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Author Profile
Meng Sun
Lab Technician
Johns Hopkins School of Medicine
Articles by Meng Sun
Antibody User Review
Low Detection of Perforin on My cells of Interest
February 27, 2021
After our splenocyte harvest from mice infected with LCMV, unfortunately the staining for perforin was poor and could not detect ...
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Antibody User Review
Great Flow Staining for KLRG1
February 26, 2021
In order to gate out CD8s that are KLRG1+CD127- effector T cells, this antibody works well for us to look at memory versus ...
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Antibody User Review
Best for Genotyping
February 26, 2021
For our lab, we genotype most of our colony of mice. When we have multiple transgenic lines like P14, OTI, OTII, etc it is ...
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Assay Kit/Reagent User Review
Great for Looking at Cell Proliferation With Drug Treatment
February 26, 2021
Our lab uses multiple comparison of drugs that may inhibit cell proliferation therefore this dye helps us look at the amount of ...
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Assay Kit/Reagent User Review
Great Dye to Look at Cell Proliferation
February 26, 2021
Our lab uses cell proliferation dyes to label cells before our experiment and then we can track the cell division cycles (as the ...
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Antibody User Review
Great Flow Antibody for Looking at IL2
February 22, 2021
Cytokine production is normally a phenotypic marker of murine T cells. IL-2 specifically is normally one of our lab's top 3 ...
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Antibody User Review
Chemokine Expression Upon Activation
December 15, 2020
Upon T cell activation, there is normally an increase in expression of CXCR3. This increase can help us see the effector CD8 cells...
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Antibody User Review
Great Antibody for CD127 Detection
December 11, 2020
By using CD127 antibody, we can look at memory precursor cells in our population of interest, specifically CD8s. Naive and memory-...
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Antibody User Review
Great Way to Gate Out the Thy1.1 and Thy1.2 Cells
November 26, 2020
Our lab likes to use this combination, Thy1.2 in BV510, so that we can easily distinguish Thy1.1 and Thy1.2 cells. When we ...
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Antibody User Review
Great Way to Look at CD27 Expression on CD8s
November 26, 2020
Our lab is interested in murine CD8s and CD4s therefore using this antibody to look at lymphocytes specifically expressing high or...
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Antibody User Review
Great Way to Look at Affinity for IL-7 Differentiation
November 25, 2020
Our lab is interested in looking at response of T cells after any type of acute infection. For flow, we would like to look at ...
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Antibody User Review
Relatively Dim Antibody for Granzyme B
November 25, 2020
Since our lab was interested in looking at T cells, specifically CD8 and CD4, this molecule was of interest due to its expression ...
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Antibody User Review
Great IFNg antibody in PerCP
November 21, 2020
When our lab does adoptive transfer cells into a host and then infect the host with an acute infection (like LCMV Armstrong). When...
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Antibody User Review
Memory Marker on CD8
November 20, 2020
CD122 is normally used as a memory marker for our CD8 T cells. Our lab is interested in looking at the persistence of memory cell ...
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Antibody User Review
Great TNFa Signal on Flow Cytometry
November 20, 2020
Our lab is interested in looking at cytokine production upon re-stimulation of T cells after harvesting from spleens of infected ...
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Efficient and Cost Friendly SYBR Green Mastermix for qPCR
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I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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