psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(201) Role of microRNA-122 in hepatic lipid metabolism of the weanling female rat offspring exposed to prenatal and postnatal caloric restrictionThe Journal of Nutritional BiochemistryNovember 1, 2019Yun Dai, Shubhamoy Ghosh, Bo-Chul Shin, Sherin U. Devaskar(transfection control) and the synthetic Renilla Luciferase (hRluc) gene (reporter) was obtained from Promega (C8021, Madison, WI). A complementary target site for the miR-122 seed sequence (5’-CAAACACCATTGTCACACTCCA-3’)

(202) PRC2 activates interferon-stimulated genes indirectly by repressing miRNAs in glioblastomaPLOS ONESeptember 12, 2019Haridha Shivram, Steven V. Le, Vishwanath R. Iyerleast 500 bp around the predicted miRNA binding sites were cloned into the psi-CHECK2 vector (Promega, C8021) downstream of the Renilla luciferase gene (S1 Table). Mutagenesis was performed using the QuikChange

(203) The RNA hairpin binder TRIM71 modulates alternative splicing by repressing MBNL1Genes & DevelopmentSeptember 1, 2019Thomas Welte, Alex C. Tuck, Panagiotis Papasaikas, Sarah H. Carl, Matyas Flemr, Philip Knuckles, Aneliya Rankova, Marc Bühler, Helge Großhansconstructs were generated by cloning respective 3′ UTRs into the NotI site of psiCHECK-2 (Promega, C8021). For generating pAAVS1 reporter constructs, sequences of unc-54 3′ UTR (pCM5.37; Addgene, 17253; gift

(204) Aberrantly expressed PLOD1 promotes cancer aggressiveness in bladder cancer: a potential prognostic marker and therapeutic targetMolecular oncologySeptember 1, 2019Yamada Y, Kato M, Arai T, Sanada H, Uchida A, Misono S, Sakamoto S, Komiya A, Ichikawa T, Seki N.between the SgfI and PmeI restriction sites of the 3′‐UTR of the hRluc gene within the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). We also generated PLOD1 3′‐UTR sequences containing deletions in the miR‐140‐5p

(205) miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and SurvivalCell reportsAugust 20, 2019Gagnon JD, Kageyama R, Shehata HM, Fassett MS, Mar DJ, Wigton EJ, Johansson K, Litterman AJ, Odorizzi P, Simeonov D, et al.mimic,Dharmacon,CN-001000-01-05 miR15b-5p miRIDIAN miRNA mimic,Dharmacon,C-310378-05-0002 Recombinant DNA,, Plasmid: psiCheck-2,Promega,C8021 Plasmid: psiCHECK-2-IL7r,In this study,NA Plasmid: psiCHECK-2-Cd28,In this study,NA Plasmid: siCHECK-2-Adrb2,In

(206) AGO CLIP Reveals an Activated Network for Acute Regulation of Brain Glutamate Homeostasis in Ischemic StrokeCell reportsJuly 23, 2019Kobayashi M, Benakis C, Anderson C, Moore MJ, Poon C, Uekawa K, Dyke JP, Fak JJ, Mele A, Park CY, et al.mm12146 pLenti-III-mir-GFP Control Vector,Applied Biological Materials,Cat# m001 psiCHECK-2 Vector,Promega,Cat# C8021 Software and Algorithms,Software and Algorithms,Software and Algorithms STAR 2.5.2,"Dobin et al.,

(207) miR‑28‑5p suppresses cell proliferation and weakens the progression of polycystic ovary syndrome by targeting prokineticin‑1Molecular Medicine ReportsJuly 1, 2019Lyuhe Meng, Haiyan Yang, Congcong Jin, Song Quanwild‑type (WT) and MUT 3'‑UTRs of PROK1 were then separately ligated into Psi‑CHECK2 plasmids (cat. no. C8021; Promega Corporation). Psi‑CHECK2 plasmids were co‑transfected with miR‑28‑5p mimics or NC into cells

(208) HNRNPA1-mediated 3′ UTR length changes of HN1 contributes to cancer- and senescence-associated phenotypesAging (Albany NY)June 30, 2019Qi Jia, Hongbo Nie, Peng Yu, Baiyun Xie, Chenji Wang, Fu Yang, Gang Wei, Ting NiUTR sequence was amplified from human genomic DNA and cloned into psiCHECK2 vector (Promega, cat. no. C8021) using the XhoI and PmeI restriction enzyme sites located at the 3′ end of the Renilla gene. All construct

(209) Mutant FUS and ELAVL4 (HuD) Aberrant Crosstalk in Amyotrophic Lateral SclerosisCell reportsJune 25, 2019De Santis R, Alfano V, de Turris V, Colantoni A, Santini L, Garone MG, Antonacci G, Peruzzi G, Sudria-Lopez E, Wyler E, et al.RRID: Addgene_65757 Plasmid epB-Puro-TT-RFP,"Rosa et al., 2014",N/A pSI-Check2 vector,Promega,Cat#C8021; RRID: Addgene_106979 Plasmid epB-Puro-TT-RFP-FUS,This paper,N/A Plasmid epB-Puro-TT-RFP-FUS-P525L,This

(210) Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axisMolecular medicine reportsJune 1, 2019Liu DW, Zhang YN, Hu HJ, Zhang PQ, Cui W.cat. no. 55704; Celprogen, Inc.) was cloned into a psiCHECK™-2 luciferase reporter vector (cat. no. C8021, Promega) to generate HIF-1α (WT). The mutant (MUT) version of the HIF-1α 3′-UTR lacking complementary

