psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) E2F1/CKS2/PTEN signaling axis regulates malignant phenotypes in pediatric retinoblastomaCell death & diseaseSeptember 12, 2022Chen M, Zhao Z, Wu L, Huang J, Yu P, Qian J, Ni T, Zhao C.TFs could bind to the promoter region of CKS2, a dual luciferase vector psiCHECK2 (Promega, cat. no. C8021) with CKS2’s wild-type (WT) or mutated promotors (Mut) was constructed. The promotor (WT or Mut) of

(122) SQSTM1/p62 promotes miR-198 loading into extracellular vesicles and its autophagy-related secretionHuman CellSeptember 1, 2022Xiaojie Yu, Hannah Eischeid-Scholz, Lydia Meder, Vangelis Kondylis, Reinhard Büttner, Margarete Odenthalaligned by CLC sequence viewer v6.0. Dual reporter luciferase assays The psiCheck™-2 plasmid (Promega, #C8021) harbors two expression cassette, driving expression of the Renilla and Firefly luciferase enzymes.

(123) Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturationNature communicationsAugust 30, 2022Hu W, Zeng H, Shi Y, Zhou C, Huang J, Jia L, Xu S, Feng X, Zeng Y, Xiong T, et al.previously described39. Briefly, the 3′-UTR of human OOSP2 was cloned into the psiCHECK2 vector (Promega, C8021) according to sequences downloaded from the NCBI website. The primers used are shown in Supplementary

(124) The silencing of ets-4 mRNA relies on the functional cooperation between REGE-1/Regnase-1 and RLE-1/Roquin-1Nucleic acids researchAugust 12, 2022Sobańska D, Komur AA, Chabowska-Kita A, Gumna J, Kumari P, Pachulska-Wieczorek K, Ciosk R.6. Dual-luciferase assay The 3′-UTRs were cloned into psiCheck-2 plasmid (Promega, USA, Cat. No. C8021) using the XhoI and NotI sites. The short fragment of the ets-4 3′-UTR (F1S) was inserted into the AscI

(125) microRNA-455-5p alleviates neuroinflammation in cerebral ischemia/reperfusion injuryNeural regeneration researchAugust 1, 2022Zhang JS, Hou PP, Shao S, Manaenko A, Xiao ZP, Chen Y, Zhao B, Jia F, Zhang XH, Mei QY, et al.and the respective mutant controls into the luciferase reporter plasmid PGL3 miReport vector (Cat# C8021, Promega, Madison, WI, USA). Human embryonic kidney 293T (HEK293T) cells (Cat# 12022001-1VL, RRID: CVCL_0063,

(126) Upregulation of β-catenin due to loss of miR-139 contributes to motor neuron death in amyotrophic lateral sclerosisStem Cell ReportsJuly 12, 2022Sophie Hawkins et al.resultant sequence was cloned downstream of the Renilla luciferase gene in the PSICheck2 vector (Promega C8021). To mutate the miR-139 binding site, we deleted positions 2, 4, and 6 of the predicted miRNA binding

(127) Identification of Antitumor miR-30e-5p Controlled Genes; Diagnostic and Prognostic Biomarkers for Head and Neck Squamous Cell CarcinomaGenesJuly 9, 2022Minemura C, Asai S, Koma A, Kikkawa N, Kato M, Kasamatsu A, Uzawa K, Hanazawa T, Seki N.sequence of FOXD1 within the 3′-untranslated region (3′-UTR), was inserted into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Alternatively, a deletion type that was missing the miR-30e-5p target site

(128) Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibitionNeuronJuly 6, 2022Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, Bischofberger J, Scheiffele P.study,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, Plasmid: psiCHECK-2,Promega,Cat# C8021 Plasmid: pAPtag-5,GenHunter,Cat# QV5 Plasmid: pDisplay-AP-CFP-TM,Alice Ting Lab,RRID: Addgene_20861 Plasmid:

(129) Self-delivering RNAi compounds as therapeutic agents in the central nervous system to enhance axonal regeneration after injuryiScienceJune 17, 2022Woller SA, Ruschel J, Morquette B, Cardia J, Yan D, Holton K, Shmushkovich T, Niederst E, Bulock K, Wolfson A, et al.reporter plasmid was constructed by inserting PTEN targeting region into psiCheck2 plasmid (Promega, Cat# C8021; Madison, WI) downstream of the Renilla luciferase sequence. MAP4K4 sdRNA sequence (Byrne et al., 2013)

(130) Cytosolic microRNA-inducible nuclear translocation of Cas9 protein for disease-specific genome modificationNucleic acids researchJune 10, 2022Shin CH, Park SC, Park IG, Kim H, An B, Lee C, Kim SH, Lee J, Lee JM, Oh SJ.sequence of target miRNA was inserted in multiple cloning regions of psiCHECK™-2 Vector (Promega, #C8021) using restriction enzyme Xho1 and EcoR1. The recombined miRNA target-psiCHECK™-2 vector was transfected

