Low Detection of Perforin on My cells of Interest

February 27, 2021

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse Perforin Antibody

Catalog Number:

154306

Clone Number:

S16009A

After our splenocyte harvest from mice infected with LCMV, unfortunately the staining for perforin was poor and could not detect much signal.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T cells

Primary Incubation

1:400 in FACS buffer

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Celesta

Results Summary

The perforin staining didn't show much signal on our lymphocytes. Although we know there there should be some level of cytotoxicity, we were not able to detect much.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The dilution was easy to titrate

The Bad

It didn't work very well for the nature of our experiment/cell of interest.

The Bottom Line

There might be better antibodies out there but our nature of our experiment might have altered how effective this antibody is.

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