psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(181) EHHADH contributes to cisplatin resistance through regulation by tumor-suppressive microRNAs in bladder cancerBMC cancerJanuary 11, 2021Okamura S, Yoshino H, Kuroshima K, Tsuruda M, Osako Y, Sakaguchi T, Yonemori M, Yamada Y, Tatarano S, Nakagawa M, et al.between the XhoI and PmeI restriction sites in the 3′-UTR of the hRluc gene in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). CDDP-R-BOY and CDDP-R-T24 cells were transfected with 50 ng of vector and

(182) MiR-CLIP reveals iso-miR selective regulation in the miR-124 targetomeNucleic Acids ResearchJanuary 11, 2021Yuluan Wang, Charlotte Soneson, Anna L Malinowska, Artur Laski, Souvik Ghosh, Alexander Kanitz, Luca F R Gebert, Mark D Robinson, Jonathan Hallplasmids (Supplementary Table S2) were generated by DNA synthesis, then cloned into psiCHECK2 vector (no. C8021, Promega, Dübendorf). HEK293T cells were seeded into 96-well plates. Cells were transfected with indicated

(183) PUM1 Is Overexpressed in Colon Cancer Cells With Acquired Resistance to CetuximabFrontiers in cell and developmental biologyJanuary 1, 2021Liu Q, Xin C, Chen Y, Yang J, Chen Y, Zhang W, Ye L.al., 2015). Dual-Luciferase Assays We constructed the 3′ UTR of DDX5 into the plasmid psiCHECK2 (C8021, Promega) carrying double fluorescent reporter gene, then we transfected an empty plasmid or the constructed

(184) FAIM Is Regulated by MiR-206, MiR-1-3p and MiR-133bFrontiers in Cell and Developmental BiologyDecember 23, 2020Elena Coccia, Marc Masanas, Joaquín López-Soriano, Miguel F. Segura, Joan X. Comella, M. José Pérez-GarcíaWaltham, MA, United States) and cloned into the psiCheckTM -2 dual luciferase reporter vector (Promega, C8021). For luciferase assays, HEK293T (Cat# CRL-3216, RRID:CVCL_0063) cells were co-transfected with 50 ng

(185) Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer CellsEvidence-Based Complementary and Alternative MedicineNovember 29, 2020Congyuan Zhu, Jianping Li, Yuming Hua, Jingli Wang, Ke Wang, Jingqiu SunmiR-214-3p. Reporter gene plasmids containing wild-type and mutant SCT 3′UTR, psiCHECK-2 plasmids (Promega, C8021, USA), miR-214-3p mimic, and their controls were transfected into MCF-7 and MDA-MB-231 cells for 48

(186) Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feedingLife Science AllianceOctober 1, 2020Mosharrof Mondal, Judith K Brown, Alex Flyntfor double-stranded and ssRNA synthesis. 231-nt luciferase gene sequence from psiCHECK-2 (Cat. no. C8021; Promega) vector was cloned into the pGEMT-easy vector. ssRNA and dsRNA from the luciferase sequence

(187) MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cellsBiological researchSeptember 29, 2020Cao D, Zhu H, Zhao Q, Huang J, Zhou C, He J, Liang Y.and then cloned into the downstream of the Renilla luciferase (hRluc) gene in the psiCHECK™-2 Vector (C8021; Promega, Madison, WI, USA). The mutant vector containing four separate mutated bases on the predicted

(188) Design of an Interferon-Resistant Oncolytic HSV-1 Incorporating Redundant Safety Modalities for Improved TolerabilityMolecular Therapy - OncolyticsSeptember 25, 2020Edward M. Kennedy, Terry Farkaly, Peter Grzesik, Jennifer Lee, Agnieszka Denslow, Jacqueline Hewett, Jeffrey Bryant, Prajna Behara, Caitlin Goshert, Daniel Wambua, et al.in a CO2 incubator. mirVana miRNA mimics were co-transfected in triplicate with psiCHECK-2 (Promega, C8021) vectors using Lipofectamine 2000 (Invitrogen, Thermo Fisher, 11668-019) according to manufacturer’s

(189) miR-301a Suppression within Fibroblasts Limits the Progression of Fibrosis through the TSC1/mTOR PathwayMolecular therapy. Nucleic acidsSeptember 4, 2020Wang J, Li X, Zhong M, Wang Y, Zou L, Wang M, Gong X, Wang X, Zhou C, Ma X, et al.including two predicted miRNA recognition elements, was synthesized and subcloned into the psiCHECK-2 (C8021, Promega, USA) dual luciferase reporter vector. HEK293T cells were transfected with the psiCHECK-2 vector

(190) 2â€²-O-Methyl at 20-mer Guide Strand 3â€² Termini May Negatively Affect Target Silencing Activity of Fully Chemically Modified siRNAMolecular Therapy - Nucleic AcidsSeptember 4, 2020Sarah M. Davis, Jacquelyn Sousa, Lorenc Vangjeli, Matthew R. Hassler, Dimas Echeverria, Emily Knox, Anton A. Turanov, Julia F. Alterman, Anastasia KhvorovasFLT1-i13 and sFLT1-e15a target mRNAs, were cloned into a psiCheck-2 vector (Promega, Madison, WI, USA; C8021), according to the manufacturer’s protocol. HeLa cells were plated on a 10-cm dish and transfected with

