Michaela Rekow

Research Technologist
Medical College of Wisconsin

Articles by Michaela Rekow

  • Antibody User Review

    Great CD8a Antibody for Flow Cytometry

    August 19, 2016
    Mice are infected with virus. Splenocytes are collected, RBC lysed, Fc blocked, fixed, and stained for flow cytometry. read more
  • Antibody User Review

    Great CD8a Flow Cytometry Antibody

    August 18, 2016
    Mice are infected with virus. Splenocytes are prepared using RBC lysis, Fc block, and fixing before flow cytometry. read more
  • Antibody User Review

    Good IgD Flow Cytometry Antibody

    August 17, 2016
    Mice are latently infected with virus. Splenocytes are prepared and stained for flow cytometry. read more
  • Antibody User Review

    ATM Antibody Review

    March 23, 2016
    This antibody was used for assessing ATM levels in bone marrow derived macrophages in virus infected mice. read more
  • Antibody User Review

    HDAC-2 Antibody Review

    March 16, 2016
    Infected or non-infected primary murine macrophages are western blotted for various proteins. read more
  • Antibody User Review

    HDAC-1 Antibody

    March 15, 2016
    This product was used for Western blotting on infected or noninfected primary murine macrophages. read more
  • Antibody User Review

    B-Actin Antibody Review

    March 11, 2016
    Infected or uninfected primary macrophages are western blotted for various proteins using b-Actin as a control. read more
  • Antibody User Review

    CD38 Antibody Review

    February 05, 2016
    Mice are latently or lytically infected. Slenocytes are harvested, RBC lysed, stained, and fixed. read more
  • Antibody User Review

    IgM Antibody Review

    February 03, 2016
    Mice are latently or lytically infected with virus. Splenocytes are collected and RBC lysed before staining and fixing for flow. read more
  • Antibody User Review

    CD44 PE Antibody Review

    February 02, 2016
    Mice are infected with virus. Spleens are collected, cells are RBC lysed, Fc blocked, stained, and washed before fixing and ... read more
  • Antibody User Review

    Great B220/CD45R Antibody

    February 01, 2016
    Mice are infected and splenocytes are collected, RBC lysed, Fc blocked, stained, and washed before performing flow. This antibody ... read more
  • Antibody User Review

    CD25 APC Antibody Review

    January 29, 2016
    Splenocytes are harvested from latently infected mice. They are RBC lysed, Fc blocked, stained for 30 minutes, and washed 2x ... read more
  • Equipment User Review

    Good Centrifuge

    January 28, 2016
    We use our centrifuge all the time for immunology and virology related assays. read more
  • Equipment User Review

    Great Electrophoresis System

    December 18, 2015
    We use our gel system to run gels for genotyping and LDPCR. They are used continuously every day. Use Agarose gel and TAE buffer. ... read more
  • Equipment User Review

    Fantastic Thermal Cycler

    December 03, 2015
    We use our Thermal Cyclers for genotyping PCR reactions and LDPCR. Most protocols run between 2-3 hours. read more
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