psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(161) Identification of Tumor Suppressive Genes Regulated by miR-31-5p and miR-31-3p in Head and Neck Squamous Cell CarcinomaInternational Journal of Molecular SciencesJune 8, 2021Sachi Oshima et al.inserted between the XhoI-PmeI restriction sites in the 3′-UTR of the hRluc gene in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Alternatively, we used sequences that were missing the miR-31-3p target

(162) FKBP39 controls nutrient dependent Nprl3 expression and TORC1 activity in DrosophilaCell death & diseaseJune 2, 2021Zhou Y, Guo J, Wang X, Cheng Y, Guan J, Barman P, Sun MA, Fu Y, Wei W, Feng C, et al.mutated 5′UTRs of nprl3 were amplified using specific primers and inserted into psiCKECK2 (Promega, #C8021) to construct a series of Renilla luciferase reporter plasmids. The nprl3 and FKBP39 coding regions

(163) miR-188-5p promotes oxaliplatin resistance by targeting RASA1 in colon cancer cellsOncology lettersJune 1, 2021Zhu X, Luo X, Song Z, Jiang S, Long X, Gao X, Xie X, Zheng L, Wang H.(UTR) to the psiECHECK‑2 vector containing a luciferase reporter gene (Promega Corporation; cat. no. C8021). SW480 and SW480/OXA cells (5x105/well) were seeded into 6‑well plates and co‑transfected with RASA1

(164) Abiraterone induces SLCO1B3 expression in prostate cancer via microRNA-579-3pScientific ReportsMay 24, 2021Roberto H. Barbier, Edel M. McCrea, Kristi Y. Lee, Jonathan D. Strope, Emily N. Risdon, Douglas K. Price, Cindy H. Chau, William D. Figga psiCHECK-2 vector, which contains both renilla and firefly luciferase reporter genes (Promega, CAT#: C8021). The constructed plasmid was sequenced to verify successful cloning. 22Rv1 cells were seeded in a 96-well

(165) Endogenous p53 expression in human and mouse is not regulated by its 3'UTReLifeMay 6, 2021Mitschka S, Mayr C.paper,,see Materials and methods Supplementary file 1 Transfected construct (synthetic),psiCHECK-2,Promega,C8021, Transfected construct (Homo sapiens),psiCHECK-2_TP53-3UTR,This paper,,see Materials and methods Supplementary

(166) MicroRNA governs bistable cell differentiation and lineage segregation via a noncanonical feedbackMolecular Systems BiologyApril 23, 2021Chung‐Jung Li, Ee Shan Liau, Yi‐Han Lee, Yang‐Zhe Huang, Ziyi Liu, Andrew Willems, Victoria Garside, Edwina McGlinn, Jun‐An Chen, Tian HongThermo Fisher Scientific). The purified PCR products were then inserted into the psiCHECKTM‐2 vector (C8021; Promega) at XhoI and NotI restriction sites by using T4 DNA ligase (M0202S; NEB). The mutated versions

(167) MicroRNA-22 contributes to dexamethasone-induced osteoblast differentiation inhibition and dysfunction through targeting caveolin-3 expression in osteoblastic cellsExperimental and therapeutic medicineApril 1, 2021Li P, Mao W, Zhang S, Zhang L, Chen Z, Lu Z.CAV3 were synthesized in vitro and cloned into the psiCHECK2 luciferase reporter plasmid (cat. no. C8021; Promega Corporation). Osteoblastic MC3T3-E1 cells were co-transfected with the psiCHECK-2 plasmid containing

(168) MicroRNAs mediate precise control of spinal interneuron populations to exert delicate sensory-to-motor outputseLifeMarch 31, 2021Chang SH, Su YC, Chang M, Chen JA.Laboratory Cat# JAX:003724, RRID:IMSR_JAX:003724 Recombinant DNA psiCHECK-2 vector Promega Cat# C8021, RRID:Addgene_106979 Recombinant DNA psiCHECK-2-Satb2-3’UTR_WT This paper N/A Recombinant DNA psiCHECK-2-Satb2-3’UTR_Mut This

(169) miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancerOpen Life SciencesMarch 23, 2021Zhenfen Wang, Qing Liu, Ping Huang, Guohao Caiinserted into the downstream of the stop codon of psiCHECK2 dualluciferase reporter plasmids (Cat. no C8021; Promega, Madison, WI, USA) and named as PAX3-Wt or PAX3-Mut (Geneseed, Guangzhou, China). Then the

(170) MiR-200c-3p Contrasts PD-L1 Induction by Combinatorial Therapies and Slows Proliferation of Epithelial Ovarian Cancer through Downregulation of β-Catenin and c-MycCellsMarch 1, 2021Anastasiadou E, Messina E, Sanavia T, Mundo L, Farinella F, Lazzi S, Megiorni F, Ceccarelli S, Pontecorvi P, Marampon F, et al.previously established reporter system of PD-L1 30 UTR psiCHECK™-2 Vector (Promega Corporation, Madison, WI, USA; Cat. n.C8021) was transiently transfected into SKOV3 pCMV Vector and SKOV3 pCMVmiR-200c carrying SKOV3 cell lines

