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Author Profile
Michaela Rekow
Research Technologist
Medical College of Wisconsin
Articles by Michaela Rekow
Antibody User Review
Great CD8a Antibody for Flow Cytometry
August 19, 2016
Mice are infected with virus. Splenocytes are collected, RBC lysed, Fc blocked, fixed, and stained for flow cytometry.
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Antibody User Review
Great CD8a Flow Cytometry Antibody
August 18, 2016
Mice are infected with virus. Splenocytes are prepared using RBC lysis, Fc block, and fixing before flow cytometry.
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Antibody User Review
Good IgD Flow Cytometry Antibody
August 17, 2016
Mice are latently infected with virus. Splenocytes are prepared and stained for flow cytometry.
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Antibody User Review
ATM Antibody Review
March 23, 2016
This antibody was used for assessing ATM levels in bone marrow derived macrophages in virus infected mice.
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Antibody User Review
HDAC-2 Antibody Review
March 16, 2016
Infected or non-infected primary murine macrophages are western blotted for various proteins.
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Antibody User Review
HDAC-1 Antibody
March 15, 2016
This product was used for Western blotting on infected or noninfected primary murine macrophages.
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Antibody User Review
B-Actin Antibody Review
March 11, 2016
Infected or uninfected primary macrophages are western blotted for various proteins using b-Actin as a control.
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Antibody User Review
CD38 Antibody Review
February 05, 2016
Mice are latently or lytically infected. Slenocytes are harvested, RBC lysed, stained, and fixed.
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Antibody User Review
IgM Antibody Review
February 03, 2016
Mice are latently or lytically infected with virus. Splenocytes are collected and RBC lysed before staining and fixing for flow.
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Antibody User Review
CD44 PE Antibody Review
February 02, 2016
Mice are infected with virus. Spleens are collected, cells are RBC lysed, Fc blocked, stained, and washed before fixing and ...
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Antibody User Review
Great B220/CD45R Antibody
February 01, 2016
Mice are infected and splenocytes are collected, RBC lysed, Fc blocked, stained, and washed before performing flow. This antibody ...
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Antibody User Review
CD25 APC Antibody Review
January 29, 2016
Splenocytes are harvested from latently infected mice. They are RBC lysed, Fc blocked, stained for 30 minutes, and washed 2x ...
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Equipment User Review
Good Centrifuge
January 28, 2016
We use our centrifuge all the time for immunology and virology related assays.
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Equipment User Review
Great Electrophoresis System
December 18, 2015
We use our gel system to run gels for genotyping and LDPCR. They are used continuously every day. Use Agarose gel and TAE buffer. ...
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Equipment User Review
Fantastic Thermal Cycler
December 03, 2015
We use our Thermal Cyclers for genotyping PCR reactions and LDPCR. Most protocols run between 2-3 hours.
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Product Review
Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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