psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) microRNA-455-5p alleviates neuroinflammation in cerebral ischemia/reperfusion injuryNeural regeneration researchAugust 1, 2022Zhang JS, Hou PP, Shao S, Manaenko A, Xiao ZP, Chen Y, Zhao B, Jia F, Zhang XH, Mei QY, et al.and the respective mutant controls into the luciferase reporter plasmid PGL3 miReport vector (Cat# C8021, Promega, Madison, WI, USA). Human embryonic kidney 293T (HEK293T) cells (Cat# 12022001-1VL, RRID: CVCL_0063,

(122) Upregulation of β-catenin due to loss of miR-139 contributes to motor neuron death in amyotrophic lateral sclerosisStem Cell ReportsJuly 12, 2022Sophie Hawkins et al.resultant sequence was cloned downstream of the Renilla luciferase gene in the PSICheck2 vector (Promega C8021). To mutate the miR-139 binding site, we deleted positions 2, 4, and 6 of the predicted miRNA binding

(123) Identification of Antitumor miR-30e-5p Controlled Genes; Diagnostic and Prognostic Biomarkers for Head and Neck Squamous Cell CarcinomaGenesJuly 9, 2022Minemura C, Asai S, Koma A, Kikkawa N, Kato M, Kasamatsu A, Uzawa K, Hanazawa T, Seki N.sequence of FOXD1 within the 3′-untranslated region (3′-UTR), was inserted into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Alternatively, a deletion type that was missing the miR-30e-5p target site

(124) Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibitionNeuronJuly 6, 2022Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, Bischofberger J, Scheiffele P.study,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, Plasmid: psiCHECK-2,Promega,Cat# C8021 Plasmid: pAPtag-5,GenHunter,Cat# QV5 Plasmid: pDisplay-AP-CFP-TM,Alice Ting Lab,RRID: Addgene_20861 Plasmid:

(125) Self-delivering RNAi compounds as therapeutic agents in the central nervous system to enhance axonal regeneration after injuryiScienceJune 17, 2022Woller SA, Ruschel J, Morquette B, Cardia J, Yan D, Holton K, Shmushkovich T, Niederst E, Bulock K, Wolfson A, et al.reporter plasmid was constructed by inserting PTEN targeting region into psiCheck2 plasmid (Promega, Cat# C8021; Madison, WI) downstream of the Renilla luciferase sequence. MAP4K4 sdRNA sequence (Byrne et al., 2013)

(126) Cytosolic microRNA-inducible nuclear translocation of Cas9 protein for disease-specific genome modificationNucleic acids researchJune 10, 2022Shin CH, Park SC, Park IG, Kim H, An B, Lee C, Kim SH, Lee J, Lee JM, Oh SJ.sequence of target miRNA was inserted in multiple cloning regions of psiCHECK™-2 Vector (Promega, #C8021) using restriction enzyme Xho1 and EcoR1. The recombined miRNA target-psiCHECK™-2 vector was transfected

(127) METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenanceeLifeJune 8, 2022Matsuura-Suzuki E, Shimazu T, Takahashi M, Kotoshiba K, Suzuki T, Kashiwagi K, Sohtome Y, Akakabe M, Sodeoka M, Dohmae N, et al.E. coli Recombinant DNA reagent,pGL3 basic,Promega,Cat#E1751, Recombinant DNA reagent,psiCHECK2,Promega,Cat#C8021, Recombinant DNA reagent,psiCHECK2-Y0×,This study,,Encoding Rluc-Fluc fusion Recombinant DNA reagent,psiCHECK2-Y39×,This

(128) p53 Regulates a miRNA-Fructose Transporter Axis in Brown Adipose Tissue Under FastingFrontiers in GeneticsJune 6, 2022Isabel Reinisch et al.seed matches for miR-92a-1-5p was cloned in a PsiCheck2 vector (Promega, Madison, WI, United States, C8021). HEK293 cells were transfected with 1 µM mmu-miR-92-1-5p-mimic (Horizon discoveries, Waterbeach, United

(129) EIF4A3-induced circTOLLIP promotes the progression of hepatocellular carcinoma via the miR-516a-5p/PBX3/EMT pathwayJournal of experimental & clinical cancer research : CRMay 5, 2022Liu Y, Song J, Zhang H, Liao Z, Liu F, Su C, Wang W, Han M, Zhang L, Zhu H, et al.FLAG-pLenti-CMV-GFP-Puro. The dual-luciferase reporter vector psiCHECK™-2 Vector was purchased from Promega (C8021, USA) and psiCHECK-2-WT-circTOLLIP, psiCHECK-2-MUT-circTOLLIP, psiCHECK-2-WT-PBX3, and psiCHECK-2-MUT-PBX3

(130) TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancerNature communicationsApril 20, 2022Su Z, Monshaugen I, Wilson B, Wang F, Klungland A, Ougland R, Dutta A.luciferase reporter assay Dual luciferase reporter was constructed in psiCHECK-2 vector (Promega #C8021) to insert target sequence into the 3′ UTR of Renilla luciferase gene. tRF-3021bAla, tRF-3030bTyr and

