pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(301) Epigenetic silencing of SALL2 confers tamoxifen resistance in breast cancerEMBO molecular medicineJanuary 1, 0001"Ye L, Lin C, Wang X, Li Q, Li Y, Wang M, Zhao Z, Wu X, Shi D, Xiao Y, et al."luciferase reporter plasmids or the control luciferase plasmid, plus 1 ng pRL‐TK Renilla plasmid (#E2231; Promega), was transfected into cells using the Lipofectamine 3000 reagent (#L3000015; Invitrogen) according

(302) CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activityeLifeJanuary 1, 0001"Park MH, Kim AK, Manandhar S, Oh SY, Jang GH, Kang L, Lee DW, Hyeon DY, Lee SH, Lee HE, et al."plasmid Prof. Young Geun Kwon Yonsei University Recombinant DNA reagent pRL-SV40 plasmid Promega E2231 Sequencebased reagent siRNA:siCCN1 Qiagen SI03053477 SI03028655 SI02626428 SI02626421 Sequencebased reagent siRNA: siIntegrin

(303) Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3cDevelopmental cellJanuary 1, 0001"Fujii K, Zhulyn O, Byeon GW, Genuth NR, Kerr CH, Walsh EM, Barna M."paper,N/A ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, ,, Recombinant DNA,Recombinant DNA,Recombinant DNA SV40_Renilla-luc,Promega,E2231 pK429_pGL1_Ptch1_WT,"(Fujii et al., 2017)",N/A pK610_pGL54_Ptch1_ΔBS,This paper,N/A pK626_pGL70_Ptch1_ΔUC,This

(304) The 19S proteasome positively regulates histone methylation at cytokine inducible genesBiochimica et biophysica actaJanuary 1, 0001"Koues OI, Dudley RK, Mehta NT, Greer SF."

(305) HDAC11 activity contributes to MEK inhibitor escape in uveal melanomaCancer gene therapyJanuary 1, 0001"Sriramareddy SN, Faião-Flores F, Emmons MF, Saha B, Chellappan S, Wyatt C, Smalley I, Licht JD, Durante MA, Harbour JW, et al."plasmid, 8xGTIIC-luciferase (Addgene, #34615) and control plasmid, pRL Renilla luciferase from Promega (#E2231) were used in this assay. 92.1, Mel270 and MP41 cells (120 × 103 cells per well in 6-well plates) were

(306) Transcript-specific induction of stop codon readthrough using a CRISPR-dCas13 systemEMBO reportsJanuary 1, 0001"Manjunath LE, Singh A, Devi Kumar S, Vasu K, Kar D, Sellamuthu K, Eswarappa SM."study, pSpCas9(BB)-2A-Puro,Addgene,#48139 pSpCas9(BB)-2A-GFP,Addgene,#48138 pcDNA3.1B,Invitrogen,V800-20 pRL-SV40,Promega,E2231 pmCherry-c1,"A kind gift from Prof. P.N. Rangarajan Lab, Department of Biochemistry, IISc", Sequence-based

(307) Identifying the Transcriptional Drivers of Metastasis Embedded within Localized MelanomaCancer discoveryJanuary 1, 0001Suresh S, Rabbie R, Garg M, Lumaquin D, Huang TH, Montal E, Ma Y, Cruz NM, Tang X, Nsengimana J, et al.and Renilla luciferase plasmid (pRL-SV40, RRID Addgene_27163) were obtained from Promega (E1751 and E2231) and pGL3-AP-1 was from Addgene (RRID Addgene_40342). Details of the oligonucleotide sequences used

(308) Occludin Regulates HIV-1 Infection by Modulation of the Interferon Stimulated OAS Gene FamilyMolecular neurobiologyJanuary 1, 0001"Torices S, Teglas T, Naranjo O, Fattakhov N, Frydlova K, Cabrera R, Osborne OM, Sun E, Kluttz A, Toborek M."WI, USA, Cat# E414A), and 0.5 μg of the pRL Renilla Luciferase Control Reporter Vector (Promega, Cat# E2231). Cell lysates were analyzed using the dual-luciferase reporter assay (Promega, Cat# E1910). Renilla

(309) Differential regulation of alternate promoter regions in Sox17 during endodermal and vascular endothelial developmentiScienceJanuary 1, 0001"Trinh LT, Osipovich AB, Sampson L, Wong J, Wright CVE, Magnuson MA."DNA,Recombinant DNA,Recombinant DNA ,, pGL4.14[luc2/Hygro],Promega,Cat# E6691 pRL-SV40,Promega,Cat# E2231 pGL2-Control vector,Promega,Cat# E1611 Sox17 BAC (RPCI-22),BACPAC (CHORI),Index# 49I6 ,, Software

(310) Intragenic Enhancers Attenuate Host Gene ExpressionMolecular cellJanuary 1, 0001"Cinghu S, Yang P, Kosak JP, Conway AE, Kumar D, Oldfield AJ, Adelman K, Jothi R."paper,N/A Plasmid: pGL3-Basic,Promega,E1751 Plasmid: pIntragenic,This paper,N/A Plasmid: pRL-TK,Promega,E2231 Plasmid: pTN23,Prof. I.C. Eperon,N/A Plasmid: pX330-U6-Chimeric_BB-CBh-hSpCas9,Addgene,42230 Publicly

