pRL-SV40 Vector from Promega

Product Specs
ItempRL-SV40 Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2231
HostMammalian
ResistanceAmpR
PromoterSV40 early enhancer/promoter
Vector NamepRL-SV40
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
3705 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(241) Yap1 safeguards mouse embryonic stem cells from excessive apoptosis during differentiationeLifeDecember 18, 2018LeBlanc L, Lee BK, Yu AC, Kim M, Kambhampati AV, Dupont SM, Seruggia D, Ryu BU, Orkin SH, Kim J.,Sino Biological,Cat#MG50012-UT, Recombinant DNA reagent,pGL3-promoter,Promega,Cat#E1761, Recombinant DNA reagent,pRL-TK,Promega,Cat#E2231, Recombinant DNA reagent,3149 pSFFV-neo Bcl-2 cDNA,AddGene,Cat#8750, Recombinant DNA reagent,"Mus musculus

(242) Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient MiceInvestigative ophthalmology & visual scienceDecember 3, 2018Michael HT, Graff-Cherry C, Chin S, Rauck C, Habtemichael AD, Bunda P, Smith T, Campos MM, Bharti K, Arnheiter H, et al.Fisher, Massachusetts General Hospital, Boston, MA, USA),6 and the normalizer reporter RL vector (#E2231; Promega, Fitchburg, WI, USA) in a 5:1:0.2 ratio using FuGene HD (#E2311; Promega), following the manufacturer's

(243) Peptidyl arginine deiminase 2 (Padi2) is expressed in Sertoli cells in a specific manner and regulated by SOX9 during testicular developmentScientific ReportsDecember 1, 2018Atsumi Tsuji-Hosokawa et al.with pGL4.12 constructs (250 ng; #E6671, Promega) and pRL-SV40 luciferase reporter plasmid (0.5 ng; #E2231, Promega) with expression plasmids (50–75 ng of pSGSox9 and 25–75 ng pcDNA3Foxl2). RNA isolation and

(244) Promoter expression of HERV-K (HML-2) provirus-derived sequences is related to LTR sequence variation and polymorphic transcription factor binding sitesRetrovirologyDecember 1, 2018Meagan Montesion, Zachary H. Williams, Ravi P. Subramanian, Charlotte Kuperwasser, John M. Coffinthe pGL4 vector alongside a pRL-SV40 internal control Renilla luciferase vector (Promega, Cat. No. E2231) at a 30:1 ratio using Opti-MEM reduced serum media (Gibco, Cat. No. 31985-070) and Lipofectamine 2000

(245) BC094916 suppressed SP 2/0 xenograft tumor by down-regulating Creb1 and Bcl2 transcriptionCancer Cell InternationalDecember 1, 2018Ruonan Xu, Ying Fang, Chunmei Hou, Bing Zhai, Zhenyu Jiang, Ning Ma, Liang Wang, Gencheng Han, Renxi Wang(General Biosystems, Anhui, China), and 0.05 μg Renilla luciferase reporter vector pRL-SV-40 vector (cat# E2231, Promega Corp.) were co-transduced into 4 × 105 SP 2/0 or 293T cells in 12-well plate by using 6 μL

(246) Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T CellsCell reportsNovember 20, 2018Kim C, Hu B, Jadhav RR, Jin J, Zhang H, Cavanagh MM, Akondy RS, Ahmed R, Weyand CM, Goronzy JJ.DNA psPAX2,Addgene,Cat#12260 pMD2.G,Addgene,Cat#12259 AP-1 luciferase reporter plasmid,Addgene,Cat#40342 pRL-SV40 renilla luciferase reporter,Promega,Cat# E2231 ,, Software and Algorithms,Software and Algorithms,Software and Algorithms FlowJo,TreeStar,RRID:SCR_008520 Prism,GraphPad

(247) Diminished Canonical β‐Catenin Signaling During Osteoblast Differentiation Contributes to Osteopenia in ProgeriaJournal of Bone and Mineral ResearchNovember 1, 2018Ji Young Choi, Jim K Lai, Zheng‐Mei Xiong, Margaret Ren, Megan C Moorer, Joseph P Stains, Kan Caobiotechnology) flash luciferase reporter vector and internal control Renilla plasmid, pRL-SV40 vector (#E2231; Promega) using Lipofectamine 2000 transfection reagent (#11668; Invitrogen) in Opti-MEM reduced serum

