psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) miR181a/b-1 controls osteocyte metabolism and mechanical properties independently of bone morphologyBoneOctober 1, 2023Jihee Yoon, Serra Kaya, Gen Matsumae, Neha Dole, Tamara Alliston72 °C for 1 min, and a final extension at 72 °C for 3 min. Amplicon and Psicheck2 vector (Promega, C8021) were digested at Not1 and Zho1 restriction sites and ligated using T4 DNA Ligase (New England Biolabs,

(82) Asymmetric trichotomous partitioning overcomes dataset limitations in building machine learning models for predicting siRNA efficacyMolecular Therapy - Nucleic AcidsSeptember 12, 2023Kathryn R. Monopoli, Dmitry Korkin, Anastasia Khvorovaconstructed with fusions of the 20-nt targeting region sequences inserted into a psiCHECK-2 vector (Promega, C8021).

(83) Exosomal miRNA-223-3p derived from tumor associated macrophages promotes pulmonary metastasis of breast cancer 4T1 cellsTranslational OncologySeptember 1, 2023Ziyuan Wang, Chen Zhang, Jian Guo, Wei Wang, Qin Si, Chong Chen, Yunping Luo, Zhaojun Duansequence predicted to be bound by miR-223-3p in the 3’UTR of Cbx5 was cloned into psiCHECK-2 vector (C8021, Promega), locating at the 3’UTR of synthetic renilla luciferase gene. A mutant vector of which the

(84) Minichromosome maintenance proteins in lung adenocarcinoma: Clinical significance and therapeutic targetsFEBS open bioSeptember 1, 2023Tanigawa K, Tomioka Y, Misono S, Asai S, Kikkawa N, Hagihara Y, Suetsugu T, Inoue H, Mizuno K, Seki N.(UTRs) of MCM2 and the deletion‐type sequences lacking them were cloned into the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). The procedures for transfection and dual‐luciferase reporter assays were

(85) Comparative analysis of the myoglobin gene in whales and humans reveals evolutionary changes in regulatory elements and expression levelsPLOS ONEAugust 29, 2023Charles Sackerson, Vivian Garcia, Nicole Medina, Jessica Maldonado, John Daly, Rachel CartwrightpSV-Rluc renilla luciferase internal standard vector was derived from psiCHECK-2 (Promega, catalog # C8021) by digestion with EagI to remove the firefly luciferase gene, leaving the renilla luciferase gene driven

(86) The oncogene Musashi1 encodes novel miRNAs in breast cancerSci RepAugust 22, 2023Liana Lachinani, Mahboobeh Forouzanfar, Kianoush Dormiani, Bahram Mohammad Soltani, Kamran Dolatshahi, Sayyed Mohammadreza Hakimian, Sadat Dokanehiifard, Mohammad Hossein Nasr-EsfahanipBud-EGFP vector at SalI and XbaI sites (Table 1B). For dual luciferase assay, psiCHECK2 (Cat. No. C8021; Promega) reporter vectors containing wild-type or mutant 3′UTR regions of the target genes were constructed.

(87) Deletion of miR-146a enhances therapeutic protein restoration in model of dystrophin exon skippingbioRxivAugust 12, 2023Nikki M. McCormack, Kelsey A. Calabrese, Christina M. Sun, Christopher B. Tully, Christopher R. Heier, Alyson A. Fiorillowas gel purified, digested with XhoI and NotI, and ligated into the pSiCheck2 plasmid (Promega Cat #C8021) where renilla luciferase is upstream of the 3’UTR and firefly luciferase is driven by a separate promoter

(88) Loss of Paip1 causes translation reduction and induces apoptotic cell death through ISR activation and Xrp1Cell Death DiscovAugust 5, 2023Maoguang Xue, Fei Cong, Wanling Zheng, Ruoqing Xu, Xiaoyu Liu, Hongcun Bao, Ying Ying Sung, Yongmei Xi, Feng He, Jun Ma, et al.AGTAATCCATGTACAAAATAACCAAC, Xrp1-5’UTRR -CAATATTATATCTCTGGGAT) and subcloned into the psiCHECK2 vector (Promega, C8021) upstream of Renilla-luciferase. Briefly, the psiCHECK2 vector was first linearized by Nhe I (NEB, R3131V).

(89) The Molecular Pathogenesis of Tumor-Suppressive miR-486-5p and miR-486-3p Target Genes: GINS4 Facilitates Aggressiveness in Lung AdenocarcinomaCellsJuly 18, 2023Tomioka Y, Suetsugu T, Seki N, Tanigawa K, Hagihara Y, Shinmura M, Asai S, Kikkawa N, Inoue H, Mizuno K.Dual-Luciferase Reporter Assays The following two sequences were cloned into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA): the wild-type sequence of the 3′-untranslated regions (UTRs) of GINS4 and

(90) Stimulation of ectopically expressed muscarinic receptors induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cellsProceedings of the National Academy of Sciences of the United States of AmericaJune 20, 2023Nguyen TTT, Lu W, Zhu WS, Ansel KM, Liang HE, Weiss A.mRNA. Murine 3′UTR of IL-2 mRNA or IFN-γ mRNA was cloned into the dual-luciferase vector (psicheck2) (Promega, C8021) using XhoI and NotI sites. The Psicheck2 plasmid containing 3′UTR of IL-2 or IFN-γ mRNA was transduced