(131) CFIm-mediated alternative polyadenylation remodels cellular signaling and miRNA biogenesisNucleic Acids ResearchApril 8, 2022Souvik Ghosh et al.OMERO client server web tool for generating figures. Luciferase assays psiCHECK-2 Vector (Promega; C8021) was digested at XhoI–NotI restriction sites for insertion of the binding regions for the miRNA targets

(132) Mutant FUS and ELAVL4 (HuD) Aberrant Crosstalk in Amyotrophic Lateral SclerosisSSRN Electronic JournalApril 1, 2022Riccardo De Santis et al.RRID: Addgene_65757 Plasmid epB-Puro-TT-RFP Rosa et al., 2014 N/A pSI-Check2 vector Promega Cat#C8021; RRID: Addgene_106979 Plasmid epB-Puro-TT-RFP-FUS This paper N/A Plasmid epB-Puro-TT-RFP-FUS-P525L This

(133) Identification of Tumor-Suppressive miR-30e-3p Targets: Involvement of SERPINE1 in the Molecular Pathogenesis of Head and Neck Squamous Cell CarcinomaInternational Journal of Molecular SciencesMarch 30, 2022Chikashi Minemura et al.of SERPINE1 with or without the miR-30e-3p-binding sites were inserted into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). The differences between wild-type and deletion type sequences are detailed

(134) PUMILIO proteins promote colorectal cancer growth via suppressing p21Nature communicationsMarch 25, 2022Gong Y, Liu Z, Yuan Y, Yang Z, Zhang J, Lu Q, Wang W, Fang C, Lin H, Liu S.instructions. Luciferase reporter assay The 3′UTR of p21 cDNA was amplified and cloned into psiCHECK2 vector (C8021, Promega) using primers specified in Supplementary Table 1. In the 3′UTR reporter assay, HCT116 cells

(135) Impact of miR-1/miR-133 Clustered miRNAs: PFN2 Facilitates Malignant Phenotypes in Head and Neck Squamous Cell CarcinomaBiomedicinesMarch 12, 2022Shunichi Asai, Ayaka Koma, Nijiro Nohata, Takashi Kinoshita, Naoko Kikkawa, Mayuko Kato, Chikashi Minemura, Katsuhiro Uzawa, Toyoyuki Hanazawa, Naohiko Sekisequences including or lacking predicted miRNA-binding sequence were inserted into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Transfection of the purified plasmid vectors into HNSCC cells were performed

(136) microRNA‐99a‐5p induces cellular senescence in gemcitabine‐resistant bladder cancer by targeting SMARCD1Molecular OncologyMarch 1, 2022Motoki Tamai et al.between the XhoI and PmeI restriction sites of the 3′‐UTR of the hRluc gene in the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). GEM‐R‐BOY and GEM‐R‐T24 cells were transfected with 50 ng of vector and

(137) circKL inhibits the growth and metastasis of kidney cancer by sponging miR‑182‑5p and upregulating FBXW7Oncology ReportsFebruary 17, 2022Juan Cao, Uet Yu, Li Li, Xiuli Yuan, Senmin Chen, Huanli Xu, Meng Yi, Sixi Liu(UTR) of FBXW7 were subcloned downstream of the luciferase gene using the psiCHEK-2 vector (cat. no. C8021; Promega Corporation). Sequences of primers used to amplify the targeted 3′-UTR of FBXW7 mRNA were as

(138) Competitive Endogenous RNA Network Activates Host Immune Response in SARS-CoV-2-, panH1N1 (A/California/07/2009)-, and H7N9 (A/Shanghai/1/2013)-Infected CellsCellsJanuary 30, 2022Minghui Yang et al.binding sites of lncRNA-34087.27 was conducted into a 3′UTR luciferase reporter psiCHECK2 plasmid (C8021, Promega, USA) named h-Lnc34087.27-WT; a mutant sequence of the plasmid of lncRNA- XLOC_098131 without

(139) CircRNA-011235 Counteracts The Deleterious Effect of Irradiation Treatment on Bone Mesenchymal Stem Cells by Regulating The miR-741-3p/CDK6 PathwayCell JJanuary 30, 2022Xianhui Wen, Hebin Xie, Rong Gui, Xinmin Nie, Dongyong Shan, Rong Huang, Hongyu Deng, Junhua Zhang(Shanghai, China). Subsequently, the fragments were subcloned into the psiCHECK-2 Renilla (Cat No. C8021, Promega, Madison, WI, USA) luciferase reporter vector following the manufacturer’s instructions. BMSCs

(140) Mitochondrial Ndufa4l2 Enhances Deposition of Lipids and Expression of Ca9 in the TRACK Model of Early Clear Cell Renal Cell CarcinomaFrontiers in OncologyDecember 14, 2021Kristian B. Laursen, Qiuying Chen, Francesca Khani, Nabeel Attarwala, Steve S. Gross, Lukas Dow, David M. Nanus, Lorraine J. GudasmNdufa4l2 (–)E4/mNdufa4l2(+)X1 primer-pair ( Table S1 ), and inserted into the psiCHECK2 vector (Cat# C8021, Promega, WI) immediately downstream of the LucR coding region. The resulting psiCHECK2 mNdufa4l2 constructs

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