(248) Widespread Selection for Oncogenic Mutant Allele Imbalance in CancerCancer CellNovember 1, 2018Craig M. Bielski, Mark T.A. Donoghue, Mayur Gadiya, Aphrothiti J. Hanrahan, Helen H. Won, Matthew T. Chang, Philip Jonsson, Alexander V. Penson, Alexander Gorelick, Christopher Harris, et al.Proteins" pEGPF-N1-MEK1,Addgene,Cat#14746 3X-ERE-TATA-luciferase reporter,Addgene,Cat#11354 pRL-TK Renilla luciferase plasmid,Promega,Cat#E2231 HA-tagged WT ERα,This study,N/A pcDNA3.1 (EV),Thermo Fisher,Cat#V79020 HA-tagged ERα Y537S,This study,N/A HA-tagged

(249) The Mitochondrial-Encoded Peptide MOTS-c Translocates to the Nucleus to Regulate Nuclear Gene Expression in Response to Metabolic StressCell metabolismSeptember 4, 2018Kim KH, Son JM, Benayoun BA, Lee C.Script,N/A pcDNA3.1,Thermo Fisher Scientific,Cat# V79520 Renilla luciferase,Promega Corporation,Cat#E2231 pGL4.37[luc2P/ARE/Hygm]),Promega Corporation,Cat#E3641 Software and Algorithms,, kallisto 0.43.0,"Bray

(250) MiR-486 promotes proliferation and suppresses apoptosis in myeloid cells by targeting Cebpa in vitroCancer medicineSeptember 1, 2018Jiang J, Gao Q, Gong Y, Huang L, Lin H, Zhou X, Liang X, Guo W.804005; Shanghai GenePharma Co., Ltd, Suzhou, China), and reporter plasmid pRL‐TK (Catalog number: E2231; Promega) with renilla luciferase using Lipofectamine 2000 reagent (Catalog number: 11668019; Invitrogen)

(251) Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear ImportCellAugust 23, 2018Rodriguez-Bravo V, Pippa R, Song WM, Carceles-Cordon M, Dominguez-Andres A, Fujiwara N, Woo J, Koh AP, Ertel A, Lokareddy RK, et al.POM121 Promoter GBE3 mutant,This paper,N/A pRL- Renilla Luciferase Control Reporter Vector,Promega,E2231 pCMV-GATA2,"Vidal etal., 2015",N/A Software and Algorithms,Software and Algorithms,Software and Algorithms Prism,GraphPad,https://www.graphpad.com/scientific-software/prism/ Living

(252) MNX1 reduces sensitivity to anoikis by activating TrkB in human glioma cellsMolecular Medicine ReportsJuly 27, 2018Lai Jiang, Shaojun Chen, Donggang Zhao, Jun Yan, Jiemin Chen, Chunlin Yang, Gang Zhengwith 800 ng target plasmid and 15 ng control Renilla reniformis luciferase plasmid (pRL-TK; cat. no. E2231; Promega Corporation) using 1 µl Lipofectamine® (Invitrogen; Thermo Fisher Scientific, Inc.). After

(253) DNA-mediated dimerization on a compact sequence signature controls enhancer engagement and regulation by FOXA1Nucleic Acids ResearchJune 20, 2018Xuecong Wang, Yogesh Srivastava, Aleksander Jankowski, Vikas Malik, Yuanjie Wei, Ricardo CH del Rosario, Vlad Cojocaru, Shyam Prabhakar, Ralf Jauchgrown for 24 h to reach 80% confluency. 200 ng luciferase reporter plasmids, 1 ng pRL-SV40 Renilla (E2231, Promega) and 50 ng pcDNA3-FOXA1 were mixed with 0.8 μl of X-tremeGENE 9 DNA Transfection Reagent (Roche)