(91) Selective binding of retrotransposons by ZFP352 facilitates the timely dissolution of totipotency networkNature CommunicationsJune 20, 2023Zhengyi Li, Haiyan Xu, Jiaqun Li, Xiao Xu, Junjiao Wang, Danya Wu, Jiateng Zhang, Juan Liu, Ziwei Xue, Guankai Zhan, et al.pGL4.23 luciferase reporter plasmid containing the SV40 mini-promoter, or the psi-CHECK-2 (Promega, C8021) luciferase reporter plasmid. Luciferase reporter plasmids were transfected into HEK293T cells together

(92) Synthetic miR-21 decoy circularized by tRNA splicing mechanism inhibited tumorigenesis in glioblastoma in vitro and in vivo modelsMolecular therapy. Nucleic acidsJune 13, 2023Bayat H, Pourgholami MH, Rahmani S, Pournajaf S, Mowla SJ.of the RNA decoys, TIMP3 3′-UTR was amplified and cloned into a psiCHECKTM-2 vector (Promega; Cat. #C8021) between XhoI (Thermo Fisher Scientific; Cat. #ER0691) and NotI (named pTIMP3-UTR).24 To construct the

(93) The ribosome-associated protein RACK1 represses Kir4.1 translation in astrocytes and influences neuronal activityCell reportsMay 30, 2023Oudart M, Avila-Gutierrez K, Moch C, Dossi E, Milior G, Boulay AC, Gaudey M, Moulard J, Lombard B, Loew D, et al.control cassette in frame with both the GFP and mCherry coding sequences. The psiCHECK-2 vector (Promega, C8021) was used to build the dual luciferase reporters with Kcnj10 UTRs. The UTR sequences of mouse Kcnj10

(94) LncRNA FALEC increases the proliferation, migration and drug resistance of cholangiocarcinoma through competitive regulation of miR-20a-5p/SHOC2 axisAging (Albany NY)May 15, 2023Haiming Du, Senlin Hou, Lichao Zhang, Chao Liu, Tingting Yu, Wei Zhangcells. Dual-luciferase reporter assay We obtained the cDNA clone of FALEC from NCBI (Gene ID: 100874054). The plasmid (C8021, psiCHECK™-2 Vectors, Promega) containing the full length wild-type (WT) or mutant (Mut) FALEC miRNA

(95) miR-34c-3p Regulates Protein Kinase A Activity Independent of cAMP by Dicing prkar2b Transcripts in Theileria annulata-Infected LeukocytesmSphereApril 20, 2023Malak Haidar, Shahin Tajeri, Laurence Momeux, Tobias Mourier, Fathia Ben-Rached, Sara Mfarrej, Zineb Rchiad, Arnab Pain, Gordon Langsleythe identified canonical miR-34c-3p seed sequence, was subcloned into the psiCHECK-2 vector (Promega; C8021) and transfected into attenuated macrophages, together with the miR-34c-3p mimic, and the Renilla luciferase

(96) RNAi-mediated rheostat for dynamic control of AAV-delivered transgenesNature communicationsApril 8, 2023Subramanian M, McIninch J, Zlatev I, Schlegel MK, Kaittanis C, Nguyen T, Agarwal S, Racie T, Alvarado MA, Wassarman K, et al.containing a fully complementary (23-mer) or partial siRNA target sites into the psiCHECK2 vector (Promega C8021) between Xho1 and Not1 restriction sites. The on-target reporter plasmids contain a single site with

(97) A diagnostic circulating miRNA signature as orchestrator of cell invasion via TKS4/TKS5/EFHD2 modulation in human gliomasJournal of experimental & clinical cancer research : CRMarch 17, 2023Díaz Méndez AB, Sacconi A, Tremante E, Lulli V, Caprara V, Rosanò L, Goeman F, Carosi M, Di Giuliani M, Vari G, et al.and their mutants were cloned into pGL3 Control vector (#E1741, Promega) or into psiCHECK2 vector (#C8021, Promega) downstream of the luciferase gene. Human HEK-293 T (RRID: CVCL_4U22) cells were transiently

(98) Divalent siRNAs are bioavailable in the lung and efficiently block SARS-CoV-2 infectionProceedings of the National Academy of Sciences of the United States of AmericaMarch 14, 2023Hariharan VN, Shin M, Chang CW, O'Reilly D, Biscans A, Yamada K, Guo Z, Somasundaran M, Tang Q, Monopoli K, et al.to 20-nucleotide long, unique regions of target mRNAs were cloned into a psiCHECK-2 vector (Promega; C8021) according to the manufacturer′s protocol. HeLa cells were transfected with reporter plasmid using Lipofectamine

(99) Cancer stem cell-like cells-derived exosomal lncRNA CDKN2B-AS1 promotes biological characteristics in thyroid cancer via miR-122-5p/P4HA1 axisRegenerative TherapyMarch 1, 2023Qinghua Wu, Yonggang He, Xin Liu, Fangxiu Luo, Yimei Jiang, Ming Xiang, Ren Zhaomutant (MUT) reporter plasmid (CDKN2B-AS1-MUT or P4HA1-MUT) were constructed using psiCHECK-2 vector (C8021, Promega, USA). The sequences of miR-122-5p mimic (M) (miR10000421-1-5) and mimic control (MC) (miR1N0000001-1-5)

(100) miR579-3p is an inhibitory modulator of neointimal hyperplasia and transcription factors c-MYB and KLF4Cell death discoveryFebruary 22, 2023Xie X, Shirasu T, Li J, Guo LW, Kent KC.and PDGF-BB (520-BB) were from R&D Systems. Cell Titer-Glo 2.0 Assay kit (G9242), psiCHECK2 vector (C8021), and Dual-Luciferase Reporter Assay System (E1910) were from Promega. The following products were from

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