(311) O-GlcNAcylation regulates OTX2's proteostasisiScienceJanuary 1, 0001"Wulff-Fuentes E, Boakye J, Kroenke K, Berendt RR, Martinez-Morant C, Pereckas M, Hanover JA, Olivier-Van Stichelen S."France)", OTX2(S135A/S136A/T137A)-GFP,"T. Lamonerie, PHD (IBV, France)", IRBP-SEAP,"T. Lamonerie, PHD (IBV, France)", pRL,"Promega, E2231", ,, Software and algorithms,Software and algorithms,Software and algorithms ,, AlphaFold,, PyMOL,, Cytoscape,, STRING,, AGGRESCAN,, Biorender,, GraphPad

(312) Obesity-induced metabolic imbalance allosterically modulates CtBP2 to inhibit PPAR-alpha transcriptional activityThe Journal of biological chemistryJanuary 1, 0001"Saito K, Sekiya M, Kainoh K, Yoshino R, Hayashi A, Han SI, Araki M, Ohno H, Takeuchi Y, Tsuyuzaki T, et al."ng of a PPRE luciferase reporter plasmid and 5 ng of a pRL-SV40 plasmid encoding Renilla (Promega, E2231) using Lipofectamine LTX with Plus Reagent. For overexpression, cells were cotransfected with 100 ng

(313) Constitutive GLI1 expression in chondrosarcoma is regulated by major vault protein via mTOR/S6K1 signaling cascadeCell death and differentiationJanuary 1, 0001"Wang W, Yan T, Guo W, Niu J, Zhao Z, Sun K, Zhang H, Yu Y, Ren T."12-well plate and transfected with 1 μg luciferase reporter plasmids and 500 ng of pRL-TK plasmids (E2231, Promega Madison, WI) using Lipofectamine 3000. Forty-eight hours after transfection and treatment,

(314) BCL9 provides multi-cellular communication properties in colorectal cancer by interacting with paraspeckle proteinsNature communicationsJanuary 1, 0001"Jiang M, Kang Y, Sewastianik T, Wang J, Tanton H, Alder K, Dennis P, Xin Y, Wang Z, Liu R, et al."with NFAT luciferase reporter (Plasmid #10959, Addgene) and SV40 drive renilla luciferase reporter (E2231, Promega). 24 h post-transfection, 1 × 105 cells were plated per well in 96-well plates. 24 h after

(315) TAZ maintains telomere length in TNBC cells by mediating Rad51C expressionBreast cancer research : BCRJanuary 1, 0001"Yang L, Wang B, Jiao X, Zhou C, Chen S, Gao X, Sun W, Song S, Li J, Liu J, et al."plasmids were transfected into cells together with pRL-SV40 Renila Luciferase Control Reporter Vectors (#E2231, Promega) by using Lipofectamine 2000 (#11668-019, Invitrogene). 48 h after the transfection, the cell

(316) Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in HypoxiaCell reportsJanuary 1, 0001"Chowdhury A, Aich A, Jain G, Wozny K, Lüchtenborg C, Hartmann M, Bernhard O, Balleiniger M, Alfar EA, Zieseniss A, et al."luciferase reporter,"Wanner et al., 2000",N/A Plasmid: pRLSV40 Renilla luciferase reporter,Promega,Cat#E2231 Plasmid: pNF-κB-Luc,Clontech,Cat#631743 ,, Software and Algorithms,Software and Algorithms,Software

(317) Occludin regulates HIV-1 infection by modulation of the interferon stimulated OAS gene familyResearch squareJanuary 1, 0001"Torices S, Teglas T, Naranjo O, Fattakhov N, Frydlova K, Cabrera R, Osborne OM, Sun E, Kluttz A, Toborek M."WI, USA, Cat# E414A), and 0.5 μg of the pRL Renilla Luciferase Control Reporter Vector (Promega, Cat# E2231). Cell lysates were analyzed using the dual-luciferase reporter assay (Promega, Cat# E1910). Renilla

(318) "EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion"CellJanuary 1, 0001"Marzio A, Kurz E, Sahni JM, Di Feo G, Puccini J, Jiang S, Hirsch CA, Arbini AA, Wu WL, Pass HI, et al."17654 pGL4.45[luc2P/ISRE/Hygro],Promega,cat. No. 9PIE414 pRL Renilla Luciferase,Promega,cat. No. E2231 pcDNA3.1 2xFLAG-2xSTREP-Emsy (mouse),Michele Pagano Laboratory,N/A pcDNA3.1 2xFLAG-2xSTREP-EMSY (human),Michele

(319) SOX9 is a key component of RUNX2-regulated transcriptional circuitry in osteosarcomaCell & bioscienceJanuary 1, 0001"Kim YI, Tseng YC, Ayaz G, Wang S, Yan H, du Bois W, Yang H, Zhen T, Lee MP, Liu P, et al."empty vector or a vector expressing RUNX2 plus 20 ng of pRL-SV40 (an internal control, Promega, Cat: E2231). Both firefly luciferase and Renilla luciferase activities were measured using Dual-Glo® Luciferase Assay System

(320) MRTF-A regulates myoblast commitment to differentiation by targeting PAX7 during muscle regenerationJournal of cellular and molecular medicineJanuary 1, 0001"Song R, Zhao S, Xu Y, Hu J, Ke S, Li F, Tian G, Zheng X, Li J, Gu L, et al."were seeded in a 48‐well plate, and 400 ng total plasmids containing pGL3‐CArG and pRL‐TK (cat. no. E2231, Promega, Madison, WI) were transfected using Lipfectamine™ 3000 reagent (cat. no. L3000015, Invitrogen,

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

Citations (323)

Reviews

pRL-SV40 Vector from Promega

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