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Author Profile
Vincent Giacalone
Graduate Student
Emory University
Articles by Vincent Giacalone
Reliable Fc Block for Flow Cytometry of Human Cells
September 23, 2022
This reagent is used to block Fc receptors on cells for flow cytometry staining to prevent non-specific binding of antibodies.
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Great Cell Permeant Stain for Flow Cytometry
July 06, 2022
Nitric oxide is a member of the reactive nitrogen species important for a variety of functions including antimicrobial actions and...
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Versatile Compensation Beads for Flow Cytometry
April 27, 2022
These compensation beads are used to bind fluorescent antibodies to create single stain controls for flow cytometry.
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Time Course Effect of Chemoattractant on Fc Epsilon RI beta/MS4A2 Expression
April 27, 2022
IgE receptor Fc epsilon RI beta gene encodes the beta subunit of the high affinity family membrane-spanning 4A gene family. FCER1...
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Easy to Use Stain for Apoptosis
April 07, 2022
This reagent is used to identify cells undergoing apoptosis by staining for phosphatidylserine on the cell surface.
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Good Storage Buffer for Blood Samples
March 18, 2022
This reagent preserves stained blood samples for later analysis by flow cytometry. It's an ideal choice for collecting samples at ...
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Good RBC Lysis and Fixation Reagent
March 14, 2022
Whole blood samples were collected and stained for flow cytometry. The stable lyse reagent was used to lyse the erythrocytes and ...
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Good Antibody for TRPM8 Western Blot
March 07, 2022
Used antibody to test for the expression of TRPM8 in a new cell line compared to a validated cell line using western blot.
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Cell Permeant Stain for Calcium Flux
February 17, 2022
Blood neutrophils were cultured in either control condition or exposed to bacterial toxins that block calcium signaling...
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Reliable Antibody for T Cell Stimulation
February 03, 2022
PBMCs were cultured in a 96 well plate with anti-CD3 coated wells and proliferation was measured by flow cytometry.
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Great Dye for Tracking T Cell Proliferation
October 12, 2021
This dye was used to analyze T cell proliferation in different conditions. Cells are stained prior to culturing and lose half of ...
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Useful Metabolic Assay for Measuring ATP Production
July 28, 2021
Blood monocytes were isolated from 2 groups (drug treated and placebo) to determine if treatment had any effect on ATP production.
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Good Compensation Beads for Flow Cytometry Single Stains
July 07, 2021
Each tube of compensation beads is stained with one antibody from the staining panel. Create as many tubes of single-stained beads...
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Sensitive FRET Probe for Quantifying Activity of Extracellular Neutrophil Elastase
June 24, 2021
Bronchoalveolar lavage samples from cystic fibrosis patients measured for elastase activity.
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Seahorse Assay for Mitochondrial Function
June 16, 2021
Modulators of respiration are added to cells to assess mitochondrial function.
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Product Review
Reliable and Reproducible MTT Assay for Cell Viability Studies
Gherardo Baudo
MTT was prepared at 5 mg/mL in PBS, and 20 µL was added to each well containing 200 µL of medium (220 µL total volume). Cells were incubated for 4 hours at 37°C...
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