(131) METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenanceeLifeJune 8, 2022Matsuura-Suzuki E, Shimazu T, Takahashi M, Kotoshiba K, Suzuki T, Kashiwagi K, Sohtome Y, Akakabe M, Sodeoka M, Dohmae N, et al.E. coli Recombinant DNA reagent,pGL3 basic,Promega,Cat#E1751, Recombinant DNA reagent,psiCHECK2,Promega,Cat#C8021, Recombinant DNA reagent,psiCHECK2-Y0×,This study,,Encoding Rluc-Fluc fusion Recombinant DNA reagent,psiCHECK2-Y39×,This

(132) p53 Regulates a miRNA-Fructose Transporter Axis in Brown Adipose Tissue Under FastingFrontiers in GeneticsJune 6, 2022Isabel Reinisch et al.seed matches for miR-92a-1-5p was cloned in a PsiCheck2 vector (Promega, Madison, WI, United States, C8021). HEK293 cells were transfected with 1 µM mmu-miR-92-1-5p-mimic (Horizon discoveries, Waterbeach, United

(133) EIF4A3-induced circTOLLIP promotes the progression of hepatocellular carcinoma via the miR-516a-5p/PBX3/EMT pathwayJournal of experimental & clinical cancer research : CRMay 5, 2022Liu Y, Song J, Zhang H, Liao Z, Liu F, Su C, Wang W, Han M, Zhang L, Zhu H, et al.FLAG-pLenti-CMV-GFP-Puro. The dual-luciferase reporter vector psiCHECK™-2 Vector was purchased from Promega (C8021, USA) and psiCHECK-2-WT-circTOLLIP, psiCHECK-2-MUT-circTOLLIP, psiCHECK-2-WT-PBX3, and psiCHECK-2-MUT-PBX3

(134) TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancerNature communicationsApril 20, 2022Su Z, Monshaugen I, Wilson B, Wang F, Klungland A, Ougland R, Dutta A.luciferase reporter assay Dual luciferase reporter was constructed in psiCHECK-2 vector (Promega #C8021) to insert target sequence into the 3′ UTR of Renilla luciferase gene. tRF-3021bAla, tRF-3030bTyr and

(135) CFIm-mediated alternative polyadenylation remodels cellular signaling and miRNA biogenesisNucleic Acids ResearchApril 8, 2022Souvik Ghosh et al.OMERO client server web tool for generating figures. Luciferase assays psiCHECK-2 Vector (Promega; C8021) was digested at XhoI–NotI restriction sites for insertion of the binding regions for the miRNA targets

(136) Mutant FUS and ELAVL4 (HuD) Aberrant Crosstalk in Amyotrophic Lateral SclerosisSSRN Electronic JournalApril 1, 2022Riccardo De Santis et al.RRID: Addgene_65757 Plasmid epB-Puro-TT-RFP Rosa et al., 2014 N/A pSI-Check2 vector Promega Cat#C8021; RRID: Addgene_106979 Plasmid epB-Puro-TT-RFP-FUS This paper N/A Plasmid epB-Puro-TT-RFP-FUS-P525L This

(137) Identification of Tumor-Suppressive miR-30e-3p Targets: Involvement of SERPINE1 in the Molecular Pathogenesis of Head and Neck Squamous Cell CarcinomaInternational Journal of Molecular SciencesMarch 30, 2022Chikashi Minemura et al.of SERPINE1 with or without the miR-30e-3p-binding sites were inserted into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). The differences between wild-type and deletion type sequences are detailed

(138) PUMILIO proteins promote colorectal cancer growth via suppressing p21Nature communicationsMarch 25, 2022Gong Y, Liu Z, Yuan Y, Yang Z, Zhang J, Lu Q, Wang W, Fang C, Lin H, Liu S.instructions. Luciferase reporter assay The 3′UTR of p21 cDNA was amplified and cloned into psiCHECK2 vector (C8021, Promega) using primers specified in Supplementary Table 1. In the 3′UTR reporter assay, HCT116 cells

(139) Impact of miR-1/miR-133 Clustered miRNAs: PFN2 Facilitates Malignant Phenotypes in Head and Neck Squamous Cell CarcinomaBiomedicinesMarch 12, 2022Shunichi Asai, Ayaka Koma, Nijiro Nohata, Takashi Kinoshita, Naoko Kikkawa, Mayuko Kato, Chikashi Minemura, Katsuhiro Uzawa, Toyoyuki Hanazawa, Naohiko Sekisequences including or lacking predicted miRNA-binding sequence were inserted into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Transfection of the purified plasmid vectors into HNSCC cells were performed

(140) microRNA‐99a‐5p induces cellular senescence in gemcitabine‐resistant bladder cancer by targeting SMARCD1Molecular OncologyMarch 1, 2022Motoki Tamai et al.between the XhoI and PmeI restriction sites of the 3′‐UTR of the hRluc gene in the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). GEM‐R‐BOY and GEM‐R‐T24 cells were transfected with 50 ng of vector and

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