(171) CircTM7SF3 contributes to oxidized low-density lipoprotein-induced apoptosis, inflammation and oxidative stress through targeting miR-206/ASPH axis in atherosclerosis cell model in vitroBMC cardiovascular disordersFebruary 2, 2021Wang X, Bai M.in circTM7SF3 were amplified and cloned into psiCHECK-2 plasmid (Promega, Madison, WI, USA; Cat. no. C8021) to generate circTM7SF3-wild type (WT). The wild-type sequence of ASPH was also cloned into psiCHECK-2

(172) MiR-CLIP reveals iso-miR selective regulation in the miR-124 targetomeNucleic Acids ResearchJanuary 11, 2021Yuluan Wang, Charlotte Soneson, Anna L Malinowska, Artur Laski, Souvik Ghosh, Alexander Kanitz, Luca F R Gebert, Mark D Robinson, Jonathan Hallplasmids (Supplementary Table S2) were generated by DNA synthesis, then cloned into psiCHECK2 vector (no. C8021, Promega, Dübendorf). HEK293T cells were seeded into 96-well plates. Cells were transfected with indicated

(173) EHHADH contributes to cisplatin resistance through regulation by tumor-suppressive microRNAs in bladder cancerBMC cancerJanuary 11, 2021Okamura S, Yoshino H, Kuroshima K, Tsuruda M, Osako Y, Sakaguchi T, Yonemori M, Yamada Y, Tatarano S, Nakagawa M, et al.between the XhoI and PmeI restriction sites in the 3′-UTR of the hRluc gene in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). CDDP-R-BOY and CDDP-R-T24 cells were transfected with 50 ng of vector and

(174) PUM1 Is Overexpressed in Colon Cancer Cells With Acquired Resistance to CetuximabFrontiers in cell and developmental biologyJanuary 1, 2021Liu Q, Xin C, Chen Y, Yang J, Chen Y, Zhang W, Ye L.al., 2015). Dual-Luciferase Assays We constructed the 3′ UTR of DDX5 into the plasmid psiCHECK2 (C8021, Promega) carrying double fluorescent reporter gene, then we transfected an empty plasmid or the constructed

(175) FAIM Is Regulated by MiR-206, MiR-1-3p and MiR-133bFrontiers in Cell and Developmental BiologyDecember 23, 2020Elena Coccia, Marc Masanas, Joaquín López-Soriano, Miguel F. Segura, Joan X. Comella, M. José Pérez-GarcíaWaltham, MA, United States) and cloned into the psiCheckTM -2 dual luciferase reporter vector (Promega, C8021). For luciferase assays, HEK293T (Cat# CRL-3216, RRID:CVCL_0063) cells were co-transfected with 50 ng

(176) Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer CellsEvidence-Based Complementary and Alternative MedicineNovember 29, 2020Congyuan Zhu, Jianping Li, Yuming Hua, Jingli Wang, Ke Wang, Jingqiu SunmiR-214-3p. Reporter gene plasmids containing wild-type and mutant SCT 3′UTR, psiCHECK-2 plasmids (Promega, C8021, USA), miR-214-3p mimic, and their controls were transfected into MCF-7 and MDA-MB-231 cells for 48

(177) Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feedingLife Science AllianceOctober 1, 2020Mosharrof Mondal, Judith K Brown, Alex Flyntfor double-stranded and ssRNA synthesis. 231-nt luciferase gene sequence from psiCHECK-2 (Cat. no. C8021; Promega) vector was cloned into the pGEMT-easy vector. ssRNA and dsRNA from the luciferase sequence

(178) MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cellsBiological researchSeptember 29, 2020Cao D, Zhu H, Zhao Q, Huang J, Zhou C, He J, Liang Y.and then cloned into the downstream of the Renilla luciferase (hRluc) gene in the psiCHECK™-2 Vector (C8021; Promega, Madison, WI, USA). The mutant vector containing four separate mutated bases on the predicted

(179) Design of an Interferon-Resistant Oncolytic HSV-1 Incorporating Redundant Safety Modalities for Improved TolerabilityMolecular Therapy - OncolyticsSeptember 25, 2020Edward M. Kennedy, Terry Farkaly, Peter Grzesik, Jennifer Lee, Agnieszka Denslow, Jacqueline Hewett, Jeffrey Bryant, Prajna Behara, Caitlin Goshert, Daniel Wambua, et al.in a CO2 incubator. mirVana miRNA mimics were co-transfected in triplicate with psiCHECK-2 (Promega, C8021) vectors using Lipofectamine 2000 (Invitrogen, Thermo Fisher, 11668-019) according to manufacturer’s

(180) miR-301a Suppression within Fibroblasts Limits the Progression of Fibrosis through the TSC1/mTOR PathwayMolecular therapy. Nucleic acidsSeptember 4, 2020Wang J, Li X, Zhong M, Wang Y, Zou L, Wang M, Gong X, Wang X, Zhou C, Ma X, et al.including two predicted miRNA recognition elements, was synthesized and subcloned into the psiCHECK-2 (C8021, Promega, USA) dual luciferase reporter vector. HEK293T cells were transfected with the psiCHECK-2 vector

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