pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(261) Neurodegeneration-associated FUS is a novel regulator of circadian gene expressionTranslational neurodegenerationJanuary 1, 2018Jiang X, Zhang T, Wang H, Wang T, Qin M, Bao P, Wang R, Liu Y, Chang HC, Yan J, et al.transfected with firefly luciferase reporter with renilla luciferase reporter (pRL-SV40 Vector, Promega, E2231) as internal control. According to the technical manual of Dual-Luciiferase Reporter Assay System (Promega,

(262) Mechanisms of HERV-K (HML-2) Transcription during Human Mammary Epithelial Cell TransformationJournal of VirologyJanuary 1, 2018Meagan Montesion, Neeru Bhardwaj, Zachary H. Williams, Charlotte Kuperwasser, John M. CoffinLTR-containing pGL4 vector together with a pRL-SV40 internal control Renilla luciferase vector (catalog number E2231; Promega) at a 30:1 ratio. Transfections were carried out using the Opti-MEM reduced-serum medium (catalog

(263) Salidroside Inhibits Myogenesis by Modulating p-Smad3-Induced Myf5 TranscriptionFrontiers in pharmacologyJanuary 1, 2018Zhang P, Li W, Wang L, Liu H, Gong J, Wang F, Chen X.assays, cells were co-transfected with 500 ng of reporter plasmids, 25 ng of pRL-TK vectors (Promega, E2231) as an internal control. Luciferase activity was analyzed using the dual-luciferase reporter assay system

(264) Structural Basis for Regulated Proteolysis by the α-Secretase ADAM10CellDecember 14, 2017Tom C.M. Seegar et al.paper, pSpCas9(BB)-2A-GFP (PX458),Addgene,48138 pSpCas9(BB) adam10exon1-2A-GFP,This paper, pRL Renilla Luciferase,Promega,E2231 Gal4-firefly Luciferase,"Aster et al., 2000", Notch1-gal4,"Andrawes et al., 2013", pcDNA3-ΔProMP-humanADAM17-HAtag,Addgene,65221 Software

(265) REST regulates the cell cycle for cardiac development and regenerationNature communicationsDecember 7, 2017Zhang D, Wang Y, Lu P, Wang P, Yuan X, Yan J, Cai C, Chang CP, Zheng D, Wu B, et al.of each p21-RE1-pGL3 enhancer construct was co-transfected with 0.2 µg of pRL-SV40 vector (Promega, E2231) into cultured P1 CMs in 96-well plates using Lipofectamine 3000 Reagents (Invitrogen). After 24 h transfection,

(266) Bladder-cancer-associated mutations in RXRA activate peroxisome proliferator-activated receptors to drive urothelial proliferationeLifeNovember 16, 2017Halstead AM, Kapadia CD, Bolzenius J, Chu CE, Schriefer A, Wartman LD, Bowman GR, Arora VK.16818722,Addgene:13458,plasmid was deposited by T. Michael Underhill recombinant DNA reagent,pRL-SV40 (plasmid),Promega,Promega:E2231, recombinant DNA reagent,pCL-ampho (plasmid),other,,obtained from Dr. Charles Sawyers (MSKCC) recombinant

(267) A Paradoxical Tumor-Suppressor Role for the Rac1 Exchange Factor Vav1 in T Cell Acute Lymphoblastic LeukemiaCancer cellNovember 13, 2017Robles-Valero J, Lorenzo-Martín LF, Menacho-Márquez M, Fernández-Pisonero I, Abad A, Camós M, Toribio ML, Espinosa L, Bigas A, Bustelo XR.et al., 2003)",N/A pHES1-Luc,"(Espinosa et al., 2003)",N/A pRL-SV40 (Renilla vector),Promega,Cat# E2231 pMSCV-GFP retroviral vector,Dr. A. Ferrando Lab,N/A pMSCV-GFP-TLX1 retroviral vector,Dr. A. Ferrando

(268) Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating neviNature communicationsSeptember 21, 2017Yeh I, Lang UE, Durieux E, Tee MK, Jorapur A, Shain AH, Haddad V, Pissaloux D, Chen X, Cerroni L, et al.from Randall Moon, Addgene plasmid, #12456)46 and renilla control reporter (pRL-CMV Vector, Promega, E2231). At 24 h after transfection, luciferase activity was assayed in 10 μl of lysate using the Dual-Luciferase reporter assay system

(269) GRK2 Mediates Arginine Vasopressin-Induced Interleukin-6 Production via Nuclear Factor-κB Signaling Neonatal Rat Cardiac FibroblastMolecular pharmacologySeptember 1, 2017Xu F, Sun S, Wang X, Ni E, Zhao L, Zhu W.from Dr. Walter Koch (Temple University, Philadelphia, PA). Both NF-kB luciferase (E8491) and Renilla (E2231) were purchased from Promega (Madison, WI). Anti- P-NF-kB(S536) antibody was from Cell Signaling Technology

(270) Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse heartsNucleic acids researchApril 7, 2017Zhang D, Wu B, Wang P, Wang Y, Lu P, Nechiporuk T, Floss T, Greally JM, Zheng D, Zhou B.normalized by Renilla luciferase luminescence derived from the co-transfected pRL-SV40 vector (Promega, E2231). Electrophoretic mobility shift assay (EMSA) An EMSA kit (Thermos Scientific, 20148) was used to