(254) Intein-mediated backbone cyclization of entolimod confers enhanced radioprotective activity in mouse modelsPeerJJune 18, 2018Bingyu Ye, Wenlong Shen, Minglei Shi, Yan Zhang, Cunshuan Xu, Zhihu Zhaoconfluence were transfected with plasmids pNF-κB-Luc (#219078; Agilent, Santa Clara, CA, USA) and pRL-SV40 (#E2231; Promega, Madison, WI, USA) using Lipofectamine™ 2000 (#11668019; ThermoFisher Scientific, Waltham,

(255) Human-Specific NOTCH2NL Genes Affect Notch Signaling and Cortical NeurogenesisCellMay 31, 2018Fiddes IT, Lodewijk GA, Mooring M, Bosworth CM, Ewing AD, Mantalas GL, Novak AM, van den Bout A, Bishara A, Rosenkrantz JL, et al.study,na pCIG2a-NOTCH2-Myc,This study,na pCIG2a-PDGFR-Myc,This study,na pCIG2a-EGFR-Myc,This study,na pRL-CMV,Promega,E2231 pGL3-UAS,Marc Vooijs,na pcDNA5-NOTCH1-GAL4-TAD-N1,Marc Vooijs,na pcDNA5-NOTCH2-GAL4-TAD-N1,Marc Vooijs,na pLVX-NOTCH3-GAL4GV16,Marc

(256) Deletion of BMAL1 in Smooth Muscle Cells Protects Mice From Abdominal Aortic AneurysmsArteriosclerosis, Thrombosis, and Vascular BiologyMay 1, 2018Jenny Lutshumba, Shu Liu, Yu Zhong, Tianfei Hou, Alan Daugherty, Hong Lu, Zhenheng Guo, Ming C. Gonga pGl3-Timp4-promoter firefly luciferase construct and a pRL-SV40 renilla luciferase control vector (Cat No. E2231; Promega) using Lipofectamine-Plus reagent (Cat No. 15338100; Invitrogen). The Timp4 luciferase promoter

(257) eIF4A alleviates the translational repression mediated by classical secondary structures more than by G-quadruplexesNucleic Acids ResearchApril 6, 2018Joseph A Waldron, Farheen Raza, John Le Quesnesequence cloned upstream of the Fluc ORF) and 50ng pRL Renilla Luciferase Control SV40 plasmid (Promega E2231), using 1.5 μl Lipofectamine 2000 transfection reagent (ThermoFisher 11668019) in duplicate. Eight hours

(258) IRF4 haploinsufficiency in a family with Whipple's diseaseeLifeMarch 14, 2018Guérin A, Kerner G, Marr N, Markle JG, Fenollar F, Wong N, Boughorbel S, Avery DT, Ma CS, Bougarn S, et al.with the (ISRE)3 reporter plasmid (100 ng/well on a 96-well plate), the pRL-SV40 vector (Promega # E2231, 40 ng/well) and a IRF4 WT or mutant pcDNA 3.1D/V5-His-TOPO plasmid (Invitrogen #K4900-01, 25 ng/well

(259) Network of MicroRNAs Mediate Translational Repression of Bone Morphogenetic Protein Receptor‐2: Involvement in HIV‐Associated Pulmonary Vascular RemodelingJournal of the American Heart AssociationMarch 6, 2018Mahendran Chinnappan, Aradhana Mohan, Stuti Agarwal, Pranjali Dalvi, Navneet K. DhillonpRL‐TK (renilla luciferase reporter mRNA) containing renilla luciferase was purchased from Promega (Cat#E2231). Both the firefly and renilla luciferases were under T7 promoter in their respective vectors to enable

(260) Network of MicroRNAs Mediate Translational Repression of Bone Morphogenetic Protein Receptor‐2: Involvement in HIV‐Associated Pulmonary Vascular RemodelingJ Am Heart AssocFebruary 25, 2018Mahendran Chinnappan, Aradhana Mohan, Stuti Agarwal, Pranjali Dalvi, Navneet K. DhillonpRL‐TK (renilla luciferase reporter mRNA) containing renilla luciferase was purchased from Promega (Cat#E2231). Both the firefly and renilla luciferases were under T7 promoter in their respective vectors to enable

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pRL-SV40 Vector

pRL-SV40 Vector from Promega

Description

Specifications/Features

Promega

Citations (323)

Reviews

pRL-SV40 Vector from Promega

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