(211) Common Regulatory Pathways Mediate Activity of MicroRNAs Inducing Cardiomyocyte ProliferationCell reportsMay 28, 2019Torrini C, Cubero RJ, Dirkx E, Braga L, Ali H, Prosdocimo G, Gutierrez MI, Collesi C, Licastro D, Zentilin L, et al.pCSII-EF-MCS plasmid,"Gift from Stefano Piccolo, University of Padova",N/A psiCheck2.1 plasmid,Promega,Cat# C8021 3′UTR-Cfl2 plasmid,Biomatik,Clone ID T4545-1 3′UTR-β-TrC plasmid,Genewiz,Clone ID B28633-1/K28633 3′UTR-TAOK1

(212) Potential tumor‑suppressive role of microRNA‑99a‑3p in sunitinib‑resistant renal cell carcinoma cells through the regulation of RRM2International journal of oncologyMay 1, 2019Osako Y, Yoshino H, Sakaguchi T, Sugita S, Yonemori M, Nakagawa M, Enokida H.between the XhoI and PmeI restriction sites in the 3'‑UTR of the hRluc gene in a psiCHECK‑2 vector (C8021; Promega Corporation, Madison, WI, USA). ACHN, 786‑o, and SU‑R‑786‑o cells were transfected with 50

(213) Molecular Pathogenesis of Gene Regulation by the miR-150 Duplex: miR-150-3p Regulates TNS4 in Lung AdenocarcinomaCancers (Basel)April 30, 2019Shunsuke Misono et al.Dual Luciferase Reporter Assay The following two sequences were cloned into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA): the wild-type sequence of the 3’ untranslated regions (UTRs) of TNS4, or

(214) miR‑1271 enhances the sensitivity of colorectal cancer cells to cisplatinExperimental and Therapeutic MedicineApril 18, 2019Huixiang Yao, Qun Sun, Jinshui Zhu3′-UTR of mTOR were inserted downstream of the luciferase in the psiCHECK-2 reporter plasmid (cat. no. C8021; Promega Corporation, Madison, WI, USA). A deletion mutant lacking the seed sequence of the miR-1271

(215) Tumor‑suppressive microRNA‑223 targets WDR62 directly in bladder cancerInternational Journal of OncologyMarch 22, 2019Satoshi Sugita, Hirofumi Yoshino, Masaya Yonemori, Kazutaka Miyamoto, Ryosuke Matsushita, Takashi Sakaguchi, Toshihiko Itesako, Shuichi Tatarano, Masayuki Nakagawa, Hideki Enokidathe XhoI and PmeI restriction sites within the 3'‑UTR of the hRluc gene in the psiCHECK‑2 vector (P/N C8021; Promega, Madison, WI, USA). The T24 and BOY cells were transfected with 50 ng vector and 10 nM miR‑223

(216) Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 RegulationCancers (Basel)March 7, 2019Muhammad Khalid et al.and Dual Luciferase Reporter Assay The following 2 sequences were cloned into the psiCHECk-2 vector (C8021; Promega Corporation, Madison, WI, USA): the wild-type sequence of the 3′-untranslated regions (UTRs)

(217) Communication of prostate cancer cells with bone cells via extracellular vesicle RNA; a potential mechanism of metastasisOncogeneMarch 1, 2019C. Probert, T. Dottorini, A. Speakman, S. Hunt, T. Nafee, A. Fazeli, S. Wood, J. E. Brown, V. Jamescloning the miR21 seed sequences into the 3’-UTR of the psiCHECK2 dual luciferase reporter plasmid (#C8021, Promega, Southampton, UK). hOB cells were seeded into a 96-well plate at a density of 8 × 103 cells

(218) Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1bCell reportsFebruary 26, 2019Lin K, Qiang W, Zhu M, Ding Y, Shi Q, Chen X, Zsiros E, Wang K, Yang X, Kurita T, et al.paper,N/A V14–6 × His-SUMO,Laboratory of Ming Lei,N/A pUC57,Genewiz,N/A psiCHECK-2 Vector,Promega,Cat#C8021 "Chemicals, Reptides, and Recombinant Proteins","Chemicals, Reptides, and Recombinant Proteins","Chemicals,

(219) Regulation of KIF2A by Antitumor miR-451a Inhibits Cancer Cell Aggressiveness Features in Lung Squamous Cell CarcinomaCancers (Basel)February 22, 2019Akifumi Uchida, Naohiko Seki, Keiko Mizuno, Yasutaka Yamada, Shunsuke Misono, Hiroki Sanada, Naoko Kikkawa, Tomohiro Kumamoto, Takayuki Suetsugu, Hiromasa Inoue-UTR, either containing or lacking the miR-451a target site, were cloned into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). After cotransfecting miRNA and the constructed vector into EBC-1 and SK-MES-1

(220) Pirin: a potential novel therapeutic target for castration‐resistant prostate cancer regulated by miR‐455‐5pMolecular OncologyFebruary 1, 2019Takayuki Arai, Satoko Kojima, Yasutaka Yamada, Sho Sugawara, Mayuko Kato, Kazuto Yamazaki, Yukio Naya, Tomohiko Ichikawa, Naohiko Sekia sequence having a mutation in the miR‐455‐5p target site was inserted into the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). The assay procedure was reported previously (Arai et al., 2017, 2018b;

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