S6'/TBP1) Regulates the Transcription Initiation of Class II TransactivatorJournal of Molecular BiologyOctober 14, 2009Agnieszka D. Truax, Olivia I. Koues, Marta K. Mentel, Susanna F. Greer

(293) A single nucleotide polymorphism in the 3′UTR of the SNCA gene encoding alpha-synuclein is a new potential susceptibility locus for Parkinson diseaseNeuroscience LettersSeptember 18, 2009Sotiria Sotiriou, Gretchen Gibney, Andreas D. Baxevanis, Robert L. NussbaumCRL-2266) were transfected with 1 ug of luciferase construct and 100 ng of Renilla plasmid (Promega E2231) using FuGENE (Roche 11-814-443-001). Cell lysates were analyzed using the dual luciferase assay kit

(294) Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryosBMC BiotechnologyDecember 1, 2008Francisca Alcaraz-Pérez, Victoriano Mulero, María L Cayuelareporter plasmids from Promega: pRL-CMV (Cat.# E2261), pRL-TK (Cat.# E2241) and/or pRL-SV40 (Cat.# E2231) (Table 1). • Adult zebrafish. • Egg water (60 mg/l Ocean Salt, 0.45 mM NaHCO3, 0.0375 mM CaCO3, 0.05%

(295) Evaluating cell-surface expression and measuring activation of mammalian odorant receptors in heterologous cellsNature ProtocolsSeptember 6, 2008Hanyi Zhuang, Hiroaki Matsunami25030-081) • pCRE-Luc expression construct (Stratagene 219076) • pSV40-Renilla expression construct (Promega E2231) • • Mammalian Ric8b construct cloned into a mammalian expression vector such as pCI (GENBANK accession

(296) FGFR1 inhibits skeletal muscle atrophy associated with hindlimb suspensionBMC Musculoskeletal DisordersDecember 1, 2007John Eash, Aaron Olsen, Gert Breur, Dave Gerrard, Kevin Hannonluciferase vector containing the SV40 promoter region (pRL-SV40) was purchased from Promega (catalog no. E2231, Madison, WI). Plasmid DNA encoding for Runx2/Osf2 under the control of a cytomegalovirus promoter (pCMV-Osf2)

(297) Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) transcriptional regulation by Oct-1 in human endothelial cells: implications for atherosclerosisBiochemical JournalJanuary 1, 2006Jiawei Chen, Yong Liu, Hongmei Liu, Paul L. Hermonat, Jawahar L. Mehtainternal control for transfection efficiency, 1 µg of Renilla luciferase vector (pRL-SV40, Promega catalogue no. E2231; SV40 stands for simian virus 40) was co-transfected. The transfection reagent was removed 6 h after

(298) "Unbiased discovery of interactions at a control locus driving expression of the cancer-specific therapeutic and diagnostic target, mesothelin"Journal of proteome researchJanuary 1, 0001"Ren YR, Chaerkady R, Hu S, Wan J, Qian J, Zhu H, Pandey A, Kern SE."from IDT) to make double-stranded DNA. Plasmids The pRL-SV40 plasmids were obtained from Promega (E2231). pGL3-Can3 was described previously.8 TEAD1, YAP1 and ZNF24 sequences were inserted into pcDNA3.1-FLAG

(299) Loss of the wild-type KRAS allele promotes pancreatic cancer progression through functional activation of YAP1OncogeneJanuary 1, 0001"Yan H, Yu CC, Fine SA, Youssof AL, Yang YR, Yan J, Karg DC, Cheung EC, Friedman RA, Ying H, et al."anti-flag immunoblots. For reporter assay, pRL-SV40 Renilla luciferase vector (Promega, Catalog #: E2231) was co-transfected with HOP-FLASH/HIP-FLASH into cells in 96-well plates in 6 replicates in three independent

(300) VAV2 signaling promotes regenerative proliferation in both cutaneous and head and neck squamous cell carcinomaNature communicationsJanuary 1, 0001"Lorenzo-Martín LF, Fernández-Parejo N, Menacho-Márquez M, Rodríguez-Fdez S, Robles-Valero J, Zumalave S, Fabbiano S, Pascual G, García-Pedrero JM, Abad A, et al."from Addgene, Catalog No. 34615), and pRL-SV40 (Renilla luciferase, obtained from Promega, Catalog No. E2231). All plasmids were DNA sequence verified. In silico analyses of gene expression data from human tumors All

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

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Promega

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pRL-SV40 Vector from Promega

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