(271) Cell-Cell Contact Area Affects Notch Signaling and Notch-Dependent PatterningDevelopmental cellMarch 13, 2017Shaya O, Binshtok U, Hersch M, Rivkin D, Weinreb S, Amir-Zilberstein L, Khamaisi B, Oppenheim O, Desai RA, Goodyear RJ, et al.paper,N/A pEV-UAS-H2B-mCherry,This paper,N/A Gal4-firefly luciferase reporter,"Andrawes et al., 2013",N/A pRL-SV40,Promega,E2231 Software and Algorithms,Software and Algorithms,Software and Algorithms MATLAB,MathWorks,R2016 Code

(272) Hyperhomocysteinemia results from and promotes hepatocellular carcinoma via CYP450 metabolism by CYP2J2 DNA methylationOncotargetFebruary 28, 2017Zhang D, Lou J, Zhang X, Zhang L, Wang F, Xu D, Niu N, Wang Y, Wu Y, Cui W.experiment according to the manufacturer's instructions. The dual-luciferase reporter assay (Promega, E2231) was performed after 24 h in accordance with the manufacturer's instructions. siRNA knockdown and transfection The

(273) pH, Lactate, and Hypoxia: Reciprocity in Regulating High-Affinity Monocarboxylate Transporter Expression in Glioblastoma1NeoplasiaFebruary 1, 2017James P. Caruso, Brandon J. Koch, Philip D. Benson, Elsa Varughese, Michael D. Monterey, Amy E. Lee, Ajal M. Dave, Sam Kiousis, Andrew E. Sloan, Saroj P. MathupalaTS667 cell lines. These optimum ratios were identified by transfecting each cell line with pRL-SV40 (#E2231, Renilla luciferase control vector; Promega) per manufacturer's (Roche) instructions. Fugene-6 was used

(274) MicroRNA-1908-5p contributes to the oncogenic function of the splicing factor SRSF3OncotargetJanuary 31, 2017Kim HR, Shin CH, Lee H, Choi KH, Nam DH, Ohn T, Kim HH.for 24 h and then the firefly reporter vectors were transfected along with renilla expressing vector (E2231; Promega). For validation of miRNA recognition elements, the luciferase constructs containing the binding

(275) Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cellsOncotargetDecember 13, 2016Kim DH, Chang MS, Yoon CJ, Middeldorp JM, Martinez OM, Byeon SJ, Rha SY, Kim SH, Kim YS, Woo JH.using Lipofectamine 2000 (Invitrogen). A Renilla luciferase control reporter vector (0.04 μg, pRL-SV40:E2231; Promega, Madison, WI, USA) was co-transfected to normalize for transfection efficiency. After 72 h,

(276) Direct GR Binding Sites Potentiate Clusters of TF Binding across the Human GenomeCellAugust 25, 2016Christopher M. Vockley, Anthony M. D’Ippolito, Ian C. McDowell, William H. Majoros, Alexias Safi, Lingyun Song, Gregory E. Crawford, Timothy E. ReddyDNA,Recombinant DNA pGL4.24 Lucferase Vector,Promega,E8421 pRL-TK,Promega,E2241 pRL-SV40,Promega,E2231 pSTARR-seq_human,Addgene,Plasmid #71509 Sequence-Based Reagents,Sequence-Based Reagents,Sequence-Based

(277) Macrophage micro-RNA-155 promotes lipopolysaccharide-induced acute lung injury in mice and ratsAmerican journal of physiology. Lung cellular and molecular physiologyAugust 1, 2016Wang W, Liu Z, Su J, Chen WS, Wang XW, Bai SX, Zhang JZ, Yu SQ.the pMIR-REPORTSOCS-1-3=-UTR vector, pRL Renilla luciferase control reporter vector (pRL-SV40 Vector; E2231; Promega, Madison, WI) and miR-155 mimic mmu-miR-155-5p Pre-miR miRNA Precursor (50 nM; PM13058; Thermo

(278) Bigelovii A Protects against Lipopolysaccharide-Induced Acute Lung Injury by Blocking NF-κB and CCAAT/Enhancer-Binding ProteinδPathwaysMediators of InflammationApril 19, 2016Chunguang Yan, Fuqin Guan, Yanfei Shen, Huifang Tang, Dong Yuan, Hongwei Gao, Xu Feng(cat. number: D2206)—was purchased from Beyotime Biotechnology, China. pRL-TK vector (cat. number: E2231) was from Promega Corporation, which was used as an internal control reporter in combination with any

(279) Fine-tuned SRF activity controls asymmetrical neuronal outgrowth: implications for cortical migration, neural tissue lamination and circuit assemblyScientific reportsDecember 7, 2015Scandaglia M, Benito E, Morenilla-Palao C, Fiorenza A, Del Blanco B, Coca Y, Herrera E, Barco A.luciferase reporter plasmids used in this study were pSRE-Luc (Clontech 631911) and pRL-SV40 (Promega E2231), respectively. Luciferase activity was measured at 24 h after transfection, using the Dual Luciferase

(280) Amino-terminal arginylation targets endoplasmic reticulum chaperone BiP for autophagy through p62 bindingNature cell biologyJuly 1, 2015Cha-Molstad H, Sung KS, Hwang J, Kim KA, Yu JE, Yoo YD, Jang JM, Han DH, Molstad M, Kim JG, et al.pRK5-HA-ubiquitin-KO), YFP-CL1 (Addgene, 11950), IgK-IFN β-Luc (Addgene, 14886), and lenillar-luc (Promega, E2231, pRL-SV40 vector). The NHK-GFP plasmid was a gift from Dr. Nobuko Hosokawa (Kyoto University, Japan).

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

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Promega

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pRL-SV40 Vector from Promega

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