Reliable Fc Block for Flow Cytometry of Human Cells

September 23, 2022

Emory University
Pediatrics
Graduate Student

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Quality of Results

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Company:

BioLegend

Product Name:

Human TruStain FcX

Catalog Number:

422302

This reagent is used to block Fc receptors on cells for flow cytometry staining to prevent non-specific binding of antibodies.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Whole blood or purified cells

Tips

Ensure that if staining for scavenger receptors, those antibody clones are compatible with this reagent.

Results Summary

This reagent is essential when staining for flow cytometry analysis. It will block Fc receptors on the cell surface to prevent non-specific binding of the staining antibodies, which is especially important for innate immune cell. In the example, neutrophils were stained for CD66b with or without the FcX and cells without FcX had much higher MFI (median fluorescence intensity).

DOI or PMID #

N/A

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Summary

The Good

Reliably prevents non specific antibody binding.

The Bad

May prevent the staining of certain Fc receptors, but certain clones are compatible.

The Bottom Line

This is an easy to use and essential reagent for flow cytometry as a pre-stain before antibody staining.

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