Good RBC Lysis and Fixation Reagent

Emory University
Pediatrics
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Smart Tube Inc.

Product Name:

Stable Lyse

Catalog Number:

STBLYSE2-250

Whole blood samples were collected and stained for flow cytometry. The stable lyse reagent was used to lyse the erythrocytes and fix the leukocytes.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Human whole blood

Tips

Adjust volume of reagent according to sample volume

Results Summary

This product was used to eliminate the red blood cells from each sample after staining and preserve the leukocytes. RBC lysis was complete as no red remained in the pellet following incubation and washing. The amount of debris remaining in the sample was acceptable as it did not interfere with any of the leukocyte populations. If desired, additional wash steps would likely further reduce debris presence.

DOI or PMID #

N/A

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Summary

The Good

Complete lysis of RBCs and not much debris present after washing.

The Bad

It is difficult to find specific details on the specific components of this reagent and their concentrations, info not readily available.

The Bottom Line

A good lysing and fixation reagent for flow cytometry.

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