(191) Silencing of Long Noncoding RNA LINC00324 Interacts with MicroRNA-3200-5p to Attenuate the Tumorigenesis of Gastric Cancer via Regulating BCAT1Gastroenterology Research and PracticeAugust 13, 2020Shuang Wang, Yuanyuan Cheng, Pingping Yang, Guang Qinor MUT miR3200-5p-binding sites were generated and subcloned into the psiCHECK-2 vector (catalog # C8021, Promega, Madison, WI, USA). The vectors were then cotransfected with mimics NC, miR-3200-5p mimics,

(192) High-Resolution In Vivo Identification of miRNA Targets by Halo-Enhanced Ago2 Pull-DownMolecular CellJuly 1, 2020Xiaoyi Li et al.31467 pTRETightBI-RY-4,"Mukherji et al., 2011",Addgene: 31465 rtTA-N144,"Richner et al., 2015",Addgene: 66810 psiCheck2,Promega,C8021 psiCheck2-Pten-miR-29-WT,This paper,N/A psiCheck2-Pten-miR-29-MUT,This paper,N/A psiCheck2-Adrb2-let-7-WT,This

(193) MicroRNA‑93 promotes angiogenesis and attenuates remodeling via inactivation of the Hippo/Yap pathway by targeting Lats2 after myocardial infarctionωMolecular medicine reportsJuly 1, 2020Ma C, Peng P, Zhou Y, Liu T, Wang L, Lu C.mutant sequence (Lats2-3′-UTR-MUT) were cloned into psiCHECK-2 vectors (Promega Corporation; cat. no. C8021). HUVECs were co-transfected with Lats2-3′-UTR-WT (1 µg) or Lats2-3′-UTR-MUT (1 µg) reporter, and miR-93

(194) Exosomal miR-200c-3p negatively regulates the migraion and invasion of lipopolysaccharide (LPS)-stimulated colorectal cancer (CRC)BMC molecular and cell biologyJune 29, 2020Jiang Y, Ji X, Liu K, Shi Y, Wang C, Li Y, Zhang T, He Y, Xiang M, Zhao R.luciferase reporter assay The 3′-UTR regions of human Zeb-1 and Zeb-2 genes were cloned into psiCHECK2 plasmid (Promega, C8021) for luciferase reporter assay. The sequences of cloning primers were reported in previous publications

(195) Propofol‑induced miR‑125a‑5p inhibits the proliferation and metastasis of ovarian cancer by suppressing LIN28BMolecular Medicine ReportsJune 11, 2020Juan Zeng, Yu‑Kun Li, Fei‑Fei Quan, Xin Zeng, Chang‑Ye Chen, Tian Zeng, Juan Zou, Wen‑Juan Tonginserted into the psiCheck2 Luciferase vector (Promega Corporation). The psi-CHECK2 vector (cat. no. C8021) was purchased from Promega Corporation. The miR-125a-5p mimics and negative control (NC) mimics, and

(196) A Novel Mechanism of bta-miR-210 in Bovine Early Intramuscular AdipogenesisGenesMay 29, 2020Ren L, Li Q, Hu X, Yang Q, Du M, Xing Y, Wang Y, Li J, Zhang L.recombined at the 3′ end of the SV40 promoter-driven Renilla luciferase gene in the psiCHECK-2 vector (C8021, Promega, Madison, WI, USA). The other luciferase gene in psiCHECK-2, the firefly luciferase gene, was

(197) Structure-Mediated RNA Decay by UPF1 and G3BP1Molecular cellApril 2, 2020Fischer JW, Busa VF, Shao Y, Leung AKL.Lentivirus expression system,Addgene,Plasmid #8453 psiCHECK2 Luciferase expression system,Promega,C8021 Flp-In T-Rex Core Kit,"Andrew Holland,Thermo",K650001 pEGFP-C1,Clontech,GenBank #U55763 Software

(198) miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitaryJournal of EndocrinologyMarch 1, 2020Xin Li, Hongjiao Li, Di Zhang, Guojin Xu, Jinglin Zhang, Sheng Cui4370; Cell Signaling Technology) were used. Dual-luciferase reporter assay The psiCHECK™-2 vector (C8021; Promega) was used for constructing the dual-luciferase reporter genes. Wild-type and mutant 3′UTR of

(199) HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5pCell Death & DiseaseMarch 1, 2020Yufan Zhou, Yun Huang, Kuan Hu, Zeyu Zhang, Jiajin Yang, Zhiming WanglncRNA RAET1K or LDHA-3′UTR was amplified and cloned downstream of Renilla in the psiCHECK2 vector (C8021, Promega, Madison, WI, USA), and the construct was named wt-RAET1K or wt-LDHA. To generate the lncRNA

(200) hsa-miR-9-3p and hsa-miR-9-5p as Post-Transcriptional Modulators of DNA Topoisomerase IIαin Human Leukemia K562 Cells with Acquired Resistance to EtoposideMolecular PharmacologyMarch 1, 2020Evan E. Kania, Jessika Carvajal-Moreno, Victor A. Hernandez, Anthony English, Jonathan L. Papa, Nicholas Shkolnikov, Hatice Gulcin Ozer, Ayse Selen Yilmaz, Jack C. Yalowich, Terry S. EltonConstructs. The psiCHECK-2 dual luciferase vector was purchased from Promega Corporation (cat. no. C8021; Madison, WI) and linearized by XhoI digestion (cat. no. R0146S; New England Biolabs, Ipswich